车前子的利尿作用研究及发现
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摘要
目的:研究不同炮制规格的车前子的利尿作用。方法:采用大鼠水负荷模型,分别用不同浓度、不同炮制规格的车前子80%乙醇提取物给药,测定各组大鼠尿中总盐量排泄量、血尿素氮浓度和碳酸酐酶水平,进一步分析比较各提取物的利尿作用。结果:各炮制规格车前子利尿作用强弱:盐炙>清炒>生品;与空白对照组相比,高剂量生车前子醇提物尿素氮浓度有显著差异(P <0. 05),低剂量盐炙车前子醇提物碳酸酐水平有显著差异(P <0. 05);不同炮制规格高剂量车前子醇提物均出现腹泻情况。结论:各炮制规格的车前子均有明显的利尿作用,但剂量过高容易导致腹泻。
Objective: Study on diuretic effect of different processing specifications of Semen Plantaginis. Methods: Rats in water-loaded were administrated with different concentrations and processing specifications of alcohol extract of Semen Plantaginis,and total salt excretion in the urine,blood urea nitrogen concentration and carbonic anhydrase level in each group were determined.The diuretic effect of each extract was further analyzed and compared. Results: The diuretic effect of each preparation specifications: salt > stir-fried > raw; Compared with the blank control group,there was a significant difference in the concentration of urea nitrogen in the ethanol extract of plantain at high dose( P < 0. 05),and a significant difference in the level of carbonic anhydride in the alcohol extract of plantain at low dose( P < 0. 05). Diarrhea occurred in high dose alcohol extract of semen plantaginis of different processing specifications. Conclusion: All the prepared seeds have obvious diuretic effect,but too high dose can easily cause diarrhea.
Objective: Study on diuretic effect of different processing specifications of Semen Plantaginis. Methods: Rats in water-loaded were administrated with different concentrations and processing specifications of alcohol extract of Semen Plantaginis,and total salt excretion in the urine,blood urea nitrogen concentration and carbonic anhydrase level in each group were determined.The diuretic effect of each extract was further analyzed and compared. Results: The diuretic effect of each preparation specifications: salt > stir-fried > raw; Compared with the blank control group,there was a significant difference in the concentration of urea nitrogen in the ethanol extract of plantain at high dose( P < 0. 05),and a significant difference in the level of carbonic anhydride in the alcohol extract of plantain at low dose( P < 0. 05). Diarrhea occurred in high dose alcohol extract of semen plantaginis of different processing specifications. Conclusion: All the prepared seeds have obvious diuretic effect,but too high dose can easily cause diarrhea.
引文
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[2]谢明,杨爽爽,王亮亮,等.中药车前子的研究进展[J].黑龙江医药,2015,28(03):474-476.
[3]李冲冲,龚苏晓,许浚,等.车前子化学成分与药理作用研究进展及质量标志物预测分析[J].中草药,2018,49(06):1233-1246.
[4]郝明芬,范颖,梁茂新.车前子潜在功能的发掘与利用[J].时珍国医国药,2015,26(10):2479-2481.
[5]王蕾,姚东云,马红梅,等.中药利尿药理实验动物筛选方法探讨[J].中国比较医学杂志,2006(11):694-696.
[6]陈静,孙云超,冉小库,等.白术利尿作用研究[J].中国现代中药,2016,18(05):563-567.
[7]颜升,曾金祥,毕莹,等.车前子提取物对正常大鼠利尿活性及肾脏水通道蛋白与离子通道的作用[J].中国医院药学杂志,2014,34(12):968-971.
[8]张丹,朱伟,余昕,等.车前子不同炮制品化学特征指纹图谱模式的比较[J].中国医院药学杂志,2014,34(06):456-459.
[9]张丹,何颖,周洁,等.车前子不同炮制品的止泻作用及星点设计-效应面优化法优选车前子炒制工艺的研究[J].中草药,2014,45(03):355-361.
[10]刘川玉,周劲刚,何洁,等.车前子不同炮制品对慢性功能性便秘的疗效[J].中国实验方剂学杂志,2011,17(16):259-261.
[11]王隶书,李明洋,宋柏林,等.车前子多糖研究进展[J].时珍国医国药,2012,23(02):460-462.