益生菌的功能及其在生猪养殖产业中的应用
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摘要
益生菌是一类微生物制剂,其代替抗生素作为新型饲料添加剂广泛应用于农业领域。本文结合国内外最新研究进展,综述了益生菌对猪肠道健康、营养利用、应激的影响以及在生猪养猪业的应用,旨在为益生菌在生猪养殖中的深度发掘及推广提供理论参考。
Probiotics is one kind of microbiological preparations,which is widely used a feed additive and alternative to antibiotics in the field of agriculture. Combined with the latest domestic and abroad research,this article reviews several aspects including the effects of probiotics on intestinal health,nutrient utilization and stress,and its application in pig breeding. The aim of this article is to provide theoretical reference for the deep excavation and application of probiotics a feed additive in pig breeding.
Probiotics is one kind of microbiological preparations,which is widely used a feed additive and alternative to antibiotics in the field of agriculture. Combined with the latest domestic and abroad research,this article reviews several aspects including the effects of probiotics on intestinal health,nutrient utilization and stress,and its application in pig breeding. The aim of this article is to provide theoretical reference for the deep excavation and application of probiotics a feed additive in pig breeding.
引文
[1]KONSTANTINOV S R,SMIDT H,AKKERMANS AD,et al.Feeding of Lactobacillus sobrius reduces Escherichia coli F4 levels in the gut and promotes grow th of infected piglets[J].FEM S M icrobiology E-cology,2008,66(3):599-607.
[2]HU S L,WANG L,JIANG Z Y.Dietary additive probiotics modulation of the intestinal microbiota[J].Protein&Peptide Letters,2017,24(5):382-387.
[3]SIGGERS R H,SIGGERS J,BOYE M,et al.Early administration of probiotics alters bacterial colonization and limits diet-induced gut dysfunction and severity of necrotizing enterocolitis in preterm pigs[J].The Journal of Nutrition,2008,138(8):1437-1444.
[4]LIU H,ZHANG J,ZHANG S H,et al.Oral administration of Lactobacillus fermentum I5007 favors intestinal development and alters the intestinal microbiota in formula-fed piglets[J].Journal of Agricultural and Food Chemistry,2014,62(4):860-866.
[5]DEMECKOVV,KELLY D,COUTTS A G P,et al.The effect of fermented liquid feeding on the faecal microbiology and colostrum quality of farrow ing sow s[J].International Journal of Food Microbiology,2002,79(1/2):85-97.
[6]MORAN C A,SCHOLTEN R H,TRICARICO J M,et al.Fermentation of w heat:effects of backslopping different proportions of pre-fermented w heat on the microbial and chemical composition[J].Archives of Animal Nutrition,2006,60(2):158-169.
[7]CASEY P G,GARDINER G E,CASEY G,et al.Afive-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged w ith salmonella enterica serovar Typhimurium[J].Applied and Environmental Microbiology,2007,73(6):1858-1863.
[8]QIAO J Y,LI H H,WANG Z X,et al.Effects of Lactobacillus acidophilus dietary supplementation on the performance,intestinal barrier function,rectal microflora and serum immune function in w eaned piglets challenged w ith Escherichia coli lipopolysaccharide[J].Antonie Van Leeuwenhoek,2015,107(4):883-891.
[9]PAJARILLO E A B,CHAE J P,BALOLONG M P,et al.Characterization of the fecal microbial communities of duroc pigs using 16S rRNA gene pyrosequencing[J].Asian-Australasian Journal of Animal Sciences,2015,28(4):584-591.
[10]BHMER B M,KRAMER W,ROTH-MAIER D A.Dietary probiotic supplementation and resulting effects on performance,health status,and microbial characteristics of primiparous sow s[J].Journal of Animal Physiology and Animal Nutrition,2006,90(7/8):309-315.
[11]TARAS D,VAHJEN W,MACHA M,et al.Performance,diarrhea incidence,and occurrence of Escherichia coli virulence genes during long-term administration of a probiotic Enterococcus faecium strain to sow s and piglets[J].Journal of Animal Science,2006,84(3):608-617.
[12]TARAS D,VAHJEN W,MACHA M,et al.Response of performance characteristics and fecal consistency to long-lasting dietary supplementation w ith the probiotic strain Bacillus cereus var.toyoi to sow s and piglets[J].Archives of Animal Nutrition,2005,59(6):405-417.
[13]CHE L Q,XU Q,WU C,et al.Effects of dietary live yeast supplementation on grow th performance,diarrhoea severity,intestinal permeability and immunological parameters of w eaned piglets challenged w ith enterotoxigenic Escherichia coli K88[J].British Journal of Nutrition,2017,118(11):949-958.
[14]张丽.新型酵母培养物的制备及其对断奶仔猪生长性能、表观消化率和粪便微生物的影响[D].硕士学位论文.北京:中国农业科学院,2016.
[15]DI GIANCAMILLO A,VITARI F,SAVOINI G,et al.Effects of orally administered probiotic Pediococcus acidilactici on the small and large intestine of w eaning piglets.A qualitative and quantitative micro-anatomical study[J].Histology and Histopathology,2008,23(6):651-664.
[16]REUNANEN J,KAINULAINEN V,HUUSKONENL,et al.Akkermansia muciniphila adheres to enterocytes and strengthens the integrity of the epithelial cell layer[J].Applied and Environmental M icrobiology,2015,81(11):3655-3662.
[17]CHAFFANEL F,CHARRON-BOURGOIN F,SOLI-GOT C,et al.Surface proteins involved in the adhesion of Streptococcus salivarius to human intestinal epithelial cells[J].Applied M icrobiology and Biotechnology,2018,102(6):2851-2865.
[18]CAZORLA S I,MALDONADO-GALDEANO C,WEILL R,et al.Oral administration of probiotics increases Paneth cells and intestinal antimicrobial activity[J].Frontiers in M icrobiology,2018,9:736.
[19]SZABI,WIELER L H,TEDIN K,et al.Influence of a probiotic strain of Enterococcus faecium on Salmonella enterica serovar typhimurium DT104 infection in a porcine animal infection model[J].Applied and Environmental M icrobiology,2009,75(9):2621-2628.
[20]RUIZ P A,HOFFMANN M,SZCESNY S,et al.Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats[J].Immunology,2005,115(4):441-450.
[21]ZANELLO G,BERRI M,DUPONT J,et al.Saccharomyces cerevisiae modulates immune gene expressions and inhibits ETEC-mediated ERK1/2 and p38 signaling pathw ays in intestinal epithelial cells[J].PLoSOne,2011,6(4):e18573.
[22]LEVINE B,KLIONSKY D J.Development by self-digestion:molecular mechanisms and biological functions of autophagy[J].Developmental Cell,2004,6(4):463-477.
[23]ZHANG L,XU Y Q,LIU H Y,et al.Evaluation of Lactobacillus rhamnosus GG using an Escherichia coli K88 model of piglet diarrhoea:effects on diarrhoea incidence,faecal microflora and immune responses[J].Veterinary M icrobiology,2010,141(1/2):142-148.
[24]SIRICHOKCHATCHAWAN W,PUPA P,PRAECHANSRI P,et al.Autochthonous lactic acid bacteria isolated from pig faeces in Thailand show probiotic properties and antibacterial activity against enteric pathogenic bacteria[J].M icrobial Pathogenesis,2018,119:208-215.
[25]LAN R X,LEE S I,KIM I H.Effects of multistrain probiotics on grow th performance,nutrient digestibility,blood profiles,faecal microbial shedding,faecal score and noxious gas emission in w eaning pigs[J].Journal of Animal Physiology and Animal Nutrition,2016,100(6):1130-1138.
[26]KREUZER S,JANCZYK P,ABMUS J,et al.No beneficial effects evident for Enterococcus faecium NCI-M B 10415 in w eaned pigs infected w ith Salmonella enterica serovar Typhimurium DT104[J].Applied and Environmental M icrobiology,2012,78(14):4816-4825.
[27]KIM E Y,KIM Y H,RHEE M H,et al.Selection of Lactobacillus sp.PSC101 that produces active dietary enzymes such as amylase,lipase,phytase and protease in pigs[J].The Journal of General and Applied M icrobiology,2007,53(2):111-117.
[28]LIU W C,DEVI S,PARK J,et al.Effects of complex probiotic supplementation in grow ing pig diets w ith and w ithout palm kernel expellers on grow th performance,nutrient digestibility,blood parameters,fecal microbial shedding and noxious gas emission[J].Animal Science Journal,2018,89(3):552-560.
[29]李方方,张慧,朱宇旌,等.微胶囊益生菌对肥育猪生长性能、血清生化指标及营养物质表观消化率的影响[J].养猪,2016(5):9-12.
[30]HUANG C H,QIAO S Y,LI D F,et al.Effects of Lactobacilli on the performance,diarrhea incidence,VFA concentration and gastrointestinal microbial flora of w eaning pigs[J].Asian-Australasian Journal of Animal Sciences,2004,17(3):401-409.
[31]史慧玲,杨福,郝晓鸣,等.不同的益生菌组合对保育猪粪污氮磷减排的影响[J].饲料研究,2015(10):24-27.
[32]LE BON M,DAVIES H E,GLYNN C,et al.Influence of probiotics on gut health in the w eaned pig[J].Livestock Science,2010,133(1/2/3):179-181.
[33]LV C H,WANG T,REGMI N,et al.Effects of dietary supplementation of selenium-enriched probiotics on production performance and intestinal microbiota of w eanling piglets raised under high ambient temperature[J].Journal of Animal Physiology and Animal Nutrition,2015,99(6):1161-1171.
[34]GAN F,REN F,CHEN X X,et al.Effects of selenium-enriched probiotics on heat shock protein mRNAlevels in piglet under heat stress conditions[J].Journal of Agricultural and Food Chemistry,2013,61(10):2385-2391.
[35]ALEXOPOULOS C,GEORGOULAKIS I E,TZI-VARA A,et al.Field evaluation of the efficacy of a probiotic containing Bacillus licheniformis and Bacillus subtilis spores,on the health status and performance of sow s and their litters[J].Journal of Animal Physiology and Animal Nutrition,2004,88(11/12):381-392.
[36]KHAFIPOUR E,MUNYAKA P M,NYACHOTI CM,et al.Effect of crow ding stress and Escherichia coli K88+challenge in nursery pigs supplemented w ith anti-Escherichia coli K88+probiotics[J].Journal of Animal Science,2014,92(5):2017-2029.
[37]MENG Q W,YAN L,AO X,et al.Influence of probiotics in different energy and nutrient density diets on grow th performance,nutrient digestibility,meat quality,and blood characteristics in grow ing-finishing pigs[J].Journal of Animal Science,2010,88(10):3320-3326.
[38]DAVIS M E,PARROTT T,BROWN D C,et al.Effect of a Bacillus-based direct-fed microbial feed supplement on grow th performance and pen cleaning characteristics of grow ing-finishing pigs[J].Journal of Animal Science,2008,86(6):1459-1467.
[39]呼红梅,林松,武英,等.饲粮添加益生菌对猪生长性能和肉品质的影响[J].养猪,2017(2):50-53.
[40]DOWARAH R,VERMA A K,AGARWAL N,et al.Efficacy of species-specific probiotic Pediococcus acidilactici FT28 on blood biochemical profile,carcass traits and physicochemical properties of meat in fattening pigs[J].Research in Veterinary Science,2018,117:60-64.
[41]SUO C,YIN Y S,WANG X N,et al.Effects of Lactobacillus plantarum ZJ316 on pig grow th and pork quality[J].BM C Veterinary Research,2012,8:89.
[42]ZEYNER A,BOLDT E.Effects of a probiotic Enterococcus faecium strain supplemented from birth to w eaning on diarrhoea patterns and performance of piglets[J].Journal of Animal Physiology and Animal Nutrition,2006,90(1/2):25-31.
[43]何佳,李慧,曾新福,等.复合益生菌对断奶仔猪生长性能、粪便微生物菌群和免疫功能的影响[J].饲料研究,2017(18):21-24.
[44]吴宁,杨加梅,张旭,等.中草药、益生菌、酸化剂联合添加替代抗生素在保育猪阶段的研究[J].饲料工业,2013(16):13-15.
[45]王艳,皮斌,张三军.益生菌发酵饲料对仔猪生长和免疫功能的影响[J].兽医导刊,2018(3):18-19.
[46]段明房,胡红伟,闫凌鹏,等.中草药益生菌复合制剂对生长猪血液常规、血清生化和血清免疫指标的影响[J].中国饲料,2018(3):54-59.
[47]JEONG J S,KIM I H.Effect of probiotic bacteria-fermented medicinal plants(G ynura procumbens,Rehmannia glutinosa,Scutellaria baicalensis)as performance enhancers in grow ing pigs[J].Animal Science Journal,2015,86(6):603-609.
[48]INATOMI T,AMATATSU M,ROMERO-PREZ GA,et al.Dietary probiotic compound improves reproductive performance of porcine epidemic diarrhea virus-infected sow s reared in a Japanese commercial sw ine farm under vaccine control condition[J].Front Immunol,2017,8:1877.
[2]HU S L,WANG L,JIANG Z Y.Dietary additive probiotics modulation of the intestinal microbiota[J].Protein&Peptide Letters,2017,24(5):382-387.
[3]SIGGERS R H,SIGGERS J,BOYE M,et al.Early administration of probiotics alters bacterial colonization and limits diet-induced gut dysfunction and severity of necrotizing enterocolitis in preterm pigs[J].The Journal of Nutrition,2008,138(8):1437-1444.
[4]LIU H,ZHANG J,ZHANG S H,et al.Oral administration of Lactobacillus fermentum I5007 favors intestinal development and alters the intestinal microbiota in formula-fed piglets[J].Journal of Agricultural and Food Chemistry,2014,62(4):860-866.
[5]DEMECKOVV,KELLY D,COUTTS A G P,et al.The effect of fermented liquid feeding on the faecal microbiology and colostrum quality of farrow ing sow s[J].International Journal of Food Microbiology,2002,79(1/2):85-97.
[6]MORAN C A,SCHOLTEN R H,TRICARICO J M,et al.Fermentation of w heat:effects of backslopping different proportions of pre-fermented w heat on the microbial and chemical composition[J].Archives of Animal Nutrition,2006,60(2):158-169.
[7]CASEY P G,GARDINER G E,CASEY G,et al.Afive-strain probiotic combination reduces pathogen shedding and alleviates disease signs in pigs challenged w ith salmonella enterica serovar Typhimurium[J].Applied and Environmental Microbiology,2007,73(6):1858-1863.
[8]QIAO J Y,LI H H,WANG Z X,et al.Effects of Lactobacillus acidophilus dietary supplementation on the performance,intestinal barrier function,rectal microflora and serum immune function in w eaned piglets challenged w ith Escherichia coli lipopolysaccharide[J].Antonie Van Leeuwenhoek,2015,107(4):883-891.
[9]PAJARILLO E A B,CHAE J P,BALOLONG M P,et al.Characterization of the fecal microbial communities of duroc pigs using 16S rRNA gene pyrosequencing[J].Asian-Australasian Journal of Animal Sciences,2015,28(4):584-591.
[10]BHMER B M,KRAMER W,ROTH-MAIER D A.Dietary probiotic supplementation and resulting effects on performance,health status,and microbial characteristics of primiparous sow s[J].Journal of Animal Physiology and Animal Nutrition,2006,90(7/8):309-315.
[11]TARAS D,VAHJEN W,MACHA M,et al.Performance,diarrhea incidence,and occurrence of Escherichia coli virulence genes during long-term administration of a probiotic Enterococcus faecium strain to sow s and piglets[J].Journal of Animal Science,2006,84(3):608-617.
[12]TARAS D,VAHJEN W,MACHA M,et al.Response of performance characteristics and fecal consistency to long-lasting dietary supplementation w ith the probiotic strain Bacillus cereus var.toyoi to sow s and piglets[J].Archives of Animal Nutrition,2005,59(6):405-417.
[13]CHE L Q,XU Q,WU C,et al.Effects of dietary live yeast supplementation on grow th performance,diarrhoea severity,intestinal permeability and immunological parameters of w eaned piglets challenged w ith enterotoxigenic Escherichia coli K88[J].British Journal of Nutrition,2017,118(11):949-958.
[14]张丽.新型酵母培养物的制备及其对断奶仔猪生长性能、表观消化率和粪便微生物的影响[D].硕士学位论文.北京:中国农业科学院,2016.
[15]DI GIANCAMILLO A,VITARI F,SAVOINI G,et al.Effects of orally administered probiotic Pediococcus acidilactici on the small and large intestine of w eaning piglets.A qualitative and quantitative micro-anatomical study[J].Histology and Histopathology,2008,23(6):651-664.
[16]REUNANEN J,KAINULAINEN V,HUUSKONENL,et al.Akkermansia muciniphila adheres to enterocytes and strengthens the integrity of the epithelial cell layer[J].Applied and Environmental M icrobiology,2015,81(11):3655-3662.
[17]CHAFFANEL F,CHARRON-BOURGOIN F,SOLI-GOT C,et al.Surface proteins involved in the adhesion of Streptococcus salivarius to human intestinal epithelial cells[J].Applied M icrobiology and Biotechnology,2018,102(6):2851-2865.
[18]CAZORLA S I,MALDONADO-GALDEANO C,WEILL R,et al.Oral administration of probiotics increases Paneth cells and intestinal antimicrobial activity[J].Frontiers in M icrobiology,2018,9:736.
[19]SZABI,WIELER L H,TEDIN K,et al.Influence of a probiotic strain of Enterococcus faecium on Salmonella enterica serovar typhimurium DT104 infection in a porcine animal infection model[J].Applied and Environmental M icrobiology,2009,75(9):2621-2628.
[20]RUIZ P A,HOFFMANN M,SZCESNY S,et al.Innate mechanisms for Bifidobacterium lactis to activate transient pro-inflammatory host responses in intestinal epithelial cells after the colonization of germ-free rats[J].Immunology,2005,115(4):441-450.
[21]ZANELLO G,BERRI M,DUPONT J,et al.Saccharomyces cerevisiae modulates immune gene expressions and inhibits ETEC-mediated ERK1/2 and p38 signaling pathw ays in intestinal epithelial cells[J].PLoSOne,2011,6(4):e18573.
[22]LEVINE B,KLIONSKY D J.Development by self-digestion:molecular mechanisms and biological functions of autophagy[J].Developmental Cell,2004,6(4):463-477.
[23]ZHANG L,XU Y Q,LIU H Y,et al.Evaluation of Lactobacillus rhamnosus GG using an Escherichia coli K88 model of piglet diarrhoea:effects on diarrhoea incidence,faecal microflora and immune responses[J].Veterinary M icrobiology,2010,141(1/2):142-148.
[24]SIRICHOKCHATCHAWAN W,PUPA P,PRAECHANSRI P,et al.Autochthonous lactic acid bacteria isolated from pig faeces in Thailand show probiotic properties and antibacterial activity against enteric pathogenic bacteria[J].M icrobial Pathogenesis,2018,119:208-215.
[25]LAN R X,LEE S I,KIM I H.Effects of multistrain probiotics on grow th performance,nutrient digestibility,blood profiles,faecal microbial shedding,faecal score and noxious gas emission in w eaning pigs[J].Journal of Animal Physiology and Animal Nutrition,2016,100(6):1130-1138.
[26]KREUZER S,JANCZYK P,ABMUS J,et al.No beneficial effects evident for Enterococcus faecium NCI-M B 10415 in w eaned pigs infected w ith Salmonella enterica serovar Typhimurium DT104[J].Applied and Environmental M icrobiology,2012,78(14):4816-4825.
[27]KIM E Y,KIM Y H,RHEE M H,et al.Selection of Lactobacillus sp.PSC101 that produces active dietary enzymes such as amylase,lipase,phytase and protease in pigs[J].The Journal of General and Applied M icrobiology,2007,53(2):111-117.
[28]LIU W C,DEVI S,PARK J,et al.Effects of complex probiotic supplementation in grow ing pig diets w ith and w ithout palm kernel expellers on grow th performance,nutrient digestibility,blood parameters,fecal microbial shedding and noxious gas emission[J].Animal Science Journal,2018,89(3):552-560.
[29]李方方,张慧,朱宇旌,等.微胶囊益生菌对肥育猪生长性能、血清生化指标及营养物质表观消化率的影响[J].养猪,2016(5):9-12.
[30]HUANG C H,QIAO S Y,LI D F,et al.Effects of Lactobacilli on the performance,diarrhea incidence,VFA concentration and gastrointestinal microbial flora of w eaning pigs[J].Asian-Australasian Journal of Animal Sciences,2004,17(3):401-409.
[31]史慧玲,杨福,郝晓鸣,等.不同的益生菌组合对保育猪粪污氮磷减排的影响[J].饲料研究,2015(10):24-27.
[32]LE BON M,DAVIES H E,GLYNN C,et al.Influence of probiotics on gut health in the w eaned pig[J].Livestock Science,2010,133(1/2/3):179-181.
[33]LV C H,WANG T,REGMI N,et al.Effects of dietary supplementation of selenium-enriched probiotics on production performance and intestinal microbiota of w eanling piglets raised under high ambient temperature[J].Journal of Animal Physiology and Animal Nutrition,2015,99(6):1161-1171.
[34]GAN F,REN F,CHEN X X,et al.Effects of selenium-enriched probiotics on heat shock protein mRNAlevels in piglet under heat stress conditions[J].Journal of Agricultural and Food Chemistry,2013,61(10):2385-2391.
[35]ALEXOPOULOS C,GEORGOULAKIS I E,TZI-VARA A,et al.Field evaluation of the efficacy of a probiotic containing Bacillus licheniformis and Bacillus subtilis spores,on the health status and performance of sow s and their litters[J].Journal of Animal Physiology and Animal Nutrition,2004,88(11/12):381-392.
[36]KHAFIPOUR E,MUNYAKA P M,NYACHOTI CM,et al.Effect of crow ding stress and Escherichia coli K88+challenge in nursery pigs supplemented w ith anti-Escherichia coli K88+probiotics[J].Journal of Animal Science,2014,92(5):2017-2029.
[37]MENG Q W,YAN L,AO X,et al.Influence of probiotics in different energy and nutrient density diets on grow th performance,nutrient digestibility,meat quality,and blood characteristics in grow ing-finishing pigs[J].Journal of Animal Science,2010,88(10):3320-3326.
[38]DAVIS M E,PARROTT T,BROWN D C,et al.Effect of a Bacillus-based direct-fed microbial feed supplement on grow th performance and pen cleaning characteristics of grow ing-finishing pigs[J].Journal of Animal Science,2008,86(6):1459-1467.
[39]呼红梅,林松,武英,等.饲粮添加益生菌对猪生长性能和肉品质的影响[J].养猪,2017(2):50-53.
[40]DOWARAH R,VERMA A K,AGARWAL N,et al.Efficacy of species-specific probiotic Pediococcus acidilactici FT28 on blood biochemical profile,carcass traits and physicochemical properties of meat in fattening pigs[J].Research in Veterinary Science,2018,117:60-64.
[41]SUO C,YIN Y S,WANG X N,et al.Effects of Lactobacillus plantarum ZJ316 on pig grow th and pork quality[J].BM C Veterinary Research,2012,8:89.
[42]ZEYNER A,BOLDT E.Effects of a probiotic Enterococcus faecium strain supplemented from birth to w eaning on diarrhoea patterns and performance of piglets[J].Journal of Animal Physiology and Animal Nutrition,2006,90(1/2):25-31.
[43]何佳,李慧,曾新福,等.复合益生菌对断奶仔猪生长性能、粪便微生物菌群和免疫功能的影响[J].饲料研究,2017(18):21-24.
[44]吴宁,杨加梅,张旭,等.中草药、益生菌、酸化剂联合添加替代抗生素在保育猪阶段的研究[J].饲料工业,2013(16):13-15.
[45]王艳,皮斌,张三军.益生菌发酵饲料对仔猪生长和免疫功能的影响[J].兽医导刊,2018(3):18-19.
[46]段明房,胡红伟,闫凌鹏,等.中草药益生菌复合制剂对生长猪血液常规、血清生化和血清免疫指标的影响[J].中国饲料,2018(3):54-59.
[47]JEONG J S,KIM I H.Effect of probiotic bacteria-fermented medicinal plants(G ynura procumbens,Rehmannia glutinosa,Scutellaria baicalensis)as performance enhancers in grow ing pigs[J].Animal Science Journal,2015,86(6):603-609.
[48]INATOMI T,AMATATSU M,ROMERO-PREZ GA,et al.Dietary probiotic compound improves reproductive performance of porcine epidemic diarrhea virus-infected sow s reared in a Japanese commercial sw ine farm under vaccine control condition[J].Front Immunol,2017,8:1877.