金针菇蛛网病病原菌鉴定
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摘要
近期在山东省金针菇(Flammulina velutipes)的生产中发现一种蛛网病病害。为明确蛛网病病害的致病菌,通过感病子实体的组织分离,获得了病原菌菌株JN,经过致病性测定、形态特征观察和rDNA-ITS序列分析,确定该病原菌为金黄菌寄生(Hypomyces aurantius)。
Recently, a cobweb disease was found in the production of Flammulina velutipes in Shandong province. In order to identify the pathogen, JN strain was obtained by tissue isolation from the susceptible fruiting body. Pathogenicity test, morphological observation and r DNA-ITS sequence analysis confirmed that the pathogen was Hypomyces aurantius.
Recently, a cobweb disease was found in the production of Flammulina velutipes in Shandong province. In order to identify the pathogen, JN strain was obtained by tissue isolation from the susceptible fruiting body. Pathogenicity test, morphological observation and r DNA-ITS sequence analysis confirmed that the pathogen was Hypomyces aurantius.
引文
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[10]蔡为明,方菊莲,范雷法,等.金针菇软腐病病原菌鉴定及生物学特性的观察[J].浙江农业学报,1993,5(2):99-103.
[11]张琪辉,王威,李成欢,等.斑玉蕈蛛网病的病原菌及其生物学特性[J].菌物学报,2015,34(3):350-356.
[12]Chang GB,Chang YL,Geon SS,et al.Firstoccurrence of cobweb disease on Hypsizigus marmoreus caused by Cladobotryum varium in Korea[J].Journal of the Faculty of Agriculture Kyushu University,2012,57(2):373-377.
[13]Lan YF,Wang QW,Yu CX,et al.First report of Cladobotryum mycophilum causing cobweb disease on Agaricus bisporus in China[J].Plant Disease,2016,100(11):2334.
[14]兰玉菲,安秀荣,王庆武,等.双孢蘑菇蛛网病病菌生物学特性研究[J].中国食用菌,2017,36(4):62-65.
[15]兰玉菲,唐丽娜,李秀梅.双孢蘑菇蛛网病的发生特点与防控技术[J].食用菌,2018(1):66-67.
[2]Cai H,Liu X,Chen Z,et al.Isolation,purification and identification of nine chemical compounds from Flammulina velutipes fruiting bodies[J].Food Chemistry,2013,141(3):2873-2879.
[3]方中达.植病研究方法[M].3版.北京:中国农业出版社,1998:122-145.
[4]Chang GB,Chang YL,Geon SS,et al.Characterization of species of Cladobotryum which cause cobweb disease in edible mushrooms grown in Korea[J].Mycobiology,2012,40(3):189-194.
[5]兰玉菲,蔚承祥,王庆武,等.一种山山东地栽香菇病害病原菌的分离及鉴定[J].中国食用菌,2015,34(6):74-76.
[6]White TJ,Bruns T,Lee S,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[C]//Innis MA,Gelfand DH,Sninsky JJ.(eds.).PCR protocols:a guide to methods and applications.New York:Academic Press,1990:315-322.
[7]Kirk PM,Cannon PF,Minter DW,et al.Ainsworth&Bisby’s dictionary of the fungi[M].10th edition.Wallingford:CAB International Publishing,2008:1-771.
[8]曾昭清,庄文颖.菌寄生属研究进展[J].菌物学报,2015,34(5):809-816.
[9]Teng SC.Notes on Hypocreales from China[J].Sinensia,1934(4):269-298.
[10]蔡为明,方菊莲,范雷法,等.金针菇软腐病病原菌鉴定及生物学特性的观察[J].浙江农业学报,1993,5(2):99-103.
[11]张琪辉,王威,李成欢,等.斑玉蕈蛛网病的病原菌及其生物学特性[J].菌物学报,2015,34(3):350-356.
[12]Chang GB,Chang YL,Geon SS,et al.Firstoccurrence of cobweb disease on Hypsizigus marmoreus caused by Cladobotryum varium in Korea[J].Journal of the Faculty of Agriculture Kyushu University,2012,57(2):373-377.
[13]Lan YF,Wang QW,Yu CX,et al.First report of Cladobotryum mycophilum causing cobweb disease on Agaricus bisporus in China[J].Plant Disease,2016,100(11):2334.
[14]兰玉菲,安秀荣,王庆武,等.双孢蘑菇蛛网病病菌生物学特性研究[J].中国食用菌,2017,36(4):62-65.
[15]兰玉菲,唐丽娜,李秀梅.双孢蘑菇蛛网病的发生特点与防控技术[J].食用菌,2018(1):66-67.