微生物对饮用水典型消毒副产物前体物的降解效能及其群落特征
|
摘要
相对于CF (氯仿)等C-DBPs (含碳消毒副产物),DCAN (二氯乙腈)等N-DBPs (含氮消毒副产物)具有更高的毒性.控制DBPs (消毒副产物)的前体物是抑制DBPs产生的最有效方法之一.为考察微生物降解DBPs前体物对生成DBPs的影响,分别选取C-DBPs和N-DBPs的典型代表物CF和DCAN及其相应的典型前体物Tyr (酪氨酸)和Asp (天冬氨酸)为研究对象,采用微生物培养前体物的方式,探究微生物对典型前体物Tyr和Asp的降解效果及其对生成CF和DCAN的控制效果.结果表明:①Tyr和Asp两种前体物经微生物降解后,DOC (溶解性有机碳)的去除率分别为94. 0%和85. 6%,DON (溶解性有机氮)的去除率分别为69. 0%和81. 0%.②在前体物经微生物降解后的水样中,氯化或氯胺化消毒后生成CF和DCAN的量较降解前均大大减少.以Tyr为前体物,水样经微生物降解、氯化消毒后生成CF和DCAN的量分别降低了56. 1%和89. 5%,氯胺化消毒后生成CF的量降低了68. 5%;以Asp为前体物,水样经微生物降解、氯化消毒后生成DCAN的量最高可降低99. 9%,经微生物降解、氯胺化消毒后生成的CF可降低50. 7%.③对水样中微生物菌群分析发现,在门水平上的菌群主要有变形菌门(Proteobacteria)、放线菌门(Actinobacteria)和拟杆菌门(Bacteroidetes),在属水平上的菌属主要有伯克氏菌属(Burkholderia-paraburkholderia)、半角藻属(Haliangium)、分枝杆菌属(Mycobacterium)、沉积小杆菌属(Sediminibacterium)、norank_f_Chitinophagaceae和动胶菌属(Zoogloea).研究显示,微生物降解对DBPs典型前体物Tyr和Asp的去除,以及对生成CF和DCAN的控制具有较大的潜力,变形菌和放线菌在降解DBPs前体物中起到了重要作用.
N-DBPs( e.g. DCAN) have received more attention in recent years,which were reported to be more toxic than C-DBPs( e.g.CF). One of the most effective ways to control the formation of DBPs was to remove its precursors before disinfection. To investigate the effect of microbial degradation on the formation of DBPs during subsequent chlorination and chloramination from DBPs precursors,this study selected CF and DCAN as the representative C-DBPs and N-DBPs,and selected Asp( aspartic acid) and Tyr( tyrosine) as the typical precursors to explore the effect of microbial degradation on the formation of DBPs. The results showed that after the degradation of Tyr and Asp,the removal rate of DOC was 94. 0% and 85. 6%,respectively,and the removal rate of DON was 69. 0% and 81. 0%,respectively. The amount of CF and DCAN during chlor( am) ination decreased greatly after biodegradation. The highest removal rates of CF and DCAN were reached 56. 1% and 89. 5% respectively when Tyr water sample was chlorinated. And the amount of CF was reduced by 68. 5% for Tyr water sample during chloramination. For Asp water sample,the removal rate of DCAN reached 99. 9% during chlorination,and the amount of CF reduced by 50. 7% during chloramination. The microbial communities in water samples were analyzed.At the phylum level,Proteobacteria、Actinobacteria and Bacteroidetes were the dominant phylum. At the genus level,Burkholderiaparaburkholderia、Haliangium、Mycobacterium、Sediminibacterium、norank_f_chitinophagaceae and Zoogloea were the dominant genus.The research showed that biodegradation has great potential in controlling the formation of C-DBPs and N-DBPs. It is necessary to carry out the research on the control of multi-DBPs precursors by microbial degradation to realize the integrated control of DBPs.
N-DBPs( e.g. DCAN) have received more attention in recent years,which were reported to be more toxic than C-DBPs( e.g.CF). One of the most effective ways to control the formation of DBPs was to remove its precursors before disinfection. To investigate the effect of microbial degradation on the formation of DBPs during subsequent chlorination and chloramination from DBPs precursors,this study selected CF and DCAN as the representative C-DBPs and N-DBPs,and selected Asp( aspartic acid) and Tyr( tyrosine) as the typical precursors to explore the effect of microbial degradation on the formation of DBPs. The results showed that after the degradation of Tyr and Asp,the removal rate of DOC was 94. 0% and 85. 6%,respectively,and the removal rate of DON was 69. 0% and 81. 0%,respectively. The amount of CF and DCAN during chlor( am) ination decreased greatly after biodegradation. The highest removal rates of CF and DCAN were reached 56. 1% and 89. 5% respectively when Tyr water sample was chlorinated. And the amount of CF was reduced by 68. 5% for Tyr water sample during chloramination. For Asp water sample,the removal rate of DCAN reached 99. 9% during chlorination,and the amount of CF reduced by 50. 7% during chloramination. The microbial communities in water samples were analyzed.At the phylum level,Proteobacteria、Actinobacteria and Bacteroidetes were the dominant phylum. At the genus level,Burkholderiaparaburkholderia、Haliangium、Mycobacterium、Sediminibacterium、norank_f_chitinophagaceae and Zoogloea were the dominant genus.The research showed that biodegradation has great potential in controlling the formation of C-DBPs and N-DBPs. It is necessary to carry out the research on the control of multi-DBPs precursors by microbial degradation to realize the integrated control of DBPs.
引文
[1] PLEWA M J,WAGNER E D. Charting a new path to resolve the adverse health effects of DBPs[J]. American Chemical Society,2015,1:3-23.
[2] PLEWA M J,WAGNER E D,RICHARDSON S D. TIC-Tox:a preliminary discussion on identifying the forcing agents of DBPmediated toxicity of disinfected water[J].Journal of Environmental Sciences,2017,58(8):208-216.
[3] RICHARDSON S D,PLEWA M J,WAGNER E D,et al.Occurrence,genotoxicity,and carcinogenicity of regulated and emerging disinfection by-products in drinking water:a review and roadmap for research[J]. Mutation Research,2007,636(1/2/3):178-242.
[4] XIA Chufan,MA Defang,GAO Baoyu,et al. Characteristics and trihalomethane formation reactivity of dissolved organic matter in effluents from membrane bioreactors with and without filamentous bulking[J].Bioresource Technology,2016,211:183-189.
[5] RECKHOW D A,MACNEILL A L,PLATT T L,et al. Formation and degradation of dichloroacetonitrile in drinking waters[J].Journal of Water Supply:Research and Technology,2001,50(1):1-13.
[6] KRASNER S W,WEINBERG H S,RICHARDSON S D,et al.Occurrence of a new generation of disinfection byproducts[J].Environmental Science&Technology,2006,40(23):7175-7185.
[7] PLEWA M J.Comparative mammalian cell toxicity of N-DBPs and C-DBPs[J].Acs Symposium,2008,995:36-50.
[8] MUELLNER M G,WAGNER E D,MCCALLA K.Haloacetonitriles vs. regulated haloacetic acids:are nitrogen-containing DBPs more toxic[J].Environmental Science&Technology,2007,41(2):645-651.
[9]楚文海,高乃云,赵世嘏,等.溶解性有机氮乙酰胺氯化生成饮用水THMs的影响因素研究[J].环境科学,2009,30(5):1376-1380.CHU Wenhai,GAO Naiyun,ZHAO Shijia,et al. Factors affecting formation of THMs during dissolved organic nitrogen acetamide chlorination in drinking water.[J]. Environmental Science,2009,30(5):1376-1380.
[10] SHAH A D,MITCH W A. Halonitroalkanes,halonitriles,haloamides,and N-nitrosamines:a critical review of nitrogenous disinfection byproduct formation pathways[J]. Environmental Science&Technology,2012,46(1):119-131.
[11] LEE W,WESTERHOFF P,CROUE J P.Dissolved organic nitrogen as a precursor for chloroform, dichloroacetonitrile,N-nitrosodimethylamine, and trichloronitromethane[J].Environmental Science&Technology,2007,41(15):5485-5490.
[12] CHU Weihai,GAO Naiyun,DENG Yang,et al. Precursors of dichloroacetamide,an emerging nitrogenous DBP formed during chlorination or chloramination[J]. Environmental Science&Technology,2010,44(10):3908-3912.
[13] BOND T,HUANG Jin,TEMPLETON M R,et al. Occurrence and control of nitrogenous disinfection by-products in drinking water:a review[J].Water Research,2011,45(15):4341-4354.
[14] DOTSON A,WESTERHOFF P. Occurrence and removal of amino acids during drinking water treatment[J]. American Water Works Association,2009,101(9):101-115.
[15] LEE W,WESTERHOFF P. Dissolved organic nitrogen removal during water treatment by aluminum sulfate and cationic polymer coagulation[J].Water Research,2006,40(20):3767-3774.
[16] VON G U.Ozonation of drinking water:partⅡ.disinfection and byproduct formation in presence of bromide,iodide or chlorine[J].Water Research,2003,37(7):1469-1487.
[17] CHU Weihai,LI Dongmei,GAO Naiyun,et al. Comparison of free amino acids and short oligopeptides for the formation of trihalomethanes and haloacetonitriles during chlorination:effect of peptide bond and pre-oxidation[J].Chemical Engineering Journal,2015,281:623-631.
[18] CHU Weihai,LI Changjun,GAO Naiyun,et al. Terminating preozonation prior to biological activated carbon filtration results in increased formation of nitrogenous disinfection by-products upon subsequent chlorination[J].Chemosphere,2015,121(2):33-38.
[19] CHU Weihai,GAO Naiyun,YIN Daqiang,et al. Ozone-biological activated carbon integrated treatment for removal of precursors of halogenated nitrogenous disinfection by-products[J].Chemosphere,2012,86(11):1087-1091.
[20] CHU Weihai,GAO Naiyun,DENG Yang,et al.Impacts of drinking water pretreatments on the formation of nitrogenous disinfection byproducts[J]. Bioresource Technology,2011,102(24):11161-11166.
[21] HUANG Chao,LUO Mutan,CHEN Xuefang,et al.Recent advances and industrial viewpoint for biological treatment of wastewaters by oleaginous microorganisms[J].Bioresource Technology,2017,232:398-407.
[22] SERRANO M,CHANDRA R,CASTILLO-ZACARIAS C,et al.Biotransformation and degradation of 2,4,6-trinitrotoluene by microbial metabolism and their interaction[J]. Defence Technology,2018,14(2):151-164.
[23] DE-VERA G A,KELLER J,GERNJAK W,et al.Biodegradability of DBP precursors after drinking water ozonation[J].Water Research,2016,106:550-561.
[24] CHU Weihai,GAO Naiyun,KRASNER S W,et al. Formation of halogenated C-,N-DBPs from chlor(am)ination and UV irradiation of tyrosine in drinking water[J]. Environmental Pollution,2012,161(1):8-14.
[25] SANAPAREDDY N,HAMP T J,GONZALEZ L C,et al.Molecular diversity of a north carolina wastewater treatment plant as revealed by pyrosequencing[J]. Applied&Environmental Microbiology,2009,75(6):1688-1696.
[26] RATPUKDI T,SIRIPATTANAKUL S,KHAN E.Mineralization and biodegradability enhancement of natural organic matter by ozoneVUV in comparison with ozone,VUV,ozone-UV,and UV:effects of p H and ozone dose[J]. Water Research,2010,44(11):3531-3543.
[27] KHAN E,SY-SAVANE O,JITTAWATTANARAT R.Application of commercial biochemical oxygen demand inocula for biodegradable dissolved organic carbon determination[J]. Water Research,2005,39(19):4824-4834.
[28] WADHAWAN T,SIMSEK H,KASI M,et al. Dissolved organic nitrogen and its biodegradable portion in a water treatment plant with ozone oxidation[J].Water Research,2014,54(5):318-326.
[29] TERRY L G,SUMMERS R S. Biodegradable organic matter and rapid-rate biofilter performance:a review[J]. Water Research,2018,128:234-245.
[30] DIAS F F,ALEXANDER M. Effect of chemical structure on the biodegradability of aliphatic acids and alcohols[J]. Applied Microbiology,1971,22(6):1114-1118.
[31] DU Zhe,CHEN Yinguang,LI Xu.Quantitative proteomic analyses of the microbial degradation of estrone under various background nitrogen and carbon conditions[J]. Water Research,2017,123:361-368.
[32] HUREIKI L,CROUJ P,LEGUBE B.Chlorination studies of free and combined amino acids[J]. Water Research,1994,28(12):2521-2531.
[33] BOND T,MOKHTAR-KAMAL N H,BONNISSEAU T,et al.Disinfection by-product formation from the chlorination and chloramination of amines[J].Journal of Hazardous Materials,2014,278:288-296.
[34] XU Bin,GAO Naiyun,SUN Xiaofeng,et al. Characteristics of organic material in Huangpu River and treatability with the O3-BAC process[J]. Separation&Purification Technology,2007,57(2):348-355.
[35] UENO H,MOTO T,SAYATO Y,et al.Disinfection by-products in the chlorination of organic nitrogen compounds:by-products from kynurenine[J].Chemosphere,1996,33(8):1425-1433.
[36] HU Jianglin,CHU Weihai,SUI Minghao,et al. Comparison of drinking water treatment processes combinations for the minimization of subsequent disinfection by-products formation during chlorination and chloramination[J]. Chemical Engineering Journal,2018,335:352-361.
[37] HU Man,WANG Xiaohui,WEN Xianghua,et al. Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis[J]. Bioresource Technology,2012,117(10):72-79.
[38] KOLEHMAINEN R E,TIIROLA M,PUHAKKA J A. Spatial and temporal changes in actinobacterial dominance in experimental artificial groundwater recharge[J].Water Research,2008,42(17):4525-4537.
[39] SUI Qianwen,LIU Chong,ZHANG Junya,et al.Response of nitrite accumulation and microbial community to free ammonia and dissolved oxygen treatment of high ammonium wastewater[J].Applied Microbiology&Biotechnology,2016,100(9):4177-4187.
[40] BECERRA-CASTRO C,MACEDO G,SILVA A M T,et al.Proteobacteria,become predominant during regrowth after water disinfection[J].Science of the Total Environment,2016,573:313-323.
[41] SHI Peng,JIA Shuyu,ZHANG Xuxiang,et al.Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water[J].Water Research,2013,47(1):111-120.
[42] INGLIS T J J,RIGBY P,ROBERTSON T A,et al. Interaction between Burkholderia pseudomallei and acanthamoeba species results in coiling phagocytosis,endamebic bacterial survival,and escape[J].Infection&Immunity,2000,68(3):1681-1686.
[43] HOWARD K,INGLIS T J J. Disinfection of burkholderia pseudomallei,in potable water[J]. Water Research,2005,39(6):1085-1092.
[44] WANG Ping,YU Zhisheng,QI Rong,et al.Detailed comparison of bacterial communities during seasonal sludge bulking in a municipal wastewater treatment plant[J]. Water Research,2016,105:157-166.
[45] GUO Ning,WANG Yunkun,TONG Tiezheng,et al. The fate of antibiotic resistance genes and their potential hosts during bioelectrochemical treatment of high-salinity pharmaceutical wastewater[J].Water Research,2018,133:79-86.
[46] QU Jianhang, YUAN Hongli. Sediminibacterium salmoneum gen.nov.,sp.nov.,a member of the phylum bacteroidetes isolated from sediment of a eutrophic reservoir[J]. International Journal of Systematic&Evolutionary Microbiology,2008,58(9):2191-2194.
[47] WANG Haibo,HU Chun,HU Xuexiang,et al.Effects of disinfectant and biofilm on the corrosion of cast iron pipes in a reclaimed water distribution system[J].Water Research,2012,46(4):1070-1078.
[48] MA Qiao,QU Yuanyuan,SHEN Weili,et al. Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing[J]. Bioresource Technology,2015,179:436-443.
[49] WANG Xiaohui,HU Man,XIA Yu,et al.Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China[J].Applied&Environmental Microbiology,2012,78(19):7042-7049.
[50] ZHANG Tong,SHAO Mingfei,YE Lin. 454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants[J].Isme Journal,2012,6(6):1137-1147.
[2] PLEWA M J,WAGNER E D,RICHARDSON S D. TIC-Tox:a preliminary discussion on identifying the forcing agents of DBPmediated toxicity of disinfected water[J].Journal of Environmental Sciences,2017,58(8):208-216.
[3] RICHARDSON S D,PLEWA M J,WAGNER E D,et al.Occurrence,genotoxicity,and carcinogenicity of regulated and emerging disinfection by-products in drinking water:a review and roadmap for research[J]. Mutation Research,2007,636(1/2/3):178-242.
[4] XIA Chufan,MA Defang,GAO Baoyu,et al. Characteristics and trihalomethane formation reactivity of dissolved organic matter in effluents from membrane bioreactors with and without filamentous bulking[J].Bioresource Technology,2016,211:183-189.
[5] RECKHOW D A,MACNEILL A L,PLATT T L,et al. Formation and degradation of dichloroacetonitrile in drinking waters[J].Journal of Water Supply:Research and Technology,2001,50(1):1-13.
[6] KRASNER S W,WEINBERG H S,RICHARDSON S D,et al.Occurrence of a new generation of disinfection byproducts[J].Environmental Science&Technology,2006,40(23):7175-7185.
[7] PLEWA M J.Comparative mammalian cell toxicity of N-DBPs and C-DBPs[J].Acs Symposium,2008,995:36-50.
[8] MUELLNER M G,WAGNER E D,MCCALLA K.Haloacetonitriles vs. regulated haloacetic acids:are nitrogen-containing DBPs more toxic[J].Environmental Science&Technology,2007,41(2):645-651.
[9]楚文海,高乃云,赵世嘏,等.溶解性有机氮乙酰胺氯化生成饮用水THMs的影响因素研究[J].环境科学,2009,30(5):1376-1380.CHU Wenhai,GAO Naiyun,ZHAO Shijia,et al. Factors affecting formation of THMs during dissolved organic nitrogen acetamide chlorination in drinking water.[J]. Environmental Science,2009,30(5):1376-1380.
[10] SHAH A D,MITCH W A. Halonitroalkanes,halonitriles,haloamides,and N-nitrosamines:a critical review of nitrogenous disinfection byproduct formation pathways[J]. Environmental Science&Technology,2012,46(1):119-131.
[11] LEE W,WESTERHOFF P,CROUE J P.Dissolved organic nitrogen as a precursor for chloroform, dichloroacetonitrile,N-nitrosodimethylamine, and trichloronitromethane[J].Environmental Science&Technology,2007,41(15):5485-5490.
[12] CHU Weihai,GAO Naiyun,DENG Yang,et al. Precursors of dichloroacetamide,an emerging nitrogenous DBP formed during chlorination or chloramination[J]. Environmental Science&Technology,2010,44(10):3908-3912.
[13] BOND T,HUANG Jin,TEMPLETON M R,et al. Occurrence and control of nitrogenous disinfection by-products in drinking water:a review[J].Water Research,2011,45(15):4341-4354.
[14] DOTSON A,WESTERHOFF P. Occurrence and removal of amino acids during drinking water treatment[J]. American Water Works Association,2009,101(9):101-115.
[15] LEE W,WESTERHOFF P. Dissolved organic nitrogen removal during water treatment by aluminum sulfate and cationic polymer coagulation[J].Water Research,2006,40(20):3767-3774.
[16] VON G U.Ozonation of drinking water:partⅡ.disinfection and byproduct formation in presence of bromide,iodide or chlorine[J].Water Research,2003,37(7):1469-1487.
[17] CHU Weihai,LI Dongmei,GAO Naiyun,et al. Comparison of free amino acids and short oligopeptides for the formation of trihalomethanes and haloacetonitriles during chlorination:effect of peptide bond and pre-oxidation[J].Chemical Engineering Journal,2015,281:623-631.
[18] CHU Weihai,LI Changjun,GAO Naiyun,et al. Terminating preozonation prior to biological activated carbon filtration results in increased formation of nitrogenous disinfection by-products upon subsequent chlorination[J].Chemosphere,2015,121(2):33-38.
[19] CHU Weihai,GAO Naiyun,YIN Daqiang,et al. Ozone-biological activated carbon integrated treatment for removal of precursors of halogenated nitrogenous disinfection by-products[J].Chemosphere,2012,86(11):1087-1091.
[20] CHU Weihai,GAO Naiyun,DENG Yang,et al.Impacts of drinking water pretreatments on the formation of nitrogenous disinfection byproducts[J]. Bioresource Technology,2011,102(24):11161-11166.
[21] HUANG Chao,LUO Mutan,CHEN Xuefang,et al.Recent advances and industrial viewpoint for biological treatment of wastewaters by oleaginous microorganisms[J].Bioresource Technology,2017,232:398-407.
[22] SERRANO M,CHANDRA R,CASTILLO-ZACARIAS C,et al.Biotransformation and degradation of 2,4,6-trinitrotoluene by microbial metabolism and their interaction[J]. Defence Technology,2018,14(2):151-164.
[23] DE-VERA G A,KELLER J,GERNJAK W,et al.Biodegradability of DBP precursors after drinking water ozonation[J].Water Research,2016,106:550-561.
[24] CHU Weihai,GAO Naiyun,KRASNER S W,et al. Formation of halogenated C-,N-DBPs from chlor(am)ination and UV irradiation of tyrosine in drinking water[J]. Environmental Pollution,2012,161(1):8-14.
[25] SANAPAREDDY N,HAMP T J,GONZALEZ L C,et al.Molecular diversity of a north carolina wastewater treatment plant as revealed by pyrosequencing[J]. Applied&Environmental Microbiology,2009,75(6):1688-1696.
[26] RATPUKDI T,SIRIPATTANAKUL S,KHAN E.Mineralization and biodegradability enhancement of natural organic matter by ozoneVUV in comparison with ozone,VUV,ozone-UV,and UV:effects of p H and ozone dose[J]. Water Research,2010,44(11):3531-3543.
[27] KHAN E,SY-SAVANE O,JITTAWATTANARAT R.Application of commercial biochemical oxygen demand inocula for biodegradable dissolved organic carbon determination[J]. Water Research,2005,39(19):4824-4834.
[28] WADHAWAN T,SIMSEK H,KASI M,et al. Dissolved organic nitrogen and its biodegradable portion in a water treatment plant with ozone oxidation[J].Water Research,2014,54(5):318-326.
[29] TERRY L G,SUMMERS R S. Biodegradable organic matter and rapid-rate biofilter performance:a review[J]. Water Research,2018,128:234-245.
[30] DIAS F F,ALEXANDER M. Effect of chemical structure on the biodegradability of aliphatic acids and alcohols[J]. Applied Microbiology,1971,22(6):1114-1118.
[31] DU Zhe,CHEN Yinguang,LI Xu.Quantitative proteomic analyses of the microbial degradation of estrone under various background nitrogen and carbon conditions[J]. Water Research,2017,123:361-368.
[32] HUREIKI L,CROUJ P,LEGUBE B.Chlorination studies of free and combined amino acids[J]. Water Research,1994,28(12):2521-2531.
[33] BOND T,MOKHTAR-KAMAL N H,BONNISSEAU T,et al.Disinfection by-product formation from the chlorination and chloramination of amines[J].Journal of Hazardous Materials,2014,278:288-296.
[34] XU Bin,GAO Naiyun,SUN Xiaofeng,et al. Characteristics of organic material in Huangpu River and treatability with the O3-BAC process[J]. Separation&Purification Technology,2007,57(2):348-355.
[35] UENO H,MOTO T,SAYATO Y,et al.Disinfection by-products in the chlorination of organic nitrogen compounds:by-products from kynurenine[J].Chemosphere,1996,33(8):1425-1433.
[36] HU Jianglin,CHU Weihai,SUI Minghao,et al. Comparison of drinking water treatment processes combinations for the minimization of subsequent disinfection by-products formation during chlorination and chloramination[J]. Chemical Engineering Journal,2018,335:352-361.
[37] HU Man,WANG Xiaohui,WEN Xianghua,et al. Microbial community structures in different wastewater treatment plants as revealed by 454-pyrosequencing analysis[J]. Bioresource Technology,2012,117(10):72-79.
[38] KOLEHMAINEN R E,TIIROLA M,PUHAKKA J A. Spatial and temporal changes in actinobacterial dominance in experimental artificial groundwater recharge[J].Water Research,2008,42(17):4525-4537.
[39] SUI Qianwen,LIU Chong,ZHANG Junya,et al.Response of nitrite accumulation and microbial community to free ammonia and dissolved oxygen treatment of high ammonium wastewater[J].Applied Microbiology&Biotechnology,2016,100(9):4177-4187.
[40] BECERRA-CASTRO C,MACEDO G,SILVA A M T,et al.Proteobacteria,become predominant during regrowth after water disinfection[J].Science of the Total Environment,2016,573:313-323.
[41] SHI Peng,JIA Shuyu,ZHANG Xuxiang,et al.Metagenomic insights into chlorination effects on microbial antibiotic resistance in drinking water[J].Water Research,2013,47(1):111-120.
[42] INGLIS T J J,RIGBY P,ROBERTSON T A,et al. Interaction between Burkholderia pseudomallei and acanthamoeba species results in coiling phagocytosis,endamebic bacterial survival,and escape[J].Infection&Immunity,2000,68(3):1681-1686.
[43] HOWARD K,INGLIS T J J. Disinfection of burkholderia pseudomallei,in potable water[J]. Water Research,2005,39(6):1085-1092.
[44] WANG Ping,YU Zhisheng,QI Rong,et al.Detailed comparison of bacterial communities during seasonal sludge bulking in a municipal wastewater treatment plant[J]. Water Research,2016,105:157-166.
[45] GUO Ning,WANG Yunkun,TONG Tiezheng,et al. The fate of antibiotic resistance genes and their potential hosts during bioelectrochemical treatment of high-salinity pharmaceutical wastewater[J].Water Research,2018,133:79-86.
[46] QU Jianhang, YUAN Hongli. Sediminibacterium salmoneum gen.nov.,sp.nov.,a member of the phylum bacteroidetes isolated from sediment of a eutrophic reservoir[J]. International Journal of Systematic&Evolutionary Microbiology,2008,58(9):2191-2194.
[47] WANG Haibo,HU Chun,HU Xuexiang,et al.Effects of disinfectant and biofilm on the corrosion of cast iron pipes in a reclaimed water distribution system[J].Water Research,2012,46(4):1070-1078.
[48] MA Qiao,QU Yuanyuan,SHEN Weili,et al. Bacterial community compositions of coking wastewater treatment plants in steel industry revealed by Illumina high-throughput sequencing[J]. Bioresource Technology,2015,179:436-443.
[49] WANG Xiaohui,HU Man,XIA Yu,et al.Pyrosequencing analysis of bacterial diversity in 14 wastewater treatment systems in China[J].Applied&Environmental Microbiology,2012,78(19):7042-7049.
[50] ZHANG Tong,SHAO Mingfei,YE Lin. 454 pyrosequencing reveals bacterial diversity of activated sludge from 14 sewage treatment plants[J].Isme Journal,2012,6(6):1137-1147.