云南省德宏州烟草黑胫病菌对烯酰吗啉的敏感性
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摘要
为明确新开发烟叶生产区云南省德宏州烟草黑胫病菌对烯酰吗啉的敏感性现状,从德宏州13个县市采集68株烟草黑胫病菌株,采用菌丝生长速率法测定了烟草黑胫病菌株对烯酰吗啉的敏感性和抗药水平。结果表明,烯酰吗啉抑制烟草黑胫病菌菌丝生长的EC_(50)范围为0.462 8~2.575 2μg/m L,平均EC_(50)为1.388 1μg/m L,属于中抗水平。统计分析表明,烟草黑胫病菌68个菌株菌丝生长对烯酰吗啉的敏感性符合正态分布,最不敏感菌株的EC_(50)值为最敏感菌株的5.56倍。烯酰吗啉对德宏州烟草黑胫病仍有较高活性,但应与其他杀菌剂进行合理的轮换使用。
In order to clarify status quo of the sensitivity of tobacco black shank fungi to enoylmorpholine in the newly developed tobacco production area in Dehong Prefecture,Yunnan Province,the susceptibility was studied.68 strains of tobacco black shank were collected from 13 counties and cities of Dehong.The sensitivity and resistance level of the tobacco black shank strains to the alkenoylmorpholine were determined by the rate method.The results showed that the EC_(50) of mycelia growth was 0.462 8~2.575 2 μg/m L,and the average EC_(50) was 1.388 1 μg/m L,which belonged to the middle resistance level.The results also showed that the sensitivity of 68 mycelial growth to myoylmorpholine was in accordance with the normal distribution.EC_(50) of the least sensitive strains were 5.56 times of the most sensitive ones.Deacylmorpholine was still highly active to tobacco black shank in Dehong,but should be used with other fungicides for reasonable rotation.
In order to clarify status quo of the sensitivity of tobacco black shank fungi to enoylmorpholine in the newly developed tobacco production area in Dehong Prefecture,Yunnan Province,the susceptibility was studied.68 strains of tobacco black shank were collected from 13 counties and cities of Dehong.The sensitivity and resistance level of the tobacco black shank strains to the alkenoylmorpholine were determined by the rate method.The results showed that the EC_(50) of mycelia growth was 0.462 8~2.575 2 μg/m L,and the average EC_(50) was 1.388 1 μg/m L,which belonged to the middle resistance level.The results also showed that the sensitivity of 68 mycelial growth to myoylmorpholine was in accordance with the normal distribution.EC_(50) of the least sensitive strains were 5.56 times of the most sensitive ones.Deacylmorpholine was still highly active to tobacco black shank in Dehong,but should be used with other fungicides for reasonable rotation.
引文
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[11]汪汉成,李文红,李兴龙,等.不同类型杀菌剂对烟草黑胫病菌生物活性的研究[J].中国烟草学报,2012(4):46-50.
[12]战徊旭,温娜娜,罗定棋,等.泸州烟草黑胫病病原生理小种组成及对甲霜灵和烯酰吗啉的敏感性[J].植物保护,2015,41(6):178-184.
[13]苏凯,桑维钧,张新强,等.大理州烟草黑胫病菌对烯酰吗啉的敏感性测定[J].中国烟草科学,2015,36(1):102-106.
[14]苏凯,桑维钧,张新强,等.贵州省烟草黑胫病菌对烯酰吗啉的敏感性[J].植物保护,2013(1):154-158.
[15]朱桂宁,贤小勇,黄福,等.银法利和烯酰吗啉对烟草黑胫病菌的毒力测定及田间防治试验[J].西南农业学报,2011(6):2220-2224.
[16]Yang T P,Fu J G.A preliminary study on the resistance to different fungicides in Phytophthora parasitica var.nicotianae[J].Agricultural Science&Technology,2015,16(3):537-539.
[2]孙计平,李雪君,吴照辉,等.烟草黑胫病的研究进展[J].湖北农业科学,2011,50(16):3253-3256.
[3]徐兴阳,罗华元,欧阳进,等.红花大金元品种的烟叶质量特性及配套栽培技术探讨[J].中国烟草科学,2007,28(5):26-30.
[4]黄帮全,张芯丽.德宏烤烟生产和栽培节令适应性研究[J].热带农业科技,2015,38(4):39-41.
[5]唐旭兵,李光西,宋玉川,等.香料烟黑胫病病原鉴定及防治药剂筛选[J].中国烟草学报,2010,16(2):66-69.
[6]袁宗胜,张广民,刘延荣,等.烟草黑胫病菌对甲霜灵的敏感性测定[J].中国烟草科学,2001,22(4):9-12.
[7]汪汉成,李文红,冯勇刚,等.烟草黑胫病化学防治的历史与现状[J].中国烟草学报,2011(5):96-102.
[8]Gisi U,Sierotzki H.Fungicide modes of action and resistance in downy mildews[J].European Journal of Plant Pathology,2008,122:157-167.
[9]朱书生,卢晓红,陈磊,等.羧酸酰胺类(CAAs)杀菌剂研究进展[J].农药学学报,2010(1):1-12.
[10]王文桥,王丽,韩秀英,等.北方五省(区)马铃薯晚疫病菌对甲霜灵和精甲霜灵的敏感性检测[J].植物保护,2012(3):116-121.
[11]汪汉成,李文红,李兴龙,等.不同类型杀菌剂对烟草黑胫病菌生物活性的研究[J].中国烟草学报,2012(4):46-50.
[12]战徊旭,温娜娜,罗定棋,等.泸州烟草黑胫病病原生理小种组成及对甲霜灵和烯酰吗啉的敏感性[J].植物保护,2015,41(6):178-184.
[13]苏凯,桑维钧,张新强,等.大理州烟草黑胫病菌对烯酰吗啉的敏感性测定[J].中国烟草科学,2015,36(1):102-106.
[14]苏凯,桑维钧,张新强,等.贵州省烟草黑胫病菌对烯酰吗啉的敏感性[J].植物保护,2013(1):154-158.
[15]朱桂宁,贤小勇,黄福,等.银法利和烯酰吗啉对烟草黑胫病菌的毒力测定及田间防治试验[J].西南农业学报,2011(6):2220-2224.
[16]Yang T P,Fu J G.A preliminary study on the resistance to different fungicides in Phytophthora parasitica var.nicotianae[J].Agricultural Science&Technology,2015,16(3):537-539.