痰热清吸入溶液的药效学评价
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摘要
目的:明确痰热清吸入溶液的镇咳、祛痰、解热及抗炎作用,为该制剂的进一步研发提供依据与数据支撑。方法:采用小鼠氨水引咳法及气管酚红排泌法分别观察痰热清吸入溶液的镇咳及祛痰作用;通过腹腔注射细菌脂多糖(LPS)建立大鼠发热模型,观察痰热清吸入溶液的解热作用;通过雾化吸入LPS建立大鼠急性肺炎模型,观察痰热清吸入溶液的抗炎作用。结果:痰热清吸入溶液可减少由氨水引发的小鼠咳嗽次数,增加小鼠气管酚红排泌量;降低LPS所致的大鼠体温及其相关调节因子前列腺素E2(PGE2)和环磷酸腺苷(c AMP)的水平;降低LPS所致大鼠急性肺炎支气管肺泡灌洗液(BALF)中白细胞(WBC)计数和中性粒细胞比例(NEUT),降低肺组织中核转录因子-κB(NF-κB)和白细胞介素-1β(IL-1β)的水平。结论:痰热清吸入溶液具有明显的镇咳、祛痰、解热及抗炎作用,值得进一步研发和推广。
Objective:To clarify the antitussive,expectorant,antipyretic and anti-inflammatory effects of Tanreqing inhalation solution,and provide basis and data support for further research and development of this preparation.Method:The methods of cough induced by ammonia and tracheal phenol red excretion were used to observe the antitussive and expectorant effects of Tanreqing inhalation solution in mice.The fever model of rats was established by intraperitoneal injection of bacterial lipopolysaccharide(LPS)to observe the antipyretic effect of the Tanreqing inhalation solution,the acute pneumonia model of rats was established by atomizing LPS inhalation,and the anti-inflammatory effect of Tanreqing inhalation solution was observed.Result:Tanreqing inhalation solution could reduce the number of coughs in mice induced by ammonia water,increase the amount of phenol red excretion in mouse trachea,decrease the levels of body temperature and its related regulatory factors of prostaglandin E_2(PGE_2)and cyclic adenosine monophosphate(cAMP)of rats induced by LPS,decrease the white blood cell(WBC)count and the neutrophil ratio(NEUT)in bronchoalveolar lavage fluid(BALF)of rats with LPS-induced acute pneumonia,and reduce the levels of nuclear transcription factor-κB(NF-κB)and interleukin-1β(IL-1β)in lung tissue.Conclusion:Tanreqing inhalation solution has obvious antitussive,expectorant,antipyretic and anti-inflammatory effects,which is worthy of further development and promotion.
Objective:To clarify the antitussive,expectorant,antipyretic and anti-inflammatory effects of Tanreqing inhalation solution,and provide basis and data support for further research and development of this preparation.Method:The methods of cough induced by ammonia and tracheal phenol red excretion were used to observe the antitussive and expectorant effects of Tanreqing inhalation solution in mice.The fever model of rats was established by intraperitoneal injection of bacterial lipopolysaccharide(LPS)to observe the antipyretic effect of the Tanreqing inhalation solution,the acute pneumonia model of rats was established by atomizing LPS inhalation,and the anti-inflammatory effect of Tanreqing inhalation solution was observed.Result:Tanreqing inhalation solution could reduce the number of coughs in mice induced by ammonia water,increase the amount of phenol red excretion in mouse trachea,decrease the levels of body temperature and its related regulatory factors of prostaglandin E_2(PGE_2)and cyclic adenosine monophosphate(cAMP)of rats induced by LPS,decrease the white blood cell(WBC)count and the neutrophil ratio(NEUT)in bronchoalveolar lavage fluid(BALF)of rats with LPS-induced acute pneumonia,and reduce the levels of nuclear transcription factor-κB(NF-κB)and interleukin-1β(IL-1β)in lung tissue.Conclusion:Tanreqing inhalation solution has obvious antitussive,expectorant,antipyretic and anti-inflammatory effects,which is worthy of further development and promotion.
引文
[1]韩燕鸿,张荷.痰热清注射液治疗小儿上呼吸道感染的系统评价[J].中国实验方剂学杂志,2016,22(12):215-219.
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[5]中华医学会呼吸病学分会《雾化吸入疗法在呼吸疾病中的应用专家共识》制定专家组.雾化吸入疗法在呼吸疾病中的应用专家共识[J].中华医学杂志,2016,96(34):2696-2708.
[6]Vogelmeier C F,Criner G J,Martinez F J,et al. Global strategy for the diagnosis,management,and prevention of chronic obstructive lung disease 2017 report[J].Respirology,2017,22(3):575-601.
[7]申昆玲,邓力,李云珠,等.支气管舒张剂在儿童呼吸道常见疾病中应用的专家共识[J].临床儿科杂志,2015,33(4):373-379.
[8]李翠,聂其霞,杜学航,等.不同压缩式雾化器对痰热清吸入溶液体外沉积性能的影响[J].中国实验方剂学杂志,2018,24(13):12-16.
[9]孙俊生,黄震,彭敏,等.单侧迷走神经切断对小鼠咳嗽及相关神经肽蛋白表达的影响[J].中国医学前沿杂志:电子版,2018,10(3):22-24.
[10]徐叔云,卞如濂,陈修.药理实验方法学[M]. 3版.北京:人民卫生出版社,2001:1363.
[11]苏青,吴婷婷,黄雅兰,等.黄芩提取物制备过程中化学成分及药效的变化规律分析[J].中国实验方剂学杂志,2018,24(14):1-6.
[12]张亚平,张广平,苏萍,等.不同途径吸入脂多糖致大鼠急性肺炎模型的优选[J].中国实验方剂学杂志,2018,24(7):82-88.
[13]Victor V M,Rocha M,De la Fuente M. Immune cells:free radicals and antioxidants in sepsis[J]. Int Immunopharmacol,2004,4(3):327-347.
[14]Lennie T A,Hirvonen M D,Mc Carthy D O,et al. Fever and the acute elevation in whole-body thermogenesis induced by lateral hypothalamic lesions[J]. Physiol Behav,1995,58(2):237-243.
[15]Corbett S W,Kaufman L N,Keesey R E. Thermogenesis after lateral hypothalamic lesions:contributions of brown adipose tissue[J]. Am J Physiol,1988,255(Pt1):E708-E715.
[16]Bird J H,Biggs T C,King E V. Controversies in the management of acute tonsillitis:an evidence-based review[J]. Clin Otolaryngol,2015,39(6):368-374.
[2]梅娜,王景红,张婧,等. 141例痰热清注射液不良反应分析[J].中日友好医院学报,2017,31(4):236-238.
[3]许思华.痰热清注射液的不良反应分析[J].北方药学,2017,14(2):151-152.
[4]Heyder J. Deposition of inhaled particles in the human respiratory tract and consequences for regional targeting in respiratory drug delivery[J]. Proc Am Thorac Soc,2004,1(4):315-320.
[5]中华医学会呼吸病学分会《雾化吸入疗法在呼吸疾病中的应用专家共识》制定专家组.雾化吸入疗法在呼吸疾病中的应用专家共识[J].中华医学杂志,2016,96(34):2696-2708.
[6]Vogelmeier C F,Criner G J,Martinez F J,et al. Global strategy for the diagnosis,management,and prevention of chronic obstructive lung disease 2017 report[J].Respirology,2017,22(3):575-601.
[7]申昆玲,邓力,李云珠,等.支气管舒张剂在儿童呼吸道常见疾病中应用的专家共识[J].临床儿科杂志,2015,33(4):373-379.
[8]李翠,聂其霞,杜学航,等.不同压缩式雾化器对痰热清吸入溶液体外沉积性能的影响[J].中国实验方剂学杂志,2018,24(13):12-16.
[9]孙俊生,黄震,彭敏,等.单侧迷走神经切断对小鼠咳嗽及相关神经肽蛋白表达的影响[J].中国医学前沿杂志:电子版,2018,10(3):22-24.
[10]徐叔云,卞如濂,陈修.药理实验方法学[M]. 3版.北京:人民卫生出版社,2001:1363.
[11]苏青,吴婷婷,黄雅兰,等.黄芩提取物制备过程中化学成分及药效的变化规律分析[J].中国实验方剂学杂志,2018,24(14):1-6.
[12]张亚平,张广平,苏萍,等.不同途径吸入脂多糖致大鼠急性肺炎模型的优选[J].中国实验方剂学杂志,2018,24(7):82-88.
[13]Victor V M,Rocha M,De la Fuente M. Immune cells:free radicals and antioxidants in sepsis[J]. Int Immunopharmacol,2004,4(3):327-347.
[14]Lennie T A,Hirvonen M D,Mc Carthy D O,et al. Fever and the acute elevation in whole-body thermogenesis induced by lateral hypothalamic lesions[J]. Physiol Behav,1995,58(2):237-243.
[15]Corbett S W,Kaufman L N,Keesey R E. Thermogenesis after lateral hypothalamic lesions:contributions of brown adipose tissue[J]. Am J Physiol,1988,255(Pt1):E708-E715.
[16]Bird J H,Biggs T C,King E V. Controversies in the management of acute tonsillitis:an evidence-based review[J]. Clin Otolaryngol,2015,39(6):368-374.