基于色差原理分析龙胆有效成分含量与颜色的相关性
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摘要
目的:通过测定龙胆色差值及有效成分龙胆苦苷的含量,将代表颜色的外观指标值与代表质量的内在含量指标值相关联,探讨有效成分含量与颜色之间的相关性,为常用中药龙胆的质量评价提供参考依据。方法:利用色差仪对龙胆粉末进行测定,以2015年版《中国药典》中龙胆含量测定项下方法,采用HPLC进行测定,通过SPSS 21. 0软件进行统计分析。结果:龙胆中龙胆苦苷含量与L*(代表颜色深浅),E*ab(色差值)存在极显著正相关关系(P <0. 01),表明在一定程度上,L*,E*ab值越大,龙胆苦苷含量越高;而龙胆苦苷含量与a*(代表颜色红绿色度),b*(代表颜色黄蓝色度)存在极显著负相关关系(P <0. 01),表明在一定程度上,a*,b*值越小,龙胆苦苷含量越高。结论:龙胆外观颜色与有效成分龙胆苦苷含量有关,且颜色偏浅黄色的龙胆有效成分含量较高,质量相对较好。通过色差仪对龙胆色差值进行测定,快速预测龙胆中有效成分的含量,可为该药材质量评价体系的建立提供参考依据。
Objective: To measure the color values of Gentianae Radix et Rhizoma and the contents of gentiopicroside,the appearance index value of the representative color was correlated with the intrinsic index value representing the quality,in order to explore the correlation between the contents of active ingredients and the color values,and provide basis for the quality evaluation of Gentianae Radix et Rhizoma. Method: The color difference values of Gentianae Radix et Rhizoma powder was measured by colorimeter. The content determination method of gentiopicroside in the 2015 edition of China Pharmacopoeia was adopted. The content of gentiopicroside was determined by HPLC,and the correlation and regression analysis was carried out by SPSS 21. 0 software. Result:There was a significantly positive correlation between the contents of gentiopicroside and L*( representing colorshade) and E*ab( representing total color difference)( P < 0. 01),while the contents of gentiopicroside and a*( representing color red-green direction) and b*( representing color yellow-blue direction) had a significantly negative correlation( P < 0. 01). Conclusion: The color value of Gentianae Radix et Rhizoma has a certain correlation with the content of gentiopicroside,and yellowish Gentianae Radix et Rhizoma contain more active ingredients,with a better quality. The contents of gentiopicroside active ingredients in Gentianae Radix et Rhizoma can be quickly predicted by determining the color difference values,which can provide a new idea for quality evaluation of this herb.
Objective: To measure the color values of Gentianae Radix et Rhizoma and the contents of gentiopicroside,the appearance index value of the representative color was correlated with the intrinsic index value representing the quality,in order to explore the correlation between the contents of active ingredients and the color values,and provide basis for the quality evaluation of Gentianae Radix et Rhizoma. Method: The color difference values of Gentianae Radix et Rhizoma powder was measured by colorimeter. The content determination method of gentiopicroside in the 2015 edition of China Pharmacopoeia was adopted. The content of gentiopicroside was determined by HPLC,and the correlation and regression analysis was carried out by SPSS 21. 0 software. Result:There was a significantly positive correlation between the contents of gentiopicroside and L*( representing colorshade) and E*ab( representing total color difference)( P < 0. 01),while the contents of gentiopicroside and a*( representing color red-green direction) and b*( representing color yellow-blue direction) had a significantly negative correlation( P < 0. 01). Conclusion: The color value of Gentianae Radix et Rhizoma has a certain correlation with the content of gentiopicroside,and yellowish Gentianae Radix et Rhizoma contain more active ingredients,with a better quality. The contents of gentiopicroside active ingredients in Gentianae Radix et Rhizoma can be quickly predicted by determining the color difference values,which can provide a new idea for quality evaluation of this herb.
引文
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[2]沈涛,金航,王元忠,等.中药龙胆化学成分研究进展[J].安徽农业科学,2010,38(30):16868-16870,16874.
[3]王艳艳,王英平,王晓杰,等.龙胆化学成分及药理作用研究进展[J].特产研究,2006(3):68-71.
[4]杨书彬,王承.龙胆化学成分和药理作用研究进展[J].中医药学报,2005,33(6):54-56.
[5]万德光.论中药品质理论的继承与创新[J].中药与临床,2010,1(1):3-6.
[6]徐珍珍,史星星,樊旭蕾,等.基于色差原理分析木香有效成分含量与颜色值的相关性[J].中国实验方剂学杂志,2018,24(13):17-21.
[7]郭换,刘飞,梅国荣,等.色度分析花椒黄酮类成分含量与颜色值的相关性[J].中国实验方剂学杂志,2017,23(6):91-97.
[8]马婷婷,龚慕辛,王智民,等.甘草色泽与有效成分含量的相关性研究[J].中国中药杂志,2017,42(19):3776-3785.
[9]赵雷蕾,周洋,黎茂,等.基于数据化表达的中药“形色气味”研究进展及思考[J].广东药学院学报,2015,31(5):692-695.
[10]杨添钧.基于机器视觉技术的药材及饮片“辨色”研究[D].成都:成都中医药大学,2014.
[11]黎江华,吴纯洁,孙灵根,等.基于机器视觉技术实现中药性状“形色”客观化表达的展望[J].中成药,2011,33(10):1781-1784.