吴茱萸碱与脂质体模拟生物膜的相互作用: FTIR和DSC研究
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摘要
药物与生物膜相互作用的研究对于了解药物药效和改善其生物性能具有重要的意义。但生物膜的组成复杂,直接研究药物活性成分与生物膜的相互作用比较困难。以脂质体作为生物膜模型,研究了吴茱萸碱与脂质体的相互作用,分析了吴茱萸碱分子在脂质体中的包封位置,探讨了吴茱萸碱抗炎作用可能的作用机制。以二棕榈酰磷脂酰胆碱(DPPC)为膜材,应用薄膜分散法制备含有不同摩尔百分比(x)的吴茱萸碱脂质体,应用傅立叶变换红外光谱(FTIR)和差示扫描量热(DSC)技术分析随着脂质体中药物摩尔百分比的增大, DPPC分子各红外特征吸收峰频率、峰形及量热参数的变化情况,从而探讨药物在脂质体中的包封位置及吴茱萸碱分子对脂质体膜流动性的影响。实验数据表明,在0 The study of the interaction between drugs and biomembrane is of great significance to the understanding of the drug efficacy and the improvment of their biological properties. However, the composition of biomembrane is complex, which makes it difficult to study the interaction between active components of drugs and biomembrane directly. We used liposome as mimetic biomembrane, investigated the interaction of evodiamine with liposome, analyzed the entrapment position of evodiamine among liposome, and the possible mechanism of the anti-inflammatory effect of evodiamine was also discussed. 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine(DPPC) has been used as membrane material in this study and liposomes containing different molar percentage of evodiamine(x) were prepared by thin-film dispersion method. Fourier transform infrared spectroscopy(FTIR) and Differential scanning calorimetry(DSC) were used to analyze the frequency and shape of infrared absorption peaks and the changes of the calorimetric parameters of DPPC molecules with the increasing of the molar percentage of drugs. The entrapment position of the evodiamine in liposome and the effect of this drug on the fluidity of liposome membrane were discussed. Data showed that the frequency of the asymmetric stretching vibration of the phosphate group in the DPPC head region hardly changed in the concentration range of 0
引文
[1] Chinese Pharmacopoeia Commission(中华人民共和国药典委员会).Pharmacopoeia of the People’s Republic of China,Part One(中华人民共和国药典一部).Beijing:China Medical Science Press(北京:中国医药科技出版社),2015.
[2] ZHANG Zhi-xian,JIANG Mei-ling,WANG Xin-hui,et al(张志仙,蒋美玲,王欣慧,等).Progress in Modern Biomedicine(现代生物医学进展),2014,14(21):4189.
[3] Sugimoto T,Miyase T,Kuroyanagi M,et al.Chemical and Pharmaceutical Bulletin,2011,36(11):4453.
[4] Huang X,Li W,Yang X W.Fitoterapia,2012,83(4):709.
[5] LU Shan-shan,JIANG Xiao-yan,GE Miao,et al(卢杉杉,姜晓燕,葛苗,等).Pharmaceutical Biotechnology(药物生物技术),2014,(3):231.
[6] Nunes C,Brezesinski G,Lima J L,et al.Journal of Physical Chemistry B,2011,115(24):8024.
[7] Wei T T,Sun H Y,Deng G,et al.Journal of Thermal Analysis and Calorimetry,2018,132(1):685.
[8] Gmajner D,Ulrih N P.Journal of Thermal Analysis and Calorimetry,2011,106(1):255.
[9] Bakonyi M,Berkó S,Budai-Szücs M,et al.Journal of Thermal Analysis and Calorimetry,2017,130(7):1.
[10] Wu F G,Sun H Y,Zhou Y,et al.Rsc Advances,2014,4(93):51171.
[11] Manrique-Moreno M,Heinbockel L,Suwalsky M,et al.Biochimica Biophysica Acta-Biomembranes,2016,1858(9):2123.
[12] L?rincz A,Mihály J,Németh C,et al.Biochimica Biophysica Acta-Biomembranes,2015,1848(5):1092.
[13] Berényi S,Mihály J,Wacha A,et al.Colloids Surf B Biointerfaces,2014,118(22):164.
[14] Kamiński D M,Arczewska M,Pociecha D,et al.Journal of Molecular Structure,2015,1080:57.
[15] Wu F G,Jia Q,Wu R G,et al.Journal of Physical Chemistry B,2011,115(26):8559.
[16] Wu F G,Wang N N,Yu J S,et al.Journal of Physical Chemistry B,2010,114(6):2158.
[17] Bilge D,Kazanci N,Severcan F.Journal of Molecular Structure,2013,1040(20):75.
[18] Wu R G,Wang Y R,Wu F G,et al.Journal of Thermal Analysis and Calorimetry,2012,109(1):311.
[19] Berényi S,Mihály J,Kristyán S,et al.Biochimica Et Biophysica Acta-Biomembranes,2013,1828(2):661.
[20] Wu R G,Dai J D,Wu F G,et al.Int.J.Pharm.,2012,438(1-2):91.
[21] Pili B,Bourgaux C,Amenitsch H,et al.Biochimica Biophysica Acta-Biomembranes,2010,1798(8):1522.
[22] Kalamkar V,Joshi M,Borkar V,et al.Bioorganic and Medicinal Chemistry,2013,21(21):6753.
[23] Nunes C,Brezesinski G,Lima J L F C,et al.Soft Matter,2011,7(6):3002.
[2] ZHANG Zhi-xian,JIANG Mei-ling,WANG Xin-hui,et al(张志仙,蒋美玲,王欣慧,等).Progress in Modern Biomedicine(现代生物医学进展),2014,14(21):4189.
[3] Sugimoto T,Miyase T,Kuroyanagi M,et al.Chemical and Pharmaceutical Bulletin,2011,36(11):4453.
[4] Huang X,Li W,Yang X W.Fitoterapia,2012,83(4):709.
[5] LU Shan-shan,JIANG Xiao-yan,GE Miao,et al(卢杉杉,姜晓燕,葛苗,等).Pharmaceutical Biotechnology(药物生物技术),2014,(3):231.
[6] Nunes C,Brezesinski G,Lima J L,et al.Journal of Physical Chemistry B,2011,115(24):8024.
[7] Wei T T,Sun H Y,Deng G,et al.Journal of Thermal Analysis and Calorimetry,2018,132(1):685.
[8] Gmajner D,Ulrih N P.Journal of Thermal Analysis and Calorimetry,2011,106(1):255.
[9] Bakonyi M,Berkó S,Budai-Szücs M,et al.Journal of Thermal Analysis and Calorimetry,2017,130(7):1.
[10] Wu F G,Sun H Y,Zhou Y,et al.Rsc Advances,2014,4(93):51171.
[11] Manrique-Moreno M,Heinbockel L,Suwalsky M,et al.Biochimica Biophysica Acta-Biomembranes,2016,1858(9):2123.
[12] L?rincz A,Mihály J,Németh C,et al.Biochimica Biophysica Acta-Biomembranes,2015,1848(5):1092.
[13] Berényi S,Mihály J,Wacha A,et al.Colloids Surf B Biointerfaces,2014,118(22):164.
[14] Kamiński D M,Arczewska M,Pociecha D,et al.Journal of Molecular Structure,2015,1080:57.
[15] Wu F G,Jia Q,Wu R G,et al.Journal of Physical Chemistry B,2011,115(26):8559.
[16] Wu F G,Wang N N,Yu J S,et al.Journal of Physical Chemistry B,2010,114(6):2158.
[17] Bilge D,Kazanci N,Severcan F.Journal of Molecular Structure,2013,1040(20):75.
[18] Wu R G,Wang Y R,Wu F G,et al.Journal of Thermal Analysis and Calorimetry,2012,109(1):311.
[19] Berényi S,Mihály J,Kristyán S,et al.Biochimica Et Biophysica Acta-Biomembranes,2013,1828(2):661.
[20] Wu R G,Dai J D,Wu F G,et al.Int.J.Pharm.,2012,438(1-2):91.
[21] Pili B,Bourgaux C,Amenitsch H,et al.Biochimica Biophysica Acta-Biomembranes,2010,1798(8):1522.
[22] Kalamkar V,Joshi M,Borkar V,et al.Bioorganic and Medicinal Chemistry,2013,21(21):6753.
[23] Nunes C,Brezesinski G,Lima J L F C,et al.Soft Matter,2011,7(6):3002.