蒙脱石改性前后对3种常见霉菌毒素的吸附效果及结构表征
|
摘要
本试验旨在研究蒙脱石经不同改性处理后对3种常见霉菌毒素的脱毒能力,以获得最佳的改性处理方法,并对蒙脱石改性前后结构进行表征。利用季铵盐及载金属离子对蒙脱石进行改性处理,测定改性前后对黄曲霉毒素B1(AFB1)、玉米赤霉烯酮(ZEN)和脱氧雪腐镰刀菌烯醇(DON)的吸附效果,并对蒙脱石原料及最佳改性处理后材料进行结构表征。结果表明:蒙脱石经6种改性处理后对3种霉菌毒素的吸附能力均显著增强(P<0.05),其中十八烷基三甲基氯化铵(STAC)改性处理后对AFB1、ZEN和DON吸附能力达到最大,分别为99.48%、93.62%和67.38%;结构表征结果显示,改性后蒙脱石的孔径、孔体积及比表面积均显著增加(P<0.05),红外光谱中出现STAC的特征峰,并检测到C、H和N元素。综上,利用载金属离子和季铵盐改性均能显著提高蒙脱石的脱毒能力,其中STAC改性是最佳改性方式;改性处理后蒙脱石分子结构疏松,STAC基团成功插层到蒙脱石分子结构中,更有利于加强对霉菌毒素的吸附能力。
The purpose of this trial was to study the effect of montmorillonite modified with different methods on the detoxification to three common mycotoxins, to screen the best modification methods, and to characterize the structure of montmorillonite before and after modification. The montmorillonite was modified with quaternary ammonium salt and cation-bearing. The adsorption capacity to aflatoxin B1(AFB1), zearalenone(ZEN) and deoxynivalenol(DON) were determined.In addition, the structure characterization was carried out. The results showed that the adsorption ability of montmorillonite to three mycotoxins was significantly enhanced(P<0.05) by six modification treatments. The adsorption to AFB1, ZEN and DON reached the maximum of 99.48%, 93.62% and 67.38%, which was obtained by STAC treatment. The results of structural characterization of montmorillonite showed that the pore size, pore volume and surface area were significantly increased(P<0.05). The characteristic peaks of STAC appeared, and C, H and N elements were detected in the modified montmorillonite. The above results showed that the modification of metal ions bearing and quaternary ammonium salt can significantly improve the detoxification ability of montmorillonite. The STAC treatment is the best method of modification.The structure of montmorillonite was loose after modification, and the STAC was successfully intercalated into the molecular structure, which is more conducive to strengthening its adsorption capacity to mycotoxin.
The purpose of this trial was to study the effect of montmorillonite modified with different methods on the detoxification to three common mycotoxins, to screen the best modification methods, and to characterize the structure of montmorillonite before and after modification. The montmorillonite was modified with quaternary ammonium salt and cation-bearing. The adsorption capacity to aflatoxin B1(AFB1), zearalenone(ZEN) and deoxynivalenol(DON) were determined.In addition, the structure characterization was carried out. The results showed that the adsorption ability of montmorillonite to three mycotoxins was significantly enhanced(P<0.05) by six modification treatments. The adsorption to AFB1, ZEN and DON reached the maximum of 99.48%, 93.62% and 67.38%, which was obtained by STAC treatment. The results of structural characterization of montmorillonite showed that the pore size, pore volume and surface area were significantly increased(P<0.05). The characteristic peaks of STAC appeared, and C, H and N elements were detected in the modified montmorillonite. The above results showed that the modification of metal ions bearing and quaternary ammonium salt can significantly improve the detoxification ability of montmorillonite. The STAC treatment is the best method of modification.The structure of montmorillonite was loose after modification, and the STAC was successfully intercalated into the molecular structure, which is more conducive to strengthening its adsorption capacity to mycotoxin.
引文
[1]CAST. Mycotoxins:Risk in plant, animal and human systems[J].AAHE-ERIC/Higher Education Res Rep, 2003, 9(7):48-50.
[2]Schatzmayr G, Streit E. Global occurrence of mycotoxins in thefood and feed chain:facts and figures[J]. World Mycotoxin J,2013, 6(3):213-222.
[3]Pedrosa K. Synergistic effect of mycotoxin contaminated feed[J].Int Pig Topics, 2010, 25(7):7-9.
[4]谢晓鹏,易卫,庄智明,等.饲料中的霉菌毒素及其防制措施[J].中国畜牧兽医, 2013, 40(5):101-106.
[5]Diaz D E, Smith T K. Mycotoxin sequestering agents:practicaltools for the neutralisation of mycotoxins[M].Thrumpton,Nottingham, UK:Nottingham University Press, 2005:323-339.
[6]Natale F D, Gallo M, Nigro R. Adsorbents selection for aflatoxinsremoval in bovine milks[J]. J Food Eng, 2009, 95(1):186-191.
[7]欧阳吉平,罗武辉,黄志强.超声/微波联合作用下双子季铵盐改性蒙脱石对苯酚的吸附特征[J].中国资源综合利用, 2017,35(6):8-11.
[8]Abdel-Wahhab M A, El-Nekeety A A, Hathout A S, et al.Preparation and characterization of organo-modified nanomontmorillonite and evaluation of its ability to adsorb aflatoxins,fumonisins and zearalenone from aqueous solution[J]. Arch Viro,2015, 48(3):271-274.
[9]Huwig A, Freimund S, K?ppeli O, et al. Mycotoxin detoxicationof animal feed by different adsorbents[J]. Toxicol Lett, 2001,122(2):179-188.
[10]齐云霞,夏伦志,吴东,等.蒙脱石对黄曲霉毒素B1吸附机制的研究进展[J].安徽农业科学, 2013(35):13606-13609.
[11]叶盛群,谌刚.几种脱毒剂对黄曲霉毒素B1和玉米赤霉烯酮体外吸附脱毒效果的比较研究[J].饲料工业, 2012, 33(11):28-30.
[12]Toma?evi?-?anovi?M, Dakovi?A, Rottinghaus G, et al.Surfactant modified zeolites––new efficient adsorbents formycotoxins[J]. Micropor Mesopor Mat, 2003, 61(1-3):173-180.
[13]Yu D Y, Song W H, Zhou B, et al. Assessment of Cu(II)-BearingMontmorillonite on Cd Adsorption[J]. Biol Trace Elem Res,2009, 130(2):185-192.
[14]李吉东,李玉宝,王学江,等.载铜锌纳米羟基磷灰石的抗菌性能及机理研究[J].无机材料学报, 2006, 21(1):162-168.
[15]Yang Y, Wang S, Liu J, et al. Adsor ption of lysine on Na-montmorillonite and competition with Ca2+:A Combined XRDand ATR-FTIR study[J]. Langmuir Acs J Sur Colloids, 2016,32(19):4746.
[16]Sing K S W, Williams R T. Physisorption Hysteresis Loops andthe Characterization of Nanoporous Materials[J]. Adsorpt SciTechnol, 2004, 22(10):773-782.
[17]陈雪刚,王丽丹,吕双双,等.季铵盐改性蒙脱石的超结构及其抗菌性能[J].浙江大学学报(工学版), 2010, 44(9):1831-1837.
[18]骆翼,陈峰.季铵盐处理蒙脱石的结构及其对玉米赤霉烯酮的吸附[J].饲料工业, 2014(s1):114-117.
[2]Schatzmayr G, Streit E. Global occurrence of mycotoxins in thefood and feed chain:facts and figures[J]. World Mycotoxin J,2013, 6(3):213-222.
[3]Pedrosa K. Synergistic effect of mycotoxin contaminated feed[J].Int Pig Topics, 2010, 25(7):7-9.
[4]谢晓鹏,易卫,庄智明,等.饲料中的霉菌毒素及其防制措施[J].中国畜牧兽医, 2013, 40(5):101-106.
[5]Diaz D E, Smith T K. Mycotoxin sequestering agents:practicaltools for the neutralisation of mycotoxins[M].Thrumpton,Nottingham, UK:Nottingham University Press, 2005:323-339.
[6]Natale F D, Gallo M, Nigro R. Adsorbents selection for aflatoxinsremoval in bovine milks[J]. J Food Eng, 2009, 95(1):186-191.
[7]欧阳吉平,罗武辉,黄志强.超声/微波联合作用下双子季铵盐改性蒙脱石对苯酚的吸附特征[J].中国资源综合利用, 2017,35(6):8-11.
[8]Abdel-Wahhab M A, El-Nekeety A A, Hathout A S, et al.Preparation and characterization of organo-modified nanomontmorillonite and evaluation of its ability to adsorb aflatoxins,fumonisins and zearalenone from aqueous solution[J]. Arch Viro,2015, 48(3):271-274.
[9]Huwig A, Freimund S, K?ppeli O, et al. Mycotoxin detoxicationof animal feed by different adsorbents[J]. Toxicol Lett, 2001,122(2):179-188.
[10]齐云霞,夏伦志,吴东,等.蒙脱石对黄曲霉毒素B1吸附机制的研究进展[J].安徽农业科学, 2013(35):13606-13609.
[11]叶盛群,谌刚.几种脱毒剂对黄曲霉毒素B1和玉米赤霉烯酮体外吸附脱毒效果的比较研究[J].饲料工业, 2012, 33(11):28-30.
[12]Toma?evi?-?anovi?M, Dakovi?A, Rottinghaus G, et al.Surfactant modified zeolites––new efficient adsorbents formycotoxins[J]. Micropor Mesopor Mat, 2003, 61(1-3):173-180.
[13]Yu D Y, Song W H, Zhou B, et al. Assessment of Cu(II)-BearingMontmorillonite on Cd Adsorption[J]. Biol Trace Elem Res,2009, 130(2):185-192.
[14]李吉东,李玉宝,王学江,等.载铜锌纳米羟基磷灰石的抗菌性能及机理研究[J].无机材料学报, 2006, 21(1):162-168.
[15]Yang Y, Wang S, Liu J, et al. Adsor ption of lysine on Na-montmorillonite and competition with Ca2+:A Combined XRDand ATR-FTIR study[J]. Langmuir Acs J Sur Colloids, 2016,32(19):4746.
[16]Sing K S W, Williams R T. Physisorption Hysteresis Loops andthe Characterization of Nanoporous Materials[J]. Adsorpt SciTechnol, 2004, 22(10):773-782.
[17]陈雪刚,王丽丹,吕双双,等.季铵盐改性蒙脱石的超结构及其抗菌性能[J].浙江大学学报(工学版), 2010, 44(9):1831-1837.
[18]骆翼,陈峰.季铵盐处理蒙脱石的结构及其对玉米赤霉烯酮的吸附[J].饲料工业, 2014(s1):114-117.