2种单体皂苷及三七总皂苷对白菜幼苗生长生理的影响
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摘要
研究了三七皂苷R_1、人参皂苷Rg1和三七总皂苷对白菜幼苗苗高、主根长等生长性状以及过氧化物酶(POD)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活性,可溶性糖、可溶性蛋白质含量等生理特性的影响。结果表明,与对照(蒸馏水)相比,0. 01～100. 00 mg/L三七皂苷R_1和人参皂苷Rg1处理均不同程度地降低了白菜幼苗的苗高、主根长和POD、SOD活性,其中,10. 00、100. 00mg/L三七皂苷R_1处理组和0. 01、50. 00 mg/L人参皂苷Rg1处理组白菜幼苗的主根长降低达显著水平,白菜幼苗CAT活性升高,可溶性糖含量和可溶性蛋白质含量增加;三七总皂苷处理能增加白菜幼苗的SOD、CAT活性和可溶性蛋白质含量,降低其主根长、POD活性,而苗高、可溶性糖含量呈现低促高抑的趋势,其中,0. 01、10. 00、50. 00、100. 00 mg/L处理组白菜幼苗POD活性分别比对照显著下降35. 57%、37. 90%、45. 35%、35. 42%。三七皂苷R_1、人参皂苷Rg1及三七总皂苷对白菜幼苗均具有一定的化感抑制作用,但在质量浓度0. 01～100. 00 mg/L的范围内,白菜可以作为三七的轮作作物。
This article studied the effects of notoginsenoside R_1,ginsenoside Rg_1 and PNS on the growth features of Chinese cabbage seedlings such as height of seedings,main root length,as well as physiological characteristics such as POD,SOD,CAT activity,soluble sugar contents,soluble protein contents,etc. The research results indicated that,compared with control group,the treatment of notoginsenoside R_1 and ginsenoside Rg_1 with the concentration range from 0. 01 to 100. 00 mg/L,reduced the height,main root length,POD and SOD activity of Chinese cabbage seedlings in varying degrees. The treatments of 10. 00,100. 00 mg/L notoginsenoside R_1 and 0. 01,50. 00 mg/L ginsenoside Rg_1,significantly reduced the main root length,increased CAT activity,soluble sugar content and soluble protein content of Chinese cabbage seedlings. The PNS treatment increased SOD,CAT activity and soluble protein content of cabbage seed-lings,and reduced main root length and POD activity of Chinese cabbage seedlings. Howerver,when PNS treatment with different concentration was carried out,the height and soluble sugar content of Chinese cabbage seedlings showed the trend of low concentrations promotion and high concentrations inhibition.Compared with control group,the POD activity with the PNS treatment of 0. 01,10. 00,50. 00,100. 00mg/L decreased by 35. 57%,37. 90%,45. 35%,35. 42%,respectively. Notoginsenoside R_1,ginsenoside Rg_1 and PNS have certain inhibitory effects on Chinese cabbage seedlings,but in the concentration range of 0. 01—100. 00 mg/L,Chinese cabbage can be used as a rotation crop of Panax notoginseng.
This article studied the effects of notoginsenoside R_1,ginsenoside Rg_1 and PNS on the growth features of Chinese cabbage seedlings such as height of seedings,main root length,as well as physiological characteristics such as POD,SOD,CAT activity,soluble sugar contents,soluble protein contents,etc. The research results indicated that,compared with control group,the treatment of notoginsenoside R_1 and ginsenoside Rg_1 with the concentration range from 0. 01 to 100. 00 mg/L,reduced the height,main root length,POD and SOD activity of Chinese cabbage seedlings in varying degrees. The treatments of 10. 00,100. 00 mg/L notoginsenoside R_1 and 0. 01,50. 00 mg/L ginsenoside Rg_1,significantly reduced the main root length,increased CAT activity,soluble sugar content and soluble protein content of Chinese cabbage seedlings. The PNS treatment increased SOD,CAT activity and soluble protein content of cabbage seed-lings,and reduced main root length and POD activity of Chinese cabbage seedlings. Howerver,when PNS treatment with different concentration was carried out,the height and soluble sugar content of Chinese cabbage seedlings showed the trend of low concentrations promotion and high concentrations inhibition.Compared with control group,the POD activity with the PNS treatment of 0. 01,10. 00,50. 00,100. 00mg/L decreased by 35. 57%,37. 90%,45. 35%,35. 42%,respectively. Notoginsenoside R_1,ginsenoside Rg_1 and PNS have certain inhibitory effects on Chinese cabbage seedlings,but in the concentration range of 0. 01—100. 00 mg/L,Chinese cabbage can be used as a rotation crop of Panax notoginseng.
引文
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[26]张秋菊,张爱华,杨鹤,等.人参皂苷对4种栽培作物早期根系发育的化感效应[J].植物分类与资源学报,2014,39(4):594-596.
[27]玄晓丽,陈梦怡,马三梅. ABA对叶子花正常叶和变态叶部分生理生化指标的影响[J].广西植物,2012,32(6):806-809.
[28]詹嘉红,蓝宗辉.水淹对铺地黍部分生理指标的影响[J].广西植物,2011,31(6):823-826.
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[2]黄天卫,张文斌,孙玉琴,等.不同轮作年限三七总皂苷含量分析[J].现代中药研究与实践,2011,25(3):3-4.
[3]张重义,李明杰,陈新建,等.地黄连作障碍机制的研究进展与消减策略[J].中国现代中药,2013,15(1):38-44.
[4]张子龙,李凯明,杨建忠,等.轮作对三七连作障碍的消减效应研究[J].西南大学学报(自然科学版),2015,37(8):39-46.
[5]游佩进,王文全,张媛,等.三七根区土壤提取物对三七幼苗的化感作用[J].西南农业学报,2009,22(2):308-310.
[6]游佩进,王文全,张媛,等.三七连作土壤对三七、莴苣的化感作用[J].西北农业学报,2009,18(1):139-142.
[7]张子龙,王文全,杨建忠,等.三七连作土壤对其种子萌发及幼苗生长的影响[J].土壤,2010,42(6):1009-1013.
[8]拱健婷,程新宇,孙萌,等. 3种皂苷单体对三七种子萌发及幼苗生长的影响[J].江西农业大学学报,2015,37(6):988-993.
[9]游佩进,张媛,王文全,等.三七连作土壤对几种蔬菜种子及幼苗的化感作用[J].中国现代中药,2009,11(5):12-13.
[10]张金燕,孙雪婷,陈军文,等.连作三七根际土壤化感物质检测及其提取液对三种作物种子萌发的影响[J].南方农业学报,2017,48(7):1178-1184.
[11]王田涛,卢丙越,雷恩,等.三七对不同作物化感的效应[J].浙江农业科学,2017,58(11):1982-1985,1988.
[12]王庆玲,董涛,张子龙.三七对小麦的化感作用[J].生态学杂志,2015,34(2):431-437.
[13]杨莉,朱艳,崔秀明,等.三七植株残体降解物对小麦根系活力及生理生化指标的影响[J].西南农业学报,2015,28(5):1961-1964.
[14]王雄飞,刘春生,高鹏,等.三七水提液对几种植物种子萌发和幼苗生长的化感作用[J].中国农学通报,2014,30(4):299-303.
[15]沈玉聪,张红瑞,张子龙,等.酚酸类物质对三七幼苗的化感影响[J].广西植物,2016,36(5):607-614.
[16]李贺敏,张红瑞,沈玉聪,等.酚酸类物质对白菜幼苗和生理特性的影响[J].河南农业大学学报,2017,51(5):626-633.
[17]沈玉聪,张红瑞,姚珊,等. 5种酚酸类物质对小麦幼苗的化感作用研究[J].河南农业科学,2016,45(5):101-105.
[18]沈玉聪.酚酸和皂苷类物质对三七及3种主要轮作作物幼苗的化感作用研究[D].郑州:河南农业大学,2016.
[19]黄勇,张红瑞,沈玉聪,等.酚酸类物质对玉米幼苗生长及生理特性的影响[J].河南农业大学学报,2017,51(3):301-307.
[20]周家明,张文斌,杨建忠,等.三七根际土壤化感物质的初步分离鉴定[J].现代中药研究与实践,2012,26(1):14-16.
[21]韦美丽,孙玉琴,黄天卫,等.化感物质对三七生长的影响[J].特产研究,2010(1):32-34.
[22]周艳,张红瑞,沈玉聪,等.三七总皂苷及两种单体皂苷对三七幼苗的化感作用研究[J].河南农业大学学报,2017,51(6):786-791.
[23]李玲.植物生理学模块实验指导[M].北京:科学出版社,2009:97-98.
[24]孔祥生,易现峰.植物生理学实验技术[M].北京:中国农业出版社,2008.
[25]张子龙,侯俊玲,王文全,等.三七水浸液对不同玉米品种的化感作用[J].中国中药杂志,2014,39(4):594-596.
[26]张秋菊,张爱华,杨鹤,等.人参皂苷对4种栽培作物早期根系发育的化感效应[J].植物分类与资源学报,2014,39(4):594-596.
[27]玄晓丽,陈梦怡,马三梅. ABA对叶子花正常叶和变态叶部分生理生化指标的影响[J].广西植物,2012,32(6):806-809.
[28]詹嘉红,蓝宗辉.水淹对铺地黍部分生理指标的影响[J].广西植物,2011,31(6):823-826.
[29]王松华,杨志敏,吕波,等.印度芥菜对Cu诱导的氧化胁迫响应[J].南京农业大学学报,2004,27(1):24-27.