IL-10在H.pylori相关性胃炎组织中的表达及与COX-2、iNOS的相关性
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摘要
目的:观察IL-10在H. pylori相关性胃炎组织中的表达及与COX-2、iNOS的相关性。方法:选取74例H. pylori相关性胃炎患者,均行H. pylori检测确定H. pylori感染情况,行病理组织学检测明确病理分型、胃黏膜炎症程度,行免疫组化检测胃黏膜中IL-10、COX-2、iNOS表达。分析H. pylori感染情况、病理诊断与组织分型结果,明确慢性胃炎患者H. pylori感染与病理炎症程度、病理分型的关系、IL-10表达与H. pylori感染的关系、比较H. pylori阳性胃炎不同病理类型中COX-2、iNOS表达情况,分析H. pylori阳性胃炎患者IL-10与COX-2、iNOS表达的相关性。结果:74例慢性胃炎患者中,H. pylori阳性59例,阴性15例,H. pylori检出率79. 73%。H. pylori阳性与阴性患者在炎症程度的分布方面有显著差异(P <0. 05),表现为H. pylori阳性患者中重度炎症患者居多,而阴性患者则以轻度为主。74例患者中31例为IL-10阳性、43例为IL-10阴性。IL-10阳性患者中的H. pylori阳性率(93. 55%)显著高于IL-10阴性(69. 77%)患者(P <0. 05);病理分型的差异无统计学意义(P> 0. 05)。COX-2阳性表达率在H. pylori阳性患者中为59. 32%,显著高于H. pylori阴性患者的20. 00%(P <0. 01); iNOS阳性表达率为61. 02%,显著高于阴性患者的2 0. 0 0%(P<0. 01)。H. pylori阳性不同病理类型胃炎患者的IL-10阳性率差异无统计学意义(P> 0. 05); H. pylori阳性胃炎(IM+Dy)患者的COX-2阳性率(84. 62%)高于CSG(44. 00%)患者(P <0. 05); CAG患者的iNOS阳性率(80. 95%)显著高于CSG(44. 00%)患者(P <0. 05)。H. pylori阳性胃炎患者的IL-10表达与COX-2(r=-0. 566,P <0. 001)、iNOS(r=-0. 465,P <0. 001)表达均呈现显著负相关。结论:IL-10为H. pylori相关性胃炎的保护因子,在H. pylori阳性患者中具有高阳性率。且IL-10与H. pylori阳性患者的COX-2、iNOS表达呈较强负相关,可通过对COX-2、iNOS表达的下调作用降低慢性胃炎患者的恶性病变风险。
Objective: To observe the expression of IL-10 in H. pylori-associated gastritis and its correlation with COX-2 and iNOS. Methods: 74 cases of H. pylori related gastritis were selected. H. pylori was tested to determine H. pylori infection. The histopathological examination was tested to determine the pathological type,the degree of inflammation of the gastric mucosa. The immunohistochemistry was used to detect the expression of IL-10,COX-2 and iNOS in gastric mucosa. The H. pylori infection,pathological diagnosis and histological typing results were analyzed. The relationships between H. pylori infection and pathologic inflammation and pathological type in patients with chronic gastritis were analyzed. The relationship between IL-10 expression and H. pylori infection was analyzed. The expression of COX-2 and iNOS in H. pylori-positive gastritis in different pathological types were compared. The correlations between IL-10 and COX-2,iNOS expression in H. pylori positive gastritis patients were analyzed. Results: In 74 cases of chronic gastritis patients,59 cases were H. pylori positive and 15 cases were negative,the H. pylori detection rate was 79. 73%. The patients of H. pylori positive and negative was significantly different in the degree of inflammation of the distribution( P < 0. 05),manifested as H. pylori positive patients with moderate to severe inflammation,while the negative patients were mild mainly. The positive rate of H. pylori( 93. 55%) in IL-10 positive patients was significantly higher than that in patients with IL-10 negative( 69. 77%)( P < 0. 05). There was no significant difference in pathological type( P > 0. 05). The positive expression rate of COX-2 in H. pylori positive patients was 59. 32%,which was significantly higher than that in H. pylori negative patients( 20. 00%)( P < 0. 01),and the positive expression rate of iNOS was 61. 02%,which was significantly higher than that of negative patients( 20. 00%)( P < 0. 01). There was no significant difference in the positive rate of IL-10 between different pathologic gastritis H. pylori positive patients( P > 0. 05). The positive rate of COX-2( 84. 62%) in patients with H. pylori positive gastritis( IM + Dy) was higher than that in CSG( 44. 00%) patients( P < 0. 05),and the positive rate of iNOS( 80. 95%) in CAG patients was significantly higher than that in patients with CSG( 44. 00%)( P < 0. 05). The expression of IL-10 in patients with H. pylori positive gastritis was significantly negatively correlated with COX-2( r =-0. 566,P < 0. 001) and iNOS( r =-0. 465,P < 0. 001). Conclusion: IL-10 is a protective factor for H. pylori-associated gastritis and has a high positive rate in H. pylori-positive patients. The expression of COX-2 and iNOS was negatively correlated with IL-10 in H. pylori positive patients,and could reduce the risk of malignant lesions in patients with chronic gastritis by down-regulating the expression of COX-2 and iNOS.
Objective: To observe the expression of IL-10 in H. pylori-associated gastritis and its correlation with COX-2 and iNOS. Methods: 74 cases of H. pylori related gastritis were selected. H. pylori was tested to determine H. pylori infection. The histopathological examination was tested to determine the pathological type,the degree of inflammation of the gastric mucosa. The immunohistochemistry was used to detect the expression of IL-10,COX-2 and iNOS in gastric mucosa. The H. pylori infection,pathological diagnosis and histological typing results were analyzed. The relationships between H. pylori infection and pathologic inflammation and pathological type in patients with chronic gastritis were analyzed. The relationship between IL-10 expression and H. pylori infection was analyzed. The expression of COX-2 and iNOS in H. pylori-positive gastritis in different pathological types were compared. The correlations between IL-10 and COX-2,iNOS expression in H. pylori positive gastritis patients were analyzed. Results: In 74 cases of chronic gastritis patients,59 cases were H. pylori positive and 15 cases were negative,the H. pylori detection rate was 79. 73%. The patients of H. pylori positive and negative was significantly different in the degree of inflammation of the distribution( P < 0. 05),manifested as H. pylori positive patients with moderate to severe inflammation,while the negative patients were mild mainly. The positive rate of H. pylori( 93. 55%) in IL-10 positive patients was significantly higher than that in patients with IL-10 negative( 69. 77%)( P < 0. 05). There was no significant difference in pathological type( P > 0. 05). The positive expression rate of COX-2 in H. pylori positive patients was 59. 32%,which was significantly higher than that in H. pylori negative patients( 20. 00%)( P < 0. 01),and the positive expression rate of iNOS was 61. 02%,which was significantly higher than that of negative patients( 20. 00%)( P < 0. 01). There was no significant difference in the positive rate of IL-10 between different pathologic gastritis H. pylori positive patients( P > 0. 05). The positive rate of COX-2( 84. 62%) in patients with H. pylori positive gastritis( IM + Dy) was higher than that in CSG( 44. 00%) patients( P < 0. 05),and the positive rate of iNOS( 80. 95%) in CAG patients was significantly higher than that in patients with CSG( 44. 00%)( P < 0. 05). The expression of IL-10 in patients with H. pylori positive gastritis was significantly negatively correlated with COX-2( r =-0. 566,P < 0. 001) and iNOS( r =-0. 465,P < 0. 001). Conclusion: IL-10 is a protective factor for H. pylori-associated gastritis and has a high positive rate in H. pylori-positive patients. The expression of COX-2 and iNOS was negatively correlated with IL-10 in H. pylori positive patients,and could reduce the risk of malignant lesions in patients with chronic gastritis by down-regulating the expression of COX-2 and iNOS.
引文
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[9]Eun Bae S,Hoon Lee J,Soo Park Y,et al. Decrease of serum total ghrelin in extensive atrophic gastritis:comparison with pepsinogens in histological reference[J]. Scand J Gastroenterol,2016,51(2):137-144.
[10]Shao Xiaohong. Correlation between Helicobacter pylori infection and pathological changes of gastric mucosa in patients with chronic gastritis[J]. Journal of Clinical and Experimental Medicine,2015,14(17):1416-1418.[邵晓红.幽门螺杆菌感染与慢性胃炎患者胃黏膜病理变化的相关性研究[J].临床和实验医学杂志,2015,14(17):1416-1418.]
[11]Zhu Bao,Yuan Hang,Xi Huifang,et al. Changes in CagA expression of gastric mucosa tissue in patients with Helicobacter pylori infective gastritis and changes in IL-6,IL-8 and CRP levels in the culture medium[J]. Chinese Journal of Infection Science,2011,21(2):236-238.[朱宝,袁航,奚惠芳,等.幽门螺杆菌感染性胃炎患者胃黏膜组织CagA表达与其培养液中IL-6、IL-8及CRP水平的变化[J].中华医院感染学杂志,2011,21(2):236-238.]
[12]Fatema J,Khan AH,Uddin MJ,et al. Chronic gastritis and its association with H. pylori infection[J]. Mymensingh Med J,2015,24(4):717-722.
[13]Xie Huizhong,Wang Li,Fu Zhongbao,et al. The relationship between the density of Helicobacter pylori infection and the expression of IL-6 and IL-8 and TNF-αin serum[J]. Medical Review,2015,21(1):142-144.[谢会忠,王莉,符仲标,等.幽门螺杆菌感染密度与血清IL-6和IL-8及TNF-α表达的关系[J].医学综述,2015,21(1):142-144.]
[14]Zhang Xiaoyan,Song Ying,Chu Youliang,et al. IL-10,COX-2in Helicobacter pylori related gastritis tissue expression and correlation study[J]. Journal of Gastroenterology and Hepatology,2011,20(9):804-808.[张小艳,宋瑛,楚有良,等. IL-10、COX-2在幽门螺杆菌相关性胃炎组织中的表达及相关性研究[J].胃肠病学和肝病学杂志,2011,20(9):804-808.]
[15]Bao Jianrong,Yao Weikang,Feng Yizhong,et al. Expression and correlation of PTEN,iNOS and COX-2 in colorectal cancer[J].Journal of Clinical and Experimental Pathology,2013,29(10):1069-1071.[包健荣,姚卫康,冯一中,等.大肠癌组织中PTEN、iNOS、COX-2的表达及相关性[J].临床与实验病理学杂志,2013,29(10):1069-1071.]
[16]Azadegan-Dehkordi F,Bagheri N,Shirzad M,et al. Correlation between mucosal IL-6 m RNA expression level and virulence eactors of helicobacter pylori in iranian adult patients with chronic gastritis[J]. Jundishapur J Microbiol,2015,8(8):21701-21702.
[17]Zhang Xiaoyan,Wang Shusheng,Song Ying,et al. Expressions of IL-10,iNOS in Helicobacter pylori related gastritis and precancerous lesions of gastric cancer[J]. Modern Oncology,2013,21(8):1830-1833.[张小艳,王曙升,宋瑛,等. IL-10、iNOS在幽门螺旋杆菌相关性胃炎及胃癌前病变组织中表达的相关性研究[J].现代肿瘤医学,2013,21(8):1830-1833.]
[18]Guo Xuan,Luo Yan,Yu Bin,et al. The effect of Tang Yin on AQP4,IL-6 and IL-8 in mice with Helicobacter pylori associated gastritis,spleen and stomach damp heat syndrome[J]. Chinese Medicine Emergency,2014,23(9):1599-1601,1679.[郭璇,罗燕,喻斌,等.灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证小鼠AQP4、IL-6、IL-8的影响[J].中国中医急症,2014,23(9):1599-1601,1679.]
[19]Mujawar P,Nikumbh DB,Suryawanshi KH,et al. Helicobacter pylori associated gastritis in Northern Maharashtra,India:A histopathological study of gastric mucosal biopsies[J]. J Clin Diagn Res,2015,9(6):04-06.
[20]Jiang Yan,Song Chengyue,Wang Guizhou,et al. Expression of TNF-alpha,IL-1 beta and iNOS in gastric mucosa of patients with Helicobacter pylori positive chronic gastritis[J]. Journal of Bengbu Medical College,2012,37(5):556-558.[江燕,宋澄跃,王桂周,等.幽门螺杆菌阳性的慢性胃炎患者胃黏膜组织中TNF-α、IL-1β、iNOS的表达[J].蚌埠医学院学报,2012,37(5):556-558.]
[2]Sun Bo. The relationship between the expression of tumor necrosis factor-alpha,interleukin 1 beta and inducible nitric oxide synthase and Helicobacter pylori associated gastritis[J]. Chinese General Medicine,2010,13(11):1155-1157.[孙波.肿瘤坏死因子α和白介素1β及诱导型一氧化氮合酶的表达与幽门螺杆菌相关性胃炎的关系[J].中国全科医学,2010,13(11):1155-1157.]
[3]Zhang Guosheng. Effect of triple therapy on serum IL-10,TGFbeta 1 and IL-17 levels in elderly patients with Helicobacter pylori gastritis[J]. Chongqing Medicine,2014,43(7):821-822,825.[张国胜.三联疗法对老年幽门螺杆菌胃炎患者血清IL-10、TGF-β1及IL-17水平的影响[J].重庆医学,2014,43(7):821-822,825.]
[4]Ma Jian,Meng Xinying,Wang Tao,et al. The relationship and clinical significance of serum IL-6,TGF-beta 1 and IL-17 with Helicobacter pylori in patients with chronic active gastritis[J]. Chinese Clinician Journal(Electronic Edition),2012,06(11):2908-2910.[马健,孟欣颖,王涛,等.慢性活动性胃炎患者血清IL-6、TGF-β1及IL-17的水平与幽门螺杆菌的关系及临床意义[J].中华临床医师杂志(电子版),2012,06(11):2908-2910.]
[5]Peng Huajun,Wu Xiaowei. Expression of CRP level in Helicobacter pylori positive gastritis[J]. Journal of Clinical and Experimental Medicine,2015,14(2):89-91.[彭华军,吴小微. IL-8、IL-10及CRP水平在幽门螺杆菌阳性胃炎患者中的表达[J].临床和实验医学杂志,2015,14(2):89-91.]
[6]Wu Juan. The level and clinical significance of interleukin-10and interleukin-17 in serum of patients with acute gastritis[J].Chinese Journal of Medical Education,2012,35(34):32-34.[吴娟.白细胞介素-10和白细胞介素-17在急性胃炎患者血清中的水平及临床意义[J].中国医师进修杂志,2012,35(34):32-34.]
[7]Wang Jian,Hao Haitao,Li Shuxia,et al. The effect of triple therapy on Hp(+)chronic superficial gastritis and its effect on the changes of IL-4,IFN-gamma[J]. Modern Digestion and Interventional Diagnosis and Treatment,2015,20(4):425-427.[王剑,郝海涛,李淑霞,等.三联疗法对Hp(+)慢性浅表性胃炎的疗效及其对IL-4、IFN-γ水平变化的影响[J].现代消化及介入诊疗,2015,20(4):425-427.]
[8]Chinese Society of Gastroenterology,Chinese Medical Association.National consensus on chronic gastritis Symposium[J]. Chinese Journal of Digestion,2000,20(3):199-201.[中华医学会消化病学分会.全国慢性胃炎研讨会共识意见[J].中华消化杂志,2000,20(3):199-201.]
[9]Eun Bae S,Hoon Lee J,Soo Park Y,et al. Decrease of serum total ghrelin in extensive atrophic gastritis:comparison with pepsinogens in histological reference[J]. Scand J Gastroenterol,2016,51(2):137-144.
[10]Shao Xiaohong. Correlation between Helicobacter pylori infection and pathological changes of gastric mucosa in patients with chronic gastritis[J]. Journal of Clinical and Experimental Medicine,2015,14(17):1416-1418.[邵晓红.幽门螺杆菌感染与慢性胃炎患者胃黏膜病理变化的相关性研究[J].临床和实验医学杂志,2015,14(17):1416-1418.]
[11]Zhu Bao,Yuan Hang,Xi Huifang,et al. Changes in CagA expression of gastric mucosa tissue in patients with Helicobacter pylori infective gastritis and changes in IL-6,IL-8 and CRP levels in the culture medium[J]. Chinese Journal of Infection Science,2011,21(2):236-238.[朱宝,袁航,奚惠芳,等.幽门螺杆菌感染性胃炎患者胃黏膜组织CagA表达与其培养液中IL-6、IL-8及CRP水平的变化[J].中华医院感染学杂志,2011,21(2):236-238.]
[12]Fatema J,Khan AH,Uddin MJ,et al. Chronic gastritis and its association with H. pylori infection[J]. Mymensingh Med J,2015,24(4):717-722.
[13]Xie Huizhong,Wang Li,Fu Zhongbao,et al. The relationship between the density of Helicobacter pylori infection and the expression of IL-6 and IL-8 and TNF-αin serum[J]. Medical Review,2015,21(1):142-144.[谢会忠,王莉,符仲标,等.幽门螺杆菌感染密度与血清IL-6和IL-8及TNF-α表达的关系[J].医学综述,2015,21(1):142-144.]
[14]Zhang Xiaoyan,Song Ying,Chu Youliang,et al. IL-10,COX-2in Helicobacter pylori related gastritis tissue expression and correlation study[J]. Journal of Gastroenterology and Hepatology,2011,20(9):804-808.[张小艳,宋瑛,楚有良,等. IL-10、COX-2在幽门螺杆菌相关性胃炎组织中的表达及相关性研究[J].胃肠病学和肝病学杂志,2011,20(9):804-808.]
[15]Bao Jianrong,Yao Weikang,Feng Yizhong,et al. Expression and correlation of PTEN,iNOS and COX-2 in colorectal cancer[J].Journal of Clinical and Experimental Pathology,2013,29(10):1069-1071.[包健荣,姚卫康,冯一中,等.大肠癌组织中PTEN、iNOS、COX-2的表达及相关性[J].临床与实验病理学杂志,2013,29(10):1069-1071.]
[16]Azadegan-Dehkordi F,Bagheri N,Shirzad M,et al. Correlation between mucosal IL-6 m RNA expression level and virulence eactors of helicobacter pylori in iranian adult patients with chronic gastritis[J]. Jundishapur J Microbiol,2015,8(8):21701-21702.
[17]Zhang Xiaoyan,Wang Shusheng,Song Ying,et al. Expressions of IL-10,iNOS in Helicobacter pylori related gastritis and precancerous lesions of gastric cancer[J]. Modern Oncology,2013,21(8):1830-1833.[张小艳,王曙升,宋瑛,等. IL-10、iNOS在幽门螺旋杆菌相关性胃炎及胃癌前病变组织中表达的相关性研究[J].现代肿瘤医学,2013,21(8):1830-1833.]
[18]Guo Xuan,Luo Yan,Yu Bin,et al. The effect of Tang Yin on AQP4,IL-6 and IL-8 in mice with Helicobacter pylori associated gastritis,spleen and stomach damp heat syndrome[J]. Chinese Medicine Emergency,2014,23(9):1599-1601,1679.[郭璇,罗燕,喻斌,等.灭幽汤对幽门螺杆菌相关性胃炎脾胃湿热证小鼠AQP4、IL-6、IL-8的影响[J].中国中医急症,2014,23(9):1599-1601,1679.]
[19]Mujawar P,Nikumbh DB,Suryawanshi KH,et al. Helicobacter pylori associated gastritis in Northern Maharashtra,India:A histopathological study of gastric mucosal biopsies[J]. J Clin Diagn Res,2015,9(6):04-06.
[20]Jiang Yan,Song Chengyue,Wang Guizhou,et al. Expression of TNF-alpha,IL-1 beta and iNOS in gastric mucosa of patients with Helicobacter pylori positive chronic gastritis[J]. Journal of Bengbu Medical College,2012,37(5):556-558.[江燕,宋澄跃,王桂周,等.幽门螺杆菌阳性的慢性胃炎患者胃黏膜组织中TNF-α、IL-1β、iNOS的表达[J].蚌埠医学院学报,2012,37(5):556-558.]