Rho/ROCK信号通路与脑动脉硬化患者血液流变学的相关性研究
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摘要
目的探讨Rho/ROCK信号通路与脑动脉硬化(CAS)患者血液流变学之间的关系。方法选择CAS患者80例及健康体检者80例,收集血液样本,检测相应生物化学指标、Ras同族体基因家族成员A(RhoA)、Rho激酶2(ROCK2)水平及血细胞比容、全血表观黏度(高切、低切)、血浆黏度。比较2组血液流变学指标、RhoA、ROCK2水平;分析CAS患者RhoA、ROCK2水平与血液流变学指标的相关性。结果 CAS组吸烟比例、体质指数、空腹血糖、餐后2 h血糖、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、甘油三酯、血清同型半胱氨酸均高于对照组(P<0.05)。CAS组血细胞比容、全血表观黏度(高切、低切)、血浆黏度、ROCK2、RhoA水平均高于对照组(P <0.05)。Pearson相关分析及多重线性回归分析显示,ROCK2水平与血细胞比容、全血表观黏度高切、低切、血浆黏度呈正相关; RhoA水平与全血表观黏度(高切、低切)呈正相关。结论 Rho/ROCK信号通路与CAS患者血液流变学的异常密切相关,可能参与了CAS的发生发展。
Aim To investigate the relationship between Rho/ROCK signal pathway and hemorheology in patients with cerebral arteriosclerosis(CAS).Methods Blood samples were collected from 80 CAS patients and 80 healthy persons.The biochemical parameters,the levels of Ras homolog gene family member A(RhoA) and Rho-associated kinase 2(ROCK2),blood cell-specific volume,whole blood apparent viscosity(high shear and low shear) and plasma viscosity were measured.Hemorheological indexes,RhoA and ROCK2 levels were compared between the two groups.The correlations between RhoA,ROCK2 levels and hemorheological parameters were analyzed in CAS patients.Results The smoking rate,body mass index,fasting plasma glucose,2-hour postprandial plasma glucose,high density lipoprotein cholesterol,low density lipoprotein cholesterol,triglyceride and serum homocysteine in CAS group were higher than those in control group(P<0.05).The blood cell-specific volume,whole blood apparent viscosity(high shear and low shear),plasma viscosity,ROCK2 and RhoA levels in CAS group were higher than those in control group(P<0.05).Pearson correlation analysis and multiple linear regression analysis showed that serum ROCK2 level was positively correlated with blood cell-specific volume,whole blood apparent viscosity(high shear and low shear) and plasma viscosity;RhoA level was positively correlated with whole blood apparent viscosity(high shear and low shear).Conclusion Rho/ROCK signal pathway is closely related to abnormal hemorheology in CAS patients,and it may be involved in the occurrence and development of CAS.
Aim To investigate the relationship between Rho/ROCK signal pathway and hemorheology in patients with cerebral arteriosclerosis(CAS).Methods Blood samples were collected from 80 CAS patients and 80 healthy persons.The biochemical parameters,the levels of Ras homolog gene family member A(RhoA) and Rho-associated kinase 2(ROCK2),blood cell-specific volume,whole blood apparent viscosity(high shear and low shear) and plasma viscosity were measured.Hemorheological indexes,RhoA and ROCK2 levels were compared between the two groups.The correlations between RhoA,ROCK2 levels and hemorheological parameters were analyzed in CAS patients.Results The smoking rate,body mass index,fasting plasma glucose,2-hour postprandial plasma glucose,high density lipoprotein cholesterol,low density lipoprotein cholesterol,triglyceride and serum homocysteine in CAS group were higher than those in control group(P<0.05).The blood cell-specific volume,whole blood apparent viscosity(high shear and low shear),plasma viscosity,ROCK2 and RhoA levels in CAS group were higher than those in control group(P<0.05).Pearson correlation analysis and multiple linear regression analysis showed that serum ROCK2 level was positively correlated with blood cell-specific volume,whole blood apparent viscosity(high shear and low shear) and plasma viscosity;RhoA level was positively correlated with whole blood apparent viscosity(high shear and low shear).Conclusion Rho/ROCK signal pathway is closely related to abnormal hemorheology in CAS patients,and it may be involved in the occurrence and development of CAS.
引文
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[13]毛江洪,汪青山,钮心怡,等.临床血液流变学的研究现状[J].中国优生与遗传杂志,2013,21(5):148-151.
[14]武多娇,张怡,高平进,等.法舒地尔抗动脉粥样硬化作用及其机制[J].中国临床药学杂志,2010,19(2):69-74.
[2]王玉珍,何奕涛.经颅多普勒超声对脑动脉硬化症诊断价值研究[J].中国实用神经疾病杂志,2013,16(9):17-19.
[3]李南南,张军平,仲爱芹,等.Rho A/ROCK信号通路在动脉粥样硬化发生中的作用[J].基础医学与临床,2014,34(10):1422-1425.
[4]Noma K,Oyama N,Liao JK.Physiological role of ROCKs in the cardiovascular system[J].Am J Physiol Cell Physiol,2006,290(3):C661-C668.
[5]陈剑飞.脑动脉硬化与血脂及血液流变学关系的研究[D].重庆:重庆医科大学,2010;9.
[6]史玉泉.实用神经病学[M].第3版.上海:上海科学技术出版社,2004;954-956.
[7]Chen ZY,Yao WJ.Role of Rho/ROCK in the migration of vascular smooth muscle cells[J].Sheng Li Ke Xue Jin Zhan,2013,44(4):269-274.
[8]Li N,Chen J,Zhao J,et al.MicroRNA-3188 targets ETS-domain protein 4 and participates in Rho A/ROCK pathway to regulate the development of atherosclerosis[J].Pharmazie,2017,72(11):687-693.
[9]韩佳寅,易艳,梁爱华,等.Rho/ROCK信号通路研究进展[J].药学学报,2016,51(6):853-859.
[10]Amano M,Nakayama M,Kaibuchi K.Rho-kinase/ROCK:A key regulator of the cytoskeleton and cell polarity[J].Cytoskeleton(Hoboken),2010,67(9):545-554.
[11]Zhou Q,Gensch C,Liao JK.Rho-associated coiled-coilforming kinases(ROCKs):potential targets for the treatment of atherosclerosis and vascular disease[J].Trends Pharmacol Sci,2011,32(3):167-173.
[12]韩星伟.慢性缺血性脑血管病与脑动脉硬化性狭窄的关系[J].中国实用神经疾病杂志,2017,20(11):130-131.
[13]毛江洪,汪青山,钮心怡,等.临床血液流变学的研究现状[J].中国优生与遗传杂志,2013,21(5):148-151.
[14]武多娇,张怡,高平进,等.法舒地尔抗动脉粥样硬化作用及其机制[J].中国临床药学杂志,2010,19(2):69-74.