不同产地红枣多糖抗氧化性比较
|
摘要
超声波辅助提取红枣多糖,并通过体外抗氧化体系研究来自不同产地的红枣多糖的抗氧化活性,结果表明,来自横山、绥德、米脂的红枣多糖对羟基自由基、超氧阴离子和DPPH·自由基的清除率都随着多糖浓度的升高而呈上升趋势,不同产地的红枣多糖的清除能力不同,其中来源于横山的红枣多糖对羟基自由基和DPPH自由基的最大清除率均为最好,分别达57.71%和85.09%,来自绥德的红枣多糖对超氧阴离子的最大清除率最好,达61.37%,来自米脂的红枣次之,来自绥德的红枣清除率较低。因此,红枣多糖对超氧阴离子,羟基自由基和DPPH自由基都有较好的清除效果,是具有开发前景的天然抗氧化剂。
Ultrasonic assisted extraction of jujube polysaccharides was carried out, and antioxidant activities of polysaccharides in jujubes from different habitats were studied by the antioxidant system in vitro. The results showed that the scavenging rates of polysaccharides in jujubes from Hengshan, Suide and Mizhi on hydroxyl radicals,superoxide anions and DPPH free radicals increased with the increase of polysaccharide concentration. The maximum scavenging ability of polysaccharides in jujubes from different habitats was different. The maximum scavenging rates of polysaccharides in the jujube from Hengshan to hydroxyl radicals and DPPH free radicals were the best, 57.71%and 85.09%. The maximum scavenging rate of polysaccharides in the jujube from Suide to superoxide anion was the best, up to 61.37%, that from Mizhi was the next, and that from Hengshan was the lowest. Therefore, the jujube polysaccharide has good scavenging effect on superoxide anion, hydroxyl radical and DPPH free radical, and is a natural antioxidant with development prospect.
Ultrasonic assisted extraction of jujube polysaccharides was carried out, and antioxidant activities of polysaccharides in jujubes from different habitats were studied by the antioxidant system in vitro. The results showed that the scavenging rates of polysaccharides in jujubes from Hengshan, Suide and Mizhi on hydroxyl radicals,superoxide anions and DPPH free radicals increased with the increase of polysaccharide concentration. The maximum scavenging ability of polysaccharides in jujubes from different habitats was different. The maximum scavenging rates of polysaccharides in the jujube from Hengshan to hydroxyl radicals and DPPH free radicals were the best, 57.71%and 85.09%. The maximum scavenging rate of polysaccharides in the jujube from Suide to superoxide anion was the best, up to 61.37%, that from Mizhi was the next, and that from Hengshan was the lowest. Therefore, the jujube polysaccharide has good scavenging effect on superoxide anion, hydroxyl radical and DPPH free radical, and is a natural antioxidant with development prospect.
引文
[1]刘广运.大力推进我国红枣产业化——在中国经济林协会红枣产业化座谈会上的讲话[J].中国林业,1999(11):2-3.
[2]雷昌贵,陈锦屏,卢大新.红枣的营养成分及其保健功能[J].现代生物医学进展,2006(03):56-57+62.
[3]王军,张宝善,陈锦屏.红枣营养成分及其功能的研究[J].食品研究与开发,2003(02):68-72.
[4]常江,王陆一,乌兰,等.红枣注射液对小鼠免疫功能的影响[J].包头医学院学报,2002(02):87-88.
[5]王昊,晏玉,谢娅鑫,等.甘薯多糖的提取工艺研究[J].哈尔滨商业大学学报(自然科学版),2017,33(06):698-701.
[6]喻晶,刘宏.低剂量辐射过程中的自由基损伤及药物防护[J].中国医院药学杂志,2011,31(13):1121-1123.
[7]罗依扎·瓦哈甫,骆新,谢飞,等.红枣多糖对小鼠血糖及血清胰岛素水平影响的初步研究[J].食品工业科技,2012,33(22):369-371+374.
[8]李雪华,龙盛京.大枣多糖的提取与抗活性氧研究[J].广西科学,2000(01):54-56+63.
[9]张庆,雷林生,林勤保,等.大枣多糖体外抗补体活性及促进小鼠脾细胞的增殖作用[J].中药药理与临床,1998(05):20-22.
[10]张庆,雷林生,孙莉莎,等.大枣多糖体外对小鼠腹腔巨噬细胞功能的影响[J].中药药理与临床,1999(03):22-24.
[11]张庆,雷林生,杨淑琴,等.大枣中性多糖对小鼠脾淋巴细胞增殖的影响[J].第一军医大学学报,2001(06):426-428.
[12]李金凤,段玉清,马海乐,等.板栗壳中多酚的提取及体外抗氧化性研究[J].林产化学与工业,2010,30(01):53-58.
[13]李霄,陈慧,周苗苗.马铃薯皮中多酚类物质的抗氧化性研究[J].当代化工,2017,46(08):1509-1512.
[14]田应娟,朱良,陈健,等.超声强化提取橄榄黄酮类物质及其抗氧化活性研究[J].食品研究与开发,2011,32(01):17-22.
[15]李霄,曹艳萍,马向荣,等.陕北红枣多酚抗氧化性研究与比较[J].应用化工,2012,41(08):1368-1371.
[16]朱丹,牛广财,蔡亚平,等.沙果渣多酚抗氧化活性的研究[J].中国食品添加剂,2010(05):129-132.
[17]张宽朝,李冬磊,陈曼曼,等.甘薯渣多酚粗提液的抗氧化活性研究[J].食品工业科技,2011,32(03):163-165.
[18]宋丽,甄萍萍,李大红.香天竺葵精油抗氧化活性研究[J].河南农业科学,2011,40(02):135-137.
[19]王玉丽,任海伟,李志忠,等.用清除DPPH自由基法评价药黑豆色素的抗氧化能力[J].食品工业科技,2009(8):102-105.
[20]杨孝延,汪道兵,孙玉军.冬瓜多糖的超声波辅助提取及其抗氧化活性研究[J].江汉大学学报(自然科学版),2017,45(06):530-536.
[2]雷昌贵,陈锦屏,卢大新.红枣的营养成分及其保健功能[J].现代生物医学进展,2006(03):56-57+62.
[3]王军,张宝善,陈锦屏.红枣营养成分及其功能的研究[J].食品研究与开发,2003(02):68-72.
[4]常江,王陆一,乌兰,等.红枣注射液对小鼠免疫功能的影响[J].包头医学院学报,2002(02):87-88.
[5]王昊,晏玉,谢娅鑫,等.甘薯多糖的提取工艺研究[J].哈尔滨商业大学学报(自然科学版),2017,33(06):698-701.
[6]喻晶,刘宏.低剂量辐射过程中的自由基损伤及药物防护[J].中国医院药学杂志,2011,31(13):1121-1123.
[7]罗依扎·瓦哈甫,骆新,谢飞,等.红枣多糖对小鼠血糖及血清胰岛素水平影响的初步研究[J].食品工业科技,2012,33(22):369-371+374.
[8]李雪华,龙盛京.大枣多糖的提取与抗活性氧研究[J].广西科学,2000(01):54-56+63.
[9]张庆,雷林生,林勤保,等.大枣多糖体外抗补体活性及促进小鼠脾细胞的增殖作用[J].中药药理与临床,1998(05):20-22.
[10]张庆,雷林生,孙莉莎,等.大枣多糖体外对小鼠腹腔巨噬细胞功能的影响[J].中药药理与临床,1999(03):22-24.
[11]张庆,雷林生,杨淑琴,等.大枣中性多糖对小鼠脾淋巴细胞增殖的影响[J].第一军医大学学报,2001(06):426-428.
[12]李金凤,段玉清,马海乐,等.板栗壳中多酚的提取及体外抗氧化性研究[J].林产化学与工业,2010,30(01):53-58.
[13]李霄,陈慧,周苗苗.马铃薯皮中多酚类物质的抗氧化性研究[J].当代化工,2017,46(08):1509-1512.
[14]田应娟,朱良,陈健,等.超声强化提取橄榄黄酮类物质及其抗氧化活性研究[J].食品研究与开发,2011,32(01):17-22.
[15]李霄,曹艳萍,马向荣,等.陕北红枣多酚抗氧化性研究与比较[J].应用化工,2012,41(08):1368-1371.
[16]朱丹,牛广财,蔡亚平,等.沙果渣多酚抗氧化活性的研究[J].中国食品添加剂,2010(05):129-132.
[17]张宽朝,李冬磊,陈曼曼,等.甘薯渣多酚粗提液的抗氧化活性研究[J].食品工业科技,2011,32(03):163-165.
[18]宋丽,甄萍萍,李大红.香天竺葵精油抗氧化活性研究[J].河南农业科学,2011,40(02):135-137.
[19]王玉丽,任海伟,李志忠,等.用清除DPPH自由基法评价药黑豆色素的抗氧化能力[J].食品工业科技,2009(8):102-105.
[20]杨孝延,汪道兵,孙玉军.冬瓜多糖的超声波辅助提取及其抗氧化活性研究[J].江汉大学学报(自然科学版),2017,45(06):530-536.