局部应用碘酊对兔游离皮瓣的影响
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摘要
目的构建兔皮下积液动物模型,探究质量分数2%碘酊对皮下积液及皮瓣愈合情况的影响。方法将32只新西兰兔随机分成A、B、C、D四组,A、B两组为对照组,C、D两组为处理组。处理组用2%碘酊处理游离皮瓣,对照组用生理盐水处理游离皮瓣。分别于术后3 d处死A、C组,术后5 d处死B、D组,检测体质量、白细胞、中性粒细胞百分比、皮下积液量,对游离皮瓣处组织进行组织病理学分析。结果处理组的皮下积液量少于对照组(P<0.05);胶原纤维增生占比及血管增生数量处理组均明显高于对照组(P<0.01)。体质量、白细胞计数及中性粒细胞百分比在四组间差异无统计学意义。结论 2%碘酊能促进兔游离皮瓣处胶原纤维及血管增生,减少皮下积液,加速创面愈合。
Objective To investigate the impact of local 2% iodine tincture application on healing wounding flap and reducing seroma formation following a model created in rabbits. Methods Thirtytwo New Zealand rabbits were randomly divided into groups A, B, C and D, group A and group B as control groups, group C and group D as treatment groups. The flaps of treatment groups were treated with 2% iodine tincture, and the control groups with normal saline. group A and group C were sacrificed on the third day after surgery, while group B and group D on the fifth day. We analyzed the results of weight, leukocyte count, neutrophil percentage, seroma volume of all groups and we evaluated tissue samples using histopathological examinations. Results Seroma formation was significantly decreased in groups C, D compared with groups A, B(P<0.05). The proportion of hyperplastic collagen fibrils and hyperplastic vessels number were both significantly higher in groups C, D than that of groups A, B(P<0.001). There is no significant difference among the four groups in terms of weight, leukocyte count and neutrophil percentage. Conclusion Two percent iodine tincture promoted angiogenesis and collagen synthesis of free flap, reduced seroma formation and accelerated wound healing of New Zealand rabbits.
Objective To investigate the impact of local 2% iodine tincture application on healing wounding flap and reducing seroma formation following a model created in rabbits. Methods Thirtytwo New Zealand rabbits were randomly divided into groups A, B, C and D, group A and group B as control groups, group C and group D as treatment groups. The flaps of treatment groups were treated with 2% iodine tincture, and the control groups with normal saline. group A and group C were sacrificed on the third day after surgery, while group B and group D on the fifth day. We analyzed the results of weight, leukocyte count, neutrophil percentage, seroma volume of all groups and we evaluated tissue samples using histopathological examinations. Results Seroma formation was significantly decreased in groups C, D compared with groups A, B(P<0.05). The proportion of hyperplastic collagen fibrils and hyperplastic vessels number were both significantly higher in groups C, D than that of groups A, B(P<0.001). There is no significant difference among the four groups in terms of weight, leukocyte count and neutrophil percentage. Conclusion Two percent iodine tincture promoted angiogenesis and collagen synthesis of free flap, reduced seroma formation and accelerated wound healing of New Zealand rabbits.
引文
[1]Desantis C,Ma J,Bryan L,et al.Breast cancer statistics,2013[J].CA Cancer J,2014,64(1):52-62.
[2]Ferlay J,Steliarova-Foucher E,Lortet-Tieulent J,et al.Reprint of:Cancer incidence and mortality patterns in Europe:Estimates for 40 countries in 2012[J].Eur J Cancer,2013,51(9):1374-1403.
[3]Faisal M,Abu-Elela ST,Mostafa W,et al.Efficacy of axillary exclusion on seroma formation after modified radical mastectomy[J].World J Surg Oncol,2016,14(1):234-240.
[4]Eser M,Tutal F,Kement M,et al.Effects of local phenytoin on seroma formation after mastectomy and Axillary lymph node dissection:an experimental study on mice[J].BMCSurg,2012,12(1):1-5.
[5]Turner EJ,Benson JR,Winters ZE.Techniques in the prevention and management of seromas after breast surgery[J].Future Oncol,2014,10(6):1049-1063.
[6]Van BJ,Theunissen LL,Snoeijs MG,et al.Flap fixation using tissue glue or sutures appears to reduce seroma aspiration after mastectomy for breast cancer[J].Clin Breast Cancer,2017,17(4):316-321.
[7]李岩.电视胸腔镜下单切口并2%浓碘酊胸膜固定治疗首次发作自发性气胸130例[J].中国实用医药,2012,7(26):25-26.
[8]王洪鲁,朱良明,孙志钢,等.胸腔镜碘酊胸膜固定术治疗恶性胸腔积液的临床研究[J].中国肿瘤临床,2015,42(3):167-172.
[9]Soares EWS,Nagai HM,Bredt LC,et al.Morbidity after conventional dissection of axillary lymph nodes in breast cancer patients[J].World J Surg Oncol,2014,12(1):1-8.
[10]Vasileiadou K,Kosmidis C,Anthimidis G,et al.Cyanoacrylate adhesive reduces seroma production after modified radical mastectomy or quadrantectomy with lymph node dissection?aa prospective randomized clinical trial[J].Clin Breast Cancer,2017,17(8):595-600.
[11]Khater A,Elnahas W,Roshdy S,et al.Evaluation of the quilting technique for reduction of postmastectomy seroma:a randomized controlled study.[J].Int J Breast Cancer,2015,2015(6):1-6.
[12]Kong D,Liu Y,Li Z,et al.OK-432(Sapylin)reduces seroma formation after axillary lymphadenectomy in breast cancer.[J].J Invest Surg,2016,30(1):1-5.
[13]Khan MA.Effect of preoperative intravenous steroids on seroma formation after modified radical mastectomy[J].JAyub Med Coll Abbottabad,2017,29(2):207-210.
[14]Bastelaar J V,Beckers A,Snoeijs M,et al.Flap fixation reduces seroma in patients undergoing mastectomy:a significant implication for clinical practice[J].World J Surg Oncol,2016,14(1):1-5.
[15]周英,张正伟.乳腺癌改良根治术后皮下积液40例分析[J].中华乳腺病杂志(电子版),2014,8(2):55-56.
[16]郭禹标,唐可京,杨惠玲,等.聚维酮碘诱导兔胸膜黏连的机制研究[J].中国病理生理杂志,2010,26(6):1161-1166.
[17]Garzagangemi AM,Barquetm u oz SA,Villarrealcolín SP,et al.Randomized phase II study of talc versus iodopovidone for the prevention of seroma formation following modified radical mastectomy.[J].Rev Invest Clin,2015,67(6):357-365.
[18]Re RN,Cook JL.An intracrine view of angiogenesis[J].Bioessays,2006,28(9):943-953.n~
[2]Ferlay J,Steliarova-Foucher E,Lortet-Tieulent J,et al.Reprint of:Cancer incidence and mortality patterns in Europe:Estimates for 40 countries in 2012[J].Eur J Cancer,2013,51(9):1374-1403.
[3]Faisal M,Abu-Elela ST,Mostafa W,et al.Efficacy of axillary exclusion on seroma formation after modified radical mastectomy[J].World J Surg Oncol,2016,14(1):234-240.
[4]Eser M,Tutal F,Kement M,et al.Effects of local phenytoin on seroma formation after mastectomy and Axillary lymph node dissection:an experimental study on mice[J].BMCSurg,2012,12(1):1-5.
[5]Turner EJ,Benson JR,Winters ZE.Techniques in the prevention and management of seromas after breast surgery[J].Future Oncol,2014,10(6):1049-1063.
[6]Van BJ,Theunissen LL,Snoeijs MG,et al.Flap fixation using tissue glue or sutures appears to reduce seroma aspiration after mastectomy for breast cancer[J].Clin Breast Cancer,2017,17(4):316-321.
[7]李岩.电视胸腔镜下单切口并2%浓碘酊胸膜固定治疗首次发作自发性气胸130例[J].中国实用医药,2012,7(26):25-26.
[8]王洪鲁,朱良明,孙志钢,等.胸腔镜碘酊胸膜固定术治疗恶性胸腔积液的临床研究[J].中国肿瘤临床,2015,42(3):167-172.
[9]Soares EWS,Nagai HM,Bredt LC,et al.Morbidity after conventional dissection of axillary lymph nodes in breast cancer patients[J].World J Surg Oncol,2014,12(1):1-8.
[10]Vasileiadou K,Kosmidis C,Anthimidis G,et al.Cyanoacrylate adhesive reduces seroma production after modified radical mastectomy or quadrantectomy with lymph node dissection?aa prospective randomized clinical trial[J].Clin Breast Cancer,2017,17(8):595-600.
[11]Khater A,Elnahas W,Roshdy S,et al.Evaluation of the quilting technique for reduction of postmastectomy seroma:a randomized controlled study.[J].Int J Breast Cancer,2015,2015(6):1-6.
[12]Kong D,Liu Y,Li Z,et al.OK-432(Sapylin)reduces seroma formation after axillary lymphadenectomy in breast cancer.[J].J Invest Surg,2016,30(1):1-5.
[13]Khan MA.Effect of preoperative intravenous steroids on seroma formation after modified radical mastectomy[J].JAyub Med Coll Abbottabad,2017,29(2):207-210.
[14]Bastelaar J V,Beckers A,Snoeijs M,et al.Flap fixation reduces seroma in patients undergoing mastectomy:a significant implication for clinical practice[J].World J Surg Oncol,2016,14(1):1-5.
[15]周英,张正伟.乳腺癌改良根治术后皮下积液40例分析[J].中华乳腺病杂志(电子版),2014,8(2):55-56.
[16]郭禹标,唐可京,杨惠玲,等.聚维酮碘诱导兔胸膜黏连的机制研究[J].中国病理生理杂志,2010,26(6):1161-1166.
[17]Garzagangemi AM,Barquetm u oz SA,Villarrealcolín SP,et al.Randomized phase II study of talc versus iodopovidone for the prevention of seroma formation following modified radical mastectomy.[J].Rev Invest Clin,2015,67(6):357-365.
[18]Re RN,Cook JL.An intracrine view of angiogenesis[J].Bioessays,2006,28(9):943-953.n~