杂草稻抗白叶枯病基因的筛选与鉴定
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摘要
为进一步发掘杂草稻的抗白叶枯病基因,同时为水稻白叶枯病抗病育种提供材料基础及理论参考,通过接种鉴定与分子标记检测分析,对148份杂草稻中的抗白叶枯病基因进行发掘和鉴定。结果表明:在148份杂草稻中获得1份中抗白叶枯病的材料W132,分子标记检测发现在W132中不含Xa23和Xa21基因,含有Xa7基因。该材料可作为水稻抗白叶枯病抗病育种的基础种质加以利用。
In order to further explore the resistance to bacterial blight of weedy rice,and meanwhile provide material basis and theoretical reference for rice bacterial blight resistance breeding,through inoculation identification and molecular marker detection analysis,genes resistant to bacterial blight of 148 weedy rice cultivars were explored and identified.Results:A medium resistant material W132 was obtained in the 148 weedy rice cultivars,the resistance gene Xa7 gene was identified in W132,but Xa23 and Xa21 were not found.This material can be used as a basic germplasm for rice resistance to bacterial blight resistance breeding.
In order to further explore the resistance to bacterial blight of weedy rice,and meanwhile provide material basis and theoretical reference for rice bacterial blight resistance breeding,through inoculation identification and molecular marker detection analysis,genes resistant to bacterial blight of 148 weedy rice cultivars were explored and identified.Results:A medium resistant material W132 was obtained in the 148 weedy rice cultivars,the resistance gene Xa7 gene was identified in W132,but Xa23 and Xa21 were not found.This material can be used as a basic germplasm for rice resistance to bacterial blight resistance breeding.
引文
[1]王黎明,陈勇.杂草稻研究现状及利用展望[J].植物保护,2009,35(5):14-17.
[2]WANG FENG,YUAN QIANHUA,SHI LEI,et al.Alarge-scale field study of transgene flow from cultivated rice(Oryza sativa)to common wild rice(O.rufipogon)and barnyard grass(Echi-nochloa crusg alli)[J].Plant Biotechnology Journal,2006(4):667-676.
[3]郭俊祥,邹德堂,孙世臣,等.黑龙江省典型杂草稻杂交后代野生性状的比较分析[J].黑龙江农业科学,2006(6):11-13.
[4]张忠林,谭学林,邓安凤.杂草稻种质资源的综合评价[J].植物遗传资源学,2002,3(4):47-50.
[5]王丽丽,赵家铭,马作斌,等.辽宁地区水稻资源抗稻瘟病基因的检测分析[J].植物遗传资源学报,2017,18(2):325-339.
[6]覃宝祥,刘驰,焦晓真,等.广西普通野生稻白叶枯病广谱抗源的鉴定与评价[J].南方农业学报,2014,45(9):1527-1531.
[7]MURRAY M G,THOMPSON W F.Rapid isolation of high molecular weight plant DNA[J].Nucl Acids Res,1980,8:4321-4325.
[8]朱玉君,樊叶杨,王惠梅,等.应用分子标记辅助选择培育兼抗稻瘟病和白叶枯病的水稻恢复系[J].分子植物育种,2014,12(1):17-24.
[9]高利军,高汉亮,李容柏,等.水稻抗白叶枯病基因Xa23分子标记的优化和验证[J].分子植物育种,2010,8(4):660-664.
[10]闫成业,刘艳,牟同敏.分子标记辅助选择聚合Xa7、Xa21和cry1C*基因改良杂交水稻金优207的白叶枯病和螟虫抗性[J].杂交水稻,2013,28(5):52-59.
[11]袁晓丹,王亮,王丽丽,等.东北地区杂草稻的抗逆性及遗传特性[J].延边大学农学学报,2006,28(4):233-238.
[12]SUH H S,SATO Y I,MORISHIMA H.Genetic characterization of weedy rice(Oryza sativa L.)based on morpho-physiology,isozymes and RAPD markers[J].Theoretical and Applied Genetics,1997,94:316-321.
[13]曹卿,张玉琼,董召荣,等.利用显性标记ST10对96份江苏省杂草稻基因型的分析[J].分子植物育种,2011,9(1):87-90.
[2]WANG FENG,YUAN QIANHUA,SHI LEI,et al.Alarge-scale field study of transgene flow from cultivated rice(Oryza sativa)to common wild rice(O.rufipogon)and barnyard grass(Echi-nochloa crusg alli)[J].Plant Biotechnology Journal,2006(4):667-676.
[3]郭俊祥,邹德堂,孙世臣,等.黑龙江省典型杂草稻杂交后代野生性状的比较分析[J].黑龙江农业科学,2006(6):11-13.
[4]张忠林,谭学林,邓安凤.杂草稻种质资源的综合评价[J].植物遗传资源学,2002,3(4):47-50.
[5]王丽丽,赵家铭,马作斌,等.辽宁地区水稻资源抗稻瘟病基因的检测分析[J].植物遗传资源学报,2017,18(2):325-339.
[6]覃宝祥,刘驰,焦晓真,等.广西普通野生稻白叶枯病广谱抗源的鉴定与评价[J].南方农业学报,2014,45(9):1527-1531.
[7]MURRAY M G,THOMPSON W F.Rapid isolation of high molecular weight plant DNA[J].Nucl Acids Res,1980,8:4321-4325.
[8]朱玉君,樊叶杨,王惠梅,等.应用分子标记辅助选择培育兼抗稻瘟病和白叶枯病的水稻恢复系[J].分子植物育种,2014,12(1):17-24.
[9]高利军,高汉亮,李容柏,等.水稻抗白叶枯病基因Xa23分子标记的优化和验证[J].分子植物育种,2010,8(4):660-664.
[10]闫成业,刘艳,牟同敏.分子标记辅助选择聚合Xa7、Xa21和cry1C*基因改良杂交水稻金优207的白叶枯病和螟虫抗性[J].杂交水稻,2013,28(5):52-59.
[11]袁晓丹,王亮,王丽丽,等.东北地区杂草稻的抗逆性及遗传特性[J].延边大学农学学报,2006,28(4):233-238.
[12]SUH H S,SATO Y I,MORISHIMA H.Genetic characterization of weedy rice(Oryza sativa L.)based on morpho-physiology,isozymes and RAPD markers[J].Theoretical and Applied Genetics,1997,94:316-321.
[13]曹卿,张玉琼,董召荣,等.利用显性标记ST10对96份江苏省杂草稻基因型的分析[J].分子植物育种,2011,9(1):87-90.