香菇多糖提取动力学模型研究
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摘要
以Fick第二扩散定律中的球形模型为基础,推导香菇多糖热水浸提动力学模型,采用苯酚-硫酸法测定香菇的不同质量浓度、提取温度、提取时间下多糖提取液的浓度,拟合动力学方程并求解相关动力学参数.香菇多糖的浸出量随着温度的升高而增加、随着香菇质量浓度的变化而变化、随着提取时间的增加而增加并达到平衡.研究得到的动力学模型可为优化香菇多糖的提取工艺条件奠定理论基础.
To establish a kinetic model for water decoction process of Lentinan,according to Fick's second law,the kinetic model was investigated based on the ball type model mechanism of extraction.The phenol-sulfuric acid colorimetry is used to determine the different of Lentinan under different temperature,meanwhile,the effective related kinetic parameters were determined.The result shows that the concentration of Lentinanincreases with the rise of temperature and also is influenced by both the ratios of solvent to solid and extraction time.The three factors of which have the same effect on the diffusion coefficient.The kinetic model established lays the foundation for the optimization of technological conditions.
To establish a kinetic model for water decoction process of Lentinan,according to Fick's second law,the kinetic model was investigated based on the ball type model mechanism of extraction.The phenol-sulfuric acid colorimetry is used to determine the different of Lentinan under different temperature,meanwhile,the effective related kinetic parameters were determined.The result shows that the concentration of Lentinanincreases with the rise of temperature and also is influenced by both the ratios of solvent to solid and extraction time.The three factors of which have the same effect on the diffusion coefficient.The kinetic model established lays the foundation for the optimization of technological conditions.
引文
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[18]李兰兰,张宇,赵统超,等.超声波辅助提取白鲜皮多糖的动力学模型研究[J].辽宁中医杂志,2018,45(6):1247-1250.doi:10.13192/j.issn.1000-1719.2018.06.041LI Lanlan,ZHANG Yu,ZHAO Tongchao,et al.Kinetics Study on Ultrasonic-assisted Extraction of Polysaccharide from Dictamnus dasycarpus Turcz[J].Liaoning Journal of Traditional Chinese Medicine,2018,45(6):1247-1250.
[19]马海乐,李前进,李树君,等.脱脂米糠中脉冲超声辅助提取蛋白和多糖动力学模型[J].农业机械学报,2011,42(11):135-138.doi:10.3969/j.issn.1000-1298.2011.11.025MA Haile,LI Qianjin,LI Shujun,et al.Kinetics Study on Pulsed Ultrasound-assisted Extraction of Protein and Polysaccharide from Defatted Rice Bran[J].Transactions of the Chinese Society of Agricultural Machinery,2011,42(11):135-138.
[20]张金生,杨宏娟,李丽华,等.超声波辅助提取益母草中多糖过程的动力学模型[J].食品工业科技,2012,33(9):131-136.doi:10.13386/j.issn.1002-0306.2012.09.030ZHANG Jinsheng,YANG Hongjuan,LI Lihua,et al.Kinetic Model of Ultrasonic Extraction Process of Polysaccharide in Leonurus Japonicus Houtt[J].Science and Technology of Food Industry,2012,33(9):131-136.
[21]陆超群.韭菜护肾活性物质的筛选及提取动力学模型研究[D].合肥:合肥工业大学,2015.LU Chaoqun.The Screening and Kinetic Model for Ultrasound-assisted Extraction of Allium Tuberosum Components A-gainst Kidney Disease[D].Hefei:Hefei University of Technology,2005.
[22]刘敏.玉竹水溶性多糖的提取动力学模型及一级结构鉴定研究[D].广州:广东药学院,2015.LIU Min.Kinetic Model of Extraction and Structural Characterization of Water-soluble Polysaccharide from Polygonatum Odoratum[D].Guangzhou:Guangdong Pharmaceutical University,2015.
[23]刘存菊,李稳宏,雒羽,等.飞天蜈蚣七多糖提取动力学研究[J].化学工程,2012,40(7):1-5.doi:10.3969/j.issn.1005-9954.2012.07.001LIU Cunju,LI Wenhong,LUO Yu,et al.Kinetic Model for Extraction of Polysaccharide from Aralia Chinensis[J].Chemical Engineering,2012,40(7):1-5.
[24]刘敏,李钟,李雪银,等.水煎煮法提取玉竹多糖的动力学模型研究[J].广东药学院学报,2015,31(2):161-166.doi:10.3969/j.issn.1006-8783.2015.02.005LIU Min,LI Zhong,LI Xueyin,et al.Study on Kinetic Model of Extracting Polysaccharide from Polygonatum Odoratum[J].Journal of Guangdong Pharmaceutical University,2015,31(2):161-166.
[25]杨勇杰,姜瑞芝,陈英红,等.苯酚-硫酸法测定杂多糖含量的研究[J].中成药,2005,27(6):706-708.doi:10.3969/j.issn.1001-1528.2005.06.027YANG Yongjie,JIANG Ruizhi,CHEN Yinghong,et al.Determination of Sugars in Heteropolysaccharide by Phenol-sulfuric Acid Method[J].Chinese Traditional Patent Medicine,2005,27(6):706-708.
[26]张朝晖,吴叶明,江蕾.红豆杉多糖浸提过程动力学模型[J].浙江大学学报(理学版),2008,35(2):223-228.doi:10.3785/j.issn.1008-9497.2008.02.022ZHANG Chaohui,WU Yeming,JIANG Lei.Kinetic Model for Polysaccharide's Extraction from Taxus by Water[J].Journal of Zhejiang University(Science Edition),2008,35(2):223-228.
[2]WANG Jie,WANG Hongyan,XIA Xiaoming,et al.Inhibitory Effect of Sulfated Lentinan and Lentinan Against Tobacco Mosaic Virus(TMV)in Tobacco Seedlings[J].Biological Macromolecules,2013,61(10):264-269.doi:10.1016/j.ijbiomac.2013.07.005
[3]LU M K,CHENG J J,LIN C Y,et al.Purification,Structural Elucidation and Anti-inflammatory Effect of a Water-soluble1,6-branchcd 1,3-α-D-galactan from Cultured Mycelia of Poria Cocos,Food[J].Chem,2010,118(2):349-356.doi:10.1016/j.foodchem.2009.04.126.
[4]HUANG X Q,TU Z C,JIANG Y,et al.Dynamic High Pressure Microfluidization-assisted Extraction and Antioxidant Activities of Lentinan[J].International Journal of Biological Macromolecules,2012,51(7):926-932.doi:10.1016/j.ijbiomac.2012.07.018
[5]LIU Y H,MA S D,FU Q J,et al.Effect of Lentinan on Membrane-bound Protein Expression in Splenic Lymphocytes Under Chronic Low-dose Radiation[J].International Immunopharmacology,2014,22(7):505-514.doi:10.1016/j.intimp.2014.07.027
[6]YOSHIDA O,NAKASHIMA H,YOSHIDA T,et al.Sulfation of the Immunomodulating Polysaccharide Lentinan:A Novel Strategy for Antivirals to Human Immunodeficiency Virus(HIV)[J].Biochem Pharmacol,1988,37(15):2887-2891.doi:10.1016/0006-2952(88)90272-9
[7]XU X,YASUDA M,NAKAMURA-TSURUTA S,et al.β-glucan from Lentinus Edodes Inhibits Nitric Oxide and Tumor Necrosis Factor:A Production and Phosphorylation of Mitogen-activated Protein Kinases in Lipopolysaccharide-stimulated Murine RAW 264.7Macrophages[J].J Biol Chem,2012,287(2):871-878.doi:10.1074/jbc.M111.297887
[8]XU X,PAN C,ZHANG L,et al.Immunomodulatory β-glucan from Lentinus Edodes Activates Mitogen-activated Protein Kinases and Nuclear Factor-κB in Murine RAW 264.7 Macrophages[J].J Biol Chem,2011,286(36):31194-31198.doi:10.1074/jbc.M111.246470
[9]YAP A,NG M.An Improved Method for the Isolation of Lentinan from the Edible and Medicinal Shiitake Mushroom,Lentinus Edodes(Berk,)Sing.(Agaricomycctideae)[J].Int J Med Mushrooms,2001,3(1):6-19.doi:10.1615/IntJMed Mushr.v3.i1.20
[10]梁敏,邬洪源.复合酶法提取香菇多糖[J].高师理科学刊,2008,28(6):72-74.doi:10.3969/j.issn.1007-9831.2008.06.023LIANG Min,WU Hongyuan.Lentinan Extraction by Composite Enzyme[J].Journal of Science of Teachers'College and University,2008,28(6):72-74.
[11]ZHANG Y,LI S,WANG X,et al.Advances in Lentinan:Lsolation,Structure,Chain Conformation and Bioactivities[J].Food Hydrocolloid,2011,25(2):196-206.doi:10.1016/j.foodhyd.2011.02.001
[12]ZHANG L,LI X,ZHOU Q,et al.Transition from Triple Helix to Coil of Lentinan in Solution Measured by SEC,Viscometry,and 13 CNMR[J].Polym J,2002,34(6):443-449.doi:10.1295/polymj.34.443
[13]XU X,ZHANG X,ZHANG L,et al.Collapse and Association of Denatured Lentinan in Water/dimethlysulfoxide Solutions[J].Biomacromolecules,2004,5(5):1893-1898.doi:10.1021/bm049785h
[14]WANG X,ZHANG Y,ZHANG L,et al.Multiple Conformation Transitions of Triple Helical Lentinan in DMSO/Water by Microcalorimetry[J].J Phys Chem B,2009,113(29):9915-9923.doi:10.1021/jp811289y
[15]ZHANG X,ZHANG L,XU X.Morphologies and Conformation Transition of Lentinan in Aqueous NaOH Solution[J].Biopolymers,2004,75(2):187-195.doi:10.1002/bip.20112
[16]WANG X,XU X,ZHANG L.Thermally Induced Conformation Transition of Triplc-helical Lentinan in NaCl Aqueous Solution[J].J Phys Chem B,2008,112(33):10343-10351.doi:10.1021/jp802174v
[17]程伟,秦俊哲,杜军国.桑黄多糖提取过程模型的建立与动力学分析[J].现代食品科技,2013,29(3):513-518.doi:10.13982/j.mfst.1673-9078.2013.03.027CHENG Wei,QIN Junzhe,DU Junguo.Model Establishment and Kinetic Analysis of Polysaccharide from the Fruits of Phellinus Igniarius[J].Modern Food Science and Technology,2013,29(3):513-518.
[18]李兰兰,张宇,赵统超,等.超声波辅助提取白鲜皮多糖的动力学模型研究[J].辽宁中医杂志,2018,45(6):1247-1250.doi:10.13192/j.issn.1000-1719.2018.06.041LI Lanlan,ZHANG Yu,ZHAO Tongchao,et al.Kinetics Study on Ultrasonic-assisted Extraction of Polysaccharide from Dictamnus dasycarpus Turcz[J].Liaoning Journal of Traditional Chinese Medicine,2018,45(6):1247-1250.
[19]马海乐,李前进,李树君,等.脱脂米糠中脉冲超声辅助提取蛋白和多糖动力学模型[J].农业机械学报,2011,42(11):135-138.doi:10.3969/j.issn.1000-1298.2011.11.025MA Haile,LI Qianjin,LI Shujun,et al.Kinetics Study on Pulsed Ultrasound-assisted Extraction of Protein and Polysaccharide from Defatted Rice Bran[J].Transactions of the Chinese Society of Agricultural Machinery,2011,42(11):135-138.
[20]张金生,杨宏娟,李丽华,等.超声波辅助提取益母草中多糖过程的动力学模型[J].食品工业科技,2012,33(9):131-136.doi:10.13386/j.issn.1002-0306.2012.09.030ZHANG Jinsheng,YANG Hongjuan,LI Lihua,et al.Kinetic Model of Ultrasonic Extraction Process of Polysaccharide in Leonurus Japonicus Houtt[J].Science and Technology of Food Industry,2012,33(9):131-136.
[21]陆超群.韭菜护肾活性物质的筛选及提取动力学模型研究[D].合肥:合肥工业大学,2015.LU Chaoqun.The Screening and Kinetic Model for Ultrasound-assisted Extraction of Allium Tuberosum Components A-gainst Kidney Disease[D].Hefei:Hefei University of Technology,2005.
[22]刘敏.玉竹水溶性多糖的提取动力学模型及一级结构鉴定研究[D].广州:广东药学院,2015.LIU Min.Kinetic Model of Extraction and Structural Characterization of Water-soluble Polysaccharide from Polygonatum Odoratum[D].Guangzhou:Guangdong Pharmaceutical University,2015.
[23]刘存菊,李稳宏,雒羽,等.飞天蜈蚣七多糖提取动力学研究[J].化学工程,2012,40(7):1-5.doi:10.3969/j.issn.1005-9954.2012.07.001LIU Cunju,LI Wenhong,LUO Yu,et al.Kinetic Model for Extraction of Polysaccharide from Aralia Chinensis[J].Chemical Engineering,2012,40(7):1-5.
[24]刘敏,李钟,李雪银,等.水煎煮法提取玉竹多糖的动力学模型研究[J].广东药学院学报,2015,31(2):161-166.doi:10.3969/j.issn.1006-8783.2015.02.005LIU Min,LI Zhong,LI Xueyin,et al.Study on Kinetic Model of Extracting Polysaccharide from Polygonatum Odoratum[J].Journal of Guangdong Pharmaceutical University,2015,31(2):161-166.
[25]杨勇杰,姜瑞芝,陈英红,等.苯酚-硫酸法测定杂多糖含量的研究[J].中成药,2005,27(6):706-708.doi:10.3969/j.issn.1001-1528.2005.06.027YANG Yongjie,JIANG Ruizhi,CHEN Yinghong,et al.Determination of Sugars in Heteropolysaccharide by Phenol-sulfuric Acid Method[J].Chinese Traditional Patent Medicine,2005,27(6):706-708.
[26]张朝晖,吴叶明,江蕾.红豆杉多糖浸提过程动力学模型[J].浙江大学学报(理学版),2008,35(2):223-228.doi:10.3785/j.issn.1008-9497.2008.02.022ZHANG Chaohui,WU Yeming,JIANG Lei.Kinetic Model for Polysaccharide's Extraction from Taxus by Water[J].Journal of Zhejiang University(Science Edition),2008,35(2):223-228.