响应面法优化蒙药瞿麦中总生物碱提取工艺研究
|
摘要
研究了响应面法优化蒙药瞿麦总生物碱的最佳提取工艺。采用紫外-可见分光光度法,以总生物碱含量作为评价指标,用Box-Behnken响应面设计考察各因素对其影响,对结果进行二项式拟合及响应曲面分析得到最佳提取工艺。结果表明:蒙药瞿麦中总生物碱的最佳提取工艺为超声温度53℃,料液比1∶31 g/mL,超声时间41 min,超声功率140 W。四因素影响顺序为料液比>超声温度>超声功率>超声时间。此方法简便、快速、灵敏,结果准确可靠,优化后的提取工艺稳定可行,且具有提取率高、提取时间短等优点,对今后的科研工作有一定参考价值。
Response surface method optimizes optimum extraction process method of total alkaloids from Mongolian medicine dianthus superbus L.UV-vis spectrophotometry was used to measure the content of total alkaloid,which was used as the evaluation index to determine the optimum technological conditions with Box-Behnken response surface design,binomial fitting and response surface analysis.The optimum ultrasonic extraction process of total alkaloid from Mongolian medicine dianthus superbus L.was ultrasonic temperature 53℃,solid-liquid ratio 1∶31 g/mL,ultrasonic time 41 minutes,ultrasonic power 140 W.The effect order of four factors was solid-liquid ratio>ultrasonic temperature>ultrasonic power>ultrasonic time.The method was convenient,rapid,sensitive,accurate,which had high extraction rate and provided reference value for the future research.
Response surface method optimizes optimum extraction process method of total alkaloids from Mongolian medicine dianthus superbus L.UV-vis spectrophotometry was used to measure the content of total alkaloid,which was used as the evaluation index to determine the optimum technological conditions with Box-Behnken response surface design,binomial fitting and response surface analysis.The optimum ultrasonic extraction process of total alkaloid from Mongolian medicine dianthus superbus L.was ultrasonic temperature 53℃,solid-liquid ratio 1∶31 g/mL,ultrasonic time 41 minutes,ultrasonic power 140 W.The effect order of four factors was solid-liquid ratio>ultrasonic temperature>ultrasonic power>ultrasonic time.The method was convenient,rapid,sensitive,accurate,which had high extraction rate and provided reference value for the future research.
引文
[1]国家药典委员会编.中华人民共和国药典2015年版[S].北京:中国医药科技出版社,2015:382-383.
[2]陈建平,王金辉,汤化琪,等.蒙药瞿麦中多糖超声提取工艺研究及含量测定[J].中南药学,2013,11(4):264-267.
[3]张德华,黄仁术,左露,等.生物碱的提取方法研究进展[J].中国野生植物资源,2010,29(5):15-19.
[4]王来兵,马岚,白文明,等.蒙药瞿麦中总生物碱含量测定[J].疾病监测与控制,2018,12(3):174-176.
[5]马燮,杨郭,林艳,等.微波预处理提取蛇足石杉中总生物碱的工艺优化[J].化学研究与应用,2009,21(8):1 213-1 216
[6]张海容,白娟,魏增云,等.超声萃取-响应面法优化淡竹叶多糖提取方法研究[J].化学研究与应用,2013,25(3):303-310.
[7]贺胜,周杏子,何海,等.响应面法优化桑叶总生物碱超声提取工艺[J].中国药房,2015,26(25):3 537-3 540.
[8]尹斐,杨洁红,方雨晨,等.基于2种分析方法的补阳还五汤中有效成分提取工艺优化研究[J].中草药,2018,49(1):135-141.
[9]王峥,曾凡荣,舒俊翔,等.响应面分析法优化金槐总黄酮的提取工艺[J].广州化工,2017,45(20):82-85.
[2]陈建平,王金辉,汤化琪,等.蒙药瞿麦中多糖超声提取工艺研究及含量测定[J].中南药学,2013,11(4):264-267.
[3]张德华,黄仁术,左露,等.生物碱的提取方法研究进展[J].中国野生植物资源,2010,29(5):15-19.
[4]王来兵,马岚,白文明,等.蒙药瞿麦中总生物碱含量测定[J].疾病监测与控制,2018,12(3):174-176.
[5]马燮,杨郭,林艳,等.微波预处理提取蛇足石杉中总生物碱的工艺优化[J].化学研究与应用,2009,21(8):1 213-1 216
[6]张海容,白娟,魏增云,等.超声萃取-响应面法优化淡竹叶多糖提取方法研究[J].化学研究与应用,2013,25(3):303-310.
[7]贺胜,周杏子,何海,等.响应面法优化桑叶总生物碱超声提取工艺[J].中国药房,2015,26(25):3 537-3 540.
[8]尹斐,杨洁红,方雨晨,等.基于2种分析方法的补阳还五汤中有效成分提取工艺优化研究[J].中草药,2018,49(1):135-141.
[9]王峥,曾凡荣,舒俊翔,等.响应面分析法优化金槐总黄酮的提取工艺[J].广州化工,2017,45(20):82-85.