一种黑木耳酸性多糖的分离纯化及其结构鉴定
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摘要
采用水提醇沉法从黑木耳粉中提取黑木耳多糖。通过离子交换纤维素(Diethylaminoethyl-52,DEAE-52)及葡聚糖凝胶层析Sephadex G-100对黑木耳子实体多糖(Auricularia auricula polysaccharide,AAP)进行纯化,采用红外光谱(infrared spectroscopy,FT-IR)、高效液相色谱(high performance liquid chromatography,HPLC)、气质联用(gas chromatography-mass spectrometry,GC-MS)技术对其主要组分的理化性质和化学结构进行分析,结果表明:AAP为一种具有高分支结构的葡聚糖,黏均分子量为4.50×10~3 Da,其所含鼠李糖、葡萄糖、木糖、甘露糖和半乳糖的含量比分别为0.3%、17.1%、0.3%、3.1%和63.2%,通过进一步对AAP甲基化分析,并对其糖残基的连接方式进行分析,AAP含有→6)-α-D-Galp-(1→、-Manp-(1→、→3,4)-α-D-Manp-(1→、→3)-α-D-Manp-(1→和→6)-α-D-Glcp-(1→等残基。
Auricularia auricula polysaccharide(AAP) was extracted from Auricularia auricula powder with extraction and alcohol precipitation,and then purified using ion exchange cellulose(DEAE-52)and Sephadex gel chromatography(Sephadex G-100),the physicochemical properties and chemical structures of main components were characterized using the gas chromatography-mass spectrometry(GC-MS),infrared spectroscopy(FT-IR),high performance liquid chromatography(HPLC). The results showed that AAP was a high branched glucan,the viscosity average molecular weight was 4.50 × 10~3 Da,the percent of rhamnose,glucose,xylose,mannose and galactose was 0.3 %,17.1 %,0.3 %,3.1 %,and 63.2 %,respectively,the methylation analysis were used to analyse the sugar residue of AAP,the result showed that AAP contained →6)-α-D-Galp-(1→,-Manp-(1→,→3,4)-α-D-Manp-(1→,→3)-α-D-Manp-(1→ and →6)-α-DGlcp-(1→.
Auricularia auricula polysaccharide(AAP) was extracted from Auricularia auricula powder with extraction and alcohol precipitation,and then purified using ion exchange cellulose(DEAE-52)and Sephadex gel chromatography(Sephadex G-100),the physicochemical properties and chemical structures of main components were characterized using the gas chromatography-mass spectrometry(GC-MS),infrared spectroscopy(FT-IR),high performance liquid chromatography(HPLC). The results showed that AAP was a high branched glucan,the viscosity average molecular weight was 4.50 × 10~3 Da,the percent of rhamnose,glucose,xylose,mannose and galactose was 0.3 %,17.1 %,0.3 %,3.1 %,and 63.2 %,respectively,the methylation analysis were used to analyse the sugar residue of AAP,the result showed that AAP contained →6)-α-D-Galp-(1→,-Manp-(1→,→3,4)-α-D-Manp-(1→,→3)-α-D-Manp-(1→ and →6)-α-DGlcp-(1→.
引文
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[2]李兴泰,高明波.黑木耳多糖清除活性氧及保护线粒体[J].食品科学,2004,25(1):171-173
[3]Fan L S,Zhang S H,Yu L,et al.Evaluation of Antioxidant Property and Quality of Breads Containing Auricularia Auricula Polysaccharide Flour[J].Food Chemistry,2007,101(3):1158-1163
[4]Acharya K,Samui K,Rai M,et al.Antioxidant and Nitric Oxide Synthase Activation Properties of Auricularia Auricula[J].Indian Journal of Experimental Biology,2004,42(5):538-540
[5]Takeuchi H,He P,Mooi L Y.Reductive Effect of Hot-water Extracts from Woody Ear(Auricularia auricula judae Quel.)on Food Intake and Blood Glucose Concentration in Genetically Diabetic KK-Ay Mice[J].Journal of Nutritional Science and Vitaminology,2004,50(4):300-304
[6]Yoon S J,Yu M A,Pyun Y R,et al.The Nontoxic Mushroom Auricularia Auricula Contains a Polysaccharide with Anticoagulant Activity Mediated by Antithrombin[J].Thrombosis Research,2003,112(3):151-158
[7]Ma Z C,Wang J G,Zhang L N,et al.Evaluation of Water Soluble β-D-glucan from Auricularia Auricular judae as Potential Anti-tumor Agent[J].Carbohydrate Polymers,2010,80:977-983
[8]Song G L,Du Q Z.Structure Characterization and Antitumor Activity of anαβ-glucan Polysaccharide from Auricularia Polytricha[J].Food Research International,2012,45:381-387
[9]张会新,刘洪雨,刘畅.黑木耳多糖对小鼠免疫功能的影响[J].动物医学进展,2009,30(7):23-25
[10]邱军强,张华,刘迪迪.9种食用菌碱水提取物的抗氧化活性比较研究[J].食品研究与开发,2017,38(15):1-5
[11]Song G L,Du Q Z.Structure Characterization and Antitumor Activity of an α β-glucan Polysaccharide from Auricularia Polytricha[J].Food Research International,2012,45:381-387
[12]Zeng W C,Zhang Z,Gao H,et al.Characterization of Antioxidant Polysaccharides from Auricularia Auricular Using Microwave-assisted Extraction[J].Carbohydrate Polymers,2012,89:694-700
[13]Zhang J,Wang H Y,Zheng Q S.Cardioprotective Effect of Aralia E-lata Polysaccharide on Myocardial Ischemic Reperfusion(IR)Injury in Rats[J].International Journal of Biological Macromolecules,2013,59:328-332
[14]彭辉.聚酯的特性黏度与聚合度[J].聚酯工业,2004,17(3):3-4
[15]薛娟.关黄柏多糖分离、纯化与免疫活性研究[D].哈尔滨:黑龙江中医药大学,2010:29-40
[16]张华.黑木耳中性多糖片段硫酸酯对辐射诱导氧化应激防护作用[D].哈尔滨:哈尔滨工业大学,2013:41-60
[17]孙延平.牵牛子的性味研究[D].哈尔滨:黑龙江中医药大学,2014:129-172
[18]白海娜.黑木耳多糖与原花青素60Co辐射诱导氧化损伤的协同防护作用[D].哈尔滨:哈尔滨工业大学,2016:62-69