鄂西北地区重楼质量控制与品质评价研究
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摘要
目的了解鄂西北地区重楼药材的质量情况,建立科学合理的品质评价方法,为道地药材重楼在该地区品种选育与林下仿野生规范化种植提供技术支撑。方法通过DNA条形码技术进行重楼属植物的区分;通过测定重楼药材的水分、总灰分、酸不溶性灰分及8种重楼皂苷的含量对重楼药材进行质量控制与品质评价。结果 DNA条形码序列ITS2能够直观有效的区分重楼属植物;对29批不同产地的重楼药材进行质量综合评价,结果显示茅箭区马家河龙王寨重楼药材质量较好。结论鄂西北地区不同产地品种的重楼质量存在明显差异,不同产地品种的重楼皂苷含量不同,同一产地七叶一枝花和竹溪重楼皂苷含量较高,质量较好,可以作为仿野生种植品种。
Objective To determine the quality of Paris medicinal materials in Northwest Hubei province, to establish a scientific and reasonable quality evaluation method, and to provide technical support for the selection and breeding of authentic Paris in this area and standardized planting of linxia copy in the wild. Methods We differentiated Paris by DNA barcode techniques, evaluated the quality of Paris by determining the content of moisture, total ash, acid-insoluble ash and the content of 8 polyphyllin. Results DNA barcode sequence ITS2 visually and effective distinguished different Paris. The quality of 29 batches of Paris medicinal materials from different producing areas was evaluated comprehensively. Quality of Paris medicinal materials from Majiahe Longwang village in Maojian District was better. Conclusion There are obvious differences in the quality and content of polyphyllin of different areas in Northwest Hubei. Paris polyphylla Smith var.chinensis(Franch.)Hara and Paris qiliangensis H. Li et J. Yang has higer polyphyllin and better quality, and can be treated as imitation of wild planting varieties.
Objective To determine the quality of Paris medicinal materials in Northwest Hubei province, to establish a scientific and reasonable quality evaluation method, and to provide technical support for the selection and breeding of authentic Paris in this area and standardized planting of linxia copy in the wild. Methods We differentiated Paris by DNA barcode techniques, evaluated the quality of Paris by determining the content of moisture, total ash, acid-insoluble ash and the content of 8 polyphyllin. Results DNA barcode sequence ITS2 visually and effective distinguished different Paris. The quality of 29 batches of Paris medicinal materials from different producing areas was evaluated comprehensively. Quality of Paris medicinal materials from Majiahe Longwang village in Maojian District was better. Conclusion There are obvious differences in the quality and content of polyphyllin of different areas in Northwest Hubei. Paris polyphylla Smith var.chinensis(Franch.)Hara and Paris qiliangensis H. Li et J. Yang has higer polyphyllin and better quality, and can be treated as imitation of wild planting varieties.
引文
[1]王跃虎,牛红梅,张兆云,等.重楼属植物的药用价值及其化学物质基础[J].中国中药杂志,2015,40(5):833-839.
[2]中国药典2015年版.一部[S]. 2015:260.
[3]卢伟,牟雄军,杨光义,等.中药重楼药理活性研究进展[J].中国药师,2017,20(5):896-899.
[4]洪燕,韩燕全,刘向国,等.重楼的质量控制及药理研究进展[J].山西中医学院学报,2013,14(6):66-69.
[5]黄贤校,高文远,满淑丽,等.重楼属药用植物皂苷类化学成分及其生源途径的研究进展[J].中草药,2009,40(3):483-489.
[6]冯丙江,徐淑媛.重楼的质量评价及其薯蓣皂苷元含量的测定[J].中国中医药科技,2012,19(5):432-433.
[7]李惠芬,平渊,陈刚,等.高效液相色谱法和薄层色谱法测定重楼中薯蓣皂苷元的含量[J].中草药,2003,34(2):127-129.
[8]黄端华,王凤英,童萍,等.气相色谱法测定重楼中薯蓣皂苷的水解产物[J].福州大学学报(自然科学版),2009,37(1):133-136.
[9]王维,叶强.顶空气相色谱法检测重楼提取物中大孔树脂残留物的量[J].海峡药学,2013,25(3):48-49.
[10]周浓,郭吉芬,杨丽云,等. HPLC测定不同采收期滇重楼中薯蓣皂苷元的含量[J].中国实验方剂学杂志,2010,16(18):54-56.
[11]徐丽丽,赵亮,夏晖,等. RP-HPLC法测定重楼中四种甾体皂苷的含量[J].药学实践杂志,2009,27(3):201-204.
[12]陈清,夏亚飞,阎姝. HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ,Ⅱ的含量[J].中国实验方剂学杂志,2012,18(6):78-80.
[13]王永慧,杨光义,叶方,等. UPLC法测定重楼药材中重楼皂昔Ⅰ和重楼皂昔Ⅱ的含量[J].中国药师,2012,15(11):1596-1597.
[14]韩燕全,洪燕,夏伦祝,等. UPLC-ELSD法同时测定重楼中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ[J].中草药,2012,43(2):305-307.
[15]洪淑华.重楼光谱和色谱指纹图谱研究[D].成都:西南交通大学,2009.
[16]郭婷,李焘,张自萍,等.七叶一枝花与滇重楼脂溶性成分的GC-MS分析[J].陕西农业科学,2011,57(4):14-16+114.
[17]王本伟,赵亮,张海,等.加速溶剂萃取-HPLC-TOF/MS法同时测定重楼中6种甾体皂苷类成分[J].第二军医大学学报,2012,33(5):549-552.
[18]何良艳,余美荣,陈建真,等.重楼的研究进展[J].现代中药研究与实践,2011,25(6):94-97.
[19]杨光义,胡培,叶方,等.重楼资源分布与可持续利用研究进展[J].中国药师,2016,19(1):159-162.
[20]陈吉炎,杨光义,安志斌,等.武当山地区中药资源品种调查研究初报[J].现代中药研究与实践,2010,24(6):19-21.
[21]陈吉炎,王雪芹,安志斌,等.武当山地区道地药材的发展模式研究[J].中国药房,2011,22(43):4123-4125.
[22]叶方,胡培,杨光义,等.湖北武当山地区重楼资源调查[J].中药材,2015,38(8):1615-1617.
[23]Yang G,Chen H,Zhao Z,et al. Resources and utilization of Taoist Medicinal Plants distributed in Wudang Mountain[J]. Journal of Chinese Pharmaceutical Sciences,2014,23(6):412-420.
[24]陈锡琨. HPLC测定宫血宁胶囊中重楼皂苷Ⅰ、Ⅱ、Ⅵ及Ⅶ的含量[J].中国现代应用药学,2013,30(12):1346-1349.
[25]张幼林,尚毅,叶方,等.武当山区市售重楼药材皂苷含量分析[J].中南药学,2017,15(8):1127-1130.
[26]叶方,黄良永,陈黎,等.武当山区人工种植重楼中9种重楼皂苷含量分析[J].中南药学,2016,14(11):1244-1248.
[27]郑江萍,程镇,叶方,等.武当山区重楼红外指纹图谱研究[J].儿科药学杂志,2016,22(10):47-50.
[28]黄芸,崔力剑,刘伟娜,等. HPLC-ELSD法分析重楼商品药材中甾体皂苷的含量[J].中国中药杂志,2006,31(15):1230-1233.
[2]中国药典2015年版.一部[S]. 2015:260.
[3]卢伟,牟雄军,杨光义,等.中药重楼药理活性研究进展[J].中国药师,2017,20(5):896-899.
[4]洪燕,韩燕全,刘向国,等.重楼的质量控制及药理研究进展[J].山西中医学院学报,2013,14(6):66-69.
[5]黄贤校,高文远,满淑丽,等.重楼属药用植物皂苷类化学成分及其生源途径的研究进展[J].中草药,2009,40(3):483-489.
[6]冯丙江,徐淑媛.重楼的质量评价及其薯蓣皂苷元含量的测定[J].中国中医药科技,2012,19(5):432-433.
[7]李惠芬,平渊,陈刚,等.高效液相色谱法和薄层色谱法测定重楼中薯蓣皂苷元的含量[J].中草药,2003,34(2):127-129.
[8]黄端华,王凤英,童萍,等.气相色谱法测定重楼中薯蓣皂苷的水解产物[J].福州大学学报(自然科学版),2009,37(1):133-136.
[9]王维,叶强.顶空气相色谱法检测重楼提取物中大孔树脂残留物的量[J].海峡药学,2013,25(3):48-49.
[10]周浓,郭吉芬,杨丽云,等. HPLC测定不同采收期滇重楼中薯蓣皂苷元的含量[J].中国实验方剂学杂志,2010,16(18):54-56.
[11]徐丽丽,赵亮,夏晖,等. RP-HPLC法测定重楼中四种甾体皂苷的含量[J].药学实践杂志,2009,27(3):201-204.
[12]陈清,夏亚飞,阎姝. HPLC比较醇提重楼及其颗粒中重楼皂苷Ⅰ,Ⅱ的含量[J].中国实验方剂学杂志,2012,18(6):78-80.
[13]王永慧,杨光义,叶方,等. UPLC法测定重楼药材中重楼皂昔Ⅰ和重楼皂昔Ⅱ的含量[J].中国药师,2012,15(11):1596-1597.
[14]韩燕全,洪燕,夏伦祝,等. UPLC-ELSD法同时测定重楼中重楼皂苷Ⅰ、Ⅱ、Ⅵ、Ⅶ[J].中草药,2012,43(2):305-307.
[15]洪淑华.重楼光谱和色谱指纹图谱研究[D].成都:西南交通大学,2009.
[16]郭婷,李焘,张自萍,等.七叶一枝花与滇重楼脂溶性成分的GC-MS分析[J].陕西农业科学,2011,57(4):14-16+114.
[17]王本伟,赵亮,张海,等.加速溶剂萃取-HPLC-TOF/MS法同时测定重楼中6种甾体皂苷类成分[J].第二军医大学学报,2012,33(5):549-552.
[18]何良艳,余美荣,陈建真,等.重楼的研究进展[J].现代中药研究与实践,2011,25(6):94-97.
[19]杨光义,胡培,叶方,等.重楼资源分布与可持续利用研究进展[J].中国药师,2016,19(1):159-162.
[20]陈吉炎,杨光义,安志斌,等.武当山地区中药资源品种调查研究初报[J].现代中药研究与实践,2010,24(6):19-21.
[21]陈吉炎,王雪芹,安志斌,等.武当山地区道地药材的发展模式研究[J].中国药房,2011,22(43):4123-4125.
[22]叶方,胡培,杨光义,等.湖北武当山地区重楼资源调查[J].中药材,2015,38(8):1615-1617.
[23]Yang G,Chen H,Zhao Z,et al. Resources and utilization of Taoist Medicinal Plants distributed in Wudang Mountain[J]. Journal of Chinese Pharmaceutical Sciences,2014,23(6):412-420.
[24]陈锡琨. HPLC测定宫血宁胶囊中重楼皂苷Ⅰ、Ⅱ、Ⅵ及Ⅶ的含量[J].中国现代应用药学,2013,30(12):1346-1349.
[25]张幼林,尚毅,叶方,等.武当山区市售重楼药材皂苷含量分析[J].中南药学,2017,15(8):1127-1130.
[26]叶方,黄良永,陈黎,等.武当山区人工种植重楼中9种重楼皂苷含量分析[J].中南药学,2016,14(11):1244-1248.
[27]郑江萍,程镇,叶方,等.武当山区重楼红外指纹图谱研究[J].儿科药学杂志,2016,22(10):47-50.
[28]黄芸,崔力剑,刘伟娜,等. HPLC-ELSD法分析重楼商品药材中甾体皂苷的含量[J].中国中药杂志,2006,31(15):1230-1233.