一种离轴数字全息显微相位自动补偿方法
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摘要
在离轴数字全息显微技术中,离轴光路往往在数字参考光重建过程中导致倾斜的相位畸变,严重影响重建质量.采用一种畸变相位自动补偿的方法,对数字全息重建过程进行研究.通过对全息图的频域图进行处理,找到+1级像和零级像所成角度的对应关系,利用多像素坐标计算,得到相应的倾斜数字参考光.利用倾斜的数字参考光进行数字重建,再通过对重建相位图进行二值化处理,利用最大连通区域判定找到最佳补偿相位,实现相位的自动补偿.实验证明方法效果较好,能实现自动消除一次相位畸变的功能.
In the off-axis digital holographic microscopy,off-axis optical paths tend to cause tilted phase distortion during the digital reference light reconstruction,which seriously affects the quality of the reconstruction. A method of automatic distortion compensation is proposed to study the digital holographic reconstruction process. By processing the frequency domain of the hologram,the corresponding relationship between the angle of the first term and the DC term is found,and the multi-pixel coordinate calculation is used to obtain the corresponding tilted digital reference light. The digital reconstruction is performed using the tilted digital reference light,then the reconstructed phase is binarized,and the optimal compensation phase is found using the maximum connected region to realize the automatic phase compensation. The experiment proves that this method is effective and can realize the automatically eliminating tilt phase distortion.
In the off-axis digital holographic microscopy,off-axis optical paths tend to cause tilted phase distortion during the digital reference light reconstruction,which seriously affects the quality of the reconstruction. A method of automatic distortion compensation is proposed to study the digital holographic reconstruction process. By processing the frequency domain of the hologram,the corresponding relationship between the angle of the first term and the DC term is found,and the multi-pixel coordinate calculation is used to obtain the corresponding tilted digital reference light. The digital reconstruction is performed using the tilted digital reference light,then the reconstructed phase is binarized,and the optimal compensation phase is found using the maximum connected region to realize the automatic phase compensation. The experiment proves that this method is effective and can realize the automatically eliminating tilt phase distortion.
引文
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[10] Martyniuk M. Low-temperature micro-opto-electromechanical technologies for temperature sensitive substrates[D]. Perth:University of Western Australia,2006.
[2] Majeed H,Sridharan S,Mir M,et al. Quantitative phase imaging for medical diagnosis[J]. Journal of Biophotonics,2017, 10(2):177-205.
[3] Marquet P,Depeursinge C,Magistretti P J. Exploring neural cell dynamics with digital holographic microscopy[J].Annual Review of Biomedical Engineering,2013,15(1):407-431.
[4] Fukui K I,Namai Y, Iwasawa Y. Imaging of surface oxygen atoms and their defect structures on CeO2(1 1 1)by noncontact atomic force microscopy[J]. Applied Surface Science, 2002, 188(3/4):252-256.
[5] Zhou W J,Peng J,Yu Y J. Deformation measurement via digital holography[J]. Optics&Precision Engineering,2005, 13(z1):46-51.
[6] Palacios F,Ricardo J,Palacios D, et al. 3D image reconstruction of transparent microscopic objects using digital holography[J]. Optics Communications, 2005,248(1/2/3):41-50.
[7] Mann C J,Kim M K. Quantitative phase-contrast microscopy by angular spectrum digital holography[C]//Proceedings of SPIE-The International Society for Optical Engineering. San Jose,2006:60900B-1-60900B-8.
[8]钟丽云,张以谟,吕晓旭,等,数字全息中的一些基本问题分析[J].光学学报,2004,24(4):465-471.(Zhong Li-yun,Zhang Yi-mo,Lyu Xiao-xu,et al. Analysis of some fundamental issue about digital hologram[J]. Acta Optica Sinica,2004,24(4):465-471.)
[9] Cuche E, Marquet P, Depeursinge C. Simultaneous amplitude-contrast and quantitative phase-contrast microscopy by numerical reconstruction of Fresnel off-axis holograms[J]. Applied Optics, 1999,38(34):6994-7001.
[10] Martyniuk M. Low-temperature micro-opto-electromechanical technologies for temperature sensitive substrates[D]. Perth:University of Western Australia,2006.