艾灸干预胃溃疡大鼠胃黏膜脑肠肽的动态表达
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摘要
目的:观察艾灸干预胃溃疡大鼠胃黏膜相关脑肠肽的动态表达,探讨艾灸干预胃溃疡的时效关系。方法:SD大鼠72只随机分为3批,各批分为正常组、模型组、胃经穴位组和非经穴位组(n=6)。采用70%乙醇灌胃复制胃溃疡大鼠模型。胃(非)经穴位组的大鼠进行艾灸1、4、7d(30min/d)。干预结束后于光镜下观察胃黏膜形态,RT-PCR测定胃黏膜增殖细胞核抗原(PCNA)及P物质(SP)表达。结果:与正常组比较,模型组大鼠胃黏膜溃疡灶明显,胃黏膜PCNA表达降低(P<0.05),SP表达升高(P<0.05);与模型组比较,干预1d后胃经穴位组大鼠胃黏膜PCNA表达升高(P<0.05),干预4d后胃经穴位组PCNA表达升高(P<0.05),SP降低(P<0.05);干预7d后各组大鼠胃黏膜PCNA及SP表达无显著差异。结论:艾灸胃经穴位能有效调节相关脑肠肽指标,促进胃黏膜损伤修复,并具有一定的时效关系。
Objective: To observe the effects of moxa-wool moxibustion on dynamic expression of brain-gut peptide in gastric mucosa of rats with gastric ulcer, and to explore the time effect relationship of moxa-wool moxibustion in treating gastric ulcer. Methods: Seventy-two SD rats were randomly divided into three batches. Each batches was divided into control group, model group, stomach meridian acupoint group and non acupoints group(n=6). Rats model of gastric ulcer were established by 70% ethanol intragastric administration. The stomach meridian acupoint group and non acupoint moxibustion group were treated with moxa-wool moxibustions in 1 d, 4 d, 7 d(30 min/d). After intervention, morphology of gastric mucosa was observed under light microscope. The expressions of proliferating cell nuclear antigen(PCNA) and substance P(SP) in gastric mucosa were measured by RT-PCR. Results:Compared with the control group, the gastric mucosal ulcer in the rats of model group was obvious, the expression of PCNA in the gastric mucosa singnificantly decreased(P<0.05), and the expression of SP singnificantly increased(P<0.05). Compared with the model group, the PCNA expression in gastric mucosa of stomach meridian acupoints group increased after moxa-wool moxibustion in 1 d(P<0.05). After moxa-wool moxibustion 4 days, the expression of PCNA in stomach meridian acupoints group was increased(P<0.05), and SP was decreased(P<0.05). After moxa-wool moxibustion 7 days, there was no significant difference in the expression of PCNA and SP in each group. Conclusion: Moxa-wool moxibustion on the stomach meridian acupoints could effectively regulate the related brain gut peptide index, promote the repair of gastric mucosal injury, and has certain time-effect relationship.
Objective: To observe the effects of moxa-wool moxibustion on dynamic expression of brain-gut peptide in gastric mucosa of rats with gastric ulcer, and to explore the time effect relationship of moxa-wool moxibustion in treating gastric ulcer. Methods: Seventy-two SD rats were randomly divided into three batches. Each batches was divided into control group, model group, stomach meridian acupoint group and non acupoints group(n=6). Rats model of gastric ulcer were established by 70% ethanol intragastric administration. The stomach meridian acupoint group and non acupoint moxibustion group were treated with moxa-wool moxibustions in 1 d, 4 d, 7 d(30 min/d). After intervention, morphology of gastric mucosa was observed under light microscope. The expressions of proliferating cell nuclear antigen(PCNA) and substance P(SP) in gastric mucosa were measured by RT-PCR. Results:Compared with the control group, the gastric mucosal ulcer in the rats of model group was obvious, the expression of PCNA in the gastric mucosa singnificantly decreased(P<0.05), and the expression of SP singnificantly increased(P<0.05). Compared with the model group, the PCNA expression in gastric mucosa of stomach meridian acupoints group increased after moxa-wool moxibustion in 1 d(P<0.05). After moxa-wool moxibustion 4 days, the expression of PCNA in stomach meridian acupoints group was increased(P<0.05), and SP was decreased(P<0.05). After moxa-wool moxibustion 7 days, there was no significant difference in the expression of PCNA and SP in each group. Conclusion: Moxa-wool moxibustion on the stomach meridian acupoints could effectively regulate the related brain gut peptide index, promote the repair of gastric mucosal injury, and has certain time-effect relationship.
引文
[1]徐小茹,刘畅,韩明娟,等.针灸治疗胃溃疡取穴及配伍规律分析.中华针灸电子杂志,2014,3(6):32-36
[2]张民英,李恒,翟夏,等.温胃舒颗粒联合艾灸对胃溃疡患者(脾胃虚寒型)血清NO、NOS表达水平的影响.中医药导报,2016,22(8):48-51
[3]龚安,杨宗保,施彬彬,等.艾灸对急性胃溃疡大鼠胃黏膜组织形态学和血流量的影响.中华中医药杂志,2014,29(6):2001-2003
[4]张泓,郭华,张雨辰,等.艾灸对脾虚胃溃疡模型大鼠胃组织表皮生长因子受体、磷酸化细胞外信号调节激酶的影响.针刺研究,2014,39(5):351-357
[5]邓雪,任路,李静,等.电针“肝俞”穴对抑郁型胃溃疡大鼠胃窦黏膜、下丘脑组织P物质和海马5-羟色胺的影响.针刺研究,2014,39(2):124-129
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[7]孙安会,谷捷,吴涛,等.四种常用实验麻醉药物对大鼠心血管系统的影响.中国实验动物学报,2016,24(2):120-126
[8]覃慧林,王爱玲,邓为,等.木瓜乙酸乙酯部位对小鼠急性胃溃疡的保护作用.中药药理与临床,2015,31(2):45-49
[9]刘琼,杨宗保,王晨光,等.艾灸“梁门”“足三里”穴对应激性胃溃疡大鼠胃黏膜细胞相关蛋白质磷酸化水平的影响.中医杂志,2014,55(24):2129-2133
[10]杨宗保,王晨光,龚安,等.艾灸调节急性胃溃疡大鼠胃黏膜细胞相关标志蛋白质表达的研究.中国针灸,2013,33(11):1017-1021
[11]刘建平,卜涛,白建乐,等.EGF、TGF-α及EGFR在大鼠胃溃疡自愈过程中的表达及意义.中国药理学通报,2003,19(10):1185-1188
[12]韦玉娜,张静,王文杰,等.胃炎I号对慢性萎缩性胃炎PCNA、Bcl-2和Bax表达的影响.世界华人消化杂志,2015(6):944-948
[13]Peng L,Xie Y,Wang C,et al.Moxibustion alleviates gastricprecancerous lesions lesions in rats by promoting cell apoptosisand inhibiting proliferation-relates oncogenes.African Journalof Traditional Complementary&Alternative Medicines,2017,14(2):148-160
[14]李晓红,梁媛,谢宇晴,等.逍遥散对慢性束缚应激肝郁脾虚证模型大鼠SP、VIP基因表达的影响.中国中医基础医学杂志,2014(7):901-903
[15]石宏,程斌,李江慧,等.肥大细胞和P物质参与急性胃黏膜损伤大鼠体表穴位的敏化过程.针刺研究,2010,35(5):323-329
[16]Zhao X,Cheng Q,Qian Y,et al.Insect tea attenuates hydrochloricacid and ethanol-induced mice acute gastric injury.Experimental&Therapeutic Medicine,2017,14(5):5135-5142
[2]张民英,李恒,翟夏,等.温胃舒颗粒联合艾灸对胃溃疡患者(脾胃虚寒型)血清NO、NOS表达水平的影响.中医药导报,2016,22(8):48-51
[3]龚安,杨宗保,施彬彬,等.艾灸对急性胃溃疡大鼠胃黏膜组织形态学和血流量的影响.中华中医药杂志,2014,29(6):2001-2003
[4]张泓,郭华,张雨辰,等.艾灸对脾虚胃溃疡模型大鼠胃组织表皮生长因子受体、磷酸化细胞外信号调节激酶的影响.针刺研究,2014,39(5):351-357
[5]邓雪,任路,李静,等.电针“肝俞”穴对抑郁型胃溃疡大鼠胃窦黏膜、下丘脑组织P物质和海马5-羟色胺的影响.针刺研究,2014,39(2):124-129
[6]Pradeepkumar Singh L,Kundu P,Ganguly K,et al.Novel role offamotidine in downregulation of matrix metalloproteinase-9 duringprotection of ethanol-induced acute gastric ulcer.Free RadicalBiology and Medicine,2007,43(2):289
[7]孙安会,谷捷,吴涛,等.四种常用实验麻醉药物对大鼠心血管系统的影响.中国实验动物学报,2016,24(2):120-126
[8]覃慧林,王爱玲,邓为,等.木瓜乙酸乙酯部位对小鼠急性胃溃疡的保护作用.中药药理与临床,2015,31(2):45-49
[9]刘琼,杨宗保,王晨光,等.艾灸“梁门”“足三里”穴对应激性胃溃疡大鼠胃黏膜细胞相关蛋白质磷酸化水平的影响.中医杂志,2014,55(24):2129-2133
[10]杨宗保,王晨光,龚安,等.艾灸调节急性胃溃疡大鼠胃黏膜细胞相关标志蛋白质表达的研究.中国针灸,2013,33(11):1017-1021
[11]刘建平,卜涛,白建乐,等.EGF、TGF-α及EGFR在大鼠胃溃疡自愈过程中的表达及意义.中国药理学通报,2003,19(10):1185-1188
[12]韦玉娜,张静,王文杰,等.胃炎I号对慢性萎缩性胃炎PCNA、Bcl-2和Bax表达的影响.世界华人消化杂志,2015(6):944-948
[13]Peng L,Xie Y,Wang C,et al.Moxibustion alleviates gastricprecancerous lesions lesions in rats by promoting cell apoptosisand inhibiting proliferation-relates oncogenes.African Journalof Traditional Complementary&Alternative Medicines,2017,14(2):148-160
[14]李晓红,梁媛,谢宇晴,等.逍遥散对慢性束缚应激肝郁脾虚证模型大鼠SP、VIP基因表达的影响.中国中医基础医学杂志,2014(7):901-903
[15]石宏,程斌,李江慧,等.肥大细胞和P物质参与急性胃黏膜损伤大鼠体表穴位的敏化过程.针刺研究,2010,35(5):323-329
[16]Zhao X,Cheng Q,Qian Y,et al.Insect tea attenuates hydrochloricacid and ethanol-induced mice acute gastric injury.Experimental&Therapeutic Medicine,2017,14(5):5135-5142