猪产气巴氏杆菌广东株的分离鉴定及其16S rDNA基因序列遗传进化分析
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摘要
产气巴氏杆菌是一种人兽共患性病原菌,可引起人和动物不同程度的流产、不孕等繁殖障碍性疾病。本文对广东某猪场采集的母猪产道分泌物进行病原分离检测,在产道拭子中分离到一种疑似巴氏杆菌的菌体。经细菌16S rDNA基因序列扩增、测序,鉴定为猪产气巴氏杆菌,命名为GD01株。序列分析显示GD01株与GenBank数据库中公布的产气巴氏杆菌16SrDNA核酸同源性为98.5%以上。本研究首次报道了猪产气巴氏杆菌在广东的流行,其在猪群的流行病学意义有待进一步调查研究。
引文
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[13]李富祥,李华春,杨仕标,宋建领,朱建波,廖德芳.一株尿素酶阴性产气巴氏杆菌的分离鉴定及生物学特性分析[J].中国预防兽医学报,39(3):240-243.
[2]Hommez J,Devriese LA.Pasteurella aerogenes isolations from swine[J].Zentralbl Veterinarmed B,1976,23(3):265-268.
[3]Kuhnert P,Heyberger-Meyer B,Nicolet J,Frey J.Characterization of PaxA and its operon:a cohemolytic RTX toxin determinant from pathogenic Pasteurella aerogenes[J].Infect Immun,2000,68(1):6-12.
[4]Kehrenberg C,Schwarz S.Molecular analysis of tetracycline resistance in Pasteurella aerogenes[J].Antimicrob Agents Chemot her,2001,45(10):2885-2890.
[5]Kehrenberg C,Schwarz S.Trimethoprim resistance in a porcine Pasteurella aerogenes isolate is based on a dfrA1 gene cassette located in a partially truncated class 2 integron[J].J Antimicrob Chemother,2011,66(2):450-452.
[6]Fodor L,Hajtós I,Glávits R.Abortion of a sow caused by Pasteurella aerogenes.Acta Vet Hung.1991;39(1-2):13-19.
[7]Lester A,Gerner-Smidt P,Gahrn-Hansen B,Sgaard P,Schmidt J,Frederiksen W.Phenotypical characters and ribotyping of Pasteurella aerogenes from different sources[J].Zentralbl Bakteriol.1993 Jun;279(1):75-82.
[8]Ejlertsen T,Gahrn-Hansen B,Sgaard P,Heltberg O,Frederiksen W.Pasteurella aerogenes isolated from ulcers or wounds in humans with occupational exposure to pigs:a report of 7 Danish cases[J].Scand J Infect Dis.1996;28(6):567-570.
[9]Quiles I,Blázquez JC,De Teresa L,Plaza J,Medrano C.Vertebral osteomyelitis due to Pasteurella aerogenes[J].Scand J Infect Dis.2000;32(5):566-567.
[10]Thorsen P,Moller BR,Arpi M,Bremmelgaard A,Frederiksen W.Pasteurella aerogenes isolated from stillbirth and mother[J].Lancet.1994,343(8895):485-486.
[11]Barnham M.Pig bite injuries and infection:report of seven human cases[J].Epidemiol Infect.1988,101(3):641-645.
[12]Thigpen JE,Clements ME,Gupta BN.Isolation of Pasteurella aerogenes from the uterus of a rabbit following abortion[J].Lab Anim Sci,1978,28(4):444-447.
[13]李富祥,李华春,杨仕标,宋建领,朱建波,廖德芳.一株尿素酶阴性产气巴氏杆菌的分离鉴定及生物学特性分析[J].中国预防兽医学报,39(3):240-243.