PRKAA1和UNC5CL基因位点多态性与胃癌的相关性研究
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摘要
目的:研究PRKAA1和UNC5CL基因位点多态性与胃癌发病的关系。方法:以我院2014年1月~2016年12月收治的90例胃癌患者为观察组,选择同期在我院进行治疗的90例胃炎患者作为对照组。提取分析患者的全血DNA样品,对其PRKAA1和UNC5CL基因位点基因分型进行检测,并进行组间比较。结果:(1)与对照组比较,观察组PRKAA1基因的rs13361707位点的CC型基因出现频率、rs10074991位点的GG基因出现频率、rs10036575位点的TT基因出现频率均显著高于对照组,rs10036575位点的CT+CC基因出现频率显著低于对照组,差异均具有统计学意义(P<0.05)。(2)观察组UNC5CL基因的rs2294693、rs742494位点的各基因分型的出现频率与对照组间比较差异无统计学意义(P>0.05)。结论:胃癌患者的发病与PRKAA1基因多态性表达有一定的相关性,当rs13361707位点为CC型、rs10074991位点为GG型、rs10036575位点为TT型时,胃癌的发病率增加,当rs10036575位点为CT+CC型时,胃癌发病率降低。而胃癌患者的发病与UNC5CL基因位点多态性表达无明显相关性。
Objective: To study the relationship between the polymorphism of PRKAA1 and UNC5CL gene loci and the pathogenesis of gastric cancer. Methods: 90 cases of patients with gastric cancer treated in our hospital from January 2014 to December~2016 were selected as the observation group, and 90 patients with gastritis treated in our hospital during the same period were selected as the control group. The DNA samples of blood samples were extracted and analyzed. The genotypes of PRKAA1 and UNC5CL loci were detected and compared between the two groups. Results:(1) The CC frequency of rs13361707 loci, GG frequency of rs10074991 loci and TT frequency of rs10036575 loci in the PRKAA1 gene of observation group were significantly higher than those of the control group(P<0.05), while the CT+CC frequency of rs10036575 loci in the PRKAA1 gene of observation group was significantly lower than that of the control group(P<0.05).(2) No significant difference was found in the frequency of rs2294693 and rs742494 loci in the UNC5CL gene between two groups(P>0.05). Conclusion: There is some correlation of the incidence of gastric cancer with the polymorphism of PRKAA1, the incidence rate of gastric cancer is increased in the patients with rs13361707 site CC, site of rs10074991 GG type gastric cancer, which was decreased in the rs10036575 site CT+CC type. There is no correlation of the expression of UNC5CL gene loci and the incidence of gastric cancer.
Objective: To study the relationship between the polymorphism of PRKAA1 and UNC5CL gene loci and the pathogenesis of gastric cancer. Methods: 90 cases of patients with gastric cancer treated in our hospital from January 2014 to December~2016 were selected as the observation group, and 90 patients with gastritis treated in our hospital during the same period were selected as the control group. The DNA samples of blood samples were extracted and analyzed. The genotypes of PRKAA1 and UNC5CL loci were detected and compared between the two groups. Results:(1) The CC frequency of rs13361707 loci, GG frequency of rs10074991 loci and TT frequency of rs10036575 loci in the PRKAA1 gene of observation group were significantly higher than those of the control group(P<0.05), while the CT+CC frequency of rs10036575 loci in the PRKAA1 gene of observation group was significantly lower than that of the control group(P<0.05).(2) No significant difference was found in the frequency of rs2294693 and rs742494 loci in the UNC5CL gene between two groups(P>0.05). Conclusion: There is some correlation of the incidence of gastric cancer with the polymorphism of PRKAA1, the incidence rate of gastric cancer is increased in the patients with rs13361707 site CC, site of rs10074991 GG type gastric cancer, which was decreased in the rs10036575 site CT+CC type. There is no correlation of the expression of UNC5CL gene loci and the incidence of gastric cancer.
引文
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[2]王倩,张小珍,王姣,等.中国人群ERCC5基因多态性与胃癌易感相关性研究的荟萃分析[J].临床检验杂志,2016,34(11):869-875Wang Qian,Zhang Xiaozhen,Wang Jiao,et al.Meta analysis of the association between ERCC5 gene polymorphisms and susceptibility to gastric cancer in Chinese population[J].Journal of clinical laboratory 2016,34(11):869-875
[3]Hu N,Wang Z,Song X,et al.Genome-wide association study of gastric adenocarcinoma in Asia:a comparison of associations between cardia and non-cardia tumours[J].Gut,2016,65(10):1611-1618
[4]方法,毕兴,高洁,等.新疆维吾尔族人群胃癌与维生素D受体基因FokⅠ多态性的相关性[J].世界华人消化杂志,2016,24(11):1734-1740Fang Fa,Bi Xing,Gao Jie,et al.Correlation between gastric cancer and vitamin D receptor gene Fok I polymorphism in Xinjiang Uygur population[J].World Chinese Journal of digestion,2016,24(11):1734-1740
[5]Eom S Y,Hong S M,Yim D H,et al.Additive interactions between PRKAA1polymorphisms and Helicobacter pylori Cag A infection associated with gastric cancer risk in Koreans[J].Cancer Medicine,2016,5(11):3236-3335
[6]Qiu L X,He J,Cheng L,et al.Genetic variant of PRKAA1 and gastric cancer risk in an eastern Chinese population[J].Oncotarget,2015,6(40):42661-42666
[7]李涛,赖春凤,丘波,等.CYP2E1基因多态性与客家人群胃癌易感性的研究[J].国际肿瘤学杂志,2016,43(7):495-498Li Tao,Lai Chun-feng,Qiu Bo,et al.Association of CYP2E1 gene polymorphisms with susceptibility to gastric cancer in Hakka population[J].International Journal of Oncology,2016,43(7):495-498
[8]高俊,邱秀娟,汤建军,等.mi RNA-196a2 rs116149131基因多态性与胃癌患病风险的Meta分析[J].临床肿瘤学杂志,2016,21(9):789-795Gao Jun,Qiu Xiu-juan,Tang Jian-jun,et al.Meta analysis of mi RNA-196a2 rs116149131 gene polymorphism and the risk of gastric cancer[J].Journal of Clinical Oncology,2016,21(9):789-795
[9]Yuan J,Zhang Y,Yan F T,et al.Association of PRKAA1,gene polymorphisms with chronic hepatitis B virus infection in Chinese Han population[J].Brazilian Journal of Infectious Diseases An Official Publication of the Brazilian Society of Infectious Diseases,2016,20(6):564-568
[10]张永菊,张春艳,于新娟,等.PRKAA1和UNC5CL基因多态性与胃癌易感性的关系[J].中国临床医生杂志,2016,44(10):21-25Zhang Yong-ju,Zhang Chun-yan,Yu Xin-juan,et al.In Xinjuan,PRKAA1 and UNC5CL gene polymorphisms and susceptibility to gastric cancer[J].Chinese clinicians magazine,2016,44(10):21-25
[11]吴迪.PSCA、PLCE1、PRKAA1基因多态性及幽门螺旋杆菌感染与胃癌易感性的关系[D].兰州大学,2014Wu Di.PSCA,PLCE1,PRKAA1 gene polymorphisms and the relationship between Helicobacter pylori infection and gastric cancer susceptibility[D].Lanzhou University,2014
[12]张艳梅,马靖华,刘阳晨,等.中国人群mi R-449a单核苷酸多态性与胃癌易感性的相关性研究[J].现代检验医学杂志,2016,31(4):14-18Zhang Yan-mei,Ma Jing-hua,Liu Yang-chen,et al.Study of the correlation between mi R-449a single nucleotide polymorphisms and susceptibility to gastric cancer in Chinese population[J].Journal of modern laboratory medicine,2016,31(4):14-18
[13]李静.Toll样受体1基因多态性与胃癌癌前病变的相关性研究[D].青岛大学,2016Li Jing.Toll receptor like 1 gene polymorphism and gastric precancerous lesions[D].Qiingdao University,2016
[14]钟慧钰,宋兴勃,周娟,等.调控mi RNA成熟通路的相关基因多态性与四川汉族人群胃癌的相关性研究[J].中国输血杂志,2015,28(4):367-370Zhong Hui-yu,Song Xing-bo,Zhou Juan,et al.Study on the association between polymorphisms in mi RNA mature pathway and gastric cancer in Han population in Sichuan province[J].Chinese Journal of transfusion,2015,28(4):367-370
[15]Cai M,Dai S,Chen W,et al.Environmental factors,seven GWAS-identified susceptibility loci,and risk of gastric cancer and its precursors in a Chinese population[J].Cancer Medicine,2017,6(3):708-714
[16]刘淑红.XPG和XRCC1基因多态性与奥沙利铂对晚期胃癌疗效的相关性研究[D].山东大学,2015Liu Shu-hong.XPG and XRCC1 gene polymorphisms and oxaliplatin in the treatment of advanced gastric cancer[D].Shandong University,2015
[17]王建凯,蔡宏懿,缪国英,等.中国人群亚甲基四氢叶酸还原酶C677T基因位点的多态性与胃癌关系的meta分析[J].中国普外基础与临床杂志,2017,38(6):109-112Wang Jian-kai,Cai Hong-yi,Miu Guo-ying,et al.Clinical journal,polymorphisms of methylenetetrahydrofolate reductase C677T gene Chinese in the relationship between gastric cancer and meta analysis[J].Chinese surgery 2017,38(6):109-112
[18]Grubert F,Zaugg J,Kasowski M,et al.Genetic Control of Chromatin States in Humans Involves Local and Distal Chromosomal Interactions[J].Cell,2015,162(5):1051-1057
[19]毛华杰,朱燕,卢淑华,等.硒蛋白S基因G-105A、G-254A位点多态性与湖南汉族人群胃癌遗传易感性的相关研究[J].肿瘤防治研究,2015,42(6):582-586Mao Hua-jie,Zhu Yan,Lu Shu-hua,et al.Selenoprotein S gene G-105A and G-254A polymorphisms and susceptibility to gastric cancer in Hunan Han population related research[J].cancer research,2015,42(6):582-586
[20]沈宏,张宁.亚甲基四氢叶酸还原酶基因C677T和A1298C位点多态性与胃癌易感性的相关性分析[J].中国普外基础与临床杂志,2015,(3):120-124Shen Hong,Zhang Ning.Association of polymorphisms of methylenetetrahydrofolate reductase gene C677T and A1298C with susceptibility to gastric cancer[J].Journal of clinical and basic China surgery,2015,(3):120-124