一种辅助降脂保健食品功能性及机制研究
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摘要
探讨以罗布麻叶、山楂、决明子为主要成分的保健食品在辅助降脂方面的作用及机制。将罗布麻叶、山楂、决明子提取物进行工艺优化,制成罗布麻叶降脂胶囊,根据保健食品的评定办法,复制大鼠混合型高脂血症模型,观察混合型高脂血症大鼠体重、肝重变化及在30 d和45 d其血清中血脂变化情况;同时,利用蛋白免疫印迹(Western blot)手段,检测组织内含半胱氨酸的天冬氨酸蛋白水解酶(Caspase),包括了Caspase-9、Caspase-3、Cleave Caspase-3蛋白表达量。结果表明:经工艺优化后,浓缩后制备稠膏重49 g,加入12.6 g的糊精,干燥粉碎后重43.8 g,水分为3.04%,制成罗布麻叶降脂胶囊;罗布麻叶降脂胶囊可改善高脂血症引起的大鼠体重、肝重系数、血脂指标及组织形态异常。同时,罗布麻叶降脂胶囊可能通过调节Caspase家族蛋白表达水平,发挥改善混合型高脂血症作用。
The role and mechanism of health foods with Apocynum venetum,hawthorn and cassia as main components in assisting lipid-lowering was explored. Apocynum venetum,hawthom and cassia seed extracts were optimized to prepare Apocynum venetum lipid-lowering capsules. According to the evaluation method of health food,the rat mixed hyperlipidemia model was duplicated to observe the changes in body weight,liver weight and serum lipids of rats with hyperlipidemia at 30 and 45 days. Besides,Cysteine-containing aspartate proteolytic enzymes(Caspase),including Caspase-9,Caspase-3,and Cleave Caspase-3 protein expression,were detected by the method of Western blot. The results showed that after the process optimization,the concentrated paste was concentrated to a weight of 49 g,12.6 g of dextrin was added,and the weight was 43.8 g after drying and pulverization,and the water content was 3.04 %,and the Apocynum venetum lipid-lowering capsule was prepared. Capsules could improve the body weight,liver weight coefficient,blood lipid index and tissue morphology abnormalities induced by hyperlipidemia. At the same time,Apocynum venetum lipid-lowering capsule might play a role in improving mixed hyperlipidemia by regulating the expression level of Caspase family proteins.
The role and mechanism of health foods with Apocynum venetum,hawthorn and cassia as main components in assisting lipid-lowering was explored. Apocynum venetum,hawthom and cassia seed extracts were optimized to prepare Apocynum venetum lipid-lowering capsules. According to the evaluation method of health food,the rat mixed hyperlipidemia model was duplicated to observe the changes in body weight,liver weight and serum lipids of rats with hyperlipidemia at 30 and 45 days. Besides,Cysteine-containing aspartate proteolytic enzymes(Caspase),including Caspase-9,Caspase-3,and Cleave Caspase-3 protein expression,were detected by the method of Western blot. The results showed that after the process optimization,the concentrated paste was concentrated to a weight of 49 g,12.6 g of dextrin was added,and the weight was 43.8 g after drying and pulverization,and the water content was 3.04 %,and the Apocynum venetum lipid-lowering capsule was prepared. Capsules could improve the body weight,liver weight coefficient,blood lipid index and tissue morphology abnormalities induced by hyperlipidemia. At the same time,Apocynum venetum lipid-lowering capsule might play a role in improving mixed hyperlipidemia by regulating the expression level of Caspase family proteins.
引文
[1]刘娜,孟康,张秀景,等.大柴胡汤合桂枝茯苓丸治疗气滞痰阻血瘀型混合型高脂血症的疗效观察[J].中西医结合心血管病电子杂志,2016(4):42-43
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[10]雷朝福,吴小英,胡金菊.他汀类联合非诺贝特治疗混合型高脂血症和致动脉粥样硬化血脂异常的社区应用[J].北方药学,2016,13(11):22-23
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[12]Bairaktari E,Tzallas C.Comparison of the efficacy of atorvastatin and micronised fenofibrate in the treatment of mixed hyperlipidemia[J].J Cardiovasc Risk,1999,144(2):113-116
[13]孙保平,孙小飞.一种用于治疗高血压的中药组合物、口服制剂及其制备方法:105381267A[P].2016-03-09
[14]王振勤.罗布麻叶(茶)的研究进展[J].中国中药杂志,1991(4):250-252
[15]石晓,尚新彬,豆康宁,等.降血压营养保健型挂面工艺研究[J].农业机械,2011(14):107-109
[16]刘洪生,叶新兰,李会涛,等.罗布麻叶营养泡腾饮片及其制造工艺:101455339 A[P].2009-06-17
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[18]Ma Y X,Chen S Y.Observations on the anti-aging,antihypertensive and antihyperlipemic effect of Apocynum venetum leaf extract[J].Zhong xi yi jie he za zhi,1989,9(6):323,335-337
[19]Yang S Y.Effect of water-extracts of leaves of luobuma(Apocynum lancifolium)on hyperlipemia in mice[J].Zhong Yao Tong Bao,1986,11(6):50-52
[20]Awasthi V K,Mahdi F,Chander R,et al.Hypolipidemic Activity of Cassia tora Seeds in Hyperlipidemic Rats[J].Indian J Clin Biochem,2015,30(1):78-83
[21]Shao F,Gu L,Chen H,et al.Comparation of Hypolipidemic and Antioxidant Effects of Aqueous and Ethanol Extracts of Crataegus pinnatifida Fruit in High-Fat Emulsion-Induced Hyperlipidemia Rats[J].Pharmacognosy Magazine,2016,12(45):64-69
[22]Hao X L,Kang Y,Li J K,et al.Protective effects of hyperoside against H2O2-induced apoptosis in human umbilical vein endothelial cells[J].Molecular Medicine Reports,2016,14(1):399-405
[23]Zhao X,Wang Q,Qian Y,et al.Cassia tora L.(Jue-ming-zi)has anticancer activity in TCA8113 cells in vitro and exerts anti-metastatic effects in vivo[J].Oncology Letters,2013,5(3):1036-1042
[24]Swaminathan J K,Khan M,Mohan I K,et al.Cardioprotective properties of Crataegus oxycantha extract against ischemia-reperfusion injury[J].Phytomedicine,2010,17(10):744-752
[2]禹振霞.阿昔莫司胶囊联合绞股蓝总甙片治疗以甘油三酯升高为主的混合型高脂血症的疗效观察[J].中国实用医药,2017,12(2):93-95
[3]刘冬立,王文利,郑宏,等.复方降脂茶治疗原发性高脂血症(痰浊内阻证)的临床研究[J].北京中医,2006,25(9):523-526
[4]Gao H T,Cheng W Z,Xu Q,et al.Dietary restriction reduces blood lipids and ameliorates liver function of mice with hyperlipidemia[J].Journal of Huazhong University of Science and Technology(Medical Sciences),2017,37(1):79-86
[5]Rai S,Bhatnagar S.Hyperlipidemia,Disease Associations,and Top10 Potential Drug Targets:A Network View.[J].Omics-a Journal of Integrative Biology,2016,20(3):152-168
[6]浮吟梅,樊军浩.我国保健食品的研发现状及发展趋势研究[J].中国食物与营养,2009(6):37-40
[7]王晶,李冰,奚茜,等.我国2003年以来注册的降脂保健茶的现状分析[J].中国食物与营养,2016,22(11):76-80
[8]国食药监保化.关于印发抗氧化功能评价方法等9个保健功能评价方法的通知[EB/OL].(2012-04-23).http://www.sda.gov.cn/WS01/CL0847/71257.html
[9]王育徽.自拟降脂散治疗高脂血症的临床研究[D].广州:广州中医药大学,2010:3-4
[10]雷朝福,吴小英,胡金菊.他汀类联合非诺贝特治疗混合型高脂血症和致动脉粥样硬化血脂异常的社区应用[J].北方药学,2016,13(11):22-23
[11]Farnier M.Cerivastatin in the treatment of mixed hyperlipidemia:The RIGHT study[J].American Journal of Cardiology,1998,82(4B):47J-51J
[12]Bairaktari E,Tzallas C.Comparison of the efficacy of atorvastatin and micronised fenofibrate in the treatment of mixed hyperlipidemia[J].J Cardiovasc Risk,1999,144(2):113-116
[13]孙保平,孙小飞.一种用于治疗高血压的中药组合物、口服制剂及其制备方法:105381267A[P].2016-03-09
[14]王振勤.罗布麻叶(茶)的研究进展[J].中国中药杂志,1991(4):250-252
[15]石晓,尚新彬,豆康宁,等.降血压营养保健型挂面工艺研究[J].农业机械,2011(14):107-109
[16]刘洪生,叶新兰,李会涛,等.罗布麻叶营养泡腾饮片及其制造工艺:101455339 A[P].2009-06-17
[17]龚建平,杨世林,晖简,等.降压降脂的复方罗布麻叶固体制剂及其制备方法:101125164A[P].2008-02-20
[18]Ma Y X,Chen S Y.Observations on the anti-aging,antihypertensive and antihyperlipemic effect of Apocynum venetum leaf extract[J].Zhong xi yi jie he za zhi,1989,9(6):323,335-337
[19]Yang S Y.Effect of water-extracts of leaves of luobuma(Apocynum lancifolium)on hyperlipemia in mice[J].Zhong Yao Tong Bao,1986,11(6):50-52
[20]Awasthi V K,Mahdi F,Chander R,et al.Hypolipidemic Activity of Cassia tora Seeds in Hyperlipidemic Rats[J].Indian J Clin Biochem,2015,30(1):78-83
[21]Shao F,Gu L,Chen H,et al.Comparation of Hypolipidemic and Antioxidant Effects of Aqueous and Ethanol Extracts of Crataegus pinnatifida Fruit in High-Fat Emulsion-Induced Hyperlipidemia Rats[J].Pharmacognosy Magazine,2016,12(45):64-69
[22]Hao X L,Kang Y,Li J K,et al.Protective effects of hyperoside against H2O2-induced apoptosis in human umbilical vein endothelial cells[J].Molecular Medicine Reports,2016,14(1):399-405
[23]Zhao X,Wang Q,Qian Y,et al.Cassia tora L.(Jue-ming-zi)has anticancer activity in TCA8113 cells in vitro and exerts anti-metastatic effects in vivo[J].Oncology Letters,2013,5(3):1036-1042
[24]Swaminathan J K,Khan M,Mohan I K,et al.Cardioprotective properties of Crataegus oxycantha extract against ischemia-reperfusion injury[J].Phytomedicine,2010,17(10):744-752