农产品中真菌毒素样品前处理和检测方法研究进展
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摘要
真菌毒素是由真菌产生广泛存在于农产品中的有毒次生代谢产物。真菌毒素污染日益加重,世界各国真菌毒素限量要求逐年降低,迫切需要更加灵敏、更加准确的真菌毒素确证性检测技术。系统综述了农产品中真菌毒素样品前处理方法和检测技术,前处理技术包括:液-液萃取技术、固相萃取技术、QuEChERS前处理技术等;检测技术包括:高效液相色谱法、液相色谱-质谱联用法、液相色谱-同位素稀释质谱法等。并对真菌毒素样品前处理和检测方法进行了总结和展望。
Mycotoxins are toxic metabolites that produced by the fungus and are widely existing in agricultural products.Mycotoxin pollution is becoming increasingly serious,and the limit of Mycotoxin in various countries is decreasing year by year.There is an urgent need for more sensitive and accurate assay techniques for Mycotoxin.The methods for sample preparation and detection of Mycotoxins in agricultural products were systematically reviewed.The preparation techniques include: liquid-liquid extraction,solid phase extraction,Qu ECh ERS preparation technique,and so on; Detection techniques include: high performance liquid chromatography,liquid chromatography-isotope dilution mass spectrometry,liquid chromatography-mass spectrometry and the like. Finally,the sample preparation and detection methods for Mycotoxin were summarized and prospected.
Mycotoxins are toxic metabolites that produced by the fungus and are widely existing in agricultural products.Mycotoxin pollution is becoming increasingly serious,and the limit of Mycotoxin in various countries is decreasing year by year.There is an urgent need for more sensitive and accurate assay techniques for Mycotoxin.The methods for sample preparation and detection of Mycotoxins in agricultural products were systematically reviewed.The preparation techniques include: liquid-liquid extraction,solid phase extraction,Qu ECh ERS preparation technique,and so on; Detection techniques include: high performance liquid chromatography,liquid chromatography-isotope dilution mass spectrometry,liquid chromatography-mass spectrometry and the like. Finally,the sample preparation and detection methods for Mycotoxin were summarized and prospected.
引文
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[3]石晓敏,丁亮.粮油中真菌毒素检测技术及其应用[J].现代食品,2018,(16):108-109.
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[8]李建忠,王海波.生物毒素研究进展[J].食品安全质量检测学报,2018,9(13):3 359-3 367.
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[28]陈建彪,董丽娜,刘娇,等.Qu ECh ERS在食品中真菌毒素检测的研究进展[J].食品科学,2014,35(11):286-291.
[29]薛焕,金雁,于永丽.超高效液相色谱-串联三重四极杆线性离子阱质谱法同时检测猪肉中33种兽药残留[J].分析科学学报,2018,34(3):387-392.
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[37]BASCARAN V,DE ROJAS A H,CHOUCINO P,et al.Analysis of ochratoxin A in milk after direct immunoaffinity column clean-up by high-performance liquid chromatography with fluorescence detection[J]. J. Chromatogr.A,2007,1 167(1):95-101.
[38]SHEPHARD G S.Chromatographic determination of the fumonisin Mycotoxins[J]. J. Chromatogr. A,1998,815(1):31-39.
[39]AMBROSINO P,GALVANO F,FOGLIANO V,et al.Supercritical fluid extraction of beauvericin from maize[J].Talanta,2004,62(3):523-530.
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[42]沈思捷,殷文.液质联用技术在水质检测中的应用研究进展[J].中国资源综合利用,2018,36(12):109-111.
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[45]YIBADATIHAN S,JINAP S,MAHYUDIN N A.Simultaneous determination of multi-Mycotoxins in palm kernel cake(PKC)using liquid chromatography-tandem mass spectrometry(LC-MS/MS)[J]. Food Addit. Contam. A,2014,31(12):2 071-2 079.
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[2]李蓉,黄莹偲,王勇,等.食品中真菌毒素检测技术的研究进展[J].中国卫生检验杂志,2015,25(18):3 195-3 198.
[3]石晓敏,丁亮.粮油中真菌毒素检测技术及其应用[J].现代食品,2018,(16):108-109.
[4]TURNER N W,BRAMHMBHATT H,SZABO-VEZSE M,et al.Analytical methods for determination of Mycotoxins:an update(2009-2014)[J]. Anal. Chim. Acta,2015,901(2):12-33.
[5]BHAT R,RAI R V,KARIM A A.Mycotoxins in food and feed:present status and future concerns[J]. Compr. Rev.Food Sci.F,2010,9(1):57-81.
[6]国家食品药品监督管理总局.食品安全国家标准食品中真菌毒素限量:GB 2761—2017[S].2017.
[7]宋卫得,苏征,惠希东,等.液质联用技术在食品真菌毒素检测中的研究进展[J].食品工业科技,2016,37(17):395-399.
[8]李建忠,王海波.生物毒素研究进展[J].食品安全质量检测学报,2018,9(13):3 359-3 367.
[9]KNOLHOFF A M,CROLEY T R.Non-targeted screening approaches for contaminants and adulterants in food using liquid chromatography hyphenated to high resolution mass spectrometry[J].J.Chromatogr.A,2016,1 428:86-96.
[10]YANG J,LI J,JIANG Y,et al.Natural occurrence,analysis,and prevention of Mycotoxins in fruits and their processed products[J]. Crit. Rev. Food Sci.,2014,54(1):64-83.
[11]傅勇,曹纪亮,杨小丽,等.真菌毒素检测的前处理技术及其应用[J].中国卫生检验杂志,2012,22(6):1 462-1 466.
[12]杨琳,张宇昊,马良.高效液相色谱法同时检测粮谷中的黄曲霉毒素和赭曲霉毒素[J].食品科学,2010,31(24):250-254.
[13]SLOBODCHIKOVA I,VUCKOVIC D.Liquid chromatography-high resolution mass spectrometry method for monitoring of 17 Mycotoxins in human plasma for exposure studies[J].J.Chromatogr.A,2018,1 548:51-63.
[14]HUERTAS-PEREZ J F,ARROYO-MANZANARES N,GARCIA-CAMPANA A M,et al. Solid phase extraction as sample treatment for the determination of ochratoxin A in foods:a review[J].Crit.Rev.Food Sci. Nutr.,2016,57(16):3 405-3 420.
[15]VATINNO R,VUCKOVIC D,ZAMBONIN C G,et al.Automated high-throughput method using solid-phase microextraction-liquid chromatography-tandem mass spectrometry for the determination of ochratoxin A in human urine[J]. J. Chromatogr. A,2008,1201(2):215-221.
[16]KATERERE D R,STOCKENSTROM S,SHEPHARD G S.HPLC-DAD method for the determination of patulin in dried apple rings[J]. Food Control,2008,19(4):389-392.
[17]GIRAUDI G,ANFOSSI L,BAGGIANI C,et al. Solidphase extraction of ochratoxin A from wine based on a binding hexapeptide prepared by combinatorial synthesis[J].J.Chromatogr.A,2007,1 175(2):174-180.
[18]SHARMA M,MARQUEZ C.Determination of Aflatoxins in domestic pet foods(dog and cat)using immunoaffinity column and HPLC[J]. Anim. Feed Sci. Tech.,2001,93(1):109-114.
[19]VISCONTI A,PASCALE M,CENTONZE G.Determination of ochratoxin A in domestic and imported beers in Italy by immunoaffinity clean-up and liquid chromatography[J].J.Chromatogr.A,2000,888(1):321-326.
[20]VISCONTI A, PASCALE M. Determination of zearalenone in corn by means of immunoaffinity clean-up and high-performance liquid chromatography with fluorescence detection[J]. J. Chromatogr. A,1998,815(1):133-140.
[21]王恒玲,喻理,李培武,等.二氧化硅-氧化石墨烯复合物固相萃取-高效液相色谱法检测植物油中黄曲霉毒素B-1、B-2[J].分析化学,2014,42(9):1 338-1 342.
[22]COSTA R. Newly introduced sample preparation techniques:towards miniaturization[J]. Crit. Rev. Anal.Chem.,2014,44(4):299-310.
[23]HU Y Y,ZHENG P,ZHANG Z X,et al.Determination of Aflatoxins in high-pigment content samples by matrix solid-phase dispersion and high-performance liquid chromatography[J]. J. Agr. Food Chem.,2006,54(12):4 126-4 130.
[24]龙星宇,吴迪,龚小见,等.功能化有机聚合物磁球对金属离子磁固相萃取的研究进展[J].化工新型材料,2018,46(10):49-52.
[25]ANASTASSIADES M,LEHOTAY S J,STAJNBAHER D,et al. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and“dispersive solidphase extraction”for the determination of pesticide residues in produce[J]. J. Aoac. Int.,2003,86(2):412-431.
[26]鹿剑,任水英.Qu ECh ERS方法在食品中多组分农残检测中的研究进展[J].中国酿造,2016,35(9):28-32.
[27]袁雪婵.QuEChERS方法及其在食品农药多残留分析中的应用[J].中国食品添加剂,2009,(2):144-148.
[28]陈建彪,董丽娜,刘娇,等.Qu ECh ERS在食品中真菌毒素检测的研究进展[J].食品科学,2014,35(11):286-291.
[29]薛焕,金雁,于永丽.超高效液相色谱-串联三重四极杆线性离子阱质谱法同时检测猪肉中33种兽药残留[J].分析科学学报,2018,34(3):387-392.
[30]梁飞燕,张科明,卢日刚. Qu ECh ERS方法净化UHPLC-MS检测猪肉中35种兽药残留[J].安徽农业科学,2016,44(25):41-45.
[31]DESMARCHELIER A,OBERSON J M,TELLA P,et al.Development and comparison of two multiresidue methods for the analysis of 17 Mycotoxins in cereals by liquid chromatography electrospray ionization tandem mass spectrometry[J]. J. Agric. Food Chem.,2010,58(13):7 510-7 519.
[32]史娜,侯彩云,路勇,等.QuEChERS-高效液相色谱-质谱法检测食品中14种真菌毒素[J].食品科学,2014,35(16):190-196.
[33]HUANG X J,WANG S M,MAO D,et al. Optimized Qu ECh ERS method combined with UHPLC-MS/MS for the simultaneous determination of 15 Mycotoxins in liquorice[J].J.Aoac.Int.,2018,101(3):633-642.
[34]ROMERO-GONZALEZ R,FRENICH A G,VIDAL J L M,et al. Simultaneous determination of pesticides,biopesticides and Mycotoxins in organic products applying a quick,easy,cheap,effective,rugged and safe extraction procedure and ultra-high performance liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2011,1 218(11):1 477-1 485.
[35]ZHANG J M,WU Y L,LU Y B.Simultaneous determination of carbamate insecticides and Mycotoxins in cereals by reversed phase liquid chromatography tandem mass spectrometry using a quick,easy,cheap,effective,rugged and safe extraction procedure[J]. J. Chromatogr. B,2013,(915/916):13-20.
[36]RUBERT J,FAPOHUNDA S O,SOLER C,et al.A survey of Mycotoxins in random street-vended snacks from Lagos,Nigeria,using Qu ECh ERS-HPLC-MS/MS[J].Food Control,2013,32(2):673-677.
[37]BASCARAN V,DE ROJAS A H,CHOUCINO P,et al.Analysis of ochratoxin A in milk after direct immunoaffinity column clean-up by high-performance liquid chromatography with fluorescence detection[J]. J. Chromatogr.A,2007,1 167(1):95-101.
[38]SHEPHARD G S.Chromatographic determination of the fumonisin Mycotoxins[J]. J. Chromatogr. A,1998,815(1):31-39.
[39]AMBROSINO P,GALVANO F,FOGLIANO V,et al.Supercritical fluid extraction of beauvericin from maize[J].Talanta,2004,62(3):523-530.
[40]WANG Y,CHAI T,LU G,et al.Simultaneous detection of airborne Aflatoxin,ochratoxin and zearalenone in a poultry house by immunoaffinity clean-up and high-performance liquid chromatography[J].Environ.Res.,2008,107(2):139-144.
[41]KARBANCIOGLU GULER F,HEPERKAN D. Natural occurrence of ochratoxin A in dried figs[J].Anal.Chim.Acta,2008,617(1):32-36.
[42]沈思捷,殷文.液质联用技术在水质检测中的应用研究进展[J].中国资源综合利用,2018,36(12):109-111.
[43]NIELSEN K F,SMEDSGAARD J. Fungal metabolite screening:database of 474 Mycotoxins and fungal metabolites for dereplication by standardised liquid chromatography-UV-mass spectrometry methodology[J]. J. Chromatogr.A,2003,1 002(1):111-136.
[44]RASMUSSEN R R,STORM I M,RASMUSSEN P H,et al.Multi-Mycotoxin analysis of maize silage by LC-MS/MS[J].Anal.Bioanal.Chem.,2010,397(2):765-776.
[45]YIBADATIHAN S,JINAP S,MAHYUDIN N A.Simultaneous determination of multi-Mycotoxins in palm kernel cake(PKC)using liquid chromatography-tandem mass spectrometry(LC-MS/MS)[J]. Food Addit. Contam. A,2014,31(12):2 071-2 079.
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