BSA法测定超滤膜截留率的不确定度评定
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摘要
截留性能是超滤膜重要的性能指标,截留率和切割分子量又是表征超滤膜截留性能的主要技术参数。基于GB/T 32360—2015《超滤膜测试方法》,依据JJF 1059—2012《测量不确定度评定与表示》,以牛血清白蛋白(BSA-67000)为标准物质,建立截留率测试结果的不确定度评定数学模型,全面分析不确定度来源,评定超滤膜截留率测试结果的不确定度。结果表明:BSA-67000的多分散系数(Mw/(Mn)为1. 101,GPC谱图呈现单峰形式,结构明确、分子量分布窄,完全满足测试需要。对该结果进行了不确定度评定,当包含因子k=2,置信水平为95%时,扩展不确定度为7. 86%,测试结果的不确定度主要来源于测试液取样、重复性测试和BSA-67000的紫外分光光度法测定。
Retention performance is the most important performance indicator of ultrafiltration membrane performance,including two technical parameters which are molecular weight cut off and retention. Based on GB/T 32360—2015 " Test Methods for Ultrafiltration Membrane",according to the JJF 1059—2012 " Evaluation and Expression of Uncertainty in Measurement",BSA-67000 was choosed as standard substance,evaluation mathematical model of uncertainty of retention test results was established to analyze the sources of uncertainty,and the retention test results were evaluated. The results indicated that the polydispersity coefficient( Mw/( Mn) of BSA-67000 is 1. 101 and GPC spectrum showed a single peak form,in line with PEG clear structure,molecular weight distribution narrow features,which fully meet the testing needs. The results were evaluated uncertainty. It showed that expanded uncertainty is 7. 86%,when coverage factor is k = 2 and level of confidence is 95%. Uncertainty of the measurement results were mainly from the removing the test sample,repeatability and spectrophotometric determination of BSA-67000.
Retention performance is the most important performance indicator of ultrafiltration membrane performance,including two technical parameters which are molecular weight cut off and retention. Based on GB/T 32360—2015 " Test Methods for Ultrafiltration Membrane",according to the JJF 1059—2012 " Evaluation and Expression of Uncertainty in Measurement",BSA-67000 was choosed as standard substance,evaluation mathematical model of uncertainty of retention test results was established to analyze the sources of uncertainty,and the retention test results were evaluated. The results indicated that the polydispersity coefficient( Mw/( Mn) of BSA-67000 is 1. 101 and GPC spectrum showed a single peak form,in line with PEG clear structure,molecular weight distribution narrow features,which fully meet the testing needs. The results were evaluated uncertainty. It showed that expanded uncertainty is 7. 86%,when coverage factor is k = 2 and level of confidence is 95%. Uncertainty of the measurement results were mainly from the removing the test sample,repeatability and spectrophotometric determination of BSA-67000.
引文
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[17] Arkhangelsky E,Duek A,Gitis V. Maximal pore size in UF membranes[J]. Journal of Membrane Science,2012,394-395:89-97.
[18]张激光.分光光度法测定地质样品中MO的不确定度评定[J].计量学报,2013,34(2):196-200.Zhang J G. Uncertainty Evaluation for the Determination of Mo in Geological Samples by Spectrophotometry[J].Acta Metrologica Sinica,2013,34(2):196-200.
[19]罗嫣,张晓慧,石超英.检测条件对中空纤维超滤膜截留率的影响研究[J].广州化工,2015,43(14):123-124,133.Luo Y,Zhang X H,Shi C Y. Analysis of the Influence of Test Conditions on the Rejection of Hollow Fiber Ultrafiltration Membranes[J]. Guang Zhou Chemical Industry and Technology,2015,43(14):123-124,133.
[20]王旭亮,李宗雨,张艳萍,等.聚乙二醇法测定超滤膜截留率的不确定度评[J].计量学报,2018,39(1):119-124.Wang X L,Li Z Y,Zhang Y P,et al. Uncertainty Evaluation for the Determination of the Retention Ratio of the Ultrafiltration Membrane by PEG-20000[J].Acta Metrologica Sinica,2018,39(1):119-124.
[2]倪澄峰,安树林,邓利华,等. D试剂法超滤膜截留聚乙二醇的研究[J].膜科学与技术,2016,36(2):109-113.Ni C F,An S L,Deng L H,et al. Discussion about ultrafiltration membranes retention performance by D reagent method[J]. Membrane Science and Technology,2016,36(2):109-113.
[3] Kader G. A Large Review of the Pre Treatment[J].Expanding issues in desalination,2011,(9):1-54.
[4] Rohani R, Hyland M, Patterson D. A refined onefiltration method for aqueous based nanofiltration and ultrafiltration membrane molecular weight cut-off determination using polyethylene glycols[J]. Jornal of Membrane Science,2011,382(1),278-290.
[5]潘献辉,王晓楠,张艳萍,等.超滤膜截留性能检测用基准物质研究与应用进展[J].膜科学与技术,2013,33(2):104-108.Pan X H,Wang X N,Zhang YP,et al. The progress on standard substance used for determining the retention performance of ultrafiltration membrane[J]. Membrane Science and Technology,2013,33(2):104-108.
[6] Calvo J I,Peinador R I,Prdanos P,et al. Liquid-liquid displacement porometry to estimate the molecular weight cut-off of ultrafiltration membranes[J]. Desalination,2011,268(1),174-181.
[7]祝振鑫.关于推荐使用“切割分子量”一词的建议[J].膜科学与技术,2006,26(2):82-83.Zhu Z X. Suggestions for recommending the use of the term“molecular weight cut off”[J]. Membrane Science and Technology,2006,26(2):82-83.
[8] HY/T 050—1999中空纤维超滤膜测试方法[S].
[9] ASTM E1343—1990(2001)平板超滤膜的分子量界限评定的测试方法[S].
[10] JIS K3821—1990超滤组件的纯净水渗透性能测试方法[S].
[11]吴金克,王彬.超滤膜截留性能测定方法的研究[J].化学工程师,2000,(1):3-5.Wu J K,Wang B. Study on the methods to determine the retention ratio of the ultrafiltration membrane[J].Chemical Engineer,2000,(1):3-5.
[12]邵红霞,江敏芳,赵新建.气相色谱法测定溶剂型涂料中甲苯含量的不确定度评定[J].计量学报,2010,31(1):82-85.Shao H X,Jiang M F,Zhao X J. Uncertainty Evaluation of Toluene in Solvent Based Coatings by GC[J]. Acta Metrologica Sinica,2010,31(1):82-85.
[13] GB/T 32360—2015超滤膜测试方法[S].
[14] JJF 1059—2012测量不确定度评定与表示[S].
[15]马康,李小佳,崔萌萌,等.高效液相色谱法测定葡萄酒中苋菜红的不确定度评定[J].计量学报,2015,36(1):102-106.Ma K,Li X J,Cui M M,et al. Uncertainty Evaluation for the Determination of Amaranthus Red in Wine Samples by High Performance Liquid Chromatography[J]. Acta Metrologica Sinica,2015,36(1):102-106.
[16]郭春刚,潘献辉,李浩,等.新型PDMAA基准物用于超滤膜截留性能检测的研究[J].膜科学与技术,2014,34(2):60-64.Guo C G,Pan X H,Li H,et al. Study on determining of retention performance for ultrafiltration membrane with novel standard substance PDMAA[J]. Membrane Science and Technology,2014,34(2):60-64.
[17] Arkhangelsky E,Duek A,Gitis V. Maximal pore size in UF membranes[J]. Journal of Membrane Science,2012,394-395:89-97.
[18]张激光.分光光度法测定地质样品中MO的不确定度评定[J].计量学报,2013,34(2):196-200.Zhang J G. Uncertainty Evaluation for the Determination of Mo in Geological Samples by Spectrophotometry[J].Acta Metrologica Sinica,2013,34(2):196-200.
[19]罗嫣,张晓慧,石超英.检测条件对中空纤维超滤膜截留率的影响研究[J].广州化工,2015,43(14):123-124,133.Luo Y,Zhang X H,Shi C Y. Analysis of the Influence of Test Conditions on the Rejection of Hollow Fiber Ultrafiltration Membranes[J]. Guang Zhou Chemical Industry and Technology,2015,43(14):123-124,133.
[20]王旭亮,李宗雨,张艳萍,等.聚乙二醇法测定超滤膜截留率的不确定度评[J].计量学报,2018,39(1):119-124.Wang X L,Li Z Y,Zhang Y P,et al. Uncertainty Evaluation for the Determination of the Retention Ratio of the Ultrafiltration Membrane by PEG-20000[J].Acta Metrologica Sinica,2018,39(1):119-124.