食用菌提取多糖对酒精性肝损伤改善测试
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摘要
近些年对于食用菌抑制血糖和抗氧化等研究很多,结果显示其在医药领域有着很好的应用前景。提取出食用菌中的多糖,并观察其对酒精性肝损伤保护作用。将姬菇作为测试食用菌,经抽滤、干燥和纯化提取出姬菇中的多糖当作实验材料。选取雄性6周龄,体重大约为20 g的小白鼠作为实验对象,将实验鼠随机分成对照组和模型组及多糖组。用蒸馏水和实验样品配制,每天对测试对象灌胃一次,持续30 d。31 d时,每组实验鼠禁饮水和吃食12 h后,模型组用50%的乙醇对实验鼠进行灌胃,构建实验鼠酒精性肝损伤模型。取实验鼠肝经过处理放到冰水中超声破碎并制成10%的肝匀浆用于指标测定,将实验状态观测与相关指标测定结果通过SPSS17.0进行处理及分析。实验结果表明,食用菌多糖对实验鼠体重影响不大,对照组和多糖组无死亡,模型组实验鼠存在死亡情况,死亡时间大概集中在1h～2h,食用菌多糖对酒精性肝损伤保护作用十分明显。
In recent years, there have been many studies on the inhibition of blood sugar and antioxidants by Hypsizigus marmoueus, and the results show that it has a good application prospect in the field of medicine. The polysaccharide in Hypsizigus marmoueus was extracted and its protective effect on alcoholic liver injury was observed. Hypsizigus marmoueus was used as the test edible fungus, and the polysaccharide in Hypsizigus marmoueus was extracted by suction filtration, drying and purification as an experimental material. Male 6-week-old mice weight approximately 20 g were selected as experimental subjects, and the rats were randomly divided into control group, model group and polysaccharide group. Prepare with distilled water and experimental samples, and test the subjects once a day for 30 days. At 31 days, each group of rats was banned from drinking water and eating for 12 hours. The model group was intragastrically administrated with 50% ethanol to construct a model of alcoholic liver injury in experimental rats. The liver of the experimental mouse was treated by ultrasonication in ice water and made into10% liver homogenate for index determination. The experimental state observation and related index measurement results were processed and analyzed by SPSS 17.0. The experimental results showed that the edible fungi had little effect on the body weight of the experimental rats, and there was no death in the control group and the polysaccharide group. The death of the model group rats was about 1-2 hours. The protective effect of Hypsizigus marmoueus on alcoholic liver injury was very obvious.
In recent years, there have been many studies on the inhibition of blood sugar and antioxidants by Hypsizigus marmoueus, and the results show that it has a good application prospect in the field of medicine. The polysaccharide in Hypsizigus marmoueus was extracted and its protective effect on alcoholic liver injury was observed. Hypsizigus marmoueus was used as the test edible fungus, and the polysaccharide in Hypsizigus marmoueus was extracted by suction filtration, drying and purification as an experimental material. Male 6-week-old mice weight approximately 20 g were selected as experimental subjects, and the rats were randomly divided into control group, model group and polysaccharide group. Prepare with distilled water and experimental samples, and test the subjects once a day for 30 days. At 31 days, each group of rats was banned from drinking water and eating for 12 hours. The model group was intragastrically administrated with 50% ethanol to construct a model of alcoholic liver injury in experimental rats. The liver of the experimental mouse was treated by ultrasonication in ice water and made into10% liver homogenate for index determination. The experimental state observation and related index measurement results were processed and analyzed by SPSS 17.0. The experimental results showed that the edible fungi had little effect on the body weight of the experimental rats, and there was no death in the control group and the polysaccharide group. The death of the model group rats was about 1-2 hours. The protective effect of Hypsizigus marmoueus on alcoholic liver injury was very obvious.
引文
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[10]谢跃杰,刘葳,张忠明,等.油橄榄叶醇提取物对D-半乳糖胺/脂多糖致小鼠急性肝损伤的保护作用及其作用机制[J].食品工业科技,2018, 39(3):315-319.
[2]黄小莉,朱培欣,胡远亮,等.蛹虫草多糖对急性酒精性肝损伤改善作用的研究[J].菌物学报,2017, 36(2):242-250.
[3]毕博,于荣利.尖顶羊肚菌多糖的超声波辅助提取工艺优化及其对肝损伤小鼠抗氧化活性的影响[J].食用菌学报,2016, 23(3):53-56.
[4]赵爽,荣成博,刘宇,等.3种食用菌分级多糖消脂护肝功能研究[J].食品研究与开发,2017, 38(8):168-172.
[5]高月求,刘耀文,单俊杰.天然多糖对实验免疫性和酒精性肝损伤的干预评价研究进展[J].国际药学研究杂志,2017, 44(11):1075-1084.
[6]杜娟,时文静.香菇、金针菇、黑木耳多糖的提取与测定[J].江苏农业科学,2016, 44(8):347-350.
[7]徐博,沈楠,安英,等.汉防己多糖对急性酒精性肝损伤小鼠氧化应激及肝细胞凋亡的影响[J].中国药房,2017,28(7):885-888.
[8]叶文斌,樊亮,王昱,等.黄果槲寄生果实多糖对急性酒精肝损伤小鼠的保护作用[J].安徽农业大学学报,2017,44(2):218-223.
[9]张强,林立青,董祺,等.姬松茸液体发酵培养基优化及其对小鼠酒精性肝损伤保护作用[J].中国酿造,2016,35(12):76-79.
[10]谢跃杰,刘葳,张忠明,等.油橄榄叶醇提取物对D-半乳糖胺/脂多糖致小鼠急性肝损伤的保护作用及其作用机制[J].食品工业科技,2018, 39(3):315-319.