地参总黄酮的提取及抗氧化活性研究
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摘要
以总黄酮提取率为指标,在单因素试验基础上,考察乙醇体积分数、料液比、提取温度及提取时间因素,响应面优化地参总黄酮的最佳提取工艺.同时测定地参总黄酮对DPPH·自由基的清除能力.结果显示最适提取工艺为乙醇体积分数60%,料液比1∶60 g/mL,提取温度70℃,提取时间2.5 h,总黄酮提取率为(9.08±0.91)%.地参总黄酮清除DPPH·自由基的IC_(50)为46 mg/L,作用优于V_C.该工艺稳定可行,可有效富集地参中黄酮类化合物,可进一步开发及利用.
To investigate the extract on total flavonoids from Lycopus lucidus Turcz(TFLL) by response surface methodology(RSM), and evaluate its in vitro antioxidant activity. Taking the yield of total flavonoids as indicator, using single factor test, the best extraction process of TFLL was optimized by RSM on the factors of ethanol volume fraction, liquid-solid ratio, extraction temperature and extraction time. Meanwhile, the scavenging effects of TFLL on superoxide free DPPH· were determined. The results showed that the best extraction process of TFLL was that 60% for the concentration of ethanol, 1∶60 g/mL for the ratio of material to liquid, 70 ℃ for extracting temperature, and 2.5 h for time. The yield of total flavonoids was(9.08±0.91)%. The extract exhibited scavenging potential with IC_(50) value of 46 mg/L for DPPH·, which of TFLL was better than that of V_C. The optimized process of TFLL is stable and feasible, that achieved purpose of enrichment of the target of extracting TFLL.
To investigate the extract on total flavonoids from Lycopus lucidus Turcz(TFLL) by response surface methodology(RSM), and evaluate its in vitro antioxidant activity. Taking the yield of total flavonoids as indicator, using single factor test, the best extraction process of TFLL was optimized by RSM on the factors of ethanol volume fraction, liquid-solid ratio, extraction temperature and extraction time. Meanwhile, the scavenging effects of TFLL on superoxide free DPPH· were determined. The results showed that the best extraction process of TFLL was that 60% for the concentration of ethanol, 1∶60 g/mL for the ratio of material to liquid, 70 ℃ for extracting temperature, and 2.5 h for time. The yield of total flavonoids was(9.08±0.91)%. The extract exhibited scavenging potential with IC_(50) value of 46 mg/L for DPPH·, which of TFLL was better than that of V_C. The optimized process of TFLL is stable and feasible, that achieved purpose of enrichment of the target of extracting TFLL.
引文
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[2] 朱苗, 李刚凤, 谢勇, 等. [J]. 广州化工, 2016, 44(17): 20-21+46.
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[4] 常徽. 植物黄酮抗肿瘤效应的结构-效应关系及ROS相关作用机制研究[D]. 重庆: 第三军医大学, 2008.
[5] XIA X, CAO J G, ZHENG Y X, et al. [J]. Indl Crop Prod, 2014, 58(1): 91-98.
[6] WANG X, WANG M L, CAO J G, et al. [J]. Ind Crop Prod, 2017, 97: 137-145.
[7] CHANET A, MILENKOVIC D, DEVAL C, et al. [J]. J Nutr Biochem, 2012, 23(5): 469-477.
[8] 王文净, 高春燕. [J]. 食品研究与开发, 2016, 37(13): 19-22.
[9] 陈贵元, 张翠香, 罗永会, 等. [J]. 中国实验方剂学杂志, 2011, 17(21): 38-40.
[10] 罗永会, 张翠香, 徐春萍. [J]. 食品研究与开发, 2014, 35(7): 88-91.
[11] 丁宁. 红松松子壳多糖的提取纯化、组成分析及抗氧化活性研究[D]. 青岛: 青岛科技大学, 2014.
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[13] 吴清韩, 李云, 朱慧, 等. [J]. 食品工业科技, 2017, 38(10): 1-8.
[14] 霍宗庆, 徐婉晴, 刘言娟. [J]. 食品工业科技, 2017, 38(21): 145-149.
[15] 向莉, 陈轩, 胡艳, 等. [J]. 食品工业科技, 2017, 38(21): 173-177.
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[19] 李月, 王昀, 左绍远, 等. [J]. 食品科技, 2017, 42(12): 191-195.