生髓健骨胶囊对酒精性骨质疏松大鼠骨密度、骨矿含量影响的研究
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摘要
目的从基因分子水平探讨酒精性骨质疏松(alcoholic osteoporosis,AOP)大鼠的发病机制,观察生髓健骨胶囊对AOP大鼠骨密度(bone mineral density,BMD)、骨矿含量(bone mineral content,BMC)表达的影响,探讨生髓健骨胶囊对AOP大鼠模型的中药防治作用机理。方法选取成年雄性(清洁级)SD大鼠120只,称体重,随机分为4组,每组各30只,用白酒灌胃法造模,同时分别给予生理盐水、碳酸钙阿法D3、生髓健骨胶囊灌胃给药。于造模8、12、16周末取材,检测股骨上端BMD、BMC指标。结果检测造模干预8、12、16周后BMD、BMC指标变化,模型组BMD、BMC与正常组比较明显降低,且差异有统计学意义(P<0.01),结果表明饮酒大鼠确实存在骨量减少,BMD、BMC降低;中药干预组BMD、BMC与模型组相比显著升高(P<0.01);中药干预组BMD、BMC与西药对照组相比,明显升高(P<0.05)。结论通过观察生髓健骨胶囊对AOP大鼠的实验指标,证明了生髓健骨胶囊能够提高AOP大鼠骨密度,增加骨矿含量,抑制矿物质丢失,改善大鼠的骨代谢。
Objective To explore the pathogenesis of alcohol-induced osteoporosis(AOP) in rats from the molecular gene level, to observe the effect of the generating marrow and strong-bone capsule on bone mineral density(BMD) and bone mineral content(BMC) in AOP rats, and to discuss the preventive and therapeutic mechanism of generating marrow and strong-bone capsule in AOP rats. Methods One hundred and twenty adult male SD rats(clean grade) were weighted and randomly divided into 4 groups, with 30 rats in each group. The rats were modelled with Red Star Erguotou liquor gavage method. At the same time, normal saline, calcium carbonate alfa-D3 and generating marrow and strong-bone capsule were gavaged, respectively. On 8, 12, and 16 weeks, BMD and BMC of the upper femur were detected. Results Software SPSS 22.0 was used for statistical analysis(P<0.05) and t test was performed. The changes of BMD and BMC at 8, 12 and 16 weeks after modeling intervention were detected. BMD and BMC in model group and normal group decreased significantly, and there was significant difference(P<0.01). The result showed that there was a decrease in bone mass and BMD and BMC in rats taking alcohol. Compared with model group, BMD and BMC in Chinese medicine intervention group increased significantly(P<0.01). Compared with western medicine control group, BMD and BMC increased significantly(P<0.05). Conclusion By observing the experimental index of the generating marrow and strong-bone capsule on AOP rats, it is proved that the generating marrow and strong-bone capsule increases BMD and BMC, inhibits mineral loss, and improves bone metabolism in AOP rats.
Objective To explore the pathogenesis of alcohol-induced osteoporosis(AOP) in rats from the molecular gene level, to observe the effect of the generating marrow and strong-bone capsule on bone mineral density(BMD) and bone mineral content(BMC) in AOP rats, and to discuss the preventive and therapeutic mechanism of generating marrow and strong-bone capsule in AOP rats. Methods One hundred and twenty adult male SD rats(clean grade) were weighted and randomly divided into 4 groups, with 30 rats in each group. The rats were modelled with Red Star Erguotou liquor gavage method. At the same time, normal saline, calcium carbonate alfa-D3 and generating marrow and strong-bone capsule were gavaged, respectively. On 8, 12, and 16 weeks, BMD and BMC of the upper femur were detected. Results Software SPSS 22.0 was used for statistical analysis(P<0.05) and t test was performed. The changes of BMD and BMC at 8, 12 and 16 weeks after modeling intervention were detected. BMD and BMC in model group and normal group decreased significantly, and there was significant difference(P<0.01). The result showed that there was a decrease in bone mass and BMD and BMC in rats taking alcohol. Compared with model group, BMD and BMC in Chinese medicine intervention group increased significantly(P<0.01). Compared with western medicine control group, BMD and BMC increased significantly(P<0.05). Conclusion By observing the experimental index of the generating marrow and strong-bone capsule on AOP rats, it is proved that the generating marrow and strong-bone capsule increases BMD and BMC, inhibits mineral loss, and improves bone metabolism in AOP rats.
引文
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[18] 任树军,邢国利,姜益常,等.补肾健脾益气法对酒精性骨质疏松大鼠骨密度(BMD)、骨矿含量(BMC)影响的研究[J].中医药学报,2013,41(1):24-26.
[2] 贺丽英,孙蕴,要文娟,等.2010～2016年中国老年人骨质疏松症患病率Meta分析[J].中国骨质疏松杂志,2016,22(12):1590-1596.
[3] 王冰梅,赵娜,于洪宇,等.骨质疏松的中西医研究进展[J].中医药信息,2016,33(6):128-131.
[4] 杨颜茹,郭强,段惠春.IL-6、TNT-α、1,25二羟维生素D_3以及骨密度测定在酒精性肝硬化骨质疏松中的临床应用价值[J].甘肃医药,2013,32(3):163-166.
[5] 任树军,于雪峰,孙贵才,等.酒精性骨质疏松症发病机制的研究进展[J].中国骨质疏松杂志,2008,14(8):601-604.
[6] 胡细连.酒精性骨质疏松的研究现况分析[J].中外医学研究,2017,15(17):162-164.
[7] 杨志鹏,魏成建,龚双全.骨质疏松症的中医治疗研究进展[J].中国骨质疏松杂志,2015,21(11):1381-1384.
[8] 赵志强,阎晓霞.中药补肾法改善原发性骨质疏松症临床症状的研究[J].中国骨质疏松杂志,2018,24(3):371-375,410.
[9] 柳承希,任艳玲.古代文献对骨质疏松症的认识[J].中华中医药杂志,2014,29(7):2089-2092.
[10] 肖达民,李丹青,吴艳华.酒精性肝病的中医临床研究进展[J].中药新药与临床药理,2018,29(1):118-122.
[11] 任树军,邢国利,于雪峰,等.酒精性骨质疏松症的研究概况[J].中医药信息,2008,25(4):12-15.
[12] 王伟,万雷,柴爽,等.骨质疏松症的中医病因病机和分期治疗[J].中医正骨,2018,30(2):29-30.
[13] 赖满香,廖利平,谭玮璐,等.“肾精-骨质疏松-骨髓间充质干细胞”理论探讨[J].中医杂志,2018,59(2):100-103.
[14] 李祥雨,姜劲挺,李建国,等.骨质疏松症中药防治研究进展[J].中国骨质疏松杂志,2018,24(2):270-275.
[15] 曹毅.中药葛根的相关药理药效研究综述[J].中国处方药,2018,16(2):27-28.
[16] Ren SJ,Xing GL,Hu NW,et al.BuShenJianPiYiQi Therapy Prevent Alcohol-Induced Osteoporosis in Rats[J].American Journal of Therapeutics,2016,23(5):1135-1142.
[17] 任树军,邢国利,葛明富,等.补肾健脾益气法对酒精性骨质疏松大鼠小肠中VDR-mRNA表达影响的研究[J].中医药信息,2013,30(2):46-49.
[18] 任树军,邢国利,姜益常,等.补肾健脾益气法对酒精性骨质疏松大鼠骨密度(BMD)、骨矿含量(BMC)影响的研究[J].中医药学报,2013,41(1):24-26.