291份燕麦种质资源对红叶病抗性的鉴定
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摘要
为明确不同燕麦种质抗大麦黄矮病毒差异,于2011～2016年采用人工接种对291份燕麦种质进行了由大麦黄矮病毒(Barley yellow dwarf virus,BYDV)引起的燕麦红叶病的田间抗性鉴定。结果表明:在供试的204份皮燕麦(Hulled oats)材料中,未检测出免疫材料;其中DA92-2F4,DA92-3F4和Rigdon等3份材料表现高抗(HR),占供试材料的1.5%;Wallaroo、青永久96、青23、青永久709等11份材料表现为抗(R),占5.4%;青3115、131-10燕麦、S20-36-6、DA92-1F4、青永久304等42份材料表现中抗(MR),占20.6%;45份和103份材料分别表现感(S)和高感(HS),占22.1%和50.5%。在供试的87份裸燕麦(Naked oats)材料中,未检测到免疫材料,其中VAO-1,定莜2032和花早2号等3份材料表现高抗(HR),占3.4%;2002x2-2-5-1-5-16、200215-13-2-2、9641-6、9418和08行-171-22等5份材料表现抗(R),占5.7%;白燕2号、白燕11号、内燕4号、07-21-7、定西R-30-21燕麦等28份材料表现为中抗(MR),占32.2%;36份和15份材料分别表现感(S)和高感(HS),占41.4%和17.2%。6份燕麦品种(系)首次被鉴定为高抗BYDV材料,可为燕麦种质的合理布局和抗病育种提供核心抗源。
In order to clarify the difference of different oats germplasm resistance to red leaf disease,the identification and evaluation of 291 oats germplasm resources resistance to red leaf disease caused by barley yellow dwarf virus(BYDV) was carried out by artificial inoculation in field from 2011 to 2016.The results showed that the immune material was not detected in 204 hulled oats-tested.Three materials,DA92-2 F4,DA92-3 F4 and Rigdon were highly resistant,which accounted for 1.5% of 204 hulled oats.Eleven materials,such as Wallaroo,Qingyongjiu 96,Qing 23,Qingyongjiu 709 were resistant,by 5.4% of 204 hulled oats.A total of 42 materials,such as Qing 3115,oat 131-10,S20-36-6,DA92-1 F4,Qingyongjiu 304 were moderately resistant,by 20.6% of 204 hulled oats.A total of 45 and 103 materials were susceptible and highly susceptible,which accounted for 22.1% and 50.5% of 204 hulled oats,respectively.The immune material was not detected in 87 naked oats-tested.Three materials,VOA-1,Dingyou2032 and Huazao 2 were highly resistant,which accounted for 3.4% of 87 naked oats.Five materials,such as 2002 x2-2-5-1-5-16,200215-13-2-2,9641-6,9418 and 08 line-171-22 were resistant,by 5.7% of 87 naked oats.A total of 28 materials,such as Baiyan 2,Baiyan11,Neiyan 4,07-21-7,Dingxi R-30-21 were moderately resistant,by 32.2% of 87 naked oats.A total of 36 and 15 materials were susceptible and highly susceptible,which accounted for 41.4% and 17.2% of 87 naked oats,respectively.Six materials were first identified high resistance.Obtaining these effective resistance germplasms could provide core resistance source for breeding and cultivars of reasonable layout.
In order to clarify the difference of different oats germplasm resistance to red leaf disease,the identification and evaluation of 291 oats germplasm resources resistance to red leaf disease caused by barley yellow dwarf virus(BYDV) was carried out by artificial inoculation in field from 2011 to 2016.The results showed that the immune material was not detected in 204 hulled oats-tested.Three materials,DA92-2 F4,DA92-3 F4 and Rigdon were highly resistant,which accounted for 1.5% of 204 hulled oats.Eleven materials,such as Wallaroo,Qingyongjiu 96,Qing 23,Qingyongjiu 709 were resistant,by 5.4% of 204 hulled oats.A total of 42 materials,such as Qing 3115,oat 131-10,S20-36-6,DA92-1 F4,Qingyongjiu 304 were moderately resistant,by 20.6% of 204 hulled oats.A total of 45 and 103 materials were susceptible and highly susceptible,which accounted for 22.1% and 50.5% of 204 hulled oats,respectively.The immune material was not detected in 87 naked oats-tested.Three materials,VOA-1,Dingyou2032 and Huazao 2 were highly resistant,which accounted for 3.4% of 87 naked oats.Five materials,such as 2002 x2-2-5-1-5-16,200215-13-2-2,9641-6,9418 and 08 line-171-22 were resistant,by 5.7% of 87 naked oats.A total of 28 materials,such as Baiyan 2,Baiyan11,Neiyan 4,07-21-7,Dingxi R-30-21 were moderately resistant,by 32.2% of 87 naked oats.A total of 36 and 15 materials were susceptible and highly susceptible,which accounted for 41.4% and 17.2% of 87 naked oats,respectively.Six materials were first identified high resistance.Obtaining these effective resistance germplasms could provide core resistance source for breeding and cultivars of reasonable layout.
引文
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[4] 李润枝,陈晨,张培培,等.我国燕麦种质资源与遗传育种研究进展[J].现代农业科技,2009(17):44-45.
[5] 郑殿升.中国燕麦的多样性[J].植物遗传资源学报,2010,10(3):249-252.
[6] The American Phytopathological Society[EB/OL].(1993-03-11)[2017-01-22].http://www.apsnet.org /publications /commonnames /Pages/Oats.
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[8] 刘刚,赵桂琴,杨成德,等.青藏高原燕麦品种生物抗性初探[J].草地学报,2007,15(6):582-587.
[9] 郭建国,郭满库,郭成,等.燕麦抗蚜性和抗 BYDV 病毒病鉴定及利用评价[J].中国农业科学,2012,45(3):575-583.
[10] 胡凯军.抗红叶病燕麦种质评价和筛选[D].兰州:甘肃农业大学硕士论文,2010.
[11] 郭建国,袁伟宁,赵彤.燕麦抗红叶病研究进展[J].甘肃农业科技,2017(11):69-71.
[12] 郭成,王艳,张新瑞,等.燕麦种质抗坚黑穗病鉴定与评价[J].草地学报,2017,25(2):379-386.
[13] 中华人民共和国农业部.中华人民共和国农业行业标准NY/T 3060.6-2016.大麦品种抗病性鉴定技术规程第6部分:抗黄矮病[S].北京:中国农业出版社,2016:3107-3114.
[14] 周青平.高原燕麦的栽培与管理[M].南京:江苏凤凰科学技术出版社,2014.
[15] 李春杰,陈泰祥,赵桂琴,等.燕麦病害研究进展[J].草业学报,2017,26(12):203-222.
[16] Zhu S,Kolb F L,Kaeppler H F.Molecular mapping of genomic regions underlying barley yellow dwarf tolerance in cultivated oat(Avena sativa L.) [J].Theoretical and Applied Genetics,2003,106(7):1300-1306.
[2] 杨海鹏,孙泽民.中国燕麦[M].北京:农业出版社,1989:7-10.
[3] 郑殿升,张宗文.中国燕麦种质资源国外引种与利用[J].植物遗传资源学报,2017,18(6):1001-1005.
[4] 李润枝,陈晨,张培培,等.我国燕麦种质资源与遗传育种研究进展[J].现代农业科技,2009(17):44-45.
[5] 郑殿升.中国燕麦的多样性[J].植物遗传资源学报,2010,10(3):249-252.
[6] The American Phytopathological Society[EB/OL].(1993-03-11)[2017-01-22].http://www.apsnet.org /publications /commonnames /Pages/Oats.
[7] 郭忠贤,刘英,王秀琴,等.燕麦红叶病发生规律的研究[J].内蒙古农业科技,1998(5):18-19.
[8] 刘刚,赵桂琴,杨成德,等.青藏高原燕麦品种生物抗性初探[J].草地学报,2007,15(6):582-587.
[9] 郭建国,郭满库,郭成,等.燕麦抗蚜性和抗 BYDV 病毒病鉴定及利用评价[J].中国农业科学,2012,45(3):575-583.
[10] 胡凯军.抗红叶病燕麦种质评价和筛选[D].兰州:甘肃农业大学硕士论文,2010.
[11] 郭建国,袁伟宁,赵彤.燕麦抗红叶病研究进展[J].甘肃农业科技,2017(11):69-71.
[12] 郭成,王艳,张新瑞,等.燕麦种质抗坚黑穗病鉴定与评价[J].草地学报,2017,25(2):379-386.
[13] 中华人民共和国农业部.中华人民共和国农业行业标准NY/T 3060.6-2016.大麦品种抗病性鉴定技术规程第6部分:抗黄矮病[S].北京:中国农业出版社,2016:3107-3114.
[14] 周青平.高原燕麦的栽培与管理[M].南京:江苏凤凰科学技术出版社,2014.
[15] 李春杰,陈泰祥,赵桂琴,等.燕麦病害研究进展[J].草业学报,2017,26(12):203-222.
[16] Zhu S,Kolb F L,Kaeppler H F.Molecular mapping of genomic regions underlying barley yellow dwarf tolerance in cultivated oat(Avena sativa L.) [J].Theoretical and Applied Genetics,2003,106(7):1300-1306.