四级浓香菜籽油酶法脱胶工艺条件优化
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摘要
选取磷脂酶PLA_1,对四级浓香菜籽油进行酶法脱胶工艺研究。分别研究了酶添加量、50%柠檬酸添加量、去离子水添加量、酶解时间对四级浓香菜籽油酶法脱胶效果的影响。在单因素实验的基础上,采用响应面实验设计,对酶法脱胶工艺条件进行优化。结果表明:四级浓香菜籽油酶法脱胶最佳工艺条件为水浴温度55℃、酸反应时间3 min、酶添加量22 mg/kg、50%柠檬酸添加量260mg/kg、去离子水添加量2%、酶解时间120 min,在该条件下酶法脱胶油磷脂含量为22. 26 mg/kg,酸价(KOH)为2. 31 mg/g,收率为96. 81%。
The enzymatic degumming process of four grade fragrant rapeseed oil using phospholipase A_1 was studied. Effects of enzyme dosage,50% citric acid dosage,deionized water dosage and enzymolysis time on enzymatic degumming of four grade fragrant rapeseed oil were investigated. On the basis of single factor experiment,the enzymatic degumming conditions of four grade fragrant rapeseed oil were optimized by response surface methodology. The results showed that the optimal enzymatic degumming conditions were determined as follows: water bath temperature 55 ℃,acid reaction time 3 min,enzyme dosage 22 mg/kg,50% citric acid dosage 260 mg/kg,deionized water dosage 2% and enzymolysis time 120 min.Under these conditions,the phospholipid content of enzymatic degummed oil was 22. 26 mg/kg,the acid value was 2. 31 mgKOH/g,and the yield was 96. 81%.
The enzymatic degumming process of four grade fragrant rapeseed oil using phospholipase A_1 was studied. Effects of enzyme dosage,50% citric acid dosage,deionized water dosage and enzymolysis time on enzymatic degumming of four grade fragrant rapeseed oil were investigated. On the basis of single factor experiment,the enzymatic degumming conditions of four grade fragrant rapeseed oil were optimized by response surface methodology. The results showed that the optimal enzymatic degumming conditions were determined as follows: water bath temperature 55 ℃,acid reaction time 3 min,enzyme dosage 22 mg/kg,50% citric acid dosage 260 mg/kg,deionized water dosage 2% and enzymolysis time 120 min.Under these conditions,the phospholipid content of enzymatic degummed oil was 22. 26 mg/kg,the acid value was 2. 31 mgKOH/g,and the yield was 96. 81%.
引文
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[13]孙万成.固定化磷脂酶A1、酶法改性卵磷脂及溶血磷脂微乳化性能研究[D].广州:暨南大学,2004.
[14]JIANG X F,CHANG M,WANG X,et al.A comparative study of phospholipase A1and phospholipase C on soybean oil degumming[J].J Am Oil Chem Soc,2014,91(12):2125-2134.
[2]何东平,闫子鹏.油脂精炼与加工工艺学[M].北京:化学工业出版社,2012:33-35.
[3]蒋晓菲.磷脂对食用油品质的影响及酶法脱胶技术的研究[D].江苏无锡:江南大学,2015.
[4]万楚筠,黄凤洪,李文林,等.超声波对磷脂酶A1脱除油中非水化磷脂的影响[J].中国油脂,2007,32(2):33-36.
[5]徐赢华,王国敬,李春,等.酶法脱胶在植物油脂精炼中的应用进展[J].农业工程学报,2015,31(23):269-276.
[6]DIJKSTRA A J.Enzymatic degumming[J].Eur J Lipid Sci Technol,2010(11):1178-1189.
[7]薛正莲,司武阳,张珂.磷脂酶A1及其在植物油脱胶中的研究进展[J].粮油食品科技,2009,17(6):26-30.
[8]梁丽,常明,刘睿杰,等.磷脂酶研究进展[J].食品工业科技,2013,34(4):393-396.
[9]JIANG F,WANG J,KALEEM I,et al.Degumming of vegetable oils by a novel phospholipase B from Pseudomonas fluorescens BIT-18[J].Bioresour Technol,2011,102(17):8052-8056.
[10]杨亚济.大豆油酶法脱胶应用实践[J].中国油脂,2016,41(8):107-109.
[11]段书平.大豆油酶法脱胶的生产应用[J].中国油脂,2013,38(8):11-13.
[12]徐振山,郑有涛,刘宝珍.磷脂酶C在大豆油脱胶中的应用实践[J].中国油脂,2017,42(11):152-153.
[13]孙万成.固定化磷脂酶A1、酶法改性卵磷脂及溶血磷脂微乳化性能研究[D].广州:暨南大学,2004.
[14]JIANG X F,CHANG M,WANG X,et al.A comparative study of phospholipase A1and phospholipase C on soybean oil degumming[J].J Am Oil Chem Soc,2014,91(12):2125-2134.