二倍体马铃薯S.phureja种质资源的抗旱性鉴定与综合评价
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摘要
本研究采用盆栽干旱法,对19个二倍体马铃薯S. phureja种质资源和1个四倍体品种进行抗旱性鉴定,测定了叶片含水量、相对电导率、叶绿素含量、丙二醛含量、可溶性糖含量、脯氨酸含量等生理生化指标,利用主成分分析和隶属函数分析综合评价其抗旱性。试验结果表明,品种间干旱胁迫响应差异显著,干旱处理下生理生化指标与对照相比差异显著,通过主成分分析和隶属函数分析,筛选出C3、C4、C6、C9、C10这5个二倍体马铃薯抗旱种质。本研究通过多个指标鉴定马铃薯抗旱性可为抗旱育种提供技术参考,鉴定出的S. phureja抗旱种质可为二倍体马铃薯抗旱育种提供材料基础。
In this study, identification of drought resistance in 19 diploid potato S. phureja germplasm resources and 1 tetraploid variety were performed by pot drought method. The physiological and biochemical indexes of leaf water content, relative conductivity, chlorophyll content, malondialdehyde content, soluble sugar content and proline content were measured, and the drought resistance was comprehensively evaluated by principal component analysis and membership function analysis. The test results showed that the responses of drought stress were significantly different among varieties, and the physiological and biochemical indexes under drought treatment were significantly different from those of control. By principal component analysis and membership function analysis, C3, C4, C6, C9 and C10 these five drought-resistant germplasm were screened out. In this study, drought resistance of potato was identified by multiple indexes, which could provide technical reference for drought resistance breeding. Drought resistance germplasm S. phureja could provide material basis for drought resistance breeding of diploid potato.
In this study, identification of drought resistance in 19 diploid potato S. phureja germplasm resources and 1 tetraploid variety were performed by pot drought method. The physiological and biochemical indexes of leaf water content, relative conductivity, chlorophyll content, malondialdehyde content, soluble sugar content and proline content were measured, and the drought resistance was comprehensively evaluated by principal component analysis and membership function analysis. The test results showed that the responses of drought stress were significantly different among varieties, and the physiological and biochemical indexes under drought treatment were significantly different from those of control. By principal component analysis and membership function analysis, C3, C4, C6, C9 and C10 these five drought-resistant germplasm were screened out. In this study, drought resistance of potato was identified by multiple indexes, which could provide technical reference for drought resistance breeding. Drought resistance germplasm S. phureja could provide material basis for drought resistance breeding of diploid potato.
引文
Bai J.P.,Gao H.J.,Yang H.Y.,Lou Y.,Zhang J.L.,Wang D.,and Zhang J.L.,2016,Comparison of ultrastructural and physio logical changes of potato(Solanum tuberosum L.)plantlets subjected to salt and modeling drought stresses,Acta Physiol.Plant,38(7):1-9
Cabello R.,De Mendiburu F.,Bonierbale M.,Monneveux P.,Roca W.,and Chujoy E.,2012,Large-scale evaluation of potato improved varieties,genetic stocks and landraces for drought tolerance,Am.J.Potato Res.,89(5):400-410
Cabello R.,Monneveux P.,De Mendiburu F.,and Bonierbale M.,2013,Comparison of yield based drought tolerance indices in improved varieties,genetic stocks and landraces of potato(Solanum tuberosum L.),Euphytica,193(2):147-156
Chao X.J.,Kong W.G.,Zhang Y.,Liu S.X.,Yang Y.,and Li G.C.,2008,Diploid potato germplasm resources and their application,Shandong Nongye Kexue(Shandong Agricultural Sciences),(7):49-53(晁祥健,孔维国,张煜,刘生祥,杨煜,李广存,2008,二倍体马铃薯资源现状及应用研究进展,山东农业科学,(7):49-53)
De Jong H.,2016,Impact of the potato on society,Am.J.Potato Res.,93(5):415-429
Deng Z.,Xu J.F.,Duan S.G.,Liu J.,Bian C.S.,Pang W.F.,and Jin L.P.,2014,Effect on growth indicators of 11 potato cultivars under PEG-8000 stress,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),29(5):99-106(邓珍,徐建飞,段绍光,刘杰,卞春松,庞万福,金黎平,2014,PEG-8000模拟干旱胁迫对11个马铃薯品种的组培苗生长指标的影响,华北农学报,29(5):99-106)
Ding Y.M.,Ma L.H.,Zhou X.G.,Yao C.X.,Dong L.F.,and Sun M.L.,2013,Effects of drought stress on free proline and malonaldedyde contents in potato leaves and correlation analysis of drought-tolerant level among different varieties,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),26(1):106-110(丁玉梅,马龙海,周晓罡,姚春馨,董禄风,孙茂林,2013,干旱胁迫下马铃薯叶片脯氨酸、丙二醛含量变化及与耐旱性的相关性分析,西南农业学报,26(1):106-110)
Eiasu B.K.,Soundy P.,and Hammes P.S.,2007,Response of potato(Solarium tuberosum)tuber yield components to gelpolymer soil amendments and irrigation regimes,New Zeal.J.Crop Hort.,35(1):25-31
Ghislain M.,Andrade D.,Rodríguez F.,Hijmans R.J.,and Spooner D.M.,2006,Genetic analysis of the cultivated potato Solanum tuberosum L.Phureja group using RAPDs and nuclear SSRs,Theor.Appl.Genet.,113(8):1515-1527
Gong L.,Zhang L.,Nie F.J.,Chen Y.C.,Gan X.Y.,Shi L.,Song Y.X.,Guo Z.Q.,and Wang F.,2015,Transcriptome analysis on transcription factors of potato(Solanum tuberosum)under drought stress and rehydration treatment,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(8):1745-1756(巩檑,张丽,聂峰杰,陈虞超,甘晓燕,石磊,宋玉霞,郭志乾,王峰,2015,旱胁迫和复水处理后马铃薯转录因子的转录组分析,分子植物育种,13(8):1745-1756)
Gong M.,1989,Screening methods and indexes of drought resistance in crops and comprehensive evaluation,Yunnan Nongye Daxue Xuebao(Journal of Yunnan Agricultural U-niversity),4(1):73-81(龚明,1989,作物抗旱性鉴定方法与指标及其综合评价,云南农业大学学报,4(1):73-81)
Hai M.R.,Chen Y.,Zhou P.,Wang L.,Liu F.C.,Tang T.X.,and Dabu X.L.T.,2014,Effects of drought stress on physiological characteristics of potato(Solanum tuberosum L.),Zhongguo Malingshu(Chinese Potato Journal),(4):199-204(海梅荣,陈勇,周平,王丽,刘福翠,唐天向,达布希拉图,2014,干旱胁迫对马铃薯品种生理特性的影响,中国马铃薯,(4):199-204)
Hui D.J.,Ji Y.M.,Li R.M.,and Guo J.C.,2011,Preliminary study on method for determination of chlorophyll in cassava leaves,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),30(43):1279-1283(惠杜娟,冀乙萌,李瑞梅,郭建春,2011,木薯中两种叶绿素提取方法的比较及其含量测定,基因组学与应用生物学,30(43):1279-1283)
Jia H.,Muge Q.,Rigen L.,He X.Q.,and Meng M.L.,2016,Sodium nitroprusside role on antioxidant enzymes activities in leaves of different potato cultivars under drought stress,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),36(3):551-557(贾慧,其力木格,李特日根,贺学勤,蒙美莲,2016,外源SNP对干旱胁迫下不同马铃薯品种叶片抗氧化酶活性的影响,西北植物学报,36(3):551-557)
Li Y.,Li G.C.,Li C.H.,Qu D.Y.,and Huang S.W.,2013,Prospects of diploid hybrid breeding in potato,Zhongguo Malingshu(Chinese Potato Journal),(2):96-99(李颖,李广存,李灿辉,屈冬玉,黄三文,2013,二倍体杂种优势马铃薯育种的展望,中国马铃薯,(2):96-99)
Li Y.,Lyu D.Q.,Hu L.S.,Li X.Z.,Gu H.B.,Qu L.,Xu F.F.,Wang S.P.,Liu S.W.,Liu Z.Y.,Chen J.X.,Li H.J.,Yang H.C.,and Du Y.Q.,2013,Study on genetic performance of defective of potato seed offspring,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(8):9-11(李勇,吕典秋,胡林双,李学湛,顾海滨,曲丽,宿飞飞,王绍鹏,刘尚武,刘振宇,陈江先,李洪军,杨焕春,杜英秋,2013,马铃薯外观缺陷种薯后代薯形的遗传表现研究,黑龙江农业科学,(8):9-11)
Li Y.J.,2013,Study on the hydraulic lift ability and the drought tolerance of potato,Thesis for M.S.,Gansu Agricultural University,Supervisor:Wang D.,and Bai J.P.,pp.4-16(李亚杰,2013,不同马铃薯品种根系提水能力与抗旱性研究,硕士学位论文,甘肃农业大学,导师:王蒂,白江平,pp.4-16)
Mackerron D.K.L.,and Jefferies R.A.,1988,The distributions of tuber sizes in droughted and irrigated crops of potato.I.Observations on the effect of water stress on graded yields from differing cultivars,Potato Research,31(2):269-278
Monneveux P.,Ramírez D.A.,and Pino M.T.,2013,Drought tolerance in potato(S.tuberosum L.):Can we learn from drought tolerance research in cereals,Plant Science,205-206(9):76-86
Schafleitner R.,Gutierrez R.,Espino R.,Gaudin A.,Pérez J.,Martínez M.,Domínguez A.,Tincopa L.,Alvarado C.,Numberto G.,and Bonierbale M.,2007,Field screening for variation of drought tolerance in Solanum tuberosum L.by agronomical,physiological and genetic analysis,Potato Research,50(1):71-85
Xie W.,and Zheng W.L.,2015,The evaluation indexes and methods on drought resistance of potato,Xizang Nongye Keji(Tibet Journal of Agricultural Sciences),37(4):27-35(谢婉,郑维列,2015,马铃薯抗旱评价指标及方法,西藏农业科技,37(4):27-35)
Yang H.H.,and Guo H.C.,2017,Comprehensive evalution of cold resistence of potato varieties,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(2):716-724(杨慧菊,郭华春,2017,马铃薯不同品种抗寒性综合评价,分子植物育种,15(2):716-724)
Yang H.Y.,Ping H.T.,Wang D.,Wang L.,Liu Y.H.,Bai J.P.,Yu B.,and Zhang J.L.,2016,Drought resistance of different ploidy potato varieties,Zhongguo Shamo(Journal of Desert Research),36(4):1041-1049(杨宏羽,平海涛,王蒂,王丽,刘玉汇,白江平,余斌,张俊莲,2016,不同倍性马铃薯品种的抗旱性,中国沙漠,36(4):1041-1049)
Cabello R.,De Mendiburu F.,Bonierbale M.,Monneveux P.,Roca W.,and Chujoy E.,2012,Large-scale evaluation of potato improved varieties,genetic stocks and landraces for drought tolerance,Am.J.Potato Res.,89(5):400-410
Cabello R.,Monneveux P.,De Mendiburu F.,and Bonierbale M.,2013,Comparison of yield based drought tolerance indices in improved varieties,genetic stocks and landraces of potato(Solanum tuberosum L.),Euphytica,193(2):147-156
Chao X.J.,Kong W.G.,Zhang Y.,Liu S.X.,Yang Y.,and Li G.C.,2008,Diploid potato germplasm resources and their application,Shandong Nongye Kexue(Shandong Agricultural Sciences),(7):49-53(晁祥健,孔维国,张煜,刘生祥,杨煜,李广存,2008,二倍体马铃薯资源现状及应用研究进展,山东农业科学,(7):49-53)
De Jong H.,2016,Impact of the potato on society,Am.J.Potato Res.,93(5):415-429
Deng Z.,Xu J.F.,Duan S.G.,Liu J.,Bian C.S.,Pang W.F.,and Jin L.P.,2014,Effect on growth indicators of 11 potato cultivars under PEG-8000 stress,Huabei Nongxuebao(Acta Agriculturae Boreali-Sinica),29(5):99-106(邓珍,徐建飞,段绍光,刘杰,卞春松,庞万福,金黎平,2014,PEG-8000模拟干旱胁迫对11个马铃薯品种的组培苗生长指标的影响,华北农学报,29(5):99-106)
Ding Y.M.,Ma L.H.,Zhou X.G.,Yao C.X.,Dong L.F.,and Sun M.L.,2013,Effects of drought stress on free proline and malonaldedyde contents in potato leaves and correlation analysis of drought-tolerant level among different varieties,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),26(1):106-110(丁玉梅,马龙海,周晓罡,姚春馨,董禄风,孙茂林,2013,干旱胁迫下马铃薯叶片脯氨酸、丙二醛含量变化及与耐旱性的相关性分析,西南农业学报,26(1):106-110)
Eiasu B.K.,Soundy P.,and Hammes P.S.,2007,Response of potato(Solarium tuberosum)tuber yield components to gelpolymer soil amendments and irrigation regimes,New Zeal.J.Crop Hort.,35(1):25-31
Ghislain M.,Andrade D.,Rodríguez F.,Hijmans R.J.,and Spooner D.M.,2006,Genetic analysis of the cultivated potato Solanum tuberosum L.Phureja group using RAPDs and nuclear SSRs,Theor.Appl.Genet.,113(8):1515-1527
Gong L.,Zhang L.,Nie F.J.,Chen Y.C.,Gan X.Y.,Shi L.,Song Y.X.,Guo Z.Q.,and Wang F.,2015,Transcriptome analysis on transcription factors of potato(Solanum tuberosum)under drought stress and rehydration treatment,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(8):1745-1756(巩檑,张丽,聂峰杰,陈虞超,甘晓燕,石磊,宋玉霞,郭志乾,王峰,2015,旱胁迫和复水处理后马铃薯转录因子的转录组分析,分子植物育种,13(8):1745-1756)
Gong M.,1989,Screening methods and indexes of drought resistance in crops and comprehensive evaluation,Yunnan Nongye Daxue Xuebao(Journal of Yunnan Agricultural U-niversity),4(1):73-81(龚明,1989,作物抗旱性鉴定方法与指标及其综合评价,云南农业大学学报,4(1):73-81)
Hai M.R.,Chen Y.,Zhou P.,Wang L.,Liu F.C.,Tang T.X.,and Dabu X.L.T.,2014,Effects of drought stress on physiological characteristics of potato(Solanum tuberosum L.),Zhongguo Malingshu(Chinese Potato Journal),(4):199-204(海梅荣,陈勇,周平,王丽,刘福翠,唐天向,达布希拉图,2014,干旱胁迫对马铃薯品种生理特性的影响,中国马铃薯,(4):199-204)
Hui D.J.,Ji Y.M.,Li R.M.,and Guo J.C.,2011,Preliminary study on method for determination of chlorophyll in cassava leaves,Jiyinzuxue Yu Yingyong Shengwuxue(Genomics and Applied Biology),30(43):1279-1283(惠杜娟,冀乙萌,李瑞梅,郭建春,2011,木薯中两种叶绿素提取方法的比较及其含量测定,基因组学与应用生物学,30(43):1279-1283)
Jia H.,Muge Q.,Rigen L.,He X.Q.,and Meng M.L.,2016,Sodium nitroprusside role on antioxidant enzymes activities in leaves of different potato cultivars under drought stress,Xibei Zhiwu Xuebao(Acta Botanica Boreali-Occidentalia Sinica),36(3):551-557(贾慧,其力木格,李特日根,贺学勤,蒙美莲,2016,外源SNP对干旱胁迫下不同马铃薯品种叶片抗氧化酶活性的影响,西北植物学报,36(3):551-557)
Li Y.,Li G.C.,Li C.H.,Qu D.Y.,and Huang S.W.,2013,Prospects of diploid hybrid breeding in potato,Zhongguo Malingshu(Chinese Potato Journal),(2):96-99(李颖,李广存,李灿辉,屈冬玉,黄三文,2013,二倍体杂种优势马铃薯育种的展望,中国马铃薯,(2):96-99)
Li Y.,Lyu D.Q.,Hu L.S.,Li X.Z.,Gu H.B.,Qu L.,Xu F.F.,Wang S.P.,Liu S.W.,Liu Z.Y.,Chen J.X.,Li H.J.,Yang H.C.,and Du Y.Q.,2013,Study on genetic performance of defective of potato seed offspring,Heilongjiang Nongye Kexue(Heilongjiang Agricultural Sciences),(8):9-11(李勇,吕典秋,胡林双,李学湛,顾海滨,曲丽,宿飞飞,王绍鹏,刘尚武,刘振宇,陈江先,李洪军,杨焕春,杜英秋,2013,马铃薯外观缺陷种薯后代薯形的遗传表现研究,黑龙江农业科学,(8):9-11)
Li Y.J.,2013,Study on the hydraulic lift ability and the drought tolerance of potato,Thesis for M.S.,Gansu Agricultural University,Supervisor:Wang D.,and Bai J.P.,pp.4-16(李亚杰,2013,不同马铃薯品种根系提水能力与抗旱性研究,硕士学位论文,甘肃农业大学,导师:王蒂,白江平,pp.4-16)
Mackerron D.K.L.,and Jefferies R.A.,1988,The distributions of tuber sizes in droughted and irrigated crops of potato.I.Observations on the effect of water stress on graded yields from differing cultivars,Potato Research,31(2):269-278
Monneveux P.,Ramírez D.A.,and Pino M.T.,2013,Drought tolerance in potato(S.tuberosum L.):Can we learn from drought tolerance research in cereals,Plant Science,205-206(9):76-86
Schafleitner R.,Gutierrez R.,Espino R.,Gaudin A.,Pérez J.,Martínez M.,Domínguez A.,Tincopa L.,Alvarado C.,Numberto G.,and Bonierbale M.,2007,Field screening for variation of drought tolerance in Solanum tuberosum L.by agronomical,physiological and genetic analysis,Potato Research,50(1):71-85
Xie W.,and Zheng W.L.,2015,The evaluation indexes and methods on drought resistance of potato,Xizang Nongye Keji(Tibet Journal of Agricultural Sciences),37(4):27-35(谢婉,郑维列,2015,马铃薯抗旱评价指标及方法,西藏农业科技,37(4):27-35)
Yang H.H.,and Guo H.C.,2017,Comprehensive evalution of cold resistence of potato varieties,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),15(2):716-724(杨慧菊,郭华春,2017,马铃薯不同品种抗寒性综合评价,分子植物育种,15(2):716-724)
Yang H.Y.,Ping H.T.,Wang D.,Wang L.,Liu Y.H.,Bai J.P.,Yu B.,and Zhang J.L.,2016,Drought resistance of different ploidy potato varieties,Zhongguo Shamo(Journal of Desert Research),36(4):1041-1049(杨宏羽,平海涛,王蒂,王丽,刘玉汇,白江平,余斌,张俊莲,2016,不同倍性马铃薯品种的抗旱性,中国沙漠,36(4):1041-1049)