不同年份水稻抽穗期和穗部性状QTL分析
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摘要
穗部性状与水稻品种的丰产潜力密切相关,而抽穗期是决定水稻品种能否在一个地区种植的决定因素,了解穗部性状和抽穗期的遗传基础及其与产量性状的关系,对水稻高产育种具有重要意义。以粳稻笹锦和籼稻北陆129杂交、回交衍生的回交重组自交系(BILs)群体为试验材料,对抽穗期和穗部相关性状进行QTL分析。结果表明,抽穗期和穗粒数在亲本间存在显著的差异,且抽穗期与结实率间存在显著负相关。检测到8个控制抽穗期的QTL,其中qHD3、qHD7和qHD8在2年中都被检测到,贡献率介于7.03%~27.48%之间,具有较强的延长抽穗期功能;检测到5个控制颖花数的QTL,其中qGN1a和qGN5在2年里都被检测到,贡献率介于8.37%~20.05%之间;检测到5个控制实粒数的QTL,其中qSN1a是1个稳定表达的主效QTL位点;检测到4个控制结实率的QTL,其中qSSR12是1个稳定表达的主效QTL位点。比较分析发现,qHD1a与qSSR1、qHD12与qSSR12存在遗传重叠效应,qHD1b与qGN1c和qSN1b、qSN2与qSSR2、qSSR6与qGN6和qSN6存在遗传重叠效应,这表明抽穗期和穗粒数是影响籽粒灌浆结实的主要因素。这些QTL将为水稻分子育种提供理论参考。
Panicle traits are closely related to the yield potential of rice varieties, and the heading date is the determining factor of whether rice varieties can be planted in area region. It is important to understand the genetic basis of panicle traits and heading date and its relationship with yield traits. The backcross recombinant inbred lines(BILs) derived from the cross between Sasanisiki(japonica) and Habataki(indica) were used to identify QTLs for heading stage and panicle traits in different environments. The results showed that there were significant differences between heading dates and between grain number per panicle, and there was significant negative correlation between heading date and seed setting rate. A total of eight QTLs were mapped for plant heading stage, among these QTL, qHD3, qHD7 and qHD8 were identified in two years, and they were able to extend the heading date. Five QTLs were mapped for spikelets per panicle, among them, qGN1a and qGN5 were detected in two years. Five QTLs were detected for filled grain number per panicle, qSN1a was a stable expression of the main effect QTL. Four QTLs were detected for seed setting rates, qSSR12 was a stable expression of the main effect QTL. In addition, among them,pleiotropic QTL qHD1a and qSSR1, qHD12 and qSSR12, qHD1b and qGN1c and qSN1b qSN2 and qSSR2, qSSR6 and qGN6 and qSN6 were detected on the same region of chromosome, which indicated that heading date and grains per panicle were the most important factors affecting grain filling. The identification of genes constituting the QTLs will help to further understand the molecular mechanisms underlying heading stage and provide a theoretical reference for the molecular marker assisted selection.
Panicle traits are closely related to the yield potential of rice varieties, and the heading date is the determining factor of whether rice varieties can be planted in area region. It is important to understand the genetic basis of panicle traits and heading date and its relationship with yield traits. The backcross recombinant inbred lines(BILs) derived from the cross between Sasanisiki(japonica) and Habataki(indica) were used to identify QTLs for heading stage and panicle traits in different environments. The results showed that there were significant differences between heading dates and between grain number per panicle, and there was significant negative correlation between heading date and seed setting rate. A total of eight QTLs were mapped for plant heading stage, among these QTL, qHD3, qHD7 and qHD8 were identified in two years, and they were able to extend the heading date. Five QTLs were mapped for spikelets per panicle, among them, qGN1a and qGN5 were detected in two years. Five QTLs were detected for filled grain number per panicle, qSN1a was a stable expression of the main effect QTL. Four QTLs were detected for seed setting rates, qSSR12 was a stable expression of the main effect QTL. In addition, among them,pleiotropic QTL qHD1a and qSSR1, qHD12 and qSSR12, qHD1b and qGN1c and qSN1b qSN2 and qSSR2, qSSR6 and qGN6 and qSN6 were detected on the same region of chromosome, which indicated that heading date and grains per panicle were the most important factors affecting grain filling. The identification of genes constituting the QTLs will help to further understand the molecular mechanisms underlying heading stage and provide a theoretical reference for the molecular marker assisted selection.
引文
[1]胡时开,苏岩,叶卫军,等.水稻抽穗期遗传与分子调控机理研究进展[J].中国水稻科学,2012,26(3):373-382.
[2]岳兵,邢永忠.水稻抽穗期分子遗传研究进展[J].分子植物育种,2005,3(2):222-228.
[3]郭梁,张振华,庄杰云.水稻抽穗期QTL及其与产量性状遗传控制的关系[J].中国水稻科学,2012,26(2):235-245.
[4]冯玉涛,蒋海潮,高冠军,等.利用高世代回交群体定位水稻抽穗期和株高QTL[J].分子植物育种,2014,12(1):1-8.
[5]冯跃,翟荣荣,曹立勇,等.不同施氮水平下水稻株高与抽穗期的QTL比较分析[J].作物学报,2011,37(9):1525-1532.
[6]杨雷,柳絮,吴振映,等.水稻单片段代换系抽穗期QTL鉴定及遗传分析[J].山东农业科学,2011,43(5):4-7.
[7]黄成,姜树坤,刘梦红,等.水稻抽穗期的QTL剖析[J].华北农学报,2009,24(3):7-9.
[8]卢丙越,马孟莉,江玲,等.利用BIL群体定位水稻抽穗期QTL及主效位点遗传效应分析[J].西北农业学报,2014,23(7):51-54.
[9]YAN W,LIU H,ZHOU X,et al.Natural variation in Ghd7 plays an important role in grain yield and adaptation in rice[J].Cell Res,2013,23:969-971.
[10]YAMAMOTO T,LIN H X,SASAKI T,et al.Identification of head ing date quantitative trait locus Hd6 and characterization of its epistatic interactions with Hd2 in rice using advanced backcross progeny[J].Genetics,2000,154:885-891.
[11]YANO M,KATAYOSE Y,ASHIKARI M,et al.Hd1,a major photoperiod sensitivity quantitative trait locus in rice,is closely related to the Arabidopsis flowering line gene[J].Plant Cell,2000,12:2473-2483.
[12]ZHONG Z Z,WU W X,WANG H J,et al.Fine mapping of a minor-effect QTL,DTH12,controlling heading date in rice by upregulation of florigen genes under long-day conditions[J].Mol Breeding,2014,34:311-322.
[13]朱海霞,王秋京,闫平,等.孕穗抽穗期低温处理对黑龙江省主栽水稻品种结实率的影响[J].中国农业气象,2012,33(2):304-309.
[14]WANG J K,LI H H,ZHANG L Y.Users’Manual of QTL Ici Mapping v 4.0[EB/OL].Beijing:Institute of Crop Science,2014.http://www.isbreeding.net.
[15]MCCOUCH S R,CKO Y G,YANO M,et al.Report on QTL nomenclature[J].Rice Genet Newslett,1997,14:11-13.
[16]王军,朱金燕,周勇,等.不同温光条件下水稻抽穗期QTL的定位与分析[J].中国水稻科学,2016,30(3):247-255.
[17]李泽福,周彤,郑天清,等.水稻抽穗期QTL与环境互作分析[J].作物学报,2002,28(6):771-776.
[18]杨德卫,张亚东,朱镇.基于CSSL的水稻抽穗期QTL定位及遗传分析[J].植物学报,2010,45(1):189-197.
[19]周勇,缪军,陶亚军,等.利用染色体单片段代换系定位水稻结实率QTLs[J].扬州大学学报(农业与生命科学版),2013,34(4):45-49.
[20]PEI C G,LIU X,WANG W Y,et al.Fine mapping of q HD8-1,a QTL controlling the heading date,to a 26-kb DNA fragment in rice(Oryza sativa L.)[J].J Plant Biol,2011,54:190-198.
[21]MONNA L,LIN HX,KOJIMA S,et al.Genetic dissection of a genemic region for a quantitative trait locus Hd3,into two loci,Hd3a and Hd3b,controlling heading date in rice[J].Theor Appl Genet,2002,104:772-778.
[22]TAKAHASHI Y,SHOMURA A,SASAKI T,et al.Hd6,a rice quantitative trait locus involved in photoperiod sensitivity,encodes the alpha subunit of protein kinase CK2[J].Proc Natl Acad Sci USA,2001,98:7922-7927.
[23]XUE W Y,XING Y Z,WENG X Y,et al.Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J].Nat Genet,2008,40:761-767.
[24]孙亚伟.水稻染色体单片段代换系农艺性状分析及QTL定位[D].扬州:扬州大学,2009.
[25]刘丹,刘进,王嘉宇,等.利用籼粳交RIL群体对水稻穗部性状的QTL分析[J].沈阳农业大学学报,2013,44(6):727-732.
[26]许凌,张亚东,朱镇,等.不同年份水稻产量性状的QTL分析[J].中国水稻科学,2008,22(4):370-376.
[27]高云,陶亚军,王立广,等.利用染色体单片段代换系定位水稻结实率QTL[J].江苏农业科学,2014,42(11):17-20.
[2]岳兵,邢永忠.水稻抽穗期分子遗传研究进展[J].分子植物育种,2005,3(2):222-228.
[3]郭梁,张振华,庄杰云.水稻抽穗期QTL及其与产量性状遗传控制的关系[J].中国水稻科学,2012,26(2):235-245.
[4]冯玉涛,蒋海潮,高冠军,等.利用高世代回交群体定位水稻抽穗期和株高QTL[J].分子植物育种,2014,12(1):1-8.
[5]冯跃,翟荣荣,曹立勇,等.不同施氮水平下水稻株高与抽穗期的QTL比较分析[J].作物学报,2011,37(9):1525-1532.
[6]杨雷,柳絮,吴振映,等.水稻单片段代换系抽穗期QTL鉴定及遗传分析[J].山东农业科学,2011,43(5):4-7.
[7]黄成,姜树坤,刘梦红,等.水稻抽穗期的QTL剖析[J].华北农学报,2009,24(3):7-9.
[8]卢丙越,马孟莉,江玲,等.利用BIL群体定位水稻抽穗期QTL及主效位点遗传效应分析[J].西北农业学报,2014,23(7):51-54.
[9]YAN W,LIU H,ZHOU X,et al.Natural variation in Ghd7 plays an important role in grain yield and adaptation in rice[J].Cell Res,2013,23:969-971.
[10]YAMAMOTO T,LIN H X,SASAKI T,et al.Identification of head ing date quantitative trait locus Hd6 and characterization of its epistatic interactions with Hd2 in rice using advanced backcross progeny[J].Genetics,2000,154:885-891.
[11]YANO M,KATAYOSE Y,ASHIKARI M,et al.Hd1,a major photoperiod sensitivity quantitative trait locus in rice,is closely related to the Arabidopsis flowering line gene[J].Plant Cell,2000,12:2473-2483.
[12]ZHONG Z Z,WU W X,WANG H J,et al.Fine mapping of a minor-effect QTL,DTH12,controlling heading date in rice by upregulation of florigen genes under long-day conditions[J].Mol Breeding,2014,34:311-322.
[13]朱海霞,王秋京,闫平,等.孕穗抽穗期低温处理对黑龙江省主栽水稻品种结实率的影响[J].中国农业气象,2012,33(2):304-309.
[14]WANG J K,LI H H,ZHANG L Y.Users’Manual of QTL Ici Mapping v 4.0[EB/OL].Beijing:Institute of Crop Science,2014.http://www.isbreeding.net.
[15]MCCOUCH S R,CKO Y G,YANO M,et al.Report on QTL nomenclature[J].Rice Genet Newslett,1997,14:11-13.
[16]王军,朱金燕,周勇,等.不同温光条件下水稻抽穗期QTL的定位与分析[J].中国水稻科学,2016,30(3):247-255.
[17]李泽福,周彤,郑天清,等.水稻抽穗期QTL与环境互作分析[J].作物学报,2002,28(6):771-776.
[18]杨德卫,张亚东,朱镇.基于CSSL的水稻抽穗期QTL定位及遗传分析[J].植物学报,2010,45(1):189-197.
[19]周勇,缪军,陶亚军,等.利用染色体单片段代换系定位水稻结实率QTLs[J].扬州大学学报(农业与生命科学版),2013,34(4):45-49.
[20]PEI C G,LIU X,WANG W Y,et al.Fine mapping of q HD8-1,a QTL controlling the heading date,to a 26-kb DNA fragment in rice(Oryza sativa L.)[J].J Plant Biol,2011,54:190-198.
[21]MONNA L,LIN HX,KOJIMA S,et al.Genetic dissection of a genemic region for a quantitative trait locus Hd3,into two loci,Hd3a and Hd3b,controlling heading date in rice[J].Theor Appl Genet,2002,104:772-778.
[22]TAKAHASHI Y,SHOMURA A,SASAKI T,et al.Hd6,a rice quantitative trait locus involved in photoperiod sensitivity,encodes the alpha subunit of protein kinase CK2[J].Proc Natl Acad Sci USA,2001,98:7922-7927.
[23]XUE W Y,XING Y Z,WENG X Y,et al.Natural variation in Ghd7 is an important regulator of heading date and yield potential in rice[J].Nat Genet,2008,40:761-767.
[24]孙亚伟.水稻染色体单片段代换系农艺性状分析及QTL定位[D].扬州:扬州大学,2009.
[25]刘丹,刘进,王嘉宇,等.利用籼粳交RIL群体对水稻穗部性状的QTL分析[J].沈阳农业大学学报,2013,44(6):727-732.
[26]许凌,张亚东,朱镇,等.不同年份水稻产量性状的QTL分析[J].中国水稻科学,2008,22(4):370-376.
[27]高云,陶亚军,王立广,等.利用染色体单片段代换系定位水稻结实率QTL[J].江苏农业科学,2014,42(11):17-20.