辣木籽中酚酸化合物的提取工艺
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摘要
为优化辣木籽中酚酸化合物的提取工艺条件,以甲醇为提取溶剂,辣木籽中没食子酸和鞣花酸的提取量为响应值,在单因素基础上,利用中心组合设计(CCD)响应面法,研究各因素及其交互作用对酚酸化合物提取量的影响,并优化提取工艺。研究表明,辣木籽中没食子酸和鞣花酸的最佳提取工艺条件为:提取时间76 min、提取温度65℃、液料比1︰33 (g/mL),在此条件下进行提取,没食子酸的最大提取量为0.104 9 mg/g、鞣花酸的提取量为0.131 7mg/g,与响应面(RSM)模型预测值接近,为辣木籽中没食子酸和鞣花酸的提取提供工艺指导。
In order to optimize the extraction process condition of the gallic acid and ellagic acid in the Moringaoleifrea seed,the influence of different factor and their interactions were studied by using single factor experiment and central composite response surface method with methanol as extraction solvent and the extraction of gallic acid and ellagic acid quantity as the response value. The results showed that the optimum process condition of extracting phenolic compounds in the Moringaoleifrea seed was as follows: extracting time 76 min, extraction temperature 65 ℃ and the ratio of material to solvent 1︰33(g/mL).In this condition, the extraction amount of gallic acid and ellagic acid was 0.104 9 mg/g and 0.131 7 mg/g, respectively, which was not significant difference compared to the response surface method(RSM) model predicted. The technical guidance for the extraction of gallic acid and ellagic acid in the Moringaoleifrea seed was provided.
In order to optimize the extraction process condition of the gallic acid and ellagic acid in the Moringaoleifrea seed,the influence of different factor and their interactions were studied by using single factor experiment and central composite response surface method with methanol as extraction solvent and the extraction of gallic acid and ellagic acid quantity as the response value. The results showed that the optimum process condition of extracting phenolic compounds in the Moringaoleifrea seed was as follows: extracting time 76 min, extraction temperature 65 ℃ and the ratio of material to solvent 1︰33(g/mL).In this condition, the extraction amount of gallic acid and ellagic acid was 0.104 9 mg/g and 0.131 7 mg/g, respectively, which was not significant difference compared to the response surface method(RSM) model predicted. The technical guidance for the extraction of gallic acid and ellagic acid in the Moringaoleifrea seed was provided.
引文
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[2]盘李军,刘小金.辣木的栽培及开发利用研究进展[J].广东林业科技,2010,26(3):71-77.
[3]LEONE A,SPADA A,BATTEZZATI A,et al.Cultivation,genetic,ethnopharmacology,phytochemistry and pharmacology of Moringa oleifera leaves:An overview[J].Internationa Journal of Molecular Sciences,2015,16(6):12791-12835.
[4]NADEEM M,IMRAN M.Promising features of Moringa oleifera oil:Recent updates and perspectives[J].Lipids in Health and Disease,2016,15(1):212.
[5]许敏,赵三军,宋晖,等.辣木的研究进展[J].食品科学,2016,37(23):291-301.
[6]ASGHARI G,PALIZBAN A,BAKHSHAEI B.Quantitative analysis of the nutritional components in leaves and seeds of the Persian Moringa peregrina(Forssk)Fiori[J].Pharmacognosy Research,2015,7(3):242-248.
[7]SAINI R K,SIVANESAN I,KEUM Y S.Phytochemicals of Moringa oleifera:A review of their nutritional,therapeutic and industrial significance[J].3 Biotech,2016,6:1-14.
[8]苏科巧,陶亮,黄艾祥.辣木食品研究进展[J].农产品加工,2015(1):72-74.
[9]OYEYINKAA A T,OYEYINKAB S A.Moringa oleifera as a food fortificant:Recent trends and prospects[J].Journal of the Saudi Society of Agricultural Sciences,2016,17(2):127-136.
[10]DEZFOOLI S M,UVERSKY V N,SALEEM M,et al.Asimplified method for the purification of an intrinsically disordered coagulant protein from defatted Moringa oleifera seeds[J].Process Biochemistry,2016,51(8):1085-1091.
[11]余建兴.辣木油提取技术及对大鼠辅助降血脂作用的研究[D].昆明:昆明医学院,2009:49-57.
[12]FOTOUO-M H,DU TOIT E S,ROBBERTSE P J.Effect of storage conditions on Moringa oleifera Lam seed oil:Biodiesel feedstock quality[J].Industrial Crops and Products2016,84:80-86.
[13]虎虓真,陶宁萍,许长华.基于食药价值的辣木籽研究进展[J].食品科学,2018,39(15):302-309.
[14]高雅,李骅,王四旺,等.没食子酸的药理作用及其药物代谢动力学研究进展[J].西北药学杂志,2014,29(4):435-438.
[15]毕武.苦津茶抗肿瘤活性成分及槭属植物药用亲缘学研究[D].北京:北京协和医学院,2016.
[16]董成国.辣木籽水溶性多糖的分离纯化、结构表征及其抗氧化活性研究[D].哈尔滨:哈尔滨工业大学,2016.
[17]吕秋冰,向泽攀,徐向波,等.不同提取方式对辣木籽油品质影响分析[J].食品科技,2018,43(3):226-231.