游泳池水引起的聚集性感染人腺病毒基因特征分析
|
摘要
目的了解淮安市一游泳池聚集性感染人腺病毒的hexon基因特征。方法采用实时荧光定量RT-PCR方法对12例游泳后出现呼吸道感染患儿咽拭子标本和2份游泳池水样进行呼吸道多病原体的快速筛查,对核酸阳性的标本进行hexon基因的部分序列扩增、测序并构建系统发生树,分析hexon基因特征及人腺病毒遗传多样性确定腺病毒的型别。结果 12例患儿中有9份咽拭子标本和1份儿童池游泳水样扩增出人腺病毒hexon基因片段,测序分析该毒株(淮安株)与我国GZ01株、日本J1007、美国株(USA27440、USA 5497、USA NHR3)核苷酸同源性及推导的氨基酸同源性均为99.9%。系统进化树分析显示,淮安株属于人腺病毒E亚属4基因型的进化谱系枝。结论该起聚集性呼吸道感染疫情可能由E亚属4基因型人腺病毒污染游泳池水引起。
Objective To study the genetic characteristics of adenovirus from clustered human cases occurring at a municipal swimming pool. Methods Nasopharyngeal swabs were collected from 12 pediatric patients who developed an upper respiratory tract infection after swimming, and 2 samples of pool water were collected. Samples were screened for multiple respiratory pathogens using quantitative real-time RT-PCR. The genetic characteristics of and the phylogenetic relationship among human adenoviruses were determined by analyzing the sequence of the hexon gene. Results Human adenovirus nucleic acids were detected in 9 of 12 nasopharyngeal swabs and one sample of pool water. Comparison indicated that the Huai'an strains were very closely related to GZ01, J1007, USA27440, USA 5497, USA NHR3. Nucleotide sequences were 99.9% similar, and deduced amino acid sequences were 99.9% similar. A phylogenetic tree indicated the Huai'an strains clustered together and belonged to a genetic lineage of HAdV-4 within the E species. Conclusion Swimming pool water contaminated with HAdV-4 was the most likely source of infection.
Objective To study the genetic characteristics of adenovirus from clustered human cases occurring at a municipal swimming pool. Methods Nasopharyngeal swabs were collected from 12 pediatric patients who developed an upper respiratory tract infection after swimming, and 2 samples of pool water were collected. Samples were screened for multiple respiratory pathogens using quantitative real-time RT-PCR. The genetic characteristics of and the phylogenetic relationship among human adenoviruses were determined by analyzing the sequence of the hexon gene. Results Human adenovirus nucleic acids were detected in 9 of 12 nasopharyngeal swabs and one sample of pool water. Comparison indicated that the Huai'an strains were very closely related to GZ01, J1007, USA27440, USA 5497, USA NHR3. Nucleotide sequences were 99.9% similar, and deduced amino acid sequences were 99.9% similar. A phylogenetic tree indicated the Huai'an strains clustered together and belonged to a genetic lineage of HAdV-4 within the E species. Conclusion Swimming pool water contaminated with HAdV-4 was the most likely source of infection.
引文
[1] Rowe WP,Huebner RJ,Gilmore LK,et al.Isolation of a cytopathogenic agent from human adenoids undergoing spontaneous degeneration in tissue Culture[J].Proc Soc Exp Biol Med,1953,84(3):570-3.
[2] 高文娟,金玉,段招军.人腺病毒的研究进展[J].病毒学报,2014,30(2):193-200.
[3] Ghebremedhin B.Human adenovirus:Viral pathogen with increasingimportance[J].Eur J Microbiol Immunol,2014,4(1):26-33.
[4] Ma Q,Tian X,Jiang Z,et al.Neutralizing epitopes mapping of human adenovirus type 14 hexon[J].Vaccine,2015,33(48):6659-65.
[5] Klein SR,Jiang H,Hossain MB,et al.Critical role of autophagy in the processing of adenovirus Capsid-Incorporated Cancer-Specific antigens[J].Plos One,2016,11(4):e0153814.
[6] Hilleman MR,Werner JH.Recovery of new agent from patients with acute respiratory illness[J].Proc Soc Exp Biol Med,1954,85(1):183-8.
[7] 黄国虹,许文波.腺病毒新型别的研究进展[J].病毒学报,2013,29(3):342-8.
[8] 田新贵,周荣.人腺病毒分型及分子进化研究进展[J].中华微生物学和免疫学杂志,2014(6):481-6.
[9] Tong HI,Lu Y.Effective detection of human adenovirus in hawaiian waters using enhanced pcr methods[J].Virol J,2011,8(1):1-6.
[10] 王媛媛,裴广倩,赵飞扬,等.腺病毒41型感染致儿童腹泻一例临床表现及病毒基因组学特征分析[J].中国病原生物学杂志,2018,13(11):1236-42.
[11] Chen M,Zhu Z,Huang F,et al.Adenoviruses associated with acute respiratory diseases reported in Beijing from 2011 to,2013[J].PLoS One,2015(10):e0121375.
[12] Echavarria M,Sanchez JL,Kolavic-Gray SA,et al.Rapid detection of adenovirus in throat swab specimens by PCR during respiratory disease outbreaks among military recruits[J].J Clin Microbiol,2003,41(2):810-2.
[13] Zhu Z,Zhang Y,Xu S,et al.Outbreak of acute respiratory disease in China caused by B2 species of adenovirus type 11[J].J Clin Microbiol,2009,47(3):697-703.
[14] D'Angelo LJ,Hierholzer JC,Keenlyside RA,et al.Pharyngoconjunctival fever caused by adenovirus type 4:report of a swimming pool-related outbreak with recovery of virus from pool water[J].J Infect Dis,1979,140(1):42-7.
[15] Artieda J,Pineiro L,González MC,et al.A swimming pool-related outbreak of pharyngoconjunctival fever in children due to Adenovirus type 4,Gipuzkoa,Spain,2008[J].Euro Surveill,2009,14(8):442-9.
[16] Chen M,Zhu Z,Huang F,et al.Adenoviruses associated with acute respiratory diseases reported in beijing from 2011 to 2013[J].Plos One,2015,10(3):e0121375.
[17] Welch D,Nicholls GK,Rodrigo A,et al.Integrating genealogy and epidemiology:the ancestral infection and selection graph as a model for reconstructing host virus histories[J].Theor Popul Biol,2005,68(1):65-75.
[18] 杨鹏飞,燕清丽,苏琦,等.基于mtCOI基因序列的DNA条形码技术在江苏沿江长角血蜱鉴定中的应用[J].江苏预防医学,2018,29(2):135-9.
[2] 高文娟,金玉,段招军.人腺病毒的研究进展[J].病毒学报,2014,30(2):193-200.
[3] Ghebremedhin B.Human adenovirus:Viral pathogen with increasingimportance[J].Eur J Microbiol Immunol,2014,4(1):26-33.
[4] Ma Q,Tian X,Jiang Z,et al.Neutralizing epitopes mapping of human adenovirus type 14 hexon[J].Vaccine,2015,33(48):6659-65.
[5] Klein SR,Jiang H,Hossain MB,et al.Critical role of autophagy in the processing of adenovirus Capsid-Incorporated Cancer-Specific antigens[J].Plos One,2016,11(4):e0153814.
[6] Hilleman MR,Werner JH.Recovery of new agent from patients with acute respiratory illness[J].Proc Soc Exp Biol Med,1954,85(1):183-8.
[7] 黄国虹,许文波.腺病毒新型别的研究进展[J].病毒学报,2013,29(3):342-8.
[8] 田新贵,周荣.人腺病毒分型及分子进化研究进展[J].中华微生物学和免疫学杂志,2014(6):481-6.
[9] Tong HI,Lu Y.Effective detection of human adenovirus in hawaiian waters using enhanced pcr methods[J].Virol J,2011,8(1):1-6.
[10] 王媛媛,裴广倩,赵飞扬,等.腺病毒41型感染致儿童腹泻一例临床表现及病毒基因组学特征分析[J].中国病原生物学杂志,2018,13(11):1236-42.
[11] Chen M,Zhu Z,Huang F,et al.Adenoviruses associated with acute respiratory diseases reported in Beijing from 2011 to,2013[J].PLoS One,2015(10):e0121375.
[12] Echavarria M,Sanchez JL,Kolavic-Gray SA,et al.Rapid detection of adenovirus in throat swab specimens by PCR during respiratory disease outbreaks among military recruits[J].J Clin Microbiol,2003,41(2):810-2.
[13] Zhu Z,Zhang Y,Xu S,et al.Outbreak of acute respiratory disease in China caused by B2 species of adenovirus type 11[J].J Clin Microbiol,2009,47(3):697-703.
[14] D'Angelo LJ,Hierholzer JC,Keenlyside RA,et al.Pharyngoconjunctival fever caused by adenovirus type 4:report of a swimming pool-related outbreak with recovery of virus from pool water[J].J Infect Dis,1979,140(1):42-7.
[15] Artieda J,Pineiro L,González MC,et al.A swimming pool-related outbreak of pharyngoconjunctival fever in children due to Adenovirus type 4,Gipuzkoa,Spain,2008[J].Euro Surveill,2009,14(8):442-9.
[16] Chen M,Zhu Z,Huang F,et al.Adenoviruses associated with acute respiratory diseases reported in beijing from 2011 to 2013[J].Plos One,2015,10(3):e0121375.
[17] Welch D,Nicholls GK,Rodrigo A,et al.Integrating genealogy and epidemiology:the ancestral infection and selection graph as a model for reconstructing host virus histories[J].Theor Popul Biol,2005,68(1):65-75.
[18] 杨鹏飞,燕清丽,苏琦,等.基于mtCOI基因序列的DNA条形码技术在江苏沿江长角血蜱鉴定中的应用[J].江苏预防医学,2018,29(2):135-9.