痹通冲剂中羟基红花黄色素A和葛根素的含量测定
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摘要
目的:建立同时测定痹通冲剂中羟基红花黄色素A、葛根素2种成分含量的高效液相色谱法。方法:色谱柱为安捷伦C_(18)柱(250 mm×4.6 mm,5.0μm);流动相为乙腈(A)和0.1%磷酸水溶液(B),梯度洗脱,流速为1.0 ml/min;柱温为35℃;检测波长为253 nm。结果:羟基红花黄色素A、葛根素含量分别在19.45～97.25μg/ml(r=0.999 96)、23.30～116.50μg/ml(r=0.999 98)范围内与峰面积呈良好的线性关系;羟基红花黄色素A的加样回收率为90.36%～103.6%,RSD为2.31%(n=9);葛根素的加样回收率为97.14%～109.24%,RSD为1.25%(n=9)。3批供试品中羟基红花黄色素A、葛根素的含量分别为0.858 6、1.460 5 mg/g。结论:本研究建立的方法简便、快速且准确,可用于痹通冲剂的质量控制。
OBJECTIVE: To establish the method for content determination of hydrosysafflor yellow A and puerarin at the same time in Bitong granules by high performance liquid chromatography(HPLC). METHODS: The chromatographic column was agilent C_(18)(250 mm×4.6 mm, 5 μm), the mobile phases was acetonitrile(A) and 0.1% aqueous phosphoric acid(B)(gradient elute), with flow rate of 1.0 ml/min, column temperature of 35 ℃, and detective wavelength of 253 nm. RESULTS: The calibration curve of hydroxysafflor yellow A was linear over the range of 19.45 μg/ml to 97.25 μg/ml(r=0.999 96), the calibration curve of puerarin was linear over the range of 23.30 μg/ml to 116.50 μg/ml(r=0.999 98). The recovery rate of hydroxysafflor yellow A was 90.36% to 103.6%, RSD was 2.31%(n=9), the recovery rate of puerarin was 97.14% to 109.24%, RSD was 1.25%(n=9). The average content of hydroxysafflor yellow A was 0.858 6 mg/g and the average content of puerarin was 1.460 5 mg/g in three batches of Bitong granules. CONCLUSIONS: The method established in this study is simple, rapid and accurate, and can be used for quality control of Bitong granules.
OBJECTIVE: To establish the method for content determination of hydrosysafflor yellow A and puerarin at the same time in Bitong granules by high performance liquid chromatography(HPLC). METHODS: The chromatographic column was agilent C_(18)(250 mm×4.6 mm, 5 μm), the mobile phases was acetonitrile(A) and 0.1% aqueous phosphoric acid(B)(gradient elute), with flow rate of 1.0 ml/min, column temperature of 35 ℃, and detective wavelength of 253 nm. RESULTS: The calibration curve of hydroxysafflor yellow A was linear over the range of 19.45 μg/ml to 97.25 μg/ml(r=0.999 96), the calibration curve of puerarin was linear over the range of 23.30 μg/ml to 116.50 μg/ml(r=0.999 98). The recovery rate of hydroxysafflor yellow A was 90.36% to 103.6%, RSD was 2.31%(n=9), the recovery rate of puerarin was 97.14% to 109.24%, RSD was 1.25%(n=9). The average content of hydroxysafflor yellow A was 0.858 6 mg/g and the average content of puerarin was 1.460 5 mg/g in three batches of Bitong granules. CONCLUSIONS: The method established in this study is simple, rapid and accurate, and can be used for quality control of Bitong granules.
引文
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[10]王福成,冯祥瑞,杨玲霞,等.HPLC法同时测定舒心宁片中羟基红花黄色素A、阿魏酸的含量.甘肃医药[J],2018,37(3):271-272.
[11]高风英.葛根素药理作用的研究进展[J].中草药,2003,34(12):附7-附8.
[12]潘丽丽.葛根素含量测定方法的研究进展[J].山东化工,2018,47(16):55-56.
[13]金丽,蔡培培,杭娟,等.铁必复颗粒质量标准研究[J].中国药业,2018,27(1):31-34.
[14]王腾宇,李德利,方明月,等.HPLC法测定金莲花汤中绿原酸、咖啡酸、葛根素、牡金素、迷迭香酸[J].药物评价研究,2018,41(6):1053-1056.
[15]杨金颖,翁蓓,孙芳芳.HPLC法测定回神颗粒中葛根素的含量[J].天津药学,2018,30(2):20-22.
[16]冯穗芬.HPLC法测定益气活血通络颗粒中葛根素的含量[J].中国民族民间医药,2018,27(5):17-19.
[2]国家药典委员会.中华人民共和国药典:一部[S].2015年版.北京:中国医药科技出版社,2015:151.
[3]李栋,马秉智,梁莹莹,等.羟基红花黄色素A的提取方法与含量测定研究进展[J].中日友好医院学报,2018,32(1):39-41.
[4]杨洋,李珍,丁华,等.蒙药制剂其格日木汤中羟基红花黄色素A含量的测定[J].中国民族医药杂志,2018,24(8):46-47.
[5]罗琴,自俊青,李智峰.HPLC测定二十五味大汤胶囊中的羟基红花黄色素A[J].华西药学杂志,2017,32(3):304-306.
[6]刘传玲,孙志义,高然,等.HPLC法测定红花跌打胶囊中羟基红花黄色素A的含量[J].药学研究,2017,36(4):206-208.
[7]袁常珍,梅刚.高效液相色谱法测定参麦散结胶囊中羟基红花黄色素A的含量[J].中国医院用药评价与分析,2017,17(3):377-378,384.
[8]彭宏伟,郭丽华.高效液相色谱法测定醋制消肿止痛液中苦参碱、氧化苦参碱、羟基红花黄色素A的含量[J].中国医院用药评价与分析,2017,17(8):1039-1041.
[9]戴作波,姜志戎.HPLC法测定麝香接骨丸中羟基红花黄色素A的含量[J].系统医学,2018,3(12):156-159.
[10]王福成,冯祥瑞,杨玲霞,等.HPLC法同时测定舒心宁片中羟基红花黄色素A、阿魏酸的含量.甘肃医药[J],2018,37(3):271-272.
[11]高风英.葛根素药理作用的研究进展[J].中草药,2003,34(12):附7-附8.
[12]潘丽丽.葛根素含量测定方法的研究进展[J].山东化工,2018,47(16):55-56.
[13]金丽,蔡培培,杭娟,等.铁必复颗粒质量标准研究[J].中国药业,2018,27(1):31-34.
[14]王腾宇,李德利,方明月,等.HPLC法测定金莲花汤中绿原酸、咖啡酸、葛根素、牡金素、迷迭香酸[J].药物评价研究,2018,41(6):1053-1056.
[15]杨金颖,翁蓓,孙芳芳.HPLC法测定回神颗粒中葛根素的含量[J].天津药学,2018,30(2):20-22.
[16]冯穗芬.HPLC法测定益气活血通络颗粒中葛根素的含量[J].中国民族民间医药,2018,27(5):17-19.