不同微生态制剂对海月水母生存与生长的影响
|
摘要
采用对比试验法,研究不同微生态制剂对海月水母生存与生长的影响。生存试验分为5组:Ⅰ组为对照组,饲喂基础水母配合饲料;Ⅱ组、Ⅲ组、Ⅳ组和Ⅴ组依次添加屎肠球菌、胶红酵母、沼泽红假单胞菌和EM10,结果显示:屎肠球菌和胶红酵母能在24 h内使海月水母严重受损,其余3组至72 h完好无损。生长试验分为4组,A组为对照组,B组~D组分别添加沼泽红假单胞菌、EM10和沼泽红假单胞菌与EM10的混合微生态制剂。每周测量水母伞径、收缩频率和pH值,NH_3-N、NO_2-N水平等指标。试验结果表明,沼泽红假单胞菌能促进海月水母的生长(P<0.05);EM10会抑制海月水母的生长(P<0.05);其与沼泽红假单胞菌的混合组水母生长性能介于两者之间(P>0.05),光合细菌是水母适宜的微生态制剂。
Two feeding trails was conducted to investigate the influence of different probiotics on the surviral and growth of the Moon jellyfish(Aurelia aurita). The Moon jellyfish were divided to five groups in the survival test, the control group Ⅰ was the fed basal formulated feed, the group Ⅱ was fed an Enterococcus faecium sp, the Rhodotorula mucilaginosa for group Ⅲ, the Rhodop seudanonas palustris for group Ⅳ and the EM10 for groupⅤ. After 24 h trial, the serious damage were found in the jellyfish of group Ⅱ and group Ⅲ, other groups were healthy. The growth experiment was consisted of four groups,the group A was a control, the group B was fed the Rhodop seudanonas palustris, the EM10 for group C and the mixture of Rhodop seudanonas palustris and EM10 was for group D. The swimming bell diameter and the expanding and contracting frequency, pH, NH_3-N, NO_2-N were detected every week. After three weeks trial, the result indicated that the Rhodop seudanonas palustris can increase the growth of the Moon jellyfish significantly(P<0.05), while the EM10 embodied the significant decreasing effect(P<0.05), the growth performance of mixture group was between the Rhodop seudanonas palustris' group and EM10 group. Therefore, the PSB Rhodop seudanonas palustris is suitable as probiotics for jellyfish Aurelia aurita.
Two feeding trails was conducted to investigate the influence of different probiotics on the surviral and growth of the Moon jellyfish(Aurelia aurita). The Moon jellyfish were divided to five groups in the survival test, the control group Ⅰ was the fed basal formulated feed, the group Ⅱ was fed an Enterococcus faecium sp, the Rhodotorula mucilaginosa for group Ⅲ, the Rhodop seudanonas palustris for group Ⅳ and the EM10 for groupⅤ. After 24 h trial, the serious damage were found in the jellyfish of group Ⅱ and group Ⅲ, other groups were healthy. The growth experiment was consisted of four groups,the group A was a control, the group B was fed the Rhodop seudanonas palustris, the EM10 for group C and the mixture of Rhodop seudanonas palustris and EM10 was for group D. The swimming bell diameter and the expanding and contracting frequency, pH, NH_3-N, NO_2-N were detected every week. After three weeks trial, the result indicated that the Rhodop seudanonas palustris can increase the growth of the Moon jellyfish significantly(P<0.05), while the EM10 embodied the significant decreasing effect(P<0.05), the growth performance of mixture group was between the Rhodop seudanonas palustris' group and EM10 group. Therefore, the PSB Rhodop seudanonas palustris is suitable as probiotics for jellyfish Aurelia aurita.
引文
[1]Chapman D M.Development of the tentacles and food groove in the jellyfish Aurelia aurita(Cnidaria:ScypHozoa)[J].Canadian Journal of Zoology,2001,79(4):623-632.
[2]Kui You,Caihua Ma,Huiwang Gao.Research on the jellyfish(Rhopilema esculentum Kishinouye)and associated aquaculture techniques in China:current status[J].Aquaculture International,2007,15:479-488.
[3]陈谦,张新雄,赵海,等.用于水产养殖的微生态制剂的研究和应用进展[J].应用与环境生物学报,2012,18(3):524-530.
[4]孟思妤,孟长明,陈昌福.水产用微生态制剂的应用现状与思考(上)[J].科学养鱼,2017(2):88.
[5]吕永辉,李明爽.我国水产养殖用微生态制剂行业现状与发展策略[J].中国水产,2015(8):34-36.
[6]Cathy H.Lucas.Reproduction and life history strategies of the common jellyfish,Aurelia aurita,in relation to its ambient environment[J].Hydrobiologia,2001,451:229-246.
[7]王文龙,冷向军,刘晃.不同饲料对海月水母生长性能的影响[J].大连水产学院学报,2009,24(5):412-416.
[8]顾明花.观赏性海月水母的调查及海月水母对氨氮的耐受性研究[D].上海:上海交通大学,2015:1-39.
[9]彭众,董丽,喻礼怀.屎肠球菌的作用机制及其在动物生产中应用的研究进展[J].中国饲料,2018,17:18-22.
[10]Wang Y,Tian Z,Yao J,et al.Effect of probiotics,Enterococcus faecium,on tilapia(Oreochromisniloticus)growth performance and immune response[J].Aquaculture,2008,277:203-207.
[11]马冬艳,吴维鹏,朱小明,等.贝类生态综合养殖系统中细菌丰度的变化[J].集美大学学报(自然科学版),2012,17(6):408-413.
[12]夏冬梅.胶红酵母对凡纳滨对虾、罗非鱼、卵形鲳鲹生长、消化酶活力和免疫的影响[D].上海:上海海洋大学,2014.
[13]孙锡凤,蒋万春.红酵母在动物生产中的应用研究进展[J].饲料研究,2014(15):1-5.
[14]Mackie G O,Meech RW,Spencer A N.A new inhibitory pathway in the jellyfish Polyorchis penicillatus[J].Canadian Journal of Zoology,2012,90:172-182.
[15]郭婉萍,赵晶,栾春雨,等.水产养殖用复合微生物粉剂中菌种的分子生物学鉴定[J].中国兽药杂志,2017,51(6):9-18
[16]孙盛明,苏艳莉,张武肖,等.饲料中添加枯草芽孢杆菌对团头鲂幼鱼生长性能、肝脏抗氧化指标、肠道菌群结构和抗病力的影响[J].动物营养学报,2016,28(2):507-514.
[17]李卫芬,邓斌,陈南南,等.芽孢杆菌对草鱼生长和肠黏膜抗氧化功能及养殖水质的影响[J].水生态学杂志,2012,33(1):65-70.
[18]宋奔奔,傅松哲,刘志培,等.水体中添加两种菌剂对凡纳滨对虾存活、生长及消化酶活力的影响[J].海洋科学,2009,4(33):1-5.
[19]赵剑闯,周勃.乳酸菌与芽孢杆菌对机体作用机制的比较[J].饲料与畜牧,2010(10):39-41.
[20]冯亚利.海洋光合细菌在水产养殖中的应用基础研究[D].哈尔滨:哈尔滨工业大学,2017.
[2]Kui You,Caihua Ma,Huiwang Gao.Research on the jellyfish(Rhopilema esculentum Kishinouye)and associated aquaculture techniques in China:current status[J].Aquaculture International,2007,15:479-488.
[3]陈谦,张新雄,赵海,等.用于水产养殖的微生态制剂的研究和应用进展[J].应用与环境生物学报,2012,18(3):524-530.
[4]孟思妤,孟长明,陈昌福.水产用微生态制剂的应用现状与思考(上)[J].科学养鱼,2017(2):88.
[5]吕永辉,李明爽.我国水产养殖用微生态制剂行业现状与发展策略[J].中国水产,2015(8):34-36.
[6]Cathy H.Lucas.Reproduction and life history strategies of the common jellyfish,Aurelia aurita,in relation to its ambient environment[J].Hydrobiologia,2001,451:229-246.
[7]王文龙,冷向军,刘晃.不同饲料对海月水母生长性能的影响[J].大连水产学院学报,2009,24(5):412-416.
[8]顾明花.观赏性海月水母的调查及海月水母对氨氮的耐受性研究[D].上海:上海交通大学,2015:1-39.
[9]彭众,董丽,喻礼怀.屎肠球菌的作用机制及其在动物生产中应用的研究进展[J].中国饲料,2018,17:18-22.
[10]Wang Y,Tian Z,Yao J,et al.Effect of probiotics,Enterococcus faecium,on tilapia(Oreochromisniloticus)growth performance and immune response[J].Aquaculture,2008,277:203-207.
[11]马冬艳,吴维鹏,朱小明,等.贝类生态综合养殖系统中细菌丰度的变化[J].集美大学学报(自然科学版),2012,17(6):408-413.
[12]夏冬梅.胶红酵母对凡纳滨对虾、罗非鱼、卵形鲳鲹生长、消化酶活力和免疫的影响[D].上海:上海海洋大学,2014.
[13]孙锡凤,蒋万春.红酵母在动物生产中的应用研究进展[J].饲料研究,2014(15):1-5.
[14]Mackie G O,Meech RW,Spencer A N.A new inhibitory pathway in the jellyfish Polyorchis penicillatus[J].Canadian Journal of Zoology,2012,90:172-182.
[15]郭婉萍,赵晶,栾春雨,等.水产养殖用复合微生物粉剂中菌种的分子生物学鉴定[J].中国兽药杂志,2017,51(6):9-18
[16]孙盛明,苏艳莉,张武肖,等.饲料中添加枯草芽孢杆菌对团头鲂幼鱼生长性能、肝脏抗氧化指标、肠道菌群结构和抗病力的影响[J].动物营养学报,2016,28(2):507-514.
[17]李卫芬,邓斌,陈南南,等.芽孢杆菌对草鱼生长和肠黏膜抗氧化功能及养殖水质的影响[J].水生态学杂志,2012,33(1):65-70.
[18]宋奔奔,傅松哲,刘志培,等.水体中添加两种菌剂对凡纳滨对虾存活、生长及消化酶活力的影响[J].海洋科学,2009,4(33):1-5.
[19]赵剑闯,周勃.乳酸菌与芽孢杆菌对机体作用机制的比较[J].饲料与畜牧,2010(10):39-41.
[20]冯亚利.海洋光合细菌在水产养殖中的应用基础研究[D].哈尔滨:哈尔滨工业大学,2017.