大豆Glyma08g11030基因结构及原核表达分析
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摘要
为了进一步明确Glyma08g11030基因的功能,利用生物信息学和PCR的方法,对Glyma08g11030进行序列分析及蛋白结构域分析。生物信息学分析表明,该基因cDNA全长1 419 bp,开放阅读框长1 362 bp,编码453个氨基酸,蛋白质分子质量约为51.288 ku,等电点为8.14。序列分析表明,Glyma08g11030基因组包含2个外显子和1个内含子。多序列比对结果表明,Glyma08g11030蛋白含有1个F-BOX保守域。进化树分析表明,该蛋白与木豆、菜豆、花生、豇豆、红小豆具有同源关系。为了获得纯化的Glyma08g11030蛋白,将扩增片段克隆至原核表达载体pET28a,构建重组质粒pET28a-Glyma08g11030,经过SDS-PAGE电泳检测获得了目的蛋白,结果表明,该蛋白主要以包涵体形式存在。为进一步纯化和鉴定Glyma08g11030蛋白及研究其功能奠定了基础。
In order to further clarify the function of Glyma08 g11030 gene,sequence analysis and protein domain analysis of Glyma08 g11030 were performed by means of bioinformatics and PCR. Bioinformatics analysis showed that the full-length of Glyma08 g11030 cDNA was 1 419 bp,with an open reading frame of 1 362 bp,encoding 453 amino acids. The molecular weight of the protein was about 51.288 ku,and the isoelectric point was 8.14. Sequence analysis indicated that the Glyma08 g11030 genome contained two exons and one intron. Multiple sequence alignment revealed that the Glyma08 g11030 protein contained one F-BOX conserved domain. Phylogenetic tree analysis showed that the protein had a homologous relationship with pigeonpea,kidney bean,peanut,cowpea and adzuki bean. In order to obtain the purified Glyma08 g11030 protein,the amplified fragment was cloned into the prokaryotic expression vector pET28 a,and the recombinant plasmid pET28 a-Glyma08 g11030 was constructed. The target protein was obtained by SDS-PAGE. The results showed that the protein mainly existed in the form of inclusion bodies. The results of this study laid the foundation for further purification and identification of Glyma08 g11030 protein and investigation of its function.
In order to further clarify the function of Glyma08 g11030 gene,sequence analysis and protein domain analysis of Glyma08 g11030 were performed by means of bioinformatics and PCR. Bioinformatics analysis showed that the full-length of Glyma08 g11030 cDNA was 1 419 bp,with an open reading frame of 1 362 bp,encoding 453 amino acids. The molecular weight of the protein was about 51.288 ku,and the isoelectric point was 8.14. Sequence analysis indicated that the Glyma08 g11030 genome contained two exons and one intron. Multiple sequence alignment revealed that the Glyma08 g11030 protein contained one F-BOX conserved domain. Phylogenetic tree analysis showed that the protein had a homologous relationship with pigeonpea,kidney bean,peanut,cowpea and adzuki bean. In order to obtain the purified Glyma08 g11030 protein,the amplified fragment was cloned into the prokaryotic expression vector pET28 a,and the recombinant plasmid pET28 a-Glyma08 g11030 was constructed. The target protein was obtained by SDS-PAGE. The results showed that the protein mainly existed in the form of inclusion bodies. The results of this study laid the foundation for further purification and identification of Glyma08 g11030 protein and investigation of its function.
引文
[1] 韩强.抗虫及抗除草剂转基因大豆新品种的培育与鉴定[D].杭州:浙江大学,2015.Han Q.Cultivation and identification of new varieties of transgenic soybeans resistant to insects and herbicides [D].Hangzhou:Zhejiang University,2015.
[2] Kumar A,Paietta J V.The sulfur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with-transducin repeats[J].Proc Natl Acad Sci USA,1995,92(8):3343-3347.doi:10.1073/pnas.92.8.3343.
[3] Jain M,Nijhawan A,Arora R,Agarwal P,Ray S,Sharma P,Kapoor S,Tyagi A K,Khurana J P.F-box proteins in rice.Genomewide analysis,classification,temporal and spatial gene expression during panicle and seed development,and regulation by light and abiotic stress [J].Plant Physiology,2007,143(4):1467-1483.doi:10.1104/pp.106.091900.
[4] Cui H R,Zhang Z R,Lv W,Xu J N,Wang X Y.Genome-wide characterization and analysis of f-box protein-encoding genes in the malusdomestica genome [J].Molecular Genetics & Genomics,2015,290(4):1435-1446.doi:10.1007/s00438-015-1004-z.
[5] Jia F,Wu B,Li H,Huang J G,Zheng C C.Genome-wide identification and characterisation of F-box family in maize [J].Molecular Genetics & Genomics,2013,288(11):559-577.doi:10.1007/s00438-013-0769-1.
[6] Koo A J,Thireault C,Zemelis S.Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis[J].Journal of Biological Chemistry,2014,289(43):29728.doi:10.1074/jbc.M114.603084.
[7] Sadanandom A,Bailey M,Ewan R,Lee J,Nelis S.The ubiquitin-proteasome system:Central modifier of plant signalling[J].New Phytol,2012,196:13-28.doi:10.1111/j.1469-8137.2012.04266.x.
[8] 吴丹,唐冬英,李新梅,李丽,赵小英,刘选明.F-box蛋白在植物生长发育中的功能研究进展[J].生命科学研究,2015,19(4):362-367.doi:10.16605/j.cnki.1007-7847.2015.04.012.Wu D,Tang D Y,Li X M,Li L,Zhao X Y,Liu X M.The research progress of F-box protein in plant growth and development [J].Life Science Research,2015,19(4):362-367.
[9] Liu Y,Ji X,Nie X.Arabidopsis AtbHLH112 regulates the expression of genes involved in abiotic stress tolerance by binding to their E-box and GCG-box motifs[J].New Phytologist,2015,207(3):692-709.doi:10.1111/nph.13387.
[10] Stone S L .The Role of Ubiquitin and the 26S proteasome in plant abiotic stress signaling[J].Frontiers in Plant Science,2014,5(5):135.doi:10.3389/fpls.2014.00135.
[11] Zhou S,Sun X,Yin S,Kong X,Zhou S,Xu Y.The role of the f-box gene tafba1 from wheat(Triticum aestivum L.)in drought tolerance[J].Plant Physiology and Biochemistry,2014,84:213-223.doi:10.1016/j.plaphy.2014.09.017.
[12] Zhang Y,Xu W,Li Z,Deng X,Wu W,Xue Y.F-Box protein DOR functions as a novel inhibitory factor for abscisic acid-induced stomatal closure under drought stress in Arabidopsis[J].Plant Physiology,2008,148(4):2121-2133.doi:10.1104/pp.108.126912.
[13] 许媛,李铃仙,于秀梅,刘大群.F-box蛋白在植物抗逆境胁迫中的功能[J].植物生理学报,2015,51(7):1003-1008.doi:10.13592/j.cnki.ppj.2015.0232.Xu Y,Lu L X,Yu X M,Liu D Q.The functions of F-box protein in plant resistance to stress[J].Journal of Plant Physiology,2015,51(7):1003-1008.
[14] Levin J Z,Meyerowitz E M.UFO:an Arabidopsis gene involved in both floral meristem and floral organ development[J].Plant Cell,1995,7:529-548.doi:10.1105/tpc.7.5.529.
[15] Hepworth S R,Haughn K G W .UFO in the Arabidopsis inflorescence apex is required for floral-meristem identity and bract suppression[J].Planta,2006,223(4):769-778.doi:10.2307/23389281.
[16] 霍冬英,郑炜君,李盼松,徐兆师,周永斌,陈明,马有志,闵东红,张小红.谷子F-box家族基因的鉴定、分类及干旱响应[J].作物学报,2014,40(9):1585-1594.doi:10.3724/SP.J.1006.2014.01585.Huo D Y,Zheng Y J,Li P S,Xu Z S,Zhou Y B,Chen M,Ma Y Z,Min D H,Zhang X H.Identification,classification and crought response of F-box family genes in millet[J].Acta Agronomica Sinica,2014,40(9):1585-1594.
[17] 于兰芳,周硕,张双喜,纪玉洁,刘永伟.葡萄F-box基因VvF-box5的基因结构与表达分析[J].植物遗传资源学报,2018,19(2):361-369.doi:10.13430/j.cnki.jpgr.2018.02.019.Yu L F,Zhou S,Zhang S G,Ji Y J,Liu Y W.Gene structure and expression analysis of grape F-box gene VvF-box5[J].Journal of Plant Genetic Resources,2018,19(2):361-369.
[18] 刘选明,孙孟思,李新梅,贺热情,屈丽娜,唐冬英,赵小英.F-box基因FOF2在拟南芥盐和冷胁迫响应中的功能分析[J].湖南大学学报(自然科学版),2018,45(6):120-127.doi:10.16339/j.cnki.hdxbzkb.2018.06.018.Liu X M,Sun M G,Li X M,He R Q,Qu L N,Tang D Y,Zhao X Y.Functional analysis of F-box gene FOF2 in Arabidopsis salt and cold stress response[J].Journal of Hunan University(Natural Science),2018,45(6):120-127.
[19] Wang Z,D L,Jiang Z,Peng W,Zhang L,Wang G,Xie D.GmCOI1,a soybean F-box protein gene,shows ability to mediate jasmonate-regulated plant defense and fertility in Arabidopsis[J].Mol Plant Microbe Interact,2005,18:1285-1295.doi:10.1094/MPMI-18-1285.
[20] Xue Z G,Zhang X M,Lei C F,Chen X J,Fu Y F.Molecular cloning and functional analysis of one ZEITLUPE homolog GmZTL3 in soybean[J].Mol Biol Rep,2012,39:1411-1418.doi:10.1007/s11033-011-0875-2.
[21] 倪志勇,王娟,吕萌,李波,白岩,范玲.棉花 4-香豆酸辅酶 A 连接酶基因克隆及原核表达[J].西北植物学报,2010,30(3):429-436.doi:1000-4025(2010)03-0429-08.Ni Z Y,Wang J,Lü M,Li B,Bai Y,Fan L.Cloning and prokaryotic expression of cotton 4-coumaric acid coenzyme A ligase gene[J].Acta Botanica Sinica,2010,30(3):429-436.
[22] Somers D E,Fujiwara S.Thinking outside the F-box:Novel ligands for novel receptors[J].Trends Plant Sci,2009,14:206-213.doi:10.1016/j.tplants.2009.01.003.
[23] Lechner E,Achard P,Vansiri A,Potuschak T,Genschik P.F-box proteins everywhere[J].Curr Opin Plant Biol,2006,9:631-638.
[24] Jeevan R J,Vignesh D,Parameswari P,Prasath S D,Ryun S C,Young S Y,Hong S,Heon S O,Ho M O,Pyo Y L.F-Box genes in brassica rapa:genome-wide identification,structural characterization,expressional validation,and comparative analysis[J].Plant Molecular Biology Reporter,2018,36(3):500-517.doi:10.1007/s11105-018-1083-1.
[25] Jia Q,Xiao Z X,Wong F L,Sun S,Liang K J,Lam H M.Genome-wide analyses of the soybean F-box gene family in response to salt stress[J].Int J Mol Sci,2017,18(4):818.doi:10.3390/ijms18040818.
[26] Bellieny-Rabelo D,Oliveira A E A,Venancio T M.Impact of whole-genome and tandem duplications in the expansion and functional diversification of the F-box family in legumes(Fabaceae)[J].PLoS One,2013,8(2):e55127.doi:10.1371/journal.pone.0055127.
[27] 陈秀秀,张彤,余倩文,周薇,安逸民,杜秉昊,郭长虹.紫花苜蓿F-box蛋白基因MsFTL的克隆及功能分析[J].植物遗传资源学报,2019,20(3):750-759.doi:10.13430/j.cnki.jpgr.20180929001.Chen X X,Zhang W,Yu Q W,Zhou W,An Y M,Du B Z,Guo C H.Cloning and functional analysis of F-box pr-otein gene MsFTL in alfalfa[J].Journal of Plant Genetic Resources,2019,20(3):750-759.
[28] Chen Z H,Li M T,Yuan Y.Ectopic expression of cucumber(Cucumis sativus L.)Cs TIR/AFB genes enhance salt tolerance in transgenic Arabidopsis[J].Plant Cell Tissue and Organ Culture,2017,131(1):107-118.doi:10.1007/s11240-017-1267-7.
[29] An J P,Li R,Qu F J.Apple F-Box protein Md MAX2 regulates plant photomorphogenesis and stress response[J].Frontiers in Plant Science,2016,7(1235):1685-1699.doi:10.3389/fpls.2016.01685.
[30] Lyzenga W J,Stone S L.Abiotic stress tolerance mediated by protein ubiquitinnation[J].J Exp Bot,2012;63:599-616.doi:10.1093/jxb/err310.
[31] Xu G,Cui Y,Wang M.OsMsr9,a novel putative rice F-box containing protein,confers enhanced salt tolerance in transgenic rice and Arabidopsis[J].Molecular Breeding,2014,34(3):1055-1064.doi:10.1007/s11032-014-0096-1.
[32] Gupta S,Garg V,Kant C,Bhatia S.Genome-wide survey and expression analysis of F-box genes in chickpea[J].Bmc Genomics,2015,16(1):67.doi:10.1186/s12864-015-1293-y.
[33] Kim Y Y,Cui M H,Noh M S,Jung K W,Shin J S.The FBA motif-containing protein AFBA1 acts as a novel positive regulator of ABA response in Arabidopsis[J].Plant and Cell Physiology,2017,58(3):574-586.doi:10.1093/pcp/pcx003.
[34] Calderón-Villalobos L I A,Nill C,Marrocco K,Kretsch T,Schwechheimer C.The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is required for efficient translation during temperature stress[J].Gene,2007,392:106-116.doi:10.1016/j.gene.2006.11.016.
[35] Jia Y Y,Gu H Y,Wang X S,Chen Q J,Shi S B,Zhang J S,Ma L,Zhang H,Ma H.Molecular cloning and characterization of an F-box family gene Carf-box1 from chickpea(Cicer arietinuml L.)[J].Molecular Biology Reports,2012,39(3):2337-2345.doi:10.1007/s11033-011-0984-y.
[36] Yan Y S,Chen X Y,Yang K,Sun Z X,Fu Y P,Zhang Y M,Fang R X.Overexpression of an F-box protein gene reduces abiotic stress tolerance and promotes root growth in rice[J].Molecular Plant,2011,4(1):190-197.doi:10.1093/mp/ssq066.
[37] Guerra D,Mastrangelo A M,Lopez-Torrejon G,Marzin S,Schweizer P,Stanca A M,del Pozo J C,Cattivelli L,Mazzucotelli E.Identification of a protein network interacting with TdRF1,a wheat RING ubiquitin ligase with a protective role against cellular dehydration[J].Plant Physiol,2012,158(2):777-789.doi:10.2307/41435407 .
[38] Gusti A,Baumberger N,Nowack M,Pusch S,Eisler H,Potuschak T,De Veylder L,Schnittger A,Genschik P.The Arabidopsis thaliana F-box protein FBL17 is essential for progression through the second mitosis during pollen development[J].PLoS One,2009,4:e4780.doi:10.1371/journal.pone.0004780.
[39] Mito T,Seki M,Shinozaki K,Ohme-Takagi M,Matsui K.Generation of chimeric repressors that confer salt tolerance in arabidopsis and rice[J].Plant Biotechnology Journal,2011,9(7):736-746.doi:10.1111/j.1467-7652.2010.00578.x.
[2] Kumar A,Paietta J V.The sulfur controller-2 negative regulatory gene of Neurospora crassa encodes a protein with-transducin repeats[J].Proc Natl Acad Sci USA,1995,92(8):3343-3347.doi:10.1073/pnas.92.8.3343.
[3] Jain M,Nijhawan A,Arora R,Agarwal P,Ray S,Sharma P,Kapoor S,Tyagi A K,Khurana J P.F-box proteins in rice.Genomewide analysis,classification,temporal and spatial gene expression during panicle and seed development,and regulation by light and abiotic stress [J].Plant Physiology,2007,143(4):1467-1483.doi:10.1104/pp.106.091900.
[4] Cui H R,Zhang Z R,Lv W,Xu J N,Wang X Y.Genome-wide characterization and analysis of f-box protein-encoding genes in the malusdomestica genome [J].Molecular Genetics & Genomics,2015,290(4):1435-1446.doi:10.1007/s00438-015-1004-z.
[5] Jia F,Wu B,Li H,Huang J G,Zheng C C.Genome-wide identification and characterisation of F-box family in maize [J].Molecular Genetics & Genomics,2013,288(11):559-577.doi:10.1007/s00438-013-0769-1.
[6] Koo A J,Thireault C,Zemelis S.Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis[J].Journal of Biological Chemistry,2014,289(43):29728.doi:10.1074/jbc.M114.603084.
[7] Sadanandom A,Bailey M,Ewan R,Lee J,Nelis S.The ubiquitin-proteasome system:Central modifier of plant signalling[J].New Phytol,2012,196:13-28.doi:10.1111/j.1469-8137.2012.04266.x.
[8] 吴丹,唐冬英,李新梅,李丽,赵小英,刘选明.F-box蛋白在植物生长发育中的功能研究进展[J].生命科学研究,2015,19(4):362-367.doi:10.16605/j.cnki.1007-7847.2015.04.012.Wu D,Tang D Y,Li X M,Li L,Zhao X Y,Liu X M.The research progress of F-box protein in plant growth and development [J].Life Science Research,2015,19(4):362-367.
[9] Liu Y,Ji X,Nie X.Arabidopsis AtbHLH112 regulates the expression of genes involved in abiotic stress tolerance by binding to their E-box and GCG-box motifs[J].New Phytologist,2015,207(3):692-709.doi:10.1111/nph.13387.
[10] Stone S L .The Role of Ubiquitin and the 26S proteasome in plant abiotic stress signaling[J].Frontiers in Plant Science,2014,5(5):135.doi:10.3389/fpls.2014.00135.
[11] Zhou S,Sun X,Yin S,Kong X,Zhou S,Xu Y.The role of the f-box gene tafba1 from wheat(Triticum aestivum L.)in drought tolerance[J].Plant Physiology and Biochemistry,2014,84:213-223.doi:10.1016/j.plaphy.2014.09.017.
[12] Zhang Y,Xu W,Li Z,Deng X,Wu W,Xue Y.F-Box protein DOR functions as a novel inhibitory factor for abscisic acid-induced stomatal closure under drought stress in Arabidopsis[J].Plant Physiology,2008,148(4):2121-2133.doi:10.1104/pp.108.126912.
[13] 许媛,李铃仙,于秀梅,刘大群.F-box蛋白在植物抗逆境胁迫中的功能[J].植物生理学报,2015,51(7):1003-1008.doi:10.13592/j.cnki.ppj.2015.0232.Xu Y,Lu L X,Yu X M,Liu D Q.The functions of F-box protein in plant resistance to stress[J].Journal of Plant Physiology,2015,51(7):1003-1008.
[14] Levin J Z,Meyerowitz E M.UFO:an Arabidopsis gene involved in both floral meristem and floral organ development[J].Plant Cell,1995,7:529-548.doi:10.1105/tpc.7.5.529.
[15] Hepworth S R,Haughn K G W .UFO in the Arabidopsis inflorescence apex is required for floral-meristem identity and bract suppression[J].Planta,2006,223(4):769-778.doi:10.2307/23389281.
[16] 霍冬英,郑炜君,李盼松,徐兆师,周永斌,陈明,马有志,闵东红,张小红.谷子F-box家族基因的鉴定、分类及干旱响应[J].作物学报,2014,40(9):1585-1594.doi:10.3724/SP.J.1006.2014.01585.Huo D Y,Zheng Y J,Li P S,Xu Z S,Zhou Y B,Chen M,Ma Y Z,Min D H,Zhang X H.Identification,classification and crought response of F-box family genes in millet[J].Acta Agronomica Sinica,2014,40(9):1585-1594.
[17] 于兰芳,周硕,张双喜,纪玉洁,刘永伟.葡萄F-box基因VvF-box5的基因结构与表达分析[J].植物遗传资源学报,2018,19(2):361-369.doi:10.13430/j.cnki.jpgr.2018.02.019.Yu L F,Zhou S,Zhang S G,Ji Y J,Liu Y W.Gene structure and expression analysis of grape F-box gene VvF-box5[J].Journal of Plant Genetic Resources,2018,19(2):361-369.
[18] 刘选明,孙孟思,李新梅,贺热情,屈丽娜,唐冬英,赵小英.F-box基因FOF2在拟南芥盐和冷胁迫响应中的功能分析[J].湖南大学学报(自然科学版),2018,45(6):120-127.doi:10.16339/j.cnki.hdxbzkb.2018.06.018.Liu X M,Sun M G,Li X M,He R Q,Qu L N,Tang D Y,Zhao X Y.Functional analysis of F-box gene FOF2 in Arabidopsis salt and cold stress response[J].Journal of Hunan University(Natural Science),2018,45(6):120-127.
[19] Wang Z,D L,Jiang Z,Peng W,Zhang L,Wang G,Xie D.GmCOI1,a soybean F-box protein gene,shows ability to mediate jasmonate-regulated plant defense and fertility in Arabidopsis[J].Mol Plant Microbe Interact,2005,18:1285-1295.doi:10.1094/MPMI-18-1285.
[20] Xue Z G,Zhang X M,Lei C F,Chen X J,Fu Y F.Molecular cloning and functional analysis of one ZEITLUPE homolog GmZTL3 in soybean[J].Mol Biol Rep,2012,39:1411-1418.doi:10.1007/s11033-011-0875-2.
[21] 倪志勇,王娟,吕萌,李波,白岩,范玲.棉花 4-香豆酸辅酶 A 连接酶基因克隆及原核表达[J].西北植物学报,2010,30(3):429-436.doi:1000-4025(2010)03-0429-08.Ni Z Y,Wang J,Lü M,Li B,Bai Y,Fan L.Cloning and prokaryotic expression of cotton 4-coumaric acid coenzyme A ligase gene[J].Acta Botanica Sinica,2010,30(3):429-436.
[22] Somers D E,Fujiwara S.Thinking outside the F-box:Novel ligands for novel receptors[J].Trends Plant Sci,2009,14:206-213.doi:10.1016/j.tplants.2009.01.003.
[23] Lechner E,Achard P,Vansiri A,Potuschak T,Genschik P.F-box proteins everywhere[J].Curr Opin Plant Biol,2006,9:631-638.
[24] Jeevan R J,Vignesh D,Parameswari P,Prasath S D,Ryun S C,Young S Y,Hong S,Heon S O,Ho M O,Pyo Y L.F-Box genes in brassica rapa:genome-wide identification,structural characterization,expressional validation,and comparative analysis[J].Plant Molecular Biology Reporter,2018,36(3):500-517.doi:10.1007/s11105-018-1083-1.
[25] Jia Q,Xiao Z X,Wong F L,Sun S,Liang K J,Lam H M.Genome-wide analyses of the soybean F-box gene family in response to salt stress[J].Int J Mol Sci,2017,18(4):818.doi:10.3390/ijms18040818.
[26] Bellieny-Rabelo D,Oliveira A E A,Venancio T M.Impact of whole-genome and tandem duplications in the expansion and functional diversification of the F-box family in legumes(Fabaceae)[J].PLoS One,2013,8(2):e55127.doi:10.1371/journal.pone.0055127.
[27] 陈秀秀,张彤,余倩文,周薇,安逸民,杜秉昊,郭长虹.紫花苜蓿F-box蛋白基因MsFTL的克隆及功能分析[J].植物遗传资源学报,2019,20(3):750-759.doi:10.13430/j.cnki.jpgr.20180929001.Chen X X,Zhang W,Yu Q W,Zhou W,An Y M,Du B Z,Guo C H.Cloning and functional analysis of F-box pr-otein gene MsFTL in alfalfa[J].Journal of Plant Genetic Resources,2019,20(3):750-759.
[28] Chen Z H,Li M T,Yuan Y.Ectopic expression of cucumber(Cucumis sativus L.)Cs TIR/AFB genes enhance salt tolerance in transgenic Arabidopsis[J].Plant Cell Tissue and Organ Culture,2017,131(1):107-118.doi:10.1007/s11240-017-1267-7.
[29] An J P,Li R,Qu F J.Apple F-Box protein Md MAX2 regulates plant photomorphogenesis and stress response[J].Frontiers in Plant Science,2016,7(1235):1685-1699.doi:10.3389/fpls.2016.01685.
[30] Lyzenga W J,Stone S L.Abiotic stress tolerance mediated by protein ubiquitinnation[J].J Exp Bot,2012;63:599-616.doi:10.1093/jxb/err310.
[31] Xu G,Cui Y,Wang M.OsMsr9,a novel putative rice F-box containing protein,confers enhanced salt tolerance in transgenic rice and Arabidopsis[J].Molecular Breeding,2014,34(3):1055-1064.doi:10.1007/s11032-014-0096-1.
[32] Gupta S,Garg V,Kant C,Bhatia S.Genome-wide survey and expression analysis of F-box genes in chickpea[J].Bmc Genomics,2015,16(1):67.doi:10.1186/s12864-015-1293-y.
[33] Kim Y Y,Cui M H,Noh M S,Jung K W,Shin J S.The FBA motif-containing protein AFBA1 acts as a novel positive regulator of ABA response in Arabidopsis[J].Plant and Cell Physiology,2017,58(3):574-586.doi:10.1093/pcp/pcx003.
[34] Calderón-Villalobos L I A,Nill C,Marrocco K,Kretsch T,Schwechheimer C.The evolutionarily conserved Arabidopsis thaliana F-box protein AtFBP7 is required for efficient translation during temperature stress[J].Gene,2007,392:106-116.doi:10.1016/j.gene.2006.11.016.
[35] Jia Y Y,Gu H Y,Wang X S,Chen Q J,Shi S B,Zhang J S,Ma L,Zhang H,Ma H.Molecular cloning and characterization of an F-box family gene Carf-box1 from chickpea(Cicer arietinuml L.)[J].Molecular Biology Reports,2012,39(3):2337-2345.doi:10.1007/s11033-011-0984-y.
[36] Yan Y S,Chen X Y,Yang K,Sun Z X,Fu Y P,Zhang Y M,Fang R X.Overexpression of an F-box protein gene reduces abiotic stress tolerance and promotes root growth in rice[J].Molecular Plant,2011,4(1):190-197.doi:10.1093/mp/ssq066.
[37] Guerra D,Mastrangelo A M,Lopez-Torrejon G,Marzin S,Schweizer P,Stanca A M,del Pozo J C,Cattivelli L,Mazzucotelli E.Identification of a protein network interacting with TdRF1,a wheat RING ubiquitin ligase with a protective role against cellular dehydration[J].Plant Physiol,2012,158(2):777-789.doi:10.2307/41435407 .
[38] Gusti A,Baumberger N,Nowack M,Pusch S,Eisler H,Potuschak T,De Veylder L,Schnittger A,Genschik P.The Arabidopsis thaliana F-box protein FBL17 is essential for progression through the second mitosis during pollen development[J].PLoS One,2009,4:e4780.doi:10.1371/journal.pone.0004780.
[39] Mito T,Seki M,Shinozaki K,Ohme-Takagi M,Matsui K.Generation of chimeric repressors that confer salt tolerance in arabidopsis and rice[J].Plant Biotechnology Journal,2011,9(7):736-746.doi:10.1111/j.1467-7652.2010.00578.x.