6种人参属药材体外抗凝血活性与皂苷含量的相关性研究
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摘要
目的分析不同功效的6种人参属药材提取物的体外凝抗血活性差异。方法采用凝血酶原时间(PT)、活化部分凝血酶原时间(APTT)、纤维蛋白原(FIB)实验,探讨6种人参属药材提取物抗凝血活性的差异性;分析其抗凝血活性与皂苷含量的相关性。结果竹节参、珠子参及三七可显著延长PT、APTT,降低FIB水平,呈现明显的量效关系;而人参、西洋参及屏边三七无显著变化。竹节参、珠子参提取物的抗凝血活性明显强于三七提取物。6种人参属药材中人参皂苷含量与其抗凝血活性的相关性分析表明,PT与人参皂苷Rb2含量呈现显著的正相关;且人参皂苷Rb2可显著延长PT、APTT。结论 6种人参属药材抗凝血活性存在差异,且抗凝血活性与人参皂苷Rb2含量相关。
Objective To analyze the effects of different extracts from six Panax species on coagulation properties in vitro. Methods The prothrombin time(PT), activated partial thromboplastin time(APTT), fibrinogen(FIB) assays were used to investigate the difference of anticoagulant activity of extracts and the correlation between anticoagulant activity and saponin content in six Panax species. Results The results showed that Panax japonicus, P. japlcus, and P. notoginseng significantly prolonged PT and APTT, and reduced FIB content in a dose-dependent manner, while P. ginseng, P. quinquefolius, and P. stipuleanatus had no significant effects. Compared with the P. notoginseng group, the anticoagulant activity of the extracts of P. japonicus and P. japlcus was significantly stronger than that of P. notoginseng. Correlation analysis of ginsenoside content and anticoagulant activity showed that PT presented a positive correlation with ginsenoside Rb2 significantly. Ginsenoside Rb2 significantly prolonged PT and APTT. Conclusion In summary, and anticoagulant activity is related to ginsenoside Rb2 content.
Objective To analyze the effects of different extracts from six Panax species on coagulation properties in vitro. Methods The prothrombin time(PT), activated partial thromboplastin time(APTT), fibrinogen(FIB) assays were used to investigate the difference of anticoagulant activity of extracts and the correlation between anticoagulant activity and saponin content in six Panax species. Results The results showed that Panax japonicus, P. japlcus, and P. notoginseng significantly prolonged PT and APTT, and reduced FIB content in a dose-dependent manner, while P. ginseng, P. quinquefolius, and P. stipuleanatus had no significant effects. Compared with the P. notoginseng group, the anticoagulant activity of the extracts of P. japonicus and P. japlcus was significantly stronger than that of P. notoginseng. Correlation analysis of ginsenoside content and anticoagulant activity showed that PT presented a positive correlation with ginsenoside Rb2 significantly. Ginsenoside Rb2 significantly prolonged PT and APTT. Conclusion In summary, and anticoagulant activity is related to ginsenoside Rb2 content.
引文
[1]李旭霞,李庆伟.抗凝血蛋白药物的研究进展[J].辽宁师范大学学报:自然科学版,2003(2):187-191.
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[3]Krichen F,Bougatef H,Sayari N,et al.Isolation,purification and structural characterestics of chondroitin sulfate from smooth hound cartilage:In vitro anticoagulant and antiproliferative properties[J].Carbohyd Polym,2018,doi:10.1016/j.carbpol.2018.06.040.
[4]Singh D,Chaudhuri P K.Structural characteristics,bioavailability and cardioprotective potential of saponins[J].Integr Med Res,2018,7(1):33-43.
[5]路放,杨世海,孟宪兰.人参药理作用研究新进展[J].人参研究,2013,25(1):46-52.
[6]王莹,禇扬,李伟,等.三七中皂苷成分及其药理作用的研究进展[J].中草药,2015,46(9):1381-1392.
[7]尚金燕,李桂荣,邵明辉,等.西洋参的药理作用研究进展[J].人参研究,2016,28(6):49-51.
[8]包旭,詹常森,肖倬殷.近十年来珠子参的皂甙成分及药理研究进展[J].华西药学杂志,1992,7(1):49-52.
[9]欧阳丽娜,向大位,吴雪,等.竹节参化学成分及药理活性研究进展[J].中草药,2010,41(6):1023-1027.
[10]Liang C,Ding Y,Song S B,et al.Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-kappa B[J].J Gins Res,2013,37(1):74-79.
[11]Yang B R,Yuen S C,Fan G Y,et al.Identification of certain Panax species to be potential substitutes for Panax notoginseng in hemostatic treatments[J].Pharmacol Res,2018,doi:10.1016/j.phrs.2018.05.005.
[12]李长田,王海波,贾传文.人参、西洋参和三七提取物抗凝血活性的研究[J].长春中医药大学学报,2011,27(2):169-170.
[13]Huang X,Liu Y,Zhang N,et al.UPLC Orbitrap HRMSanalysis of Panax quinquefolium L.for authentication of Panax genus with chemometric methods[J].JChromatogr Sci,2018,56(1),25-35.
[14]张晓旭,马雪涛,胡蒙,等.超高效液相色谱法检测6种人参皂苷含量[J].中国食品学报,2015,15(5):241-246.
[15]杨崇仁,姜志东,周俊,等.屏边三七根茎中的两个新的齐墩果酸皂甙[J].云南植物研究,1985,7(1):103-108.
[16]贾放,陈平,王如锋,等.竹节参药材不同部位皂苷成分的分析研究[J].时珍国医国药,2012,23(7):1682-1684.
[17]宋小妹,李林,杨光明,等.不同产地珠子参中竹节参皂苷IVa含量测定[J].中国中药杂志,2010,35(7):885-887.
[18]赵玉珑,范开,刘岩,等.三七不同部位提取物抗凝血活性的研究[J].西北植物学报,2013,33(3):626-631.
[19]张英杰,王会君,侯荣伟,等.凝血四项的临床应用[J].检验医学与临床,2013,10(4):450-452.
[2]徐恒启,陈克利.国内抗凝血、抗血栓多糖的药理研究进展[J].生物学杂志,2005,22(1):14-16.
[3]Krichen F,Bougatef H,Sayari N,et al.Isolation,purification and structural characterestics of chondroitin sulfate from smooth hound cartilage:In vitro anticoagulant and antiproliferative properties[J].Carbohyd Polym,2018,doi:10.1016/j.carbpol.2018.06.040.
[4]Singh D,Chaudhuri P K.Structural characteristics,bioavailability and cardioprotective potential of saponins[J].Integr Med Res,2018,7(1):33-43.
[5]路放,杨世海,孟宪兰.人参药理作用研究新进展[J].人参研究,2013,25(1):46-52.
[6]王莹,禇扬,李伟,等.三七中皂苷成分及其药理作用的研究进展[J].中草药,2015,46(9):1381-1392.
[7]尚金燕,李桂荣,邵明辉,等.西洋参的药理作用研究进展[J].人参研究,2016,28(6):49-51.
[8]包旭,詹常森,肖倬殷.近十年来珠子参的皂甙成分及药理研究进展[J].华西药学杂志,1992,7(1):49-52.
[9]欧阳丽娜,向大位,吴雪,等.竹节参化学成分及药理活性研究进展[J].中草药,2010,41(6):1023-1027.
[10]Liang C,Ding Y,Song S B,et al.Oleanane-triterpenoids from Panax stipuleanatus inhibit NF-kappa B[J].J Gins Res,2013,37(1):74-79.
[11]Yang B R,Yuen S C,Fan G Y,et al.Identification of certain Panax species to be potential substitutes for Panax notoginseng in hemostatic treatments[J].Pharmacol Res,2018,doi:10.1016/j.phrs.2018.05.005.
[12]李长田,王海波,贾传文.人参、西洋参和三七提取物抗凝血活性的研究[J].长春中医药大学学报,2011,27(2):169-170.
[13]Huang X,Liu Y,Zhang N,et al.UPLC Orbitrap HRMSanalysis of Panax quinquefolium L.for authentication of Panax genus with chemometric methods[J].JChromatogr Sci,2018,56(1),25-35.
[14]张晓旭,马雪涛,胡蒙,等.超高效液相色谱法检测6种人参皂苷含量[J].中国食品学报,2015,15(5):241-246.
[15]杨崇仁,姜志东,周俊,等.屏边三七根茎中的两个新的齐墩果酸皂甙[J].云南植物研究,1985,7(1):103-108.
[16]贾放,陈平,王如锋,等.竹节参药材不同部位皂苷成分的分析研究[J].时珍国医国药,2012,23(7):1682-1684.
[17]宋小妹,李林,杨光明,等.不同产地珠子参中竹节参皂苷IVa含量测定[J].中国中药杂志,2010,35(7):885-887.
[18]赵玉珑,范开,刘岩,等.三七不同部位提取物抗凝血活性的研究[J].西北植物学报,2013,33(3):626-631.
[19]张英杰,王会君,侯荣伟,等.凝血四项的临床应用[J].检验医学与临床,2013,10(4):450-452.