基于16S rRNA基因高通量测序的太原地区健康成年人肠道菌群结构分析
|
摘要
目的探讨太原地区健康成年人的肠道菌群结构及多样性。方法采集太原地区20份健康成年人的粪便样本,提取DNA、构建菌群16S rRNA基因克隆文库、高通量测序并进行生物信息学分析。结果测序获得优质序列1 383 680条,平均序列为69 184条±551条,归类于455个OTUs;所有样本共含有10个菌门,101个菌属,141种菌;其中核心菌门3个,依次为拟杆菌门(63.13%)、厚壁菌门(31.14%)和变形菌门(5.10%),占所有样本微生物总量的99.37%;丰度最高的前10个菌属依次为拟杆菌属(43.34%)、普氏菌属(15.51%)、栖粪杆菌属(4.92%)、罗氏菌属(4.73%)、毛螺菌属(3.27%)、萨特菌属(2.50%)、粪球菌属(2.38%)、布劳特菌属(2.31%)、瘤胃球菌属(2.18%)和副杆状菌属(1.61%),占样本微生物总量的82.75%,未分类的菌属占6.84%。结论健康成年人肠道微生物群落复杂,但主要菌群相对稳定,为进一步研究人体肠道菌群结构与功能提供了参考依据。
Objective To explore the structure and diversity of gut microflora of healthy adults in Taiyuan area. Methods Twenty cases fecal samples of healthy adults in Taiyuan area were collected, DNA of these fecal samples was extracted, the 16 S rRNA gene clone library of bacteria was constructed, high-throughput sequencing was conducted, and bioinformatics analysis was conducted. Results High quality sequences were obtained(1,383,680), with an average of 69 184±551 sequences, which were classified into 455 OTUs. All the samples involved 10 phyla, 101 genera and 141 species of microorganisms. Bacteroidetes, Firmicutes and Proteobacteria were the core bacteria, accounting for 63.13%, 31.14% and 5.10% respectively. The top 10 genera were Bacteroides(43.34%), Prevotella(15.51%), Faecalibacterium(4.92%), Roseburia(4.73%), Lachnospira(3.27%), Sutterella(2.50%), Coprococcus(2.38%), Blautia(2.31%), Ruminococcus(2.18%) and Parabacteroides(1.61%), accounting for 82.75% of the total microbial species, and 6.84% of unclassified species. Conclusion The gut microflora of healthy adults are complex, but the main microflora are relatively stable. This result provides a reference for further study on the structure and function of human gut microflora.
Objective To explore the structure and diversity of gut microflora of healthy adults in Taiyuan area. Methods Twenty cases fecal samples of healthy adults in Taiyuan area were collected, DNA of these fecal samples was extracted, the 16 S rRNA gene clone library of bacteria was constructed, high-throughput sequencing was conducted, and bioinformatics analysis was conducted. Results High quality sequences were obtained(1,383,680), with an average of 69 184±551 sequences, which were classified into 455 OTUs. All the samples involved 10 phyla, 101 genera and 141 species of microorganisms. Bacteroidetes, Firmicutes and Proteobacteria were the core bacteria, accounting for 63.13%, 31.14% and 5.10% respectively. The top 10 genera were Bacteroides(43.34%), Prevotella(15.51%), Faecalibacterium(4.92%), Roseburia(4.73%), Lachnospira(3.27%), Sutterella(2.50%), Coprococcus(2.38%), Blautia(2.31%), Ruminococcus(2.18%) and Parabacteroides(1.61%), accounting for 82.75% of the total microbial species, and 6.84% of unclassified species. Conclusion The gut microflora of healthy adults are complex, but the main microflora are relatively stable. This result provides a reference for further study on the structure and function of human gut microflora.
引文
[1] Gareau MG,Sherman PM,Walker WA.Probiotics and the gut microbiota in intestinal health and disease[J].Nat Rev Gastroenterol & Hepatol,2010,7(9):503-514.
[2] Turnbaugh PJ,Hamady M,Yatsunenko T,et al.A core gut microbiome in obese and lean twins[J].Nature,2008,457(7228):480-484.
[3] Matsumoto M,Kibe R,Ooga T,et al.Impact of intestinalmicrobiota on intestinal luminal metabolome[J].Sci Rep,2012,2:233.
[4] Mazmanian SK,Liu CH,Tzianabos AO,et al.An immunomodulatory molecule of symbiotic bacteria directs maturation of the host immune system[J].Cell,2005,122(1):107-118.
[5] ZENG Xueqin,CHEN Yue,ZHANG Xiangzhuo,et al.Research advances in fecal microbiota transplantation for diseases associated with gut dysbiosis[J].J Hunan Univ Chin Med,2019,39(1):127-131.(in Chinese) 曾雪芹,陈悦,张湘卓,等.粪菌移植治疗肠道微生态失调所致疾病的研究进展[J].湖南中医药大学学报,2019,39(1):127-131.
[6] WANG Rong,SHAO Yiyan,LIN Jiajia,et al.The involvement and application of gut microbiota and probiotics in aging[J].Chin Bull Life Sci,2019,31(1):80-84.(in Chinese) 王融,邵祎妍,林佳佳,等.肠道菌群与益生菌在衰老及其调控中的研究与应用[J].生命科学,2019,31(1):80-84.
[7] Claesson MJ,Cusack S,O′Sullivan O,et al.Composition,variability,and temporal stability of the intestinal microbiota of the elderly[J].Proc Natl Acad Sci U S A,2011,108(Suppl 1):4586-4591.
[8] Mueller S,Saunier K,Hanisch C,et al.Differences in fecal microbiota in different European study populations in relation to age,gender,and country:a cross-sectional study[J].Appl Environ Microbiol,2006,72(2):1027-1033.
[9] ZHANG Jiachao.Mongolians core gut microbiota and its correlation with seasonal dietary changes[D].Hohhot:Inner Mongolia Agricultural University,2014.(in Chinese) 张家超.蒙古族肠道核心菌群及其与饮食关联性研究[D].呼和浩特:内蒙古农业大学,2014.
[10] Arumugam M,Raes J,Pelletier E,et al.Enterotypes of the human gut microbiome[J].Nature,2011,473(7346):174-180.
[11] Nam YD,Jung MJ,Roh SW,et al.Comparative analysis of Korean human gut microbiota by barcoded pyrosequencing[J].PLoS One,2011,6(7):e22109.
[12] Liu WJ,Zhang JC,Wu CY,et al.Unique features of ethnic Mongolian gut microbiome revealed by metagenomic analysis[J].Sci Rep,2016,6:34826.
[13] WU Na,ZHU Baoli.Human gut microbiota and healthy[J].Science,2013,65(2):13-15.(in Chinese) 武娜,朱宝利.人体肠道微生物与健康[J].科学,2013,65(2):13-15.
[14] ZHAI Qixiao,TIAN Fengwei,WANG Gang,et al.Progress in research on the role of intestinal microbiota in human health[J].Food Sci,2013,34(15):337-341.(in Chinese) 翟齐啸,田丰伟,王刚,等.肠道微生物与人体健康的研究进展[J].食品科学,2013,34(15):337-341.
[15] De Filippo C,Cavalieri D,Di Paola M,et al.Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa[J].Proc Natl Acad Sci U S A,2010,107(33):14691-14696.
[16] ZHAO Liping,ZHANG Chenhong.Functional dynamics of gut microbiota in obesity and metabolic diseases[J].Chin Bull Life Sci,2010,22(12):1247-1253.(in Chinese) 赵立平,张晨虹.肥胖相关的肠道微生物群落结构动力学与功能解析研究[J].生命科学,2010,22(12):1247-1253.
[17] Martinez I,Muller CE,Walter J.Long-term temporal analysis of the human fecal microbiota revealed a stable core of dominant bacterial species[J].PLoS One,2013,8(7):69621.
[18] ZANG Kaili,JIANG Yan,SUN Yong.et al.Relationship between microecologics and the expression of short chain fatty acids synthesis genes in key bacterial genera in the regulation of intestinal flora structure in populations with constipation and diarrhea[J].Food Sci,2018,39(5):155-165.(in Chinese) 臧凯丽,江岩,孙勇,等.微生态制剂调节便秘、腹泻人群肠道菌群结构与产短链脂肪酸关键菌属的相关性[J].食品科学,2018,39(5):155-165.
[19] PENG Qiannan,HUO Dongxue,XU Chuanbiao,et al.Intestinal microbiota in Li cohort and its correlation with their diets[J].Microbiol China,2017,44(11):2624-2633.(in Chinese) 彭倩楠,霍冬雪,徐传标,等.黎族人肠道微生物群落结构特征及其与饮食关联性[J].微生物学通报,2017,44(11):2624-2633.
[2] Turnbaugh PJ,Hamady M,Yatsunenko T,et al.A core gut microbiome in obese and lean twins[J].Nature,2008,457(7228):480-484.
[3] Matsumoto M,Kibe R,Ooga T,et al.Impact of intestinalmicrobiota on intestinal luminal metabolome[J].Sci Rep,2012,2:233.
[4] Mazmanian SK,Liu CH,Tzianabos AO,et al.An immunomodulatory molecule of symbiotic bacteria directs maturation of the host immune system[J].Cell,2005,122(1):107-118.
[5] ZENG Xueqin,CHEN Yue,ZHANG Xiangzhuo,et al.Research advances in fecal microbiota transplantation for diseases associated with gut dysbiosis[J].J Hunan Univ Chin Med,2019,39(1):127-131.(in Chinese) 曾雪芹,陈悦,张湘卓,等.粪菌移植治疗肠道微生态失调所致疾病的研究进展[J].湖南中医药大学学报,2019,39(1):127-131.
[6] WANG Rong,SHAO Yiyan,LIN Jiajia,et al.The involvement and application of gut microbiota and probiotics in aging[J].Chin Bull Life Sci,2019,31(1):80-84.(in Chinese) 王融,邵祎妍,林佳佳,等.肠道菌群与益生菌在衰老及其调控中的研究与应用[J].生命科学,2019,31(1):80-84.
[7] Claesson MJ,Cusack S,O′Sullivan O,et al.Composition,variability,and temporal stability of the intestinal microbiota of the elderly[J].Proc Natl Acad Sci U S A,2011,108(Suppl 1):4586-4591.
[8] Mueller S,Saunier K,Hanisch C,et al.Differences in fecal microbiota in different European study populations in relation to age,gender,and country:a cross-sectional study[J].Appl Environ Microbiol,2006,72(2):1027-1033.
[9] ZHANG Jiachao.Mongolians core gut microbiota and its correlation with seasonal dietary changes[D].Hohhot:Inner Mongolia Agricultural University,2014.(in Chinese) 张家超.蒙古族肠道核心菌群及其与饮食关联性研究[D].呼和浩特:内蒙古农业大学,2014.
[10] Arumugam M,Raes J,Pelletier E,et al.Enterotypes of the human gut microbiome[J].Nature,2011,473(7346):174-180.
[11] Nam YD,Jung MJ,Roh SW,et al.Comparative analysis of Korean human gut microbiota by barcoded pyrosequencing[J].PLoS One,2011,6(7):e22109.
[12] Liu WJ,Zhang JC,Wu CY,et al.Unique features of ethnic Mongolian gut microbiome revealed by metagenomic analysis[J].Sci Rep,2016,6:34826.
[13] WU Na,ZHU Baoli.Human gut microbiota and healthy[J].Science,2013,65(2):13-15.(in Chinese) 武娜,朱宝利.人体肠道微生物与健康[J].科学,2013,65(2):13-15.
[14] ZHAI Qixiao,TIAN Fengwei,WANG Gang,et al.Progress in research on the role of intestinal microbiota in human health[J].Food Sci,2013,34(15):337-341.(in Chinese) 翟齐啸,田丰伟,王刚,等.肠道微生物与人体健康的研究进展[J].食品科学,2013,34(15):337-341.
[15] De Filippo C,Cavalieri D,Di Paola M,et al.Impact of diet in shaping gut microbiota revealed by a comparative study in children from Europe and rural Africa[J].Proc Natl Acad Sci U S A,2010,107(33):14691-14696.
[16] ZHAO Liping,ZHANG Chenhong.Functional dynamics of gut microbiota in obesity and metabolic diseases[J].Chin Bull Life Sci,2010,22(12):1247-1253.(in Chinese) 赵立平,张晨虹.肥胖相关的肠道微生物群落结构动力学与功能解析研究[J].生命科学,2010,22(12):1247-1253.
[17] Martinez I,Muller CE,Walter J.Long-term temporal analysis of the human fecal microbiota revealed a stable core of dominant bacterial species[J].PLoS One,2013,8(7):69621.
[18] ZANG Kaili,JIANG Yan,SUN Yong.et al.Relationship between microecologics and the expression of short chain fatty acids synthesis genes in key bacterial genera in the regulation of intestinal flora structure in populations with constipation and diarrhea[J].Food Sci,2018,39(5):155-165.(in Chinese) 臧凯丽,江岩,孙勇,等.微生态制剂调节便秘、腹泻人群肠道菌群结构与产短链脂肪酸关键菌属的相关性[J].食品科学,2018,39(5):155-165.
[19] PENG Qiannan,HUO Dongxue,XU Chuanbiao,et al.Intestinal microbiota in Li cohort and its correlation with their diets[J].Microbiol China,2017,44(11):2624-2633.(in Chinese) 彭倩楠,霍冬雪,徐传标,等.黎族人肠道微生物群落结构特征及其与饮食关联性[J].微生物学通报,2017,44(11):2624-2633.