HPLC测定三棱入血成分含量
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摘要
目的建立同时测定三棱入血成分对羟基苯甲酸、香草酸、阿魏酸和芦丁含量的方法。方法采用高效液相色谱法,Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm);流动相A为0.1%磷酸水溶液,B为甲醇,进行梯度洗脱;流速1.00 mL·min~(-1);检测波长254 nm;柱温为30℃。结果 4种成分分离度良好,且均在质量浓度0.000 5～0.25 mg·mL~(-1)内有良好的线性关系。各组含药血清样本的精密度RSD<10%,方法回收率>90%;各组样品稳定性RSD均<10%。表明本实验精密度良好,回收率和稳定性均符合药动学要求。结论该检测方法能够同时、快速、准确测定三棱4种有效成分在血清中的含量。
OBJECTIVE To establish a method for simultaneous determination of phydroxybenzoic acid, vanillic acid,ferulic acid and rutin absorbed into blood of Sparganii Rhizoma. METHODS The analysis was carried out by HPLC,chromatographic column: Agilent Eclipse XDB-C18(250 mm×4.6 mm, 5 μm); the mobile phase A was 0.1% phosphoric acid aqueous and solution B was methanol, and the gradient elution HPLC method was used; the flow rate was 1.00 mL·min~(-1); the detection wavelength was 254 nm; the column temperature was 30 ℃. RESULTS The four components showed good separation and good linear relationship in the concentration range of 0.000 5-0.25 mg·mL~(-1). The precision of serum sample in each group was good(RSD<10%), the recovery rate was >90%, and the stability of each group was good(RSD<10%), which satisfied the requirements of pharmacokinetics. CONCLUSION The method can determine the four active contents of Sparganii Rhizoma in serum simultaneously, accurately and rapidly.
OBJECTIVE To establish a method for simultaneous determination of phydroxybenzoic acid, vanillic acid,ferulic acid and rutin absorbed into blood of Sparganii Rhizoma. METHODS The analysis was carried out by HPLC,chromatographic column: Agilent Eclipse XDB-C18(250 mm×4.6 mm, 5 μm); the mobile phase A was 0.1% phosphoric acid aqueous and solution B was methanol, and the gradient elution HPLC method was used; the flow rate was 1.00 mL·min~(-1); the detection wavelength was 254 nm; the column temperature was 30 ℃. RESULTS The four components showed good separation and good linear relationship in the concentration range of 0.000 5-0.25 mg·mL~(-1). The precision of serum sample in each group was good(RSD<10%), the recovery rate was >90%, and the stability of each group was good(RSD<10%), which satisfied the requirements of pharmacokinetics. CONCLUSION The method can determine the four active contents of Sparganii Rhizoma in serum simultaneously, accurately and rapidly.
引文
[1]中国药典.一部[S].2015:13.
[2]YUAN T,HUA H M,FEI Y H.Chemical constituents of Sparganium stoloniferum(Ⅰ)[J].Chin Tradit Herb Drugs(中草药),2005,36(11):1607-1610.
[3]王新胜,吴启南,陈广云,等.三棱化学成分与质量评价的研究进展[J].中国药房,2013,24(15):1417-1420.
[4]FENG Y R,ZHANG W T,LI E W,et al.Research progress on chemical constituents and pharmacological activities of Sparganium stoloniferum[J].Chin Tradit Herb Drugs(中草药),2017,48(22):4804-4818.
[5]MA Y G,LI Q L.Study on the process for fabrication of Spargaii Rhizoma of the gas replacement[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(9):1066-1070.
[2]YUAN T,HUA H M,FEI Y H.Chemical constituents of Sparganium stoloniferum(Ⅰ)[J].Chin Tradit Herb Drugs(中草药),2005,36(11):1607-1610.
[3]王新胜,吴启南,陈广云,等.三棱化学成分与质量评价的研究进展[J].中国药房,2013,24(15):1417-1420.
[4]FENG Y R,ZHANG W T,LI E W,et al.Research progress on chemical constituents and pharmacological activities of Sparganium stoloniferum[J].Chin Tradit Herb Drugs(中草药),2017,48(22):4804-4818.
[5]MA Y G,LI Q L.Study on the process for fabrication of Spargaii Rhizoma of the gas replacement[J].Chin J Mod Appl Pharm(中国现代应用药学),2014,31(9):1066-1070.