不同盐胁迫条件下东方杉的生长及生理响应研究
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摘要
以健壮且长势一致的2年生东方杉为试验材料,通过设置不同浓度盐胁迫处理,采用水培的方法,分析东方杉的存苗率、枝条增量、叶绿素含量、丙二醛含量、相对电导率及超氧化物歧化酶活性的变化,研究东方杉的抗盐水平及其在不同程度盐胁迫条件下的生长生理变化。结果表明:3‰NaCl胁迫前40 d,东方杉的生长生理变化与CK差异不显著,在第50天时,3‰NaCl胁迫的东方杉生长略微下降。而东方杉在6‰NaCl胁迫下,东方杉生长明显受到抑制,存苗率显著低于CK和3‰NaCl处理;叶片中叶绿素含量明显下降,丙二醛含量和相对电导率显著高于CK,细胞膜完整性遭到一定程度的破坏。可见,东方杉可在短期内抵抗低盐浓度3‰NaCl胁迫,但无法消除6‰盐胁迫引起的生长抑制。
Taking strong and consistent 2-year-old Taxodium mucronatum×Cryptomeria fortunei as material.By setting different concentrations of salt stress treatment, hydroponic method was used to analyze the seedling rate, shoot increment, chlorophyll content, malondialdehyde content, relative conductivity and superoxide dismutase. The salt tolerance and physiological changes under different degrees of salt stress were studied. Results show that there is no significant difference between CK and the 3‰ NaCl stress on the growth and physiology changes under 40 days treatment. At the 50 th day of the experiment, the growth of T. mucronatum×C. fortunei treated with 3‰ NaCl is slightly decreased. However, under the 6‰ NaCl stress, the growth of T. mucronatum×C.fortunei is significantly inhibited, and the seedling survival rate is significantly lower than that of CK and 3‰NaCl stress. And chlorophyll content in leaves decreases significantly, MDA content and relative conductivity are significantly higher than those of CK, and the integrity of the cell membrane is destroyed to a certain extent. Thus,T. mucronatum×C. fortunei can resist low salt concentration 3‰ NaCl stress in a short period of time, but it can not eliminate the growth inhibition caused by 6‰ salt stress.
Taking strong and consistent 2-year-old Taxodium mucronatum×Cryptomeria fortunei as material.By setting different concentrations of salt stress treatment, hydroponic method was used to analyze the seedling rate, shoot increment, chlorophyll content, malondialdehyde content, relative conductivity and superoxide dismutase. The salt tolerance and physiological changes under different degrees of salt stress were studied. Results show that there is no significant difference between CK and the 3‰ NaCl stress on the growth and physiology changes under 40 days treatment. At the 50 th day of the experiment, the growth of T. mucronatum×C. fortunei treated with 3‰ NaCl is slightly decreased. However, under the 6‰ NaCl stress, the growth of T. mucronatum×C.fortunei is significantly inhibited, and the seedling survival rate is significantly lower than that of CK and 3‰NaCl stress. And chlorophyll content in leaves decreases significantly, MDA content and relative conductivity are significantly higher than those of CK, and the integrity of the cell membrane is destroyed to a certain extent. Thus,T. mucronatum×C. fortunei can resist low salt concentration 3‰ NaCl stress in a short period of time, but it can not eliminate the growth inhibition caused by 6‰ salt stress.
引文
[1]张建军,潘士华,沈烈英,等.东方杉的树种特征与生态价值[J].上海农业学报,2003,19(3):56-59.
[2]张晓磊,马风云,马玉辉,等.盐胁迫对东方杉生长和生理生化的影响[J].中国农学通报,2010,26(14):145-148.
[3]孙文喜,孙文鑫,周大旺,等.植物抗盐性生理生化研究进展及提高植物抗盐性对策[J].农业科技通讯,2013(6):174-178.
[4]崔世友,夏礼如,邱海荣.植物耐盐性研究进展[J].长江大学学报自然科学版(农学卷),2012,9(10):17-22.
[5]刘娥娥,黎用朝.干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响[J].热带亚热带植物学报,2000,8(3):235-238.
[6]王琪,袁燕波,于晓南.盐碱胁迫下2个芍药品种生理特性及耐盐碱性研究[J].河北农业大学学报,2013,36(6):52-60.
[7]Arnon D I.Copper enzymes in isolated chloroplasts.polyphenoloxidase in Beta vulgaris[J].Plant Physiology,1949,24(1):1-15.
[8]McCord J M,Fridovich I.Superoxide dismutase an enzymic function for erythrocuprein(hemocuprein)[J].Journal of Biological chemistry,1969,244(22):6049-6055.
[9]Buege J,Aust S.On the solubilization of NADPH-cytochrome c reductase from rat liver microsomes with crude pancreatic lipase[J].Biochimica et Biophysica Acta-General Subjects,1972,286(2):433-436.
[10]杨鹏辉,李贵全,郭丽,等.干旱胁迫对不同抗旱大豆品种花荚期质膜透性的影响[J].干旱地区农业研究,2003,21(3):127-130.
[11]Li T,Hu Y,Du X,et al.Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis cv.Merrillii seedlings by activating photosynthesis and enhancing antioxidant systems[J].PLoS One,2014,9(10):1-9.
[12]戴文君,陆海燕,杨梅,等.稀土元素镧、铈对擎天树幼苗生长量的影响[J].西南林业大学学报(自然科学),2018,38(1):1-15.
[13]刘真华,曹灿景.盐胁迫对彩叶草种子萌发.光合特性.叶绿素荧光及无机离子代谢的影响[J].西部林业科学,2018,47(6):78-84.
[14]MunnsR,TemmatA.Whole plant responses to salinity[J].Functional Plant Biology,1986,13(1):143-160.
[15]金雅琴,李冬林,丁雨龙,等.盐胁迫对乌桕幼苗光合特性及叶绿素含量的影响[J].南京林业大学学报(自然科学版),2011,35(1):29-33.
[16]Mansour M M F.Plasma membrane permeability as an indicator of salt tolerance in plants[J].Biologia Plantarum,2012,57(1):1-10.
[17]钱琼秋,朱祝军,何勇.硅对盐胁迫下黄瓜根系线粒体呼吸作用及脂质过氧化的影响[J].植物营养与肥料学报,2016,12(6):875.
[18]Hakeem K R,Khan F,Chandna R,et al.Genotypic variability among soybean genotypes under NaCl stress and proteome analysis of salt-tolerant genotype[J].Applied biochemistry&biotechnology,2012,168(8):2309-2329.
[19]刘海芳,王凡.重金属对水产动物污染的生物标志物的研究进展[J].水产科学,2009,28(5):299-302.
[2]张晓磊,马风云,马玉辉,等.盐胁迫对东方杉生长和生理生化的影响[J].中国农学通报,2010,26(14):145-148.
[3]孙文喜,孙文鑫,周大旺,等.植物抗盐性生理生化研究进展及提高植物抗盐性对策[J].农业科技通讯,2013(6):174-178.
[4]崔世友,夏礼如,邱海荣.植物耐盐性研究进展[J].长江大学学报自然科学版(农学卷),2012,9(10):17-22.
[5]刘娥娥,黎用朝.干旱、盐和低温胁迫对水稻幼苗脯氨酸含量的影响[J].热带亚热带植物学报,2000,8(3):235-238.
[6]王琪,袁燕波,于晓南.盐碱胁迫下2个芍药品种生理特性及耐盐碱性研究[J].河北农业大学学报,2013,36(6):52-60.
[7]Arnon D I.Copper enzymes in isolated chloroplasts.polyphenoloxidase in Beta vulgaris[J].Plant Physiology,1949,24(1):1-15.
[8]McCord J M,Fridovich I.Superoxide dismutase an enzymic function for erythrocuprein(hemocuprein)[J].Journal of Biological chemistry,1969,244(22):6049-6055.
[9]Buege J,Aust S.On the solubilization of NADPH-cytochrome c reductase from rat liver microsomes with crude pancreatic lipase[J].Biochimica et Biophysica Acta-General Subjects,1972,286(2):433-436.
[10]杨鹏辉,李贵全,郭丽,等.干旱胁迫对不同抗旱大豆品种花荚期质膜透性的影响[J].干旱地区农业研究,2003,21(3):127-130.
[11]Li T,Hu Y,Du X,et al.Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis cv.Merrillii seedlings by activating photosynthesis and enhancing antioxidant systems[J].PLoS One,2014,9(10):1-9.
[12]戴文君,陆海燕,杨梅,等.稀土元素镧、铈对擎天树幼苗生长量的影响[J].西南林业大学学报(自然科学),2018,38(1):1-15.
[13]刘真华,曹灿景.盐胁迫对彩叶草种子萌发.光合特性.叶绿素荧光及无机离子代谢的影响[J].西部林业科学,2018,47(6):78-84.
[14]MunnsR,TemmatA.Whole plant responses to salinity[J].Functional Plant Biology,1986,13(1):143-160.
[15]金雅琴,李冬林,丁雨龙,等.盐胁迫对乌桕幼苗光合特性及叶绿素含量的影响[J].南京林业大学学报(自然科学版),2011,35(1):29-33.
[16]Mansour M M F.Plasma membrane permeability as an indicator of salt tolerance in plants[J].Biologia Plantarum,2012,57(1):1-10.
[17]钱琼秋,朱祝军,何勇.硅对盐胁迫下黄瓜根系线粒体呼吸作用及脂质过氧化的影响[J].植物营养与肥料学报,2016,12(6):875.
[18]Hakeem K R,Khan F,Chandna R,et al.Genotypic variability among soybean genotypes under NaCl stress and proteome analysis of salt-tolerant genotype[J].Applied biochemistry&biotechnology,2012,168(8):2309-2329.
[19]刘海芳,王凡.重金属对水产动物污染的生物标志物的研究进展[J].水产科学,2009,28(5):299-302.