红球菌SY095产生物表面活性剂对沉积物中铅和铜的去除
|
摘要
采用批次淋洗的方法研究了红球菌SY095产生物表面活性剂对沉积物中Pb和Cu的去除作用,考察了生物表面活性剂浓度、pH、温度、振荡时间、提取次数等条件对重金属去除效果的影响,并分析处理前后重金属的形态变化。结果表明:随着生物表面活性剂浓度的增加,2种重金属的去除率增大;Pb在淋洗剂pH为6时解吸效果最好,达到34.16%,而Cu的去除效果随着pH的升高而增加;生物表面活性剂对沉积物Pb和Cu去除率分别在25,40℃时达到最大;随着振荡时间和提取次数的增加,Pb和Cu去除率升高,3次洗脱后累积去除率分别为39.12%和51.26%;对沉积物重金属的形态分析发现,生物表面活性剂可有效去除酸提取态Pb和Cu。
Batch leaching method was applied to investigate the removal efficiency of Pb and Cu from sediment by biosurfactant produced by Rhodococcus SY095, various factors such as biosurfactant concentration, pH, temperature, shaking time, extraction times, etc. were also studied to evaluate their influence on removal efficiency of heavy metals, and form transformation of heavy metals before and after leaching. The results showed that the removal rates of Pb and Cu increased following the increase of biosurfactant concentration; the best desorption efficiency of Pb was obtained at pH of 6.0, reaching 34.16%, while the desorption efficiency of Cu increased with increasing pH value; the maximum removal rates of Pb and Cu in sediments were obtained at 25 ℃ and 40 ℃ respectively; the removal rate of Pb and Cu increased with the increase of shaking time and extraction times, and the accumulative removal rates after three times of elution were 39.12% and 51.26% respectively; morphological analysis of heavy metals in sediments showed that biosurfactant could remove acid extractable Pb and Cu effectively.
Batch leaching method was applied to investigate the removal efficiency of Pb and Cu from sediment by biosurfactant produced by Rhodococcus SY095, various factors such as biosurfactant concentration, pH, temperature, shaking time, extraction times, etc. were also studied to evaluate their influence on removal efficiency of heavy metals, and form transformation of heavy metals before and after leaching. The results showed that the removal rates of Pb and Cu increased following the increase of biosurfactant concentration; the best desorption efficiency of Pb was obtained at pH of 6.0, reaching 34.16%, while the desorption efficiency of Cu increased with increasing pH value; the maximum removal rates of Pb and Cu in sediments were obtained at 25 ℃ and 40 ℃ respectively; the removal rate of Pb and Cu increased with the increase of shaking time and extraction times, and the accumulative removal rates after three times of elution were 39.12% and 51.26% respectively; morphological analysis of heavy metals in sediments showed that biosurfactant could remove acid extractable Pb and Cu effectively.
引文
[1] 国家海洋局.2017年中国海洋生态环境状况公报[EB/OL].http://www.soa.gov.cn/zwgk/hygb/zghyhjzlgb/201806/t201806 06_61389.html, 2018-6-06/2018-6-08.
[2] Maity J P, Huang Y M, Hsuc C M, et al. Removal of Cu, Pb and Zn by foam fractionation and a soil washing process from contaminated industrial soils using soapberry-derived saponin:a comparative effectiveness assessment[J]. Chemosphere, 2013, 92(10):1286-1293.
[3] 董汉英,仇荣亮,赵芝灏,等.工业废弃地多金属污染土壤组合淋洗修复技术研究[J].土壤学报,2010,47(6):1126-1133.
[4] 邓红侠,田鹏,丁克廉,等.不同淋洗剂对塿土中Cu、Pb的淋洗效果及对其他元素淋出的影响[J].环境化学,2017,36(6):1343-1352.
[5] 易龙生,王文燕,陶冶,等.有机酸对污染土壤重金属的淋洗效果研究[J].农业环境科学学报,2013,32(4):701-707.
[6] 丁宁,徐贝妮,彭灿,等.比较两种表面活性剂淋洗去除土壤中的重金属[J].环境工程学报,2017,11(11):6147-6154.
[7] Usman M M, Dadrasnia A, Kang T L, et al. Application of biosurfactants in environmental biotechnology: remediation of oil and heavy metal [J].AIMS Bioengineering, 2016, 3(3):289-304.
[8] 朱清清,邵超英,张琢,等.生物表面活性剂皂角苷增效去除土壤中重金属的研究[J]. 环境科学学报,2010,30(12):2491-2498.
[9] Ammami M T, Portet-Koltalo F, Benamar A, et al. Application of biosurfactants and periodic voltage gradient for enhanced electrokinetic remediation of metals and PAHs in dredged marine sediments[J].Chemosphere, 2015, 125: 1-8.
[10] Mulligan C N. Metal removal from contaminated soil and sediments by the biosurfactactant surfatactin[J]. Canadian Petroleum Technology Pollution, 1999, 21(3):3812-3820.
[11] 张斌,黄丽,张克强,等.皂角苷对几种生活污泥中Cu和Zn的去除[J].农业环境科学学报,2016,35(6):1180-1187.
[12] 张晓青,郝建安,杜瑾,等.海洋红球菌SY095产生物表面活性剂发酵培养基的优化[J].环境工程学报,2016,10(10):6065-6070.
[13] 张朝阳,彭平安,宋建中,等.改进BCR 法分析国家土壤标准物质中重金属化学形态[J].生态环境学报,2012,21(11):1881-1884.
[14] Miller R M. Biosurfactant-facilitated remediation of metal contaminated soils[C]//Conference on Fate, Traasport and Interactions of Metals. Tucson, Arizona, 1993, 4.
[15] 时进钢,袁兴中,曾光明,等.鼠李糖脂对沉积物中Cd 和Pb 的去除作用[J].环境化学,2005,24(1):55-58.
[16] 蓝梓铭,莫创荣,段秋实,等.鼠李糖脂对剩余污泥中铜和镍的去除[J].环境工程学报,2014, 8(3):1174-1178.
[17] Maity J P, Huang Y M, Hsu C M, et al. Removal of Cu, Pb and Zn by foam fractionation and a soil washing process from contaminated industrial soils using soapberry—derived saponin:a comparative effectiveness assessment[J].Chemsophere, 2013, 92(10):1286-1293.
[18] 张晓青,司晓光,郝建安,等.红球菌SY095产生物表面活性剂发酵工艺优化及产物稳定性研究[J].食品与发酵科技,2017,53(2):9-12.
[19] 李尤,廖晓勇,阎秀兰,等.鼠李糖脂淋洗修复重金属污染土壤的工艺条件优化研究[J]. 农业环境科学学报,2015,34(7):1287-1292.
[20] Alonso E, Aparicio I, Santos J L, et al. Sequential extraction of metals from mixed and digested sludge from aerobic WWTPs sited in the south of Spain[J]. Waste Management, 2009, 29(1):418-424.
[2] Maity J P, Huang Y M, Hsuc C M, et al. Removal of Cu, Pb and Zn by foam fractionation and a soil washing process from contaminated industrial soils using soapberry-derived saponin:a comparative effectiveness assessment[J]. Chemosphere, 2013, 92(10):1286-1293.
[3] 董汉英,仇荣亮,赵芝灏,等.工业废弃地多金属污染土壤组合淋洗修复技术研究[J].土壤学报,2010,47(6):1126-1133.
[4] 邓红侠,田鹏,丁克廉,等.不同淋洗剂对塿土中Cu、Pb的淋洗效果及对其他元素淋出的影响[J].环境化学,2017,36(6):1343-1352.
[5] 易龙生,王文燕,陶冶,等.有机酸对污染土壤重金属的淋洗效果研究[J].农业环境科学学报,2013,32(4):701-707.
[6] 丁宁,徐贝妮,彭灿,等.比较两种表面活性剂淋洗去除土壤中的重金属[J].环境工程学报,2017,11(11):6147-6154.
[7] Usman M M, Dadrasnia A, Kang T L, et al. Application of biosurfactants in environmental biotechnology: remediation of oil and heavy metal [J].AIMS Bioengineering, 2016, 3(3):289-304.
[8] 朱清清,邵超英,张琢,等.生物表面活性剂皂角苷增效去除土壤中重金属的研究[J]. 环境科学学报,2010,30(12):2491-2498.
[9] Ammami M T, Portet-Koltalo F, Benamar A, et al. Application of biosurfactants and periodic voltage gradient for enhanced electrokinetic remediation of metals and PAHs in dredged marine sediments[J].Chemosphere, 2015, 125: 1-8.
[10] Mulligan C N. Metal removal from contaminated soil and sediments by the biosurfactactant surfatactin[J]. Canadian Petroleum Technology Pollution, 1999, 21(3):3812-3820.
[11] 张斌,黄丽,张克强,等.皂角苷对几种生活污泥中Cu和Zn的去除[J].农业环境科学学报,2016,35(6):1180-1187.
[12] 张晓青,郝建安,杜瑾,等.海洋红球菌SY095产生物表面活性剂发酵培养基的优化[J].环境工程学报,2016,10(10):6065-6070.
[13] 张朝阳,彭平安,宋建中,等.改进BCR 法分析国家土壤标准物质中重金属化学形态[J].生态环境学报,2012,21(11):1881-1884.
[14] Miller R M. Biosurfactant-facilitated remediation of metal contaminated soils[C]//Conference on Fate, Traasport and Interactions of Metals. Tucson, Arizona, 1993, 4.
[15] 时进钢,袁兴中,曾光明,等.鼠李糖脂对沉积物中Cd 和Pb 的去除作用[J].环境化学,2005,24(1):55-58.
[16] 蓝梓铭,莫创荣,段秋实,等.鼠李糖脂对剩余污泥中铜和镍的去除[J].环境工程学报,2014, 8(3):1174-1178.
[17] Maity J P, Huang Y M, Hsu C M, et al. Removal of Cu, Pb and Zn by foam fractionation and a soil washing process from contaminated industrial soils using soapberry—derived saponin:a comparative effectiveness assessment[J].Chemsophere, 2013, 92(10):1286-1293.
[18] 张晓青,司晓光,郝建安,等.红球菌SY095产生物表面活性剂发酵工艺优化及产物稳定性研究[J].食品与发酵科技,2017,53(2):9-12.
[19] 李尤,廖晓勇,阎秀兰,等.鼠李糖脂淋洗修复重金属污染土壤的工艺条件优化研究[J]. 农业环境科学学报,2015,34(7):1287-1292.
[20] Alonso E, Aparicio I, Santos J L, et al. Sequential extraction of metals from mixed and digested sludge from aerobic WWTPs sited in the south of Spain[J]. Waste Management, 2009, 29(1):418-424.