偏痛汤1号化学成分的UHPLC-LTQ-OrbitrapMS~n快速鉴定研究
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摘要
建立超高效液相色谱-线性离子阱/静电场轨道阱组合式高分辨质谱联用(UHPLC-LTQ-Orbitrap MS)技术分析偏痛汤1号化学成分的方法。方法:采用UHPLC BEH C18色谱柱(2. 1 mm×50 mm,1. 7μm),以乙腈0. 1%甲酸水为流动相进行梯度洗脱。分别在正、负离子模式下,采集LTQOrbitrap MS数据,依据保留时间信息、高分辨质谱精确分子量和MSn多级碎片信息分析偏痛汤1号全方的化学成分以及方中蔓荆子、甘草和细辛三味中药的特征性成分。结果:从偏痛汤1号中共鉴定出126种化学成分,其中17种化学成分为蔓荆子特有,60种化学成分为炙甘草特有,5种化学成分为细辛特有。结论:该方法可为偏痛汤1号质量控制、药效学研究提供参考数据,为复方中药化学成分的快速定性分析提供借鉴。
引文
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[3]罗亚敏,宋慧荣,杨蕾,等.偏痛汤1号对偏头痛模型大鼠CGRP/PGE2/TNF-α表达的影响[J].中国中西医结合杂志,2018,38(12):1484--1489.
[4]田华,杜婷,黄开合,等.蔓荆子的药理作用研究进展[J].中国医药导报,2013,10(9):29-30.
[5]梁学清,李丹丹.细辛药理作用研究进展[J].河南科技大学学报(医学版),2011,29(4):318-320.
[6]姜雪,孙森凤,王悦,等.甘草药理作用研究进展[J].化工时刊,2017,31(7):25-28.
[7]张永,严安定,高建.液质联用技术鉴定甘草提取物中的主要化学成分[J].中成药,2012,34(6):1111-1115.
[8]Yahara S,Nishioka I. Flavonoid glucosides from licorice[J]. Phytochemistry,1984,23(23):2108-2109.
[9]张航航,葛振红,李国柱,等.甘草甜味素的化学成分研究[J].食品工业科技,2019,40(2):229-232.
[10]Hatano T,Takagi M,Ito H,et al. Acylated flavonoid glycosides and accompanying phenolics from licorice[J]. Phytochemistry,1998,47(2):287-293.
[11]Kobayashi M,Kawazoe K,Okamoto T,et al. Marine Natural Products.XXXI. Structure-Activity Correlation of a Potent Cytotoxic Dimeric Macrolide Swinholide A,from the Okinawan Marine Sponge Theonellaswinhoei,and Its Isomers[J]. Chemical&Pharmaceutical Bulletin,1994,42(1):19-26.
[12]Zhang P C,Sui Xu X U. Two New C-glucoside Flavonoids from Leaves of CrataeguspinnatifidaBge. var. major N. E. Br[J]. Chinese Chemical Letters,2002,13(4):337-340.
[13]IWASHINA,KAMENOSONO,UENO. Hispidulin and nepetin 4'-glucosides from Cirsiumoligophyllum[J]. Phytochemistry,1999,51(8):1109-1111.
[14]Harborne J B,Baxter H. The handbook of natural flavonoids[J]. Handbook of Natural Flavonoids,1999,1:549.
[15]周燕,王明奎,廖循,等.甘草化学成分的高效液相色谱-串联质谱分析[J].分析化学,2004,32(2):174-178.
[16]Southon I W,Bisby F A,Buckingham J,et al. Phytochemical dictionary of the Leguminosae. Volume 1. Plants and their constituents[J]. Chemical constituents. 1994,2.
[17]ShibataM,Nakahara N,Aimi. Paeoniflorin,aglucoside of Chinese Paeony root[J]. Chem. Pharm. Bull,1963,11(3):372-378.