紫小麦麸皮花色苷提取及应用研究
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摘要
以紫粒小麦麸皮为原料,采用改良CTAB法提取花色苷,并研究花色苷的体外抗氧化活性和染毛特性。结果表明,改良CTAB提取法易于操作,提取出的花色苷纯度高,室温条件下,浸提3次、提取时间60 min、料液比为1:3时,花色苷的得率为3.20 g/kg。pH值示差法测定提取的花色苷色价为170;原子吸收法测定提取出的花色苷杂质和重金属总含量很低。体外抗氧化试验结果表明,花色苷去除H_2O_2的IC_(50)的大小为1.52,具有较强的抗氧化能力;花色苷和不同金属离子作用后可将羊毛染成不同的颜色。研究结果表明,改良CTAB法提取出的小麦花色苷纯度高,具有较大的开发潜力和实用价值。
The aim is to provide an efficient method for extracting anthocyanins from purple wheat bran using CTAB experiments, the antioxidant activities and wool dyeing of anthocyanins extracted from purple wheat bran were detected. The results indicated that the best parameters of extraction were solid-liquid ratio 1:3,extraction time 60 min and 3 extraction times, and the yield was 3.20 g/kg. UV absorption spectroscopy and atomic absorption spectrometry showed that anthocyanins from purple wheat bran had high color value(170)and low heavy metal content. Antioxidant experiments showed that the anthocyanins from purple wheat bran had strong scavenging capabilities for H_2O_2, with the value of IC_(50) as 1.52. Moreover, the anthocyanins were successfully applied to wool dyeing. In conclusion, the method has high potential for anthocyanins extraction from purple wheat bran.
The aim is to provide an efficient method for extracting anthocyanins from purple wheat bran using CTAB experiments, the antioxidant activities and wool dyeing of anthocyanins extracted from purple wheat bran were detected. The results indicated that the best parameters of extraction were solid-liquid ratio 1:3,extraction time 60 min and 3 extraction times, and the yield was 3.20 g/kg. UV absorption spectroscopy and atomic absorption spectrometry showed that anthocyanins from purple wheat bran had high color value(170)and low heavy metal content. Antioxidant experiments showed that the anthocyanins from purple wheat bran had strong scavenging capabilities for H_2O_2, with the value of IC_(50) as 1.52. Moreover, the anthocyanins were successfully applied to wool dyeing. In conclusion, the method has high potential for anthocyanins extraction from purple wheat bran.
引文
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[23]Sánchez H D,Anderson G H,Poon A N,et al.Maternal fat-soluble vitamins,brain development and regulation of feeding behavior an overview of research[J].Nutrition Research,2016,36(10):1045.
[24]Yang Z D,Zhai W W.Optimization of microwave-assisted extraction of anthocyanins from purple corn(Zea mays L)cob and identification with HPLC-MS[J].Innovative Food Science and Emerging Technologies,2010,11:470.
[25]石荣莹,刘志兵,张蕾,等.天然植物染发剂[J].上海化工,2005,30(6):25-29.
[2]Pascual-teresal S D,Sanchez-ballesta M T.Anthocyaninsfrom plant to health[J].Phytochemistry Reviews,2008,7:281.
[3]Kirakosyan A,Seymour E M,Urcuyollanes D E,et al.Chemical profile and antioxidant capacities of tart cherry products[J].Food Chemistry,2009,115(1):20.
[4]孙建霞,张燕,胡小松,等.花色苷的结构稳定性与降解机制研究进展[J].中国农业科学,2009,42(3):996.
[5]高梓淳,吴涛,陈卫,等.蓝莓花色苷提取与纯化工艺的研究[J].食品与发酵科技,2013,49(3):1.
[6]程秀玮,魏玮,孙勇民.响应面法优化桑葚花色苷提取工艺的研究[J].食品研究与开发,2014,35(14):43.
[7]管敬喜,杨莹,张劲,等.野生毛葡萄皮渣花色苷提取工艺研究[J].酿酒科技,2016,8:40.
[8]贾娜,孔保华,张洪涛,等.黑加仑花色苷的提取及抗氧化活性研究[J].食品科学,2011,32(16):162.
[9]蒋彦婕,吴纪中,张巧凤,等.紫小麦麸皮花色苷提取工艺及其结构[J].江苏农业学报,2012,28(5):1146.
[10]白云凤,李文德,孙善澄,等.黑粒小麦76号的营养品质及其几个理化特性[J].中国粮油学报,2000,15(2):6.
[11]傅虹飞,刘雪梅,孙婧茹,等.紫粒小麦麸皮花色苷提取工艺优化及其抗氧化活性研究[J].食品工业科技,2016,37(1):278.
[12]李伟,唐晓珍,姜媛,等.黑粒小麦麸皮中花色苷的提取及性质研究[J].中国粮油学报,2011,26(10):12.
[13]郑军,谢福来,李世平,等.一种紫小麦麦麸花色苷提取方法[P].中国专利,201510396246.X.
[14]张立伟,刘金,杨频.中草药连翘提取物抗氧化活性研究[J].食品科学,2003,24(2):122.
[15]刘慧芳,张名位,池建伟,等.黑色食品新资源河东乌麦营养成分的评价[J].中国粮油学报,1999,14(2):1.
[16]Odriozola-serrano I,Soliva-fortuny R,Martín-belloso O.Impact of high-intensity pulsed electric fields variables on vitamin C,anthocyanins and antioxidant capacity of strawberry juice[J].LWT-Food Science and Technology,2009,42(1):93.
[17]时玉晴,苏东民,苏东海,等.超声波辅助法提取黑色小麦麸皮花色苷工艺优化[J].粮食与油脂,2015,28(4):15.
[18]杨艳伟,刘思然,张卓娜.染发类产品中染发剂使用现状调查[J].中国卫生检验杂志,2017,3(6):899.
[19]周盟.我国染发剂市场现状[J].日用化学品科学,2008,9(9):6.
[20]汪茹,侯秀良,高海燕,等.膜分离后的儿茶色素染发性能研究[J].天然产物研究与开发,2011,32(1):131.
[21]刘明仁,杨学雨,张昭良.气相色谱质谱-内标法检测染发剂中苯二胺[J].环境化学,2010,12(1):139.
[22]吴光斌,胡阳,倪辉,等.高效液相色谱法同时测定染发剂中10种苯二胺衍生物[J].分析科学学报,2015,28(3):431.
[23]Sánchez H D,Anderson G H,Poon A N,et al.Maternal fat-soluble vitamins,brain development and regulation of feeding behavior an overview of research[J].Nutrition Research,2016,36(10):1045.
[24]Yang Z D,Zhai W W.Optimization of microwave-assisted extraction of anthocyanins from purple corn(Zea mays L)cob and identification with HPLC-MS[J].Innovative Food Science and Emerging Technologies,2010,11:470.
[25]石荣莹,刘志兵,张蕾,等.天然植物染发剂[J].上海化工,2005,30(6):25-29.