甘草甜味素的化学成分研究
|
摘要
目的:研究提取甘草中甘草酸后剩余的副产品甘草甜味素中的化学成分,旨在探明甘草甜味素中的化学成分,拓宽其应用范围,为评价化学成分的生物活性提供科学依据。方法:采用溶剂提取、中压制备色谱和循环制备液相色谱等手段对甘草甜味素中的化学成分进行分离纯化,通过核磁共振对化合物结构进行鉴定。结果:从甘草甜味素中分离鉴定出10个化合物,分别为对羟基苯甲酸(p-hydroxybenic acid,Ⅰ)、甘草素-7,4'-二葡萄糖苷(Liquiritigenin-7,4-diglucoside,Ⅱ)、甘草素-4'-O-芹糖基-(1→2)葡萄糖苷(Liquiritigenin-apiosyl(1→2) glucoside),Ⅲ)、异甘草素葡萄糖芹菜苷(Licuraside,Ⅳ)、异甘草苷(Isoliquiritin,Ⅴ)、甘草素(Liquiritigenin,Ⅵ)、异甘草素(Isoliquiritigenin,Ⅶ)、芒柄花素(Formononetin,Ⅷ)、甘草苷(Liquiritin,Ⅸ)、刺甘草查尔酮(Echinatin,Ⅹ)。结论:该方法能分离得到10种成分,能拓宽副产品的应用范围。
Objective: The chemical constituents of licorice sweeteners which were the by-products after glycyrrhizic acid extracted were stuided to ascertain the chemical constituents of licorice sweeteners,widen its application scope and provide a scientific basis for evaluating the biological activity of the chemical components.Methods: The chemical constituents of licorice sweeteners were separated and purified by solvent extraction,medium pressure preparative chromatography and recycling preparation liquid chromatography. Meanwhile,NMR methods were employed to identify their structures.Results: Ten compounds were isolated and identified as p-hydroxybenic acid,Liquiritigenin-7,4'-diglucoside, mLiquiritigenin-apiosyl( 1 → 2) glucoside,Licuraside,Isoliquiritin,Liquiritigenin,Isoliquiritigenin,Formononetin,Liquiritin,Echinatin.Conclusion,The method can separate 10 components and widen the application range of by-products.
Objective: The chemical constituents of licorice sweeteners which were the by-products after glycyrrhizic acid extracted were stuided to ascertain the chemical constituents of licorice sweeteners,widen its application scope and provide a scientific basis for evaluating the biological activity of the chemical components.Methods: The chemical constituents of licorice sweeteners were separated and purified by solvent extraction,medium pressure preparative chromatography and recycling preparation liquid chromatography. Meanwhile,NMR methods were employed to identify their structures.Results: Ten compounds were isolated and identified as p-hydroxybenic acid,Liquiritigenin-7,4'-diglucoside, mLiquiritigenin-apiosyl( 1 → 2) glucoside,Licuraside,Isoliquiritin,Liquiritigenin,Isoliquiritigenin,Formononetin,Liquiritin,Echinatin.Conclusion,The method can separate 10 components and widen the application range of by-products.
引文
[1]张明发,沈雅琴.甘草酸及其苷元甘草次酸的糖皮质激素样作用[J].现代药物与临床,2011,26(1):33-35.
[2]苏俊喜,周颖,闫文军,等.甘草有效成分应用的研究进展[J].畜牧与饲料科学,2010,31(8):111-113.
[3]王锡茂,黎彧,何妙银,等.甜味剂开发的研究进展[J].广东微量元素科学,2012,19(5):27-31.
[4]王秀锋,单风平.甘草甜素抗炎及抗肿瘤机制研究进展[J].微生物学杂志,2013,33(4):88-92.
[5]Sidana J,Joshi l K.Recycle HPLC:A powerful tool for the purification of natural products[J].Chromatography Research International,2013,11(6):1-7.
[6]关颖丽,尹虹,刘建宇,等.循环制备分离纯化朝鲜白头翁中三萜皂苷[J].中草药,2008,39(11):1628-1631.
[7]李国柱,孟庆艳,罗碧,等.循环制备液相色谱分离芳香新塔花中的化学成分[J].色谱,2015,33(1):84-89.
[8]白虹.栽培乌拉尔甘草和直立白薇的化学成分研究[D].沈阳:沈阳药科大学,2005.
[9]Yahara S,Nishioka I.Flavonoid glucosides from licorice[J].Phytochemistry,1984,23(9):2108-2109.
[10]Liu Q,Liu Y.Application of 13CNMR to structural identification of the flavonoid glycosides[J].Zhongguo Yi Xue Ke Xue Yuan Xue Bao,1990,12(5):359-364.
[11]王英华,白虹,窦德强,等.栽培甘草中黄酮类成分的研究[J].西北药学杂志,2004,19(6):252-253.
[12]孙明谦.甘草中化学成分的研究[D].吉林:吉林大学,2006.
[13]畅行若,徐清河,朱大元,等.甘草新木脂素的分离与化学结构[J].药学学报,1983,18(1):45-50.
[14]Fu B,Li H,Wang X,et al.Isolation and identification of flavonoids in licorice and a study of their inhibitory effects on tyrosinase[J].Journal of Agricultural&Food Chemistry,2005,53(19):7408-7414.
[2]苏俊喜,周颖,闫文军,等.甘草有效成分应用的研究进展[J].畜牧与饲料科学,2010,31(8):111-113.
[3]王锡茂,黎彧,何妙银,等.甜味剂开发的研究进展[J].广东微量元素科学,2012,19(5):27-31.
[4]王秀锋,单风平.甘草甜素抗炎及抗肿瘤机制研究进展[J].微生物学杂志,2013,33(4):88-92.
[5]Sidana J,Joshi l K.Recycle HPLC:A powerful tool for the purification of natural products[J].Chromatography Research International,2013,11(6):1-7.
[6]关颖丽,尹虹,刘建宇,等.循环制备分离纯化朝鲜白头翁中三萜皂苷[J].中草药,2008,39(11):1628-1631.
[7]李国柱,孟庆艳,罗碧,等.循环制备液相色谱分离芳香新塔花中的化学成分[J].色谱,2015,33(1):84-89.
[8]白虹.栽培乌拉尔甘草和直立白薇的化学成分研究[D].沈阳:沈阳药科大学,2005.
[9]Yahara S,Nishioka I.Flavonoid glucosides from licorice[J].Phytochemistry,1984,23(9):2108-2109.
[10]Liu Q,Liu Y.Application of 13CNMR to structural identification of the flavonoid glycosides[J].Zhongguo Yi Xue Ke Xue Yuan Xue Bao,1990,12(5):359-364.
[11]王英华,白虹,窦德强,等.栽培甘草中黄酮类成分的研究[J].西北药学杂志,2004,19(6):252-253.
[12]孙明谦.甘草中化学成分的研究[D].吉林:吉林大学,2006.
[13]畅行若,徐清河,朱大元,等.甘草新木脂素的分离与化学结构[J].药学学报,1983,18(1):45-50.
[14]Fu B,Li H,Wang X,et al.Isolation and identification of flavonoids in licorice and a study of their inhibitory effects on tyrosinase[J].Journal of Agricultural&Food Chemistry,2005,53(19):7408-7414.