粗毛纤孔菌的化学成分及抗肿瘤活性成分
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摘要
本文研究了粗毛纤孔菌的化学成分及抗肿瘤活性成分。对粗毛纤孔菌的甲醇提取物进行石油醚、乙酸乙酯、正丁醇萃取,采用Sephadex LH-20凝胶色谱法,反相C18柱色谱法及高效液相色谱法对不同萃取组分进行分离纯化。分离得到8个化合物,经鉴定分别为麦角甾醇、齿孔酸、4-(3,4-二羟苯基)-3-丁烯-2-酮、phellibaumin A、3,3?-亚甲基双[6-[2-(3,4-二羟苯基)乙烯基]-4-羟基-2H-吡喃-2-酮](MBP)、肌苷、原儿茶酸和原儿茶醛。其中化合物MBP为首次从自然界中分离得到,对其进行了MTT抗肿瘤筛选和细胞凋亡分析。结果表明此化合物对人肝癌细胞HepG2的细胞增殖具有抑制作用,IC50值为2.3μg/mL,并且可以诱导HepG2细胞凋亡,且呈一定的剂量依赖关系。本研究明确了MBP的提取方法,初步断定该化合物抗肿瘤活性是通过诱导细胞凋亡实现的。
The chemical composition and anti-tumour active compound of Inonotus hispidus were studied. Liquid-liquid distribution using petroleum ether, ethyl acetate, and n-butanol was adopted for extraction of methanol extract of Inonotus hispidus fruiting body. Sephadex LH-20 gel chromatography, reversed-phase C18 column chromatography and high performance liquid chromatography(HPLC) methods were used for further separation and purification of the extract. Eight compounds were isolated and identified, viz. ergosterol, eburicoic acid,(E)-4-(3,4-dihydroxyphenyl) but-3-en-2-one, phellibaumin A, 3,3'-methylene-bis[6-(3,4-dihydroxystyryl)-4-hydroxy-2 H-pyran-2-one](MBP), inosine, protocatechuic acid and protocatechualdehyde. MBP was purified from nature for the first time, and it was used for MTTanti-tumour screening and apoptosis analysis. It was proved that MBP could inhibit the proliferation of HepG2 cells, with half-maximal inhibitory concentration of 2.3μg/mL, and it could induce HepG2 cell apoptosis in a dose-dependent manner.
The chemical composition and anti-tumour active compound of Inonotus hispidus were studied. Liquid-liquid distribution using petroleum ether, ethyl acetate, and n-butanol was adopted for extraction of methanol extract of Inonotus hispidus fruiting body. Sephadex LH-20 gel chromatography, reversed-phase C18 column chromatography and high performance liquid chromatography(HPLC) methods were used for further separation and purification of the extract. Eight compounds were isolated and identified, viz. ergosterol, eburicoic acid,(E)-4-(3,4-dihydroxyphenyl) but-3-en-2-one, phellibaumin A, 3,3'-methylene-bis[6-(3,4-dihydroxystyryl)-4-hydroxy-2 H-pyran-2-one](MBP), inosine, protocatechuic acid and protocatechualdehyde. MBP was purified from nature for the first time, and it was used for MTTanti-tumour screening and apoptosis analysis. It was proved that MBP could inhibit the proliferation of HepG2 cells, with half-maximal inhibitory concentration of 2.3μg/mL, and it could induce HepG2 cell apoptosis in a dose-dependent manner.
引文
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Chen DJ,2009.Hispidin inhibits the growth of human hepatocellular carcinoma cell HepG 2 and its mechanism of inducing apoptosis.PhD Dissertation,China Medical University,Taiwan.1-52(in Chinese)
Chen W,2008.The study of new medicinal shelf fungus fermented powder and antitumor molecular mechanism of hispolon from shelf fungus.PhD Dissertation,Zhejiang University,Hangzhou.1-128(in Chinese)
Cui BK,Dai YC,Yang H,2009.Notes on the medicinal fungus of Inonotus hispidus.Edible Fungi of China,28(4):6-7(in Chinese)
Dai YC,2012.Pathogenic wood-decaying fungi on woody plants in China.Mycosystema,31(4):493-509(in Chinese)
Edgar VV,Rudolf RW,Claus KM.Bishispidine compounds,processes for their production and pharmaceutical agents containing these compounds:United States,US5731344,1995-3-30
Jung JY,2008.Antioxidant polyphenols from the mycelial culture of the medicinal fungi Inonotus xeranticus and Phellinus linteus.Journal of Applied Microbiology,104:1824-1832
Li DH,Du LJ,Kang N,Gu JL,Wang ZB,2018.Effect of extraction techniques on the extraction and hypolipidemic activity in vitro of triterpenoids from Inonotus hispidus.Food Science,39(10):291-297(in Chinese)
Liu B,1974.Chinese medicinal fungi.People’s Publishing House of Shanxi,Taiyuan.71-73(in Chinese)
Liu X,Hou RL,Jin SS,Qi M,Hu KH,Zheng MF,Fu JS,2018.Molecular screening of medicinal fungus Inonotus hispidus and anti-breast cancer activities of its submerged fermentation broth.Mycosystema,37(2):215-225(in Chinese)
Ohmura K,Miyase T,Ueno A,1989.Sesquiterpene glucosides and a phenylbutanoid glycoside from Hypochoeris radicata.Phytochemistry,28(7):1919-1924
Song YX,Wang H,Zhang DH,Yang F,Zhen ZX,Yang GM,2016.Artificial cultivation test of Inonotus hispidus parasitizing on mulberry.Science of Sericulture,42(6):1085-1091(in Chinese)
Wang T,Bao HY,Bau T,Li Y,2016.Biological characteristics and cultivation of Inonotus hispidus parasitizing on Ulmus macrocarpa var.mongolica.Mycosystema,35(6):694-704(in Chinese)
Wu CS,Lin ZM,Wang LN,Guo DX,Wang SQ,Liu YQ,Yuan HQ,Lou HX,2011.Phenolic compounds with NF-?B inhibitory effects from the fungus Phellinus baumii.Bioorganic&Medicinal Chemistry Letters,21:3261-3267
Xie FY,Xu HY,Wang ZB,2012.Biological characteristic of Inonotus hispidus.Journal of Agricultural Science Yanbian University,34(1):36-39(in Chinese)
Yang XZ,2008.Studies on the chemical constituents of Xanthochrous hispidus(Bull.ex Fr.)Pat.Master Thesis,Shandong Traditional Chinese Medicine University,Jinan.1-23(in Chinese)
Ying JZ,Mao XL,Ma QM,Zong YC,Wen AH,1987.Icones of medicinal fungi from China.Science Press,Beijing.1-579(in Chinese)
Yousfi M,Djeridane A,Bombarda I,Chahrazed-Hamia,Duhem B,Gaydou EM,2009.Isolation and characterization of a new hispolone derivative from antioxidant extracts of Pistacia atlantica.Phytotherapy Research,23:1237-1242
Zan LF,2012.Studies on the chemical constituents and their pharmacology actions of Inonotus hispidus and Fomitiporia ellipsoidea fruiting bodies.PhD Dissertation,Jilin Agricultural University,Changchun.1-56(in Chinese)
Zan LF,Bao HY,Bau T,Li Y,Zhu YP,2013.Chemical composition in fruiting body of Inonotus hispidus(Hymenochaetales,Basidiomycota).Mycosystema,32(1):150-156(in Chinese)
Zan LF,Qin JC,Zang YM,Yao YH,Bao HY,Li X,2011.Antioxidant hispidin derivatives from medicinal mushroom Inonotus hispidus.Chemical and Pharmaceutical Bulletin,59(6):770-772
Zhang Y,Bao HY,2014.A comparative study on antitumor activities of polysaccharides from four species of Polyporaceae.Mycosystema,33(1):114-120(in Chinese)
Zhen ZX,Li YX,Wang H,Yang GM,Song YX,2013.An investigation on development and growth environment of wild Phellinus linteus.Science of Sericulture,39(2):333-339(in Chinese)
陈典加,2009.Hispidin对人类肝癌细胞株HepG2之生长抑制及诱导细胞凋亡之机制探讨.中国医药大学博士论文,台湾.1-52
陈卫,2008.层孔菌发酵菌粉的新药材研制及其活性成分Hispolon的抗肿瘤分子机制研究.浙江大学博士学位论文,杭州.1-128
戴玉成,2012.中国木本植物病原木材腐朽菌研究.菌物学报,31(4):493-509
崔宝凯,戴玉成,杨宏,2009.药用真菌粗毛纤孔菌概述.中国食用菌,28(4):6-7
李德海,杜令娟,康宁,顾嘉林,王占斌,2018.提取技术对粗毛纤孔菌三萜类化合物制备及体外降血脂作用的影响.食品科学,39(10):291-297
刘波,1974.中国药用真菌.太原:山西人民出版社.71-73
刘鑫,侯若琳,金珊珊,戚梦,胡开辉,郑明锋,傅俊生,2018.药用真菌粗毛纤孔菌的分子甄别及其发酵液抗乳腺癌活性研究.菌物学报,37(2):215-225
宋永学,王晖,张东豪,杨帆,甄占萱,杨贵明,2016.寄生桑树的粗毛纤孔菌的人工栽培试验.蚕业科学,42(6):1085-1091
王婷,包海鹰,图力古尔,李玉,2016.寄生于蒙古黄榆上的粗毛纤孔菌生物学特性及驯化栽培.菌物学报,35(6):694-704
谢凤颖,徐红云,王占斌,2012.粗毛纤孔菌的菌丝培养特性.延边大学农学学报,34(1):36-39
杨修镇,2008.粗毛黄褐孔菌化学成分及质量控制研究.山东中医药大学硕士学位论文,济南.1-23
应建浙,卯晓岚,马启明,宗毓臣,文华安,1987.中国药用真菌图鉴.北京:科学出版社.1-579
昝立峰,2012.粗毛纤孔菌与椭圆嗜蓝孢孔菌子实体的化学成分及其药理活性研究.吉林农业大学博士学位论文,长春.1-56
昝立峰,包海鹰,图力古尔,李玉,朱艳萍,2013.粗毛纤孔菌子实体化学成分.菌物学报,32(1):150-156
张媛,包海鹰,2014.四种多孔菌子实体粗多糖抗肿瘤活性的比较研究.菌物学报,33(1):114-120
甄占萱,李云祥,王晖,杨贵明,宋永学,2013.野生桑黄的生长发育及环境调查.蚕业科学,39(2):333-339
Chen DJ,2009.Hispidin inhibits the growth of human hepatocellular carcinoma cell HepG 2 and its mechanism of inducing apoptosis.PhD Dissertation,China Medical University,Taiwan.1-52(in Chinese)
Chen W,2008.The study of new medicinal shelf fungus fermented powder and antitumor molecular mechanism of hispolon from shelf fungus.PhD Dissertation,Zhejiang University,Hangzhou.1-128(in Chinese)
Cui BK,Dai YC,Yang H,2009.Notes on the medicinal fungus of Inonotus hispidus.Edible Fungi of China,28(4):6-7(in Chinese)
Dai YC,2012.Pathogenic wood-decaying fungi on woody plants in China.Mycosystema,31(4):493-509(in Chinese)
Edgar VV,Rudolf RW,Claus KM.Bishispidine compounds,processes for their production and pharmaceutical agents containing these compounds:United States,US5731344,1995-3-30
Jung JY,2008.Antioxidant polyphenols from the mycelial culture of the medicinal fungi Inonotus xeranticus and Phellinus linteus.Journal of Applied Microbiology,104:1824-1832
Li DH,Du LJ,Kang N,Gu JL,Wang ZB,2018.Effect of extraction techniques on the extraction and hypolipidemic activity in vitro of triterpenoids from Inonotus hispidus.Food Science,39(10):291-297(in Chinese)
Liu B,1974.Chinese medicinal fungi.People’s Publishing House of Shanxi,Taiyuan.71-73(in Chinese)
Liu X,Hou RL,Jin SS,Qi M,Hu KH,Zheng MF,Fu JS,2018.Molecular screening of medicinal fungus Inonotus hispidus and anti-breast cancer activities of its submerged fermentation broth.Mycosystema,37(2):215-225(in Chinese)
Ohmura K,Miyase T,Ueno A,1989.Sesquiterpene glucosides and a phenylbutanoid glycoside from Hypochoeris radicata.Phytochemistry,28(7):1919-1924
Song YX,Wang H,Zhang DH,Yang F,Zhen ZX,Yang GM,2016.Artificial cultivation test of Inonotus hispidus parasitizing on mulberry.Science of Sericulture,42(6):1085-1091(in Chinese)
Wang T,Bao HY,Bau T,Li Y,2016.Biological characteristics and cultivation of Inonotus hispidus parasitizing on Ulmus macrocarpa var.mongolica.Mycosystema,35(6):694-704(in Chinese)
Wu CS,Lin ZM,Wang LN,Guo DX,Wang SQ,Liu YQ,Yuan HQ,Lou HX,2011.Phenolic compounds with NF-?B inhibitory effects from the fungus Phellinus baumii.Bioorganic&Medicinal Chemistry Letters,21:3261-3267
Xie FY,Xu HY,Wang ZB,2012.Biological characteristic of Inonotus hispidus.Journal of Agricultural Science Yanbian University,34(1):36-39(in Chinese)
Yang XZ,2008.Studies on the chemical constituents of Xanthochrous hispidus(Bull.ex Fr.)Pat.Master Thesis,Shandong Traditional Chinese Medicine University,Jinan.1-23(in Chinese)
Ying JZ,Mao XL,Ma QM,Zong YC,Wen AH,1987.Icones of medicinal fungi from China.Science Press,Beijing.1-579(in Chinese)
Yousfi M,Djeridane A,Bombarda I,Chahrazed-Hamia,Duhem B,Gaydou EM,2009.Isolation and characterization of a new hispolone derivative from antioxidant extracts of Pistacia atlantica.Phytotherapy Research,23:1237-1242
Zan LF,2012.Studies on the chemical constituents and their pharmacology actions of Inonotus hispidus and Fomitiporia ellipsoidea fruiting bodies.PhD Dissertation,Jilin Agricultural University,Changchun.1-56(in Chinese)
Zan LF,Bao HY,Bau T,Li Y,Zhu YP,2013.Chemical composition in fruiting body of Inonotus hispidus(Hymenochaetales,Basidiomycota).Mycosystema,32(1):150-156(in Chinese)
Zan LF,Qin JC,Zang YM,Yao YH,Bao HY,Li X,2011.Antioxidant hispidin derivatives from medicinal mushroom Inonotus hispidus.Chemical and Pharmaceutical Bulletin,59(6):770-772
Zhang Y,Bao HY,2014.A comparative study on antitumor activities of polysaccharides from four species of Polyporaceae.Mycosystema,33(1):114-120(in Chinese)
Zhen ZX,Li YX,Wang H,Yang GM,Song YX,2013.An investigation on development and growth environment of wild Phellinus linteus.Science of Sericulture,39(2):333-339(in Chinese)
陈典加,2009.Hispidin对人类肝癌细胞株HepG2之生长抑制及诱导细胞凋亡之机制探讨.中国医药大学博士论文,台湾.1-52
陈卫,2008.层孔菌发酵菌粉的新药材研制及其活性成分Hispolon的抗肿瘤分子机制研究.浙江大学博士学位论文,杭州.1-128
戴玉成,2012.中国木本植物病原木材腐朽菌研究.菌物学报,31(4):493-509
崔宝凯,戴玉成,杨宏,2009.药用真菌粗毛纤孔菌概述.中国食用菌,28(4):6-7
李德海,杜令娟,康宁,顾嘉林,王占斌,2018.提取技术对粗毛纤孔菌三萜类化合物制备及体外降血脂作用的影响.食品科学,39(10):291-297
刘波,1974.中国药用真菌.太原:山西人民出版社.71-73
刘鑫,侯若琳,金珊珊,戚梦,胡开辉,郑明锋,傅俊生,2018.药用真菌粗毛纤孔菌的分子甄别及其发酵液抗乳腺癌活性研究.菌物学报,37(2):215-225
宋永学,王晖,张东豪,杨帆,甄占萱,杨贵明,2016.寄生桑树的粗毛纤孔菌的人工栽培试验.蚕业科学,42(6):1085-1091
王婷,包海鹰,图力古尔,李玉,2016.寄生于蒙古黄榆上的粗毛纤孔菌生物学特性及驯化栽培.菌物学报,35(6):694-704
谢凤颖,徐红云,王占斌,2012.粗毛纤孔菌的菌丝培养特性.延边大学农学学报,34(1):36-39
杨修镇,2008.粗毛黄褐孔菌化学成分及质量控制研究.山东中医药大学硕士学位论文,济南.1-23
应建浙,卯晓岚,马启明,宗毓臣,文华安,1987.中国药用真菌图鉴.北京:科学出版社.1-579
昝立峰,2012.粗毛纤孔菌与椭圆嗜蓝孢孔菌子实体的化学成分及其药理活性研究.吉林农业大学博士学位论文,长春.1-56
昝立峰,包海鹰,图力古尔,李玉,朱艳萍,2013.粗毛纤孔菌子实体化学成分.菌物学报,32(1):150-156
张媛,包海鹰,2014.四种多孔菌子实体粗多糖抗肿瘤活性的比较研究.菌物学报,33(1):114-120
甄占萱,李云祥,王晖,杨贵明,宋永学,2013.野生桑黄的生长发育及环境调查.蚕业科学,39(2):333-339