血清miR-130a、miR-425-5p表达水平与宫颈癌患者临床病理参数的关系及其诊断价值分析
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摘要
目的探讨宫颈癌患者血清微小核糖核酸-130a (miR-130a)、微小核糖核酸-425-5p (miR-425-5p)的表达水平及与患者临床病理参数的关系,分析血清miR-130a、miR-425-5p对宫颈癌的诊断价值。方法选择2015年2月-2018年1月该院收治的癌前病变患者450例为研究对象,根据最终诊断结果以66例宫颈癌患者为观察组,另外384例为对照组。采用定量即时聚合酶链锁反应(QRT-PCR)法检测两组患者血清miR-130a、miR-425-5p表达水平,分析其表达水平与宫颈癌患者临床病理参数的关系及其对宫颈癌的早期诊断价值。结果观察组治疗前血清miR-130a、miR-425-5p的表达水平高于对照组(P<0. 05);观察组治疗后5～7 d的血清miR-130a、miR-425-5p表达水平与治疗前比较显著降低(P<0. 05);宫颈癌患者血清miR-130a、miR-425-5p的表达水平与年龄、肿瘤直径、病理类型无关(P>0. 05);与组织分化程度、临床分期和淋巴结转移情况有关(P<0. 05);血清miR-130a诊断宫颈癌的曲线下面积为0. 889,灵敏度为86. 36%,特异度为90. 91%;血清miR-425-5p诊断宫颈癌的曲线下面积为0. 913,灵敏度为89. 39%,特异度为93. 94%。结论宫颈癌患者血清miR-130a、miR-425-5p表达水平升高,表达水平与癌细胞分化程度、临床分期及是否有淋巴结转移有关,检测其表达水平对宫颈癌具有较高的诊断价值。
Objective To explore the relationships between serum miR-130 a and miR-425-5 p expression levels and clinicopathological parameters of cervical cancer patients,analyze the diagnostic value of serum miR-130 a and miR-425-5 p for cervical cancer.Methods From February 2015 to January 2018,450 cases of cervical precancerous lesion treated in Yichun People's Hospital were selected as study object,then they were divided into observation group( 66 cervical cancer cases) and control group( 384 cases) according to the final diagnostic result. QRT-PCR was used to detect the expression levels of serum miR-130 a and miR-425-5 p in the two groups. The relationships between expression levels of serum miR-130 a and miR-425-5 p and clinicopathological parameters of cervical cancer patients and the diagnostic value of serum miR-130 a and miR-425-5 p for cervical cancer were analyzed. Results The expression levels of serum miR-130 a and miR-425-5 p before treatment in observation group were statistically significantly higher than those in control group( P <0. 05). In observation group,the expression levels of serum miR-130 a and miR-425-5 p at 5-7 days after treatment were statistically significantly lower than those before treatment( P<0. 05). The expression levels of serum miR-130 a and miR-425-5 p in cervical cancer patients were not correlated with age,tumor diameter,and pathological type( P>0. 05). The expression levels of serum miR-130 a and miR-425-5 p in cervical cancer patients were correlated with histological differentiation,clinical stage,and lymph node metastasis( P<0. 05).The area under ROC curve of serum miR-130 a for diagnosis of cervical cancer was 0. 889,the sensitivity was 86. 36%,the specificity was90. 91%; the area under ROC curve of serum miR-425-5 p for diagnosis of cervical cancer was 0. 913,the sensitivity was 89. 39%,the specificity was 93. 94%. Conclusion The expression levels of serum miR-130 a and miR-425-5 p in patients with cervical cancer are high,which are correlated with the degree of tumor differentiation,clinical stage,and lymph node metastasis. The detection of expression levels of serum miR-130 a and miR-425-5 p is of high diagnostic value for cervical cancer.
Objective To explore the relationships between serum miR-130 a and miR-425-5 p expression levels and clinicopathological parameters of cervical cancer patients,analyze the diagnostic value of serum miR-130 a and miR-425-5 p for cervical cancer.Methods From February 2015 to January 2018,450 cases of cervical precancerous lesion treated in Yichun People's Hospital were selected as study object,then they were divided into observation group( 66 cervical cancer cases) and control group( 384 cases) according to the final diagnostic result. QRT-PCR was used to detect the expression levels of serum miR-130 a and miR-425-5 p in the two groups. The relationships between expression levels of serum miR-130 a and miR-425-5 p and clinicopathological parameters of cervical cancer patients and the diagnostic value of serum miR-130 a and miR-425-5 p for cervical cancer were analyzed. Results The expression levels of serum miR-130 a and miR-425-5 p before treatment in observation group were statistically significantly higher than those in control group( P <0. 05). In observation group,the expression levels of serum miR-130 a and miR-425-5 p at 5-7 days after treatment were statistically significantly lower than those before treatment( P<0. 05). The expression levels of serum miR-130 a and miR-425-5 p in cervical cancer patients were not correlated with age,tumor diameter,and pathological type( P>0. 05). The expression levels of serum miR-130 a and miR-425-5 p in cervical cancer patients were correlated with histological differentiation,clinical stage,and lymph node metastasis( P<0. 05).The area under ROC curve of serum miR-130 a for diagnosis of cervical cancer was 0. 889,the sensitivity was 86. 36%,the specificity was90. 91%; the area under ROC curve of serum miR-425-5 p for diagnosis of cervical cancer was 0. 913,the sensitivity was 89. 39%,the specificity was 93. 94%. Conclusion The expression levels of serum miR-130 a and miR-425-5 p in patients with cervical cancer are high,which are correlated with the degree of tumor differentiation,clinical stage,and lymph node metastasis. The detection of expression levels of serum miR-130 a and miR-425-5 p is of high diagnostic value for cervical cancer.
引文
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[11]Zununi Vahed S,Poursadegh Zonouzi A,Mahmoodpoor F,et al.Circulating mi R-150,mi R-192,mi R-200b,and mi R-423-3p as Non-invasive Biomarkers of Chronic Allograft Dysfunction[J].Arch Med Res,2017,48(1):96-104.
[12]Sun L,Jiang R,Li J,et al.Mico RNA-425-5p is a potential prognostic biomarker for cervical cancer[J].Ann Clin Biochem,2017,54(1):127-133.
[13]周争光,汪蕊,李玉梅,等.mir-130a-3p及smad 4在肝细胞癌组织中的表达及临床意义[J].安徽医科大学学报,2017,52(3):383-387.
[14]蔡洲,戴宇翃.mi RNA-425-5p促进胰腺癌细胞增殖、迁移与侵袭过程的分子机制[J].中国老年学杂志,2017,37(11):2629-2631.
[15]宋霞,毛熙光.Micro RNA-182在宫颈癌中的表达及对肿瘤细胞增殖、侵袭及迁移的影响[J].中国现代医学杂志,2017,27(14):48-52.
[2]祝莉,曲丽霞,权丽丽,等.宫颈癌盆腔淋巴结转移的临床特征及影响因素分析[J].癌症进展,2017,15(9):1079-1081.
[3]王捷敏.表观遗传调控在巨噬细胞极化中的研究进展[J].检验医学与临床,2018,15(2):277-280.
[4]钟阿红,郁春晴,董春林,等.mi R-124在消化系统肿瘤研究中的进展[J].现代生物医学进展,2016,16(9):1759-1761,1741.
[5]Grieco FA,Sebastiani G,Juan-Mateu J,et al.Micro RNAs mi R-23a-3p,mi R-23b-3p,and mi R-149-5p Regulate the Expression of Proapoptotic BH3-Only Proteins DP5 and PUMA in Human Pancreaticβ-Cells[J].Diabetes,2017,66(1):100-112.
[6]周颖,陈纲,徐菲,等.妊娠合并子宫颈癌的诊断与治疗进展[J].中华妇产科杂志,2016,51(7):555-558.
[7]魏小丽,顾康生,高梦如.Micro RNA与肿瘤铂类耐药的研究[J].中国药理学通报,2017,33(5):593-597.
[8]Panwar B,Omenn GS,Guan Y.mi Rmine:a database of human mi RNA expression profiles[J].Bioinformatics,2017,33(10):1554-1560.
[9]Okato A,Arai T,Kojima S,et al.Dual strands of pre-mi R-150(mi R-150-5p and mi R-150-3p)act as antitumor mi RNAs targeting SPOCK1 in na6ve and castration-resistant prostate cancer[J].Int J Oncol,2017,51(1):245-256.
[10]Osako Y,Seki N,Koshizuka K,et al.Regulation of SPOCK1 by dual strands of pre-mi R-150 inhibit cancer cell migration and invasion in esophageal squamous cell carcinoma[J].J Hum Genet,2017,62(11):935-944
[11]Zununi Vahed S,Poursadegh Zonouzi A,Mahmoodpoor F,et al.Circulating mi R-150,mi R-192,mi R-200b,and mi R-423-3p as Non-invasive Biomarkers of Chronic Allograft Dysfunction[J].Arch Med Res,2017,48(1):96-104.
[12]Sun L,Jiang R,Li J,et al.Mico RNA-425-5p is a potential prognostic biomarker for cervical cancer[J].Ann Clin Biochem,2017,54(1):127-133.
[13]周争光,汪蕊,李玉梅,等.mir-130a-3p及smad 4在肝细胞癌组织中的表达及临床意义[J].安徽医科大学学报,2017,52(3):383-387.
[14]蔡洲,戴宇翃.mi RNA-425-5p促进胰腺癌细胞增殖、迁移与侵袭过程的分子机制[J].中国老年学杂志,2017,37(11):2629-2631.
[15]宋霞,毛熙光.Micro RNA-182在宫颈癌中的表达及对肿瘤细胞增殖、侵袭及迁移的影响[J].中国现代医学杂志,2017,27(14):48-52.