花生中致敏蛋白的检测方法研究进展
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摘要
花生中含有丰富的营养成分,但也含有能引起花生过敏者发生过敏反应的致敏蛋白。花生过敏患者误食含花生食品后,将引发严重过敏反应,且没有确切有效的治疗方法。因此,建立简单、快速、灵敏检测食品中花生过敏原物质的方法尤为重要。对花生中几种主要致敏蛋白和目前常见检测方法进行综述,并对其未来发展方向进行展望,为花生致敏蛋白快速检测的深入研究提供一定的参考。
Peanuts are rich in nutrients,but also contain allergenic proteins that can cause peanut allergies. Peanut allergens will cause serious allergic reactions when they eat peanut food by mistake,and there is no effective treatment. Therefore,it is very important to establish a simple,rapid and sensitive method to detect peanut allergens in food. In this paper,several main allergenic proteins in peanut and the common detection methods were reviewed, and the future development direction was prospected,which laid a certain foundation for further study of peanut allergic protein detection.
Peanuts are rich in nutrients,but also contain allergenic proteins that can cause peanut allergies. Peanut allergens will cause serious allergic reactions when they eat peanut food by mistake,and there is no effective treatment. Therefore,it is very important to establish a simple,rapid and sensitive method to detect peanut allergens in food. In this paper,several main allergenic proteins in peanut and the common detection methods were reviewed, and the future development direction was prospected,which laid a certain foundation for further study of peanut allergic protein detection.
引文
[1]赵贵兴,陈霞,刘昊飞,等.花生的功能成分、营养价值及其开发利用研究[J].安徽农学通报,2011,34(17):39-41.
[2]Sampson H A.Food allergy.Part 1:immunopathogenesis and clinical disorders[J].J Aller Clin Immunol,1999(5):717-728.
[3]Jansen J J,Kardinaal A F,Huijbers G,et al.Prevalence of food allergy and intolerance in the adult Dutch population[J].J Aller Clin Immunol,1994(2):446-456.
[4]Mills E N C,Mackie A R,Burney P,et al.The prevalence,cost and basis of food allergy across Europe[J].Allergy,2007,62(7):717-722.
[5]Johnson P E,Sayers R L,Gethings L A,et al.Quantitative proteomic profiling of peanut allergens in food ingredients used for oral food challenges[J].Analytical Chemistry,2016(11):5 689-5 695.
[6]Croote D,Quake S R.Food allergen detection by mass spectrometry:The role of systems biology[J].Npj Syst Biol Appl,2016(2):1-10.
[7]Wensing M,Penninks A H,Hfle S L,et al.The range of minimum provoking doses in hazelnut-allergic patients as determined by double-blind placeb-controlled food challenges[J].Clinical&Experimental Allergy,2010,32(12):1 757-1 762.
[8]Cabanos C,Urabe H,Tandangsilvas M R,et al.Crystal structure of the major peanut allergen Ara h1[J].Molecular Immunology,2011,49(1-2):115-123.
[9]王宇,戚平,肖剑,等.花生蛋饮料中花生蛋白免疫检测方法的建立[J].农产品加工,2016(12):16-19.
[10]Park C W,Kim G I,Lee C H.A comparison study on allergen components between Korean(Arachis fasgigiata Shinpung)and American peanut(Arachis hypogaea Runne)r[J].Korean Med Sci,2000(15):387-392.
[11]Petersen A,Kull S,Rennert S,et al.Peanut defensins:Novel allergens isolated from lipophilic peanut extract[J].Journal of Allergy&Clinical Immunology,2015(5):1 295-1 301.
[12]van Boxtel E L,van Beers M M,Koppelman S J,et al.Allergen Ara h1 occurs in pealluts as a large oligomer rather than as a trimer[J].J Agric Food Chem,2016,54(19):7 180-7 186.
[13]Smit J J,Pennings M T,Willemsen K,et al.Heterogeneous responses and cross reactivity between the major peanut allergens Ara h 1,2,3 and 6 in a mouse model for peanut allergy[J].Clinical&Translational Allergy,2015(1):2-9.
[14]Sayers R L,Gethings L A,Lee V,et al.Microfluidic separation coupled to mass spectrometry for quantification of peanut allergens in a complex food matrix.[J].Journal of Proteome Research,2017,17(1):647-655.
[15]Boo C C,Parker C H,Jackson L S.A targeted LC-MS/MSmethod for the simultaneous detection and quantitation of egg,milk,and peanut allergens in sugar cookies[J].Journal of Aoac International,2017(1):108-117.
[16]Chen X,Wang Q,Elmezayen R,et al.Ara h2 and Ara h6 have similar allergenic activity and are substantially redundant[J].International Archives of Allergy&Immunology,2013(3):251-258.
[17]Kulis M,Chen X,Lew J,et al.The 2S albumin allergens of Arachis hypogaea,Ara h2 and Ara h6,are the major elicitors of anaphylaxis and can effectively desensitize peanut-allergic mice[J].Clinical&Experimental Allergy,2012,42(2):326-336.
[18]Maleki S J,Viquez O,Jacks T,et a1.The major peanut allergen,Ara h2,functions as a trypsin inhibitor,and roasting enhances this function[J].Allergy and Clinical Immunology,2003(1):190-195.
[19]Yusnawan E,Marquis C P,Alice L N,et al.Purification and characterization of Ara h1 and Ara h3 from four peanut market types revealed higher order oligomeric structures[J].J Agric Food Chem,2012,60(4):10 352-10 358.
[20]白卫东,沈棚,钱敏,等.花生过敏原物质及其脱敏方法研究进展[J].广东农业科学,2012(7):233-236.
[21]张雅君,张浩,杨选.花生主要致敏物质及其脱敏方法研究进展[J].食品科学,2014,35(17):312-318.
[22]Chiang W C,Pons L,KidonI M I,et al.Serological and clinical characteristics of children with peanut sensitization in an Asian community[J].Pediatr Allergy Immunol,2010(22):29-43.
[23]Anboxtel E L,Vandenbroek L A,Koppelman S J,et al.Legumin allergens from peanuts and soybeans:Effects of denaturation and aggregation on allergenicit[J].Mol Nutr Food Res,2008,52(6):74-682.
[24]Pie R R,Gaspari M,Hefle S L,et al.Proteolytic processing of the peanut allergen Ara h3[J].Mol Nutr Food Res,2005,49(8):744-755.
[25]Breiteneder H,Radauer C.A classification of plant food allergens[J].Allergy Clin Immunol.2004(5):821-830.
[26]Chen X,Wang Q,El-Mezayen R,et al.Ara h2 and Ara h6 have similar allergenic activity and are substantially redundant[J].Int Arch Allergy Immunol,2012(3):251-258.
[27]Mittag D,Akkerdaas J,Ballmer-weber B K,et al.Ara h8,a Betv1-homologous allergen from peanut is a major allergen in patients with combined birch pollen and peanut allergy[J].Journal of Allergy and Clinical Immunology,2004(6):1 410-1 417.
[28]Mittag D,Akkerdaas J,Ballmer-Weber B K,et al.Ara h 8,a Betv1 homologous allergen from peanut,is a major allergen in patients with combined birch pollen and peanut allergy[J].J Allergy Clin Immunol,2004(6):1 410-1 417.
[29]Frazier RA,Papadopoulou A,Mueller-Harvey I,et al.Probing protein-tannin interactions by isothermal titration microcalorimetry[J].J Agric Food Chem,2003,51(18):5 189-5 195.
[30]Rabjohn P,Helm EM,Stanley J S,et al.Molecular cloning and epitope analysis of the peanut allergen Ara h3[J].J Clin Invest,1999(4):535-542.
[31]荣策.花生致敏原检测技术的研究进展[J].食品安全质量检测学报,2012,3(4):255-258.
[32]闫飞,周宁菱,罗春萍,等.基于Ara h6的花生间接竞争ELISA检测方法的建立[J].食品工业科技,2012,33(17):303-306.
[33]Iqbal A,Ateeq N.Effect of processing on the detectability of peanut protein by elisa[J].Food Chem,2013(3):1 651-1 654.
[34]Wang Y,Fu T J,Howard A,et al.Crystal structure of peanut(arachis hypogaea)allergen Ara h5[J].J Agric Food Chem,2013,61(7):1 573-1 578.
[35]洪宇伟,陈启,张京顺,等.超高效液相色谱-电喷雾质谱法检测花生过敏原Ara h2[J].食品安全质量检测学报,2015(5):1 895-1 902.
[36]鲍蕾,许艳丽,梁成珠,等.表面等离子共振生物传感器在食品检测中的应用[J].检验检疫学刊,2012,22(4):73-76.
[37]Pollet J,Delport F,Janssen K P F,et al.Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor[J].Talanta,2011(5):1 436-1 441.
[38]Scaravelli E,Brohée M,Marchelli R,et al.The effect of heat treatment onthe detection of peanut allergens as determined by ELISA and real-time PCR[J].Anal Bioanal Chem,2009(1):127-137.
[39]Houhoula D,Koussisis S,Lougovois V,et al.Detection of peanut allergen traces with arreal time PCR assay-the challenge to protect food-allergic consumers[J].Journal of Food Research,2017(1):32-40.
[40]陈家杰,王海燕,梁秋妮,等.两种PCR方法检测食品中花生过敏原Ara h1成分[J].食品研究与开发,2011,32(9):69-74.
[2]Sampson H A.Food allergy.Part 1:immunopathogenesis and clinical disorders[J].J Aller Clin Immunol,1999(5):717-728.
[3]Jansen J J,Kardinaal A F,Huijbers G,et al.Prevalence of food allergy and intolerance in the adult Dutch population[J].J Aller Clin Immunol,1994(2):446-456.
[4]Mills E N C,Mackie A R,Burney P,et al.The prevalence,cost and basis of food allergy across Europe[J].Allergy,2007,62(7):717-722.
[5]Johnson P E,Sayers R L,Gethings L A,et al.Quantitative proteomic profiling of peanut allergens in food ingredients used for oral food challenges[J].Analytical Chemistry,2016(11):5 689-5 695.
[6]Croote D,Quake S R.Food allergen detection by mass spectrometry:The role of systems biology[J].Npj Syst Biol Appl,2016(2):1-10.
[7]Wensing M,Penninks A H,Hfle S L,et al.The range of minimum provoking doses in hazelnut-allergic patients as determined by double-blind placeb-controlled food challenges[J].Clinical&Experimental Allergy,2010,32(12):1 757-1 762.
[8]Cabanos C,Urabe H,Tandangsilvas M R,et al.Crystal structure of the major peanut allergen Ara h1[J].Molecular Immunology,2011,49(1-2):115-123.
[9]王宇,戚平,肖剑,等.花生蛋饮料中花生蛋白免疫检测方法的建立[J].农产品加工,2016(12):16-19.
[10]Park C W,Kim G I,Lee C H.A comparison study on allergen components between Korean(Arachis fasgigiata Shinpung)and American peanut(Arachis hypogaea Runne)r[J].Korean Med Sci,2000(15):387-392.
[11]Petersen A,Kull S,Rennert S,et al.Peanut defensins:Novel allergens isolated from lipophilic peanut extract[J].Journal of Allergy&Clinical Immunology,2015(5):1 295-1 301.
[12]van Boxtel E L,van Beers M M,Koppelman S J,et al.Allergen Ara h1 occurs in pealluts as a large oligomer rather than as a trimer[J].J Agric Food Chem,2016,54(19):7 180-7 186.
[13]Smit J J,Pennings M T,Willemsen K,et al.Heterogeneous responses and cross reactivity between the major peanut allergens Ara h 1,2,3 and 6 in a mouse model for peanut allergy[J].Clinical&Translational Allergy,2015(1):2-9.
[14]Sayers R L,Gethings L A,Lee V,et al.Microfluidic separation coupled to mass spectrometry for quantification of peanut allergens in a complex food matrix.[J].Journal of Proteome Research,2017,17(1):647-655.
[15]Boo C C,Parker C H,Jackson L S.A targeted LC-MS/MSmethod for the simultaneous detection and quantitation of egg,milk,and peanut allergens in sugar cookies[J].Journal of Aoac International,2017(1):108-117.
[16]Chen X,Wang Q,Elmezayen R,et al.Ara h2 and Ara h6 have similar allergenic activity and are substantially redundant[J].International Archives of Allergy&Immunology,2013(3):251-258.
[17]Kulis M,Chen X,Lew J,et al.The 2S albumin allergens of Arachis hypogaea,Ara h2 and Ara h6,are the major elicitors of anaphylaxis and can effectively desensitize peanut-allergic mice[J].Clinical&Experimental Allergy,2012,42(2):326-336.
[18]Maleki S J,Viquez O,Jacks T,et a1.The major peanut allergen,Ara h2,functions as a trypsin inhibitor,and roasting enhances this function[J].Allergy and Clinical Immunology,2003(1):190-195.
[19]Yusnawan E,Marquis C P,Alice L N,et al.Purification and characterization of Ara h1 and Ara h3 from four peanut market types revealed higher order oligomeric structures[J].J Agric Food Chem,2012,60(4):10 352-10 358.
[20]白卫东,沈棚,钱敏,等.花生过敏原物质及其脱敏方法研究进展[J].广东农业科学,2012(7):233-236.
[21]张雅君,张浩,杨选.花生主要致敏物质及其脱敏方法研究进展[J].食品科学,2014,35(17):312-318.
[22]Chiang W C,Pons L,KidonI M I,et al.Serological and clinical characteristics of children with peanut sensitization in an Asian community[J].Pediatr Allergy Immunol,2010(22):29-43.
[23]Anboxtel E L,Vandenbroek L A,Koppelman S J,et al.Legumin allergens from peanuts and soybeans:Effects of denaturation and aggregation on allergenicit[J].Mol Nutr Food Res,2008,52(6):74-682.
[24]Pie R R,Gaspari M,Hefle S L,et al.Proteolytic processing of the peanut allergen Ara h3[J].Mol Nutr Food Res,2005,49(8):744-755.
[25]Breiteneder H,Radauer C.A classification of plant food allergens[J].Allergy Clin Immunol.2004(5):821-830.
[26]Chen X,Wang Q,El-Mezayen R,et al.Ara h2 and Ara h6 have similar allergenic activity and are substantially redundant[J].Int Arch Allergy Immunol,2012(3):251-258.
[27]Mittag D,Akkerdaas J,Ballmer-weber B K,et al.Ara h8,a Betv1-homologous allergen from peanut is a major allergen in patients with combined birch pollen and peanut allergy[J].Journal of Allergy and Clinical Immunology,2004(6):1 410-1 417.
[28]Mittag D,Akkerdaas J,Ballmer-Weber B K,et al.Ara h 8,a Betv1 homologous allergen from peanut,is a major allergen in patients with combined birch pollen and peanut allergy[J].J Allergy Clin Immunol,2004(6):1 410-1 417.
[29]Frazier RA,Papadopoulou A,Mueller-Harvey I,et al.Probing protein-tannin interactions by isothermal titration microcalorimetry[J].J Agric Food Chem,2003,51(18):5 189-5 195.
[30]Rabjohn P,Helm EM,Stanley J S,et al.Molecular cloning and epitope analysis of the peanut allergen Ara h3[J].J Clin Invest,1999(4):535-542.
[31]荣策.花生致敏原检测技术的研究进展[J].食品安全质量检测学报,2012,3(4):255-258.
[32]闫飞,周宁菱,罗春萍,等.基于Ara h6的花生间接竞争ELISA检测方法的建立[J].食品工业科技,2012,33(17):303-306.
[33]Iqbal A,Ateeq N.Effect of processing on the detectability of peanut protein by elisa[J].Food Chem,2013(3):1 651-1 654.
[34]Wang Y,Fu T J,Howard A,et al.Crystal structure of peanut(arachis hypogaea)allergen Ara h5[J].J Agric Food Chem,2013,61(7):1 573-1 578.
[35]洪宇伟,陈启,张京顺,等.超高效液相色谱-电喷雾质谱法检测花生过敏原Ara h2[J].食品安全质量检测学报,2015(5):1 895-1 902.
[36]鲍蕾,许艳丽,梁成珠,等.表面等离子共振生物传感器在食品检测中的应用[J].检验检疫学刊,2012,22(4):73-76.
[37]Pollet J,Delport F,Janssen K P F,et al.Fast and accurate peanut allergen detection with nanobead enhanced optical fiber SPR biosensor[J].Talanta,2011(5):1 436-1 441.
[38]Scaravelli E,Brohée M,Marchelli R,et al.The effect of heat treatment onthe detection of peanut allergens as determined by ELISA and real-time PCR[J].Anal Bioanal Chem,2009(1):127-137.
[39]Houhoula D,Koussisis S,Lougovois V,et al.Detection of peanut allergen traces with arreal time PCR assay-the challenge to protect food-allergic consumers[J].Journal of Food Research,2017(1):32-40.
[40]陈家杰,王海燕,梁秋妮,等.两种PCR方法检测食品中花生过敏原Ara h1成分[J].食品研究与开发,2011,32(9):69-74.