林下参与园参对术后疲劳综合征大鼠的脑保护作用
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摘要
目的:研究林下参和园参对术后疲劳综合征(POFS)大鼠的抗疲劳机制,比较两种人参对术后中枢疲劳的改善作用。方法:将96只雄性SD大鼠,随机分为对照组、POFS模型组(POFS组)、园参干预组和林下参干预组,每组再按时间点分为术后1、3 d组。术后对应时间点进行强迫游泳实验(FST);检测大脑海马丙二醛(MDA)的量和总超氧化物歧化酶(T-SOD)的活性; Western blotting检测Bax和Bcl-2的蛋白表达; HE染色观察海马CA1区神经细胞形态。结果:模型组与对照组比较,术后1、3 d不动时间明显增加(P <0. 01),MDA的量明显增加(P <0. 01),术后3 d T-SOD活性明显升高(P <0. 05),Bax表达显著升高,Bcl-2表达显著降低;园参组与模型组比较,术后1、3 d不动时间减少(P <0. 05),术后3 d T-SOD活性明显升高(P <0. 05),Bax表达降低,Bcl-2表达升高;林下参组与模型组比较,术后1、3 d不动时间明显减少(P <0. 01),MDA的量明显减少(P <0. 05,0. 01),术后3 d T-SOD活性明显升高(P <0. 01),Bax表达降低,Bcl-2表达升高;林下参组与园参组比较,术后1、3 d不动时间减少(P <0. 05,0. 01),术后3 d MDA的量减少(P <0. 05),T-SOD活性明显升高(P <0. 05),术后1、3 d Bax表达水平降低,Bcl-2表达水平升高。HE染色结果显示,模型组与对照组比较,大鼠海马CA1区神经细胞受损,数量降少,人参干预组与模型组比较,神经细胞形态得到改善,数量增多。结论:POFS大鼠神经细胞存在一定程度损伤,园参和林下参改善了POFS大鼠的脑细胞损伤,林下参效果优于园参。
Objective:To study the anti-fatigue mechanisms of Ginseng under Forest( GUF) and Ginseng Cultivated( GC) in rats with Postoperative Fatigue Syndrome( POFS) and compare the effects of two kinds of ginseng on central fatigue. Methods:Ninety-six male Sprague-Dawley rats were randomly divided into control,POFS,Ginseng under Forest( GUF) and Ginseng Cultivated( GC) intervention groups,and each group was divided into postoperative 1,3 d groups according to time points. The fatigue was assessed with forced swimming test( FST). The content of malondialdehyde( MDA) and the activity of total superoxide dismutase( T-SOD) in the hippocampus were detected. The protein expressions of Bax and Bcl-2 were examined by Western blotting. Cell morphology of hippocampal CA1 area was observed through HE staining. Results:Compared with the control group,the immobility time in the POFS group was significantly increased on postoperative day 1 and day 3( P < 0. 01) and the content of MDA was significantly increased( P < 0. 01) and the activity of T-SOD was significantly increased on postoperative day 3( P <0. 05). The expression of Bax protein increased significantly,and the expression of Bcl-2 protein decreased significantly. Compared with the POFS group,the immobility time in the GC group was significantly increased on postoperative day 1 and day 3( P <0. 05). The activity of T-SOD significantly increased on postoperative day 3( P < 0. 05). The expression of Bax protein decreased and the expression of Bcl-2 protein increased. Compared with the POFS group,the immobility time in the GUF group was significantly decreased on postoperative day 1 and day 3( P < 0. 01). The content of MDA was significantly decreased( P < 0. 05,P <0. 01),and the activity of T-SOD increased significantly( P < 0. 01). The expression of Bax protein decreased and the expression of Bcl-2 protein increased on postoperative day 1 and day 3. Compared with the GC group,the immobility time in the GUF group decreased on postoperative day 1 and day 3( P < 0. 05,P < 0. 01). The content of MDA decreased( P < 0. 05) and the activity of T-SOD significantly increased( P < 0. 05) on postoperative day 3. On postoperative day 1 and day 3,the expression of Bax protein decreased and the expression of Bcl-2 protein increased. Compared with the control group,the results of HE staining showed that the nerve cells in the CA1 region of the hippocampus of the model group were damaged and the number was reduced. Compared with the model group,the morphology of the nerve cells was improved in the ginseng intervention group. Conclusion:The nerve cells of POFS rats were damaged to a certain extent. Ginseng under Forest and Ginseng Cultivated improved the brain cell of POFS rats. The effect of Ginseng under Forest was better than that of Ginseng Cultivated.
Objective:To study the anti-fatigue mechanisms of Ginseng under Forest( GUF) and Ginseng Cultivated( GC) in rats with Postoperative Fatigue Syndrome( POFS) and compare the effects of two kinds of ginseng on central fatigue. Methods:Ninety-six male Sprague-Dawley rats were randomly divided into control,POFS,Ginseng under Forest( GUF) and Ginseng Cultivated( GC) intervention groups,and each group was divided into postoperative 1,3 d groups according to time points. The fatigue was assessed with forced swimming test( FST). The content of malondialdehyde( MDA) and the activity of total superoxide dismutase( T-SOD) in the hippocampus were detected. The protein expressions of Bax and Bcl-2 were examined by Western blotting. Cell morphology of hippocampal CA1 area was observed through HE staining. Results:Compared with the control group,the immobility time in the POFS group was significantly increased on postoperative day 1 and day 3( P < 0. 01) and the content of MDA was significantly increased( P < 0. 01) and the activity of T-SOD was significantly increased on postoperative day 3( P <0. 05). The expression of Bax protein increased significantly,and the expression of Bcl-2 protein decreased significantly. Compared with the POFS group,the immobility time in the GC group was significantly increased on postoperative day 1 and day 3( P <0. 05). The activity of T-SOD significantly increased on postoperative day 3( P < 0. 05). The expression of Bax protein decreased and the expression of Bcl-2 protein increased. Compared with the POFS group,the immobility time in the GUF group was significantly decreased on postoperative day 1 and day 3( P < 0. 01). The content of MDA was significantly decreased( P < 0. 05,P <0. 01),and the activity of T-SOD increased significantly( P < 0. 01). The expression of Bax protein decreased and the expression of Bcl-2 protein increased on postoperative day 1 and day 3. Compared with the GC group,the immobility time in the GUF group decreased on postoperative day 1 and day 3( P < 0. 05,P < 0. 01). The content of MDA decreased( P < 0. 05) and the activity of T-SOD significantly increased( P < 0. 05) on postoperative day 3. On postoperative day 1 and day 3,the expression of Bax protein decreased and the expression of Bcl-2 protein increased. Compared with the control group,the results of HE staining showed that the nerve cells in the CA1 region of the hippocampus of the model group were damaged and the number was reduced. Compared with the model group,the morphology of the nerve cells was improved in the ginseng intervention group. Conclusion:The nerve cells of POFS rats were damaged to a certain extent. Ginseng under Forest and Ginseng Cultivated improved the brain cell of POFS rats. The effect of Ginseng under Forest was better than that of Ginseng Cultivated.
引文
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[11] Rosa A P,Mescka C P,Catarino F M,et al. Neonatal hyperglycemia induces cell death in the rat brain[J]. Metabolic Brain Disease,2017(3):1-10.
[12] Shergis J L,Zhang A L,Zhou W,et al. Panax ginseng in randomised controlled trials:a systematic review[J]. Phytotherapy Research,2013,27(7):949-965.
[2]何绍玉,李伟,郑毅男,等.林下山参与园参中腺苷和人参皂苷含量比较分析[J].药物分析杂志,2010(9):1701-1706.
[3] Oliveira M,Oliveira G,Souza-Talarico J,et al. Surgical Oncology:Evolution of Postoperative Fatigue and Factors Related to Its Severity[J]. Clinical Journal of Oncology Nursing,2016,20(1):E3-E8.
[4] Zargar-Shoshtari KHill A G. Postoperative Fatigue:A Review[J].World Journal of Surgery,2009,33(4):738.
[5] Zhang X D,Chen B C,Dong Q T,et al. Establishment and assessments of a new model for the postoperative fatigue syndrome by major small intestinal resection in rats[J]. Scandinavian Journal of Gastroenterology,2011,46(11):1302.
[6]刘舒,吕金晓,郑蓓诗,等.人参皂苷Rb_1改善术后疲劳综合征大鼠中枢炎症反应的机制研究[J].中草药,2015,46(14):2104-2110.
[7] Kantarci K. Fractional anisotropy of the fornix and hippocampal atrophy in Alzheimer's disease[J]. Frontiers in Aging Neuroscience,2014,6(6):316.
[8] Borsini FMeli A. Is the forced swimming test a suitable model for revealing antidepressant activity?[J]. Psychopharmacology,1988,94(2):147-160.
[9] Rosenfeldt,Franklin,Wilson,et al. Oxidative stress in surgery in an ageing population:Pathophysiology and therapy[J]. Experimental Gerontology,2013,48(1):45-54.
[10] Atalay T,Gulsen I,Colcimen N,et al. Resveratrol treatment prevents hippocamal neurodegeneration in a rodent model of traumatic brain injury[J]. Turkish Neurosurgery,2017,27(6):924-930.
[11] Rosa A P,Mescka C P,Catarino F M,et al. Neonatal hyperglycemia induces cell death in the rat brain[J]. Metabolic Brain Disease,2017(3):1-10.
[12] Shergis J L,Zhang A L,Zhou W,et al. Panax ginseng in randomised controlled trials:a systematic review[J]. Phytotherapy Research,2013,27(7):949-965.